LOCUS       Exported                6680 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     62..81
                     /label=T7
     primer_bind     101..140
                     /label=CVP434
     primer_bind     111..169
                     /label=CVP679
     misc_feature    142..1425
                     /label=YebS
     CDS             142..1425
                     /label=Translation 142-1425
     misc_feature    142..169
                     /label=29 / 59.7
     primer_bind     589..607
                     /label=oDCE3554
     misc_feature    840
                     /label=T->C mutation relative to reference sequence
     misc_feature    843
                     /label=T->A mutation relative to reference sequence
     misc_feature    1113
                     /label=A->C mutation relative to reference sequence
     primer_bind     1195..1215
                     /label=oDCE3555
     primer_bind     1328..1349
                     /label=CVP673
     CDS             1394..3634
                     /label=Translation 1394-3634
     misc_feature    1394..1425
                     /label=YebT
     CDS             1426..3634
                     /label=MCE domain protein
     CDS             1426..3634
                     /label=yebT
     misc_feature    1451..1513
                     /label=TM helix (TOPCONS)
     misc_feature    1526..1804
                     /label=MCE domain (CDD)
     primer_bind     1529..1558
                     /label=CVP676
     primer_bind     complement(1596..1618)
                     /label=CVP675
     misc_feature    1868..2113
                     /label=MCE domain (weak/partial hit, CDD)
     primer_bind     1963..1984
                     /label=CVP640
     misc_feature    2228..2500
                     /label=MCE domain (CDD)
     misc_feature    2564..2803
                     /label=MCE domain (weak/partial hit, CDD)
     primer_bind     2564..2582
                     /label=CVP641
     STS             2700..2985
                     /label=STS
     misc_feature    3290..3565
                     /label=MCE domain (CDD)
     misc_feature    3596..3631
                     /label=mce6
     CDS             3605..3679
                     /label=Translation 3605-3679
     primer_bind     complement(3605..3679)
                     /label=oSTN003 ring6 reverse
     primer_bind     complement(3605..3679)
                     /label=CVP680
     misc_feature    3629..3631
                     /label=MCE domain (CDD)
     terminator      3714..3760
                     /label=T7 terminator
     primer_bind     complement(3793..3812)
                     /label=oDCE3649
     CDS             4194..4853
                     /label=AmpR
     rep_origin      4951..5633
                     /label=ColE1 origin
     primer_bind     complement(5341..5399)
                     /label=CVP347
     primer_bind     5524..5547
                     /label=CVP669
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
      181 ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa
      241 tgtgacatgc tgtttagcct gcccgagata aattctcatc aaagtgccta ttgtccgcgc
      301 tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc
      361 ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac cgctgttgca tatctggctg
      421 ttaggcattc gtattgacgc caacgttatg caaggcatct ggcaaatgac caaacagggc
      481 gatgcgataa cggggtcgat ggtctttttc tgcgttattg gtgccccgct cattctggtg
      541 acctccatag cttatttatg gtttggtaac cgactgggaa tgaatttacg tccggtactg
      601 ctgatgcttg agcgacttaa agagtgggta atgctcgata tctacctggt cggcattggc
      661 gttgcttcta taaaggtaca ggattatgcc catatccagg ctggtgtcgg cttgttctct
      721 tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt cgaagaactg
      781 tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact tcgtgtctgc
      841 ctagggtgcc attttaccgg ctatccagat cagcgtggtc gctgcccgcg ttgccatatc
      901 ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct gggcggcgct gttagcgtca
      961 atcgttttat tgttacctgc caacctgttg cctatttcta tcatttatct gaacggagga
     1021 cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa cattgcggtt
     1081 gcaggaatcg tgtttatcgc cagtattctg gtcccgttta ctaaagtgat cgtcatgttc
     1141 actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg cattctgtta
     1201 ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt catatcttta
     1261 accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc ggctgcgttt
     1321 tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga cagccgctta
     1381 ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa
     1441 taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga ttgccagttg
     1501 gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact ttatgtcggc
     1561 ggatggtatt gttccaggcc gtacgcctgt tcgttatcag ggcgttgaag tcggaacagt
     1621 gcaggatatc agcctcagcg acgatcttcg taagattgaa gtcaaggtca gcatcaagtc
     1681 cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg ctggtgacgc caaaagcatc
     1741 gttggcaggt gtctccgggc tggacgccct cgtcggtggg aactatatcg gcatgatgcc
     1801 gggtaaaggt aaagagcagg atcactttgt cgcactcgat acccaaccga aatatcggct
     1861 ggacaatggc gatctgatga tccacctgca agcccccgat ctcggttcgc tgaacagcgg
     1921 ttcattggtc tatttccgca agatcccggt gggaaaagtc tacgactatg ccatcaatcc
     1981 caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg cgttttaccg atctggtgaa
     2041 aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc aacgtcagta tcagtggcgc
     2101 gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt gcgattgcct tcgattcacc
     2161 agaagagtcg aaacctgccg aggcggaaga tacctttggt ctgtatgaag atctggccca
     2221 cagccagcgt ggcgtaataa taaaactgga actgccgagt ggggccggat taaccgccga
     2281 ctcgacgccg ttaatgtatc aggggctgga agtcggacag ctgactaaac tggatttaaa
     2341 tcctggtggt aaagtcacag gggaaatgac cgttgatccc agcgtcgtta ccctgcttcg
     2401 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
     2461 cgccctgctg accggcaaaa ccttcgagct ggtgcccggc gatggcgagc cacgcaaaga
     2521 gttcgttgtt gtgccaggcg aaaaagcact gctgcatgaa cctgatgttc tgacgctgac
     2581 cctgaccgcg ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
     2641 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
     2701 catcgagcct cagcatcgcg aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
     2761 tgtcgatgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
     2821 gatcaatggc gggatacgta ttctgccggg cgataaaggt gagatgaaag ccagctatcc
     2881 actgtatgcc aatttggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
     2941 cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
     3001 taaatttgaa gttggtgaag tgattaccgt gcgtccgcga gctaacgcgt ttgatatcga
     3061 tctgcatatt aagccggagt atcgcaacct tctgaccagc aatagcgtgt tctgggcaga
     3121 aggcggggcg aaagttcagc tgaatggtag tggtctgacc gtacaggcat ccccgctctc
     3181 cagagcatta aagggagcca ttagcttcga caacctcagc ggtgccagcg ccagtcagcg
     3241 taaaggcgat aagcgtattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcgggca
     3301 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
     3361 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
     3421 ggcaaaggcg gtactttacc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
     3481 ctcagtggtc acaccgcaaa tttcggcagc tggcgttgag catcttgata ctatcctcca
     3541 gccgtatatc aacgtcgaac caggccgggg caatcctcgc cgcgactttg aattacaaga
     3601 ggccaccatt actgattcgc gttacctgga ttaatgccca cctcgagcag atccggctgc
     3661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     3721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     3781 cggataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     4261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4501 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     4561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5161 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     5821 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
     5881 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     5941 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6001 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6061 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     6121 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     6181 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     6241 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     6301 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     6361 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     6421 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     6481 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     6541 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     6601 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     6661 cgggtcctca acgacaggag
//
