LOCUS       Exported                8679 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..285
                     /label=araBAD promoter region
                     /note="color: #cf00ff"
     primer_bind     219..248
                     /label=oDCE3179
                     /note="color: black; sequence: 
                     GCCATAGCATTTTTATCCATAAGATTAGCG"
     CDS             310..330
                     /label=Translation 310-330
                     /note="color: #993366; direction: RIGHT"
     CDS             328..384
                     /label=Translation 328-384
                     /note="color: #993366; direction: RIGHT"
     misc_feature    328..330
                     /label=Start
                     /note="color: #b1ff67"
     misc_feature    331..366
                     /label=8xHis2xQH Tag
                     /note="color: #ffef86"
     primer_bind     354..412
                     /label=CVP666
                     /note="color: black; sequence: 
                     TCAGCATCAACACgaaaatctgtattttcagatgGCTCTTAACACACCACAAATTACGC
                     "
     misc_feature    355..384
                     /label=30bp / 57.8
                     /note="color: #c6c9d1"
     misc_feature    367..384
                     /label=TEV Cleavage Site
                     /note="color: #ff00ff"
     CDS             385..1668
                     /label=inner membrane PqiA domain protein
                     /note="color: #ffef86; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             1637..4285
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     CDS             1637..4285
                     /label=Translation 1637-4285
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1694..1756
                     /label=TM helix (TOPCONS)
                     /note="color: #c6c9d1"
     misc_feature    1769..2047
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     primer_bind     1772..1801
                     /label=CVP676
                     /note="color: black; sequence: 
                     ggtaataccgtcaccatcgactttatgtcg"
     primer_bind     complement(1839..1861)
                     /label=CVP675
                     /note="color: black; sequence: tgttccgacttcaacgccctgat"
     misc_feature    1970..2023
                     /label=pore_loop1
                     /note="color: #85dae9"
     misc_feature    2111..2356
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     misc_feature    2312..2359
                     /label=Pore loop ring 2
                     /note="color: #85dae9"
     misc_feature    2471..2743
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    2672..2719
                     /label=pore_loop_3
                     /note="color: #85dae9"
     misc_feature    2807..3046
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     misc_feature    3002..3073
                     /label=pore_loop4
                     /note="color: #85dae9"
     misc_feature    3173..3535
                     /label=Ring_5
                     /note="color: #ff9ccd"
     misc_feature    3368..3418
                     /label=pore_loop_5
                     /note="color: #85dae9"
     misc_feature    3533..3826
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     primer_bind     complement(3719..3797)
                     /label=CVP745
                     /note="color: black; sequence: 
                     atgctctggagagcggggatgcctgtacggtcagaccactaccattcagctgaactttc
                     ggtgtgaccactgagaagcg"
     misc_feature    3719..3739
                     /label=59.9
                     /note="color: #85dae9"
     misc_feature    3737..3739
                     /label=pore_loop_6
                     /note="color: #85dae9"
     misc_feature    3740..3802
                     /label=Ring_5
                     /note="color: #ff9ccd"
     CDS             3740..3802
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     CDS             3740..3802
                     /label=Translation 3740-3802
                     /note="color: #993366; direction: RIGHT"
     primer_bind     3768..3826
                     /label=CVP746
                     /note="color: black; sequence: 
                     ccgtacaggcatccccgctctccagagcattaaagccgtatatcaacgtcgaaccaggc
                     "
     misc_feature    3803..3826
                     /label=""59.6""
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdco
                     /label=""59.6""/ApEinfo_revcolor=#85dae9/ApEinfo_fwdcolor=#
                     85dae9
                     /note="color: #a6acb3"
     misc_feature    3803..3805
                     /label=ring_6_pore_loop
                     /note="color: #85dae9"
     misc_feature    3890..4147
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    4091..4159
                     /label=pore_loop_7
                     /note="color: #85dae9"
     primer_bind     complement(4266..4317)
                     /label=CVP667
                     /note="color: black; sequence: 
                     TTCAGCGTCTCTTTTCTGGAATTTGGTACttatttgggaagcgcagttcccc"
     misc_feature    4286..4288
                     /label=STOP
                     /note="color: #a300ff"
     CDS             4286..4288
                     /label=Translation 4286-4288
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4289..4317
                     /label=29 bp /59.4
                     /note="color: #d59687"
     CDS             4289..4302
                     /label=Translation 4289-4302
                     /note="color: #993366; direction: RIGHT"
     primer_bind     4337..4361
                     /label=oDCE3221
                     /note="color: black; sequence: GGTCCTGGTCTGAGGAATGGTTGTA"
     primer_bind     complement(4362..4382)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     primer_bind     complement(4406..4435)
                     /label=CVBPl060
                     /note="color: black; sequence: 
                     CAGACCGCTTCTGCGTTCTGATTTAATCTG"
     terminator      4449..4606
                     /label=rrnB transcription terminator
                     /note="color: #c6c9d1"
     misc_feature    4585..4609
                     /label=BE2467
                     /note="color: #85dae9"
     CDS             4885..5745
                     /label=Ampicillin ORF
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    5429
                     /label=SeqResult T->C
                     /note="color: #d59687"
     rep_origin      6277..6958
                     /label=ColE1 origin
                     /note="color: #b7e6d7"
     misc_feature    complement(7002..7143)
                     /label=Fragment from pL452
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(7003..7012)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_recomb     7037..7070
                     /label=LoxP
                     /note="color: #d59687"
     gene            complement(7037..7070)
                     /label=loxP
                     /note="color: #f58a5e; direction: LEFT"
     primer_bind     complement(7118..7127)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_feature    7481
                     /label=SeqResC->A
                     /note="color: #d59687"
     misc_feature    7484..7486
                     /label=SeqResATC->GAG
                     /note="color: #d59687"
     misc_feature    7488..7692
                     /label=SeqRes 205bp insert
                     /note="color: #d59687"
     CDS             complement(7784..8671)
                     /label=araC
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
       61 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
      121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
      181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
      241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
      301 ttttttgggc tagccgaagg gtgaattatg catcaccatc accaccatca ccatcagcat
      361 caacacgaaa atctgtattt tcagatggct cttaacacac cacaaattac gccgacaaaa
      421 aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
      481 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
      541 cgctgtcagg caaaaattcg tgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
      601 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
      661 ctgttaggca ttcgtattga cgccaacgtt atgcaaggca tctggcaaat gaccaaacag
      721 ggcgatgcga taacggggtc gatggtcttt ttctgcgtta ttggtgcccc gctcattctg
      781 gtgacctcca tagcttattt atggtttggt aaccgactgg gaatgaattt acgtccggta
      841 ctgctgatgc ttgagcgact taaagagtgg gtaatgctcg atatctacct ggtcggcatt
      901 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggctggtgt cggcttgttc
      961 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaagaa
     1021 ctgtgggagc gattttatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc
     1081 tgtcttgggt gccattttac cggctatcca gatcagcgtg gtcgctgccc gcgttgccat
     1141 atcccgctac gcctgcgtcg ccgtcatagt ctgcaaaaat gctgggcggc gctgttagcg
     1201 tcaatcgttt tattgttacc tgccaacctg ttgcctattt ctatcattta tctgaacgga
     1261 ggacggcagg aagatacaat tctttccgga attatgtcgc tggcaagtag caacattgcg
     1321 gttgcaggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
     1381 ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg
     1441 ttactgcgga tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct
     1501 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
     1561 ttttatttcg gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc
     1621 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
     1681 aaataaacgc cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgccag
     1741 ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc
     1801 ggcggatggt attgttccag gccgtacgcc tgttcgttat cagggcgttg aagtcggaac
     1861 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
     1921 gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctggtga cgccaaaagc
     1981 atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat
     2041 gccgggtaaa ggtaaagagc aggatcactt tgtcgcactc gatacccaac cgaaatatcg
     2101 gctggacaat ggcgatctga tgatccacct gcaagccccc gatctcggtt cgctgaacag
     2161 cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa
     2221 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
     2281 gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca gtatcagtgg
     2341 cgcgaaggtg aaactggaaa gcctggcggc actggttaac ggtgcgattg ccttcgattc
     2401 accagaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc
     2461 ccacagccag cgtggcgtaa taataaaact ggaactgccg agtggggccg gattaaccgc
     2521 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
     2581 aaatcctggt ggtaaagtca caggggaaat gaccgttgat cccagcgtcg ttaccctgct
     2641 tcgtgaaaat acccgcatcg aattacgcaa cccgaaatta tcccttagcg atgctaatct
     2701 cagcgccctg ctgaccggca aaaccttcga gctggtgccc ggcgatggcg agccacgcaa
     2761 agagttcgtt gttgtgccag gcgaaaaagc actgctgcat gaacctgatg ttctgacgct
     2821 gaccctgacc gcgccggaaa gttacggtat tgatgcgggt cagccgctca ttcttcacgg
     2881 cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt
     2941 cgccatcgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg tcgtcaacag
     3001 ccgtgtcgat gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga
     3061 atggatcaat ggcgggatac gtattctgcc gggcgataaa ggtgagatga aagccagcta
     3121 tccactgtat gccaatttgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac
     3181 aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctgta
     3241 ccgtaaattt gaagttggtg aagtgattac cgtgcgtccg cgagctaacg cgtttgatat
     3301 cgatctgcat attaagccgg agtatcgcaa ccttctgacc agcaatagcg tgttctgggc
     3361 agaaggcggg gcgaaagttc agctgaatgg tagtggtctg accgtacagg catccccgct
     3421 ctccagagca ttaaagggag ccattagctt cgacaacctc agcggtgcca gcgccagtca
     3481 gcgtaaaggc gataagcgta ttctgtatgc ttccgaaaca gcggcccgtg cggttggcgg
     3541 gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta
     3601 tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcac gcaatgaagt
     3661 acaggcaaag gcggtacttt acccggaata tgtccagacc tttgctcgcg gtggtacgcg
     3721 cttctcagtg gtcacaccga aagttcagct gaatggtagt ggtctgaccg tacaggcatc
     3781 cccgctctcc agagcattaa agccgtatat caacgtcgaa ccaggccggg gcaatcctcg
     3841 ccgcgacttt gaattacaag aggccaccat tactgattcg cgttacctgg atggcttaag
     3901 cattattgtt gaagcgccgg aagccggttc gttaggcatc ggtacgcctg tgctgttccg
     3961 tggtctggaa gtcggtacgg ttacaggaat gacgctgggg acattgtcag atcgcgtgat
     4021 gattgcgatg cgcatcagta aacgctatca acacctggtg cgtaacaatt ccgtcttctg
     4081 gttggcatcg ggttacagtc tggactttgg tctgacgggc ggcgtagtga aaaccggcac
     4141 ctttaaccag tttatccgtg gcggcatcgc cttcgccacg cctccgggta cgccactggc
     4201 accgaaagcc caggaaggca aacacttcct gttgcaggaa agtgaaccga aagagtggcg
     4261 tgaatgggga actgcgcttc ccaaataagt accaaattcc agaaaagaga cgctgaaaag
     4321 cgtctttttt cgttttggtc ctggtctgag gaatggttgt aagcttggct gttttggcgg
     4381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     4441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     4501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     4561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     4621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     4681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     4741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     4801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5101 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5401 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5761 tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg
     5821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6001 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6061 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6121 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     6241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc tcagcagagc
     6481 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6961 tttacggttc ctggcctttt gctggccttt tgctcacatg ttagcttggc tggacgtaaa
     7021 ctcctcttca gacctaataa cttcgtatag catacattat acgaagttat attaagggtt
     7081 attgaatatg atcggaattg ggctgcagga attcgatagc ttggctgcag gtcgacctcg
     7141 aggacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7201 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7261 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7321 gcatagggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7381 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7441 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag aaggagatgg cgcccaacag
     7501 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     7561 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     7621 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctgct catgtttgac
     7681 agcttatcat cgatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7741 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7801 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7861 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7921 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     7981 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     8041 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     8101 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     8161 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     8221 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     8281 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     8341 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8401 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8461 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8521 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8581 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     8641 gatcattttg cgcttcagcc atacttttca tactcccgc
//
