LOCUS       Exported                8685 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..285
                     /label=araBAD promoter region
                     /note="color: #cf00ff"
     primer_bind     219..248
                     /label=oDCE3179
                     /note="color: black; sequence: 
                     GCCATAGCATTTTTATCCATAAGATTAGCG"
     CDS             310..330
                     /label=Translation 310-330
                     /note="color: #993366; direction: RIGHT"
     CDS             328..384
                     /label=Translation 328-384
                     /note="color: #993366; direction: RIGHT"
     misc_feature    328..330
                     /label=Start
                     /note="color: #b1ff67"
     misc_feature    331..366
                     /label=8xHis2xQH Tag
                     /note="color: #ffef86"
     misc_feature    355..384
                     /label=30bp / 57.8
                     /note="color: #c6c9d1"
     misc_feature    367..384
                     /label=TEV Cleavage Site
                     /note="color: #ff00ff"
     CDS             385..1668
                     /label=inner membrane PqiA domain protein
                     /note="color: #ffef86; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             1637..4291
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     CDS             1637..4291
                     /label=Translation 1637-4291
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1694..1756
                     /label=TM helix (TOPCONS)
                     /note="color: #c6c9d1"
     misc_feature    1769..2047
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    1970..2023
                     /label=pore_loop1
                     /note="color: #85dae9"
     misc_feature    2111..2356
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     primer_bind     2206..2227
                     /label=CVP640
                     /note="color: black; sequence: cgactatgccatcaatcccaac"
     misc_feature    2312..2359
                     /label=Pore loop ring 2
                     /note="color: #85dae9"
     misc_feature    2471..2743
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    2672..2719
                     /label=pore_loop_3
                     /note="color: #85dae9"
     misc_feature    2807..3046
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     primer_bind     2807..2825
                     /label=CVP641
                     /note="color: black; sequence: gatgttctgacgctgaccc"
     misc_feature    3002..3073
                     /label=pore_loop4
                     /note="color: #85dae9"
     misc_feature    3173..3535
                     /label=Ring_5
                     /note="color: #ff9ccd"
     misc_feature    3368..3418
                     /label=pore_loop_5
                     /note="color: #85dae9"
     misc_feature    3533..3832
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     primer_bind     complement(3713..3771)
                     /label=CVP756
                     /note="color: black; sequence: 
                     tcaacgccatccagccccaccttcacatcgacacggctgaccactgagaagcgcgtacc
                     "
     misc_feature    3713..3733
                     /label=59.9
                     /note="color: #faac61"
     primer_bind     3730..3832
                     /label=CVP755
                     /note="color: black; sequence: 
                     ggtcagccgtgtcgatgtgaaggtggggctggatggcgttgagtttctcggtgccagcg
                     cctcagaatggatcaatggcccgtatatcaacgtcgaaccaggc"
     CDS             3734..3808
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     misc_feature    3734..3808
                     /label=pore_loop4
                     /note="color: #85dae9"
     CDS             3734..3808
                     /label=Translation 3734-3808
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3734..3781
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     misc_feature    3809..3832
                     /label=59.6
                     /note="color: #f8d3a9"
     misc_feature    3809..3811
                     /label=pore_loop_6
                     /note="color: #85dae9"
     misc_feature    3896..4153
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    4097..4165
                     /label=pore_loop_7
                     /note="color: #85dae9"
     primer_bind     complement(4272..4323)
                     /label=CVP667
                     /note="color: black; sequence: 
                     TTCAGCGTCTCTTTTCTGGAATTTGGTACttatttgggaagcgcagttcccc"
     misc_feature    4292..4294
                     /label=STOP
                     /note="color: #a300ff"
     CDS             4292..4294
                     /label=Translation 4292-4294
                     /note="color: #993366; direction: RIGHT"
     CDS             4295..4308
                     /label=Translation 4295-4308
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4296..4323
                     /label=29 bp /59.4
                     /note="color: #d59687"
     primer_bind     4343..4367
                     /label=oDCE3221
                     /note="color: black; sequence: GGTCCTGGTCTGAGGAATGGTTGTA"
     primer_bind     complement(4368..4388)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     primer_bind     complement(4412..4441)
                     /label=CVBPl060
                     /note="color: black; sequence: 
                     CAGACCGCTTCTGCGTTCTGATTTAATCTG"
     terminator      4455..4612
                     /label=rrnB transcription terminator
                     /note="color: #c6c9d1"
     misc_feature    4591..4615
                     /label=BE2467
                     /note="color: #85dae9"
     CDS             4891..5751
                     /label=Ampicillin ORF
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    5435
                     /label=SeqResult T->C
                     /note="color: #d59687"
     rep_origin      6283..6964
                     /label=ColE1 origin
                     /note="color: #b7e6d7"
     misc_feature    complement(7008..7149)
                     /label=Fragment from pL452
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(7009..7018)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_recomb     7043..7076
                     /label=LoxP
                     /note="color: #d59687"
     gene            complement(7043..7076)
                     /label=loxP
                     /note="color: #f58a5e; direction: LEFT"
     primer_bind     complement(7124..7133)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_feature    7487
                     /label=SeqResC->A
                     /note="color: #d59687"
     misc_feature    7490..7492
                     /label=SeqResATC->GAG
                     /note="color: #d59687"
     misc_feature    7494..7698
                     /label=SeqRes 205bp insert
                     /note="color: #d59687"
     CDS             complement(7790..8677)
                     /label=araC
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
       61 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
      121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
      181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
      241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
      301 ttttttgggc tagccgaagg gtgaattatg catcaccatc accaccatca ccatcagcat
      361 caacacgaaa atctgtattt tcagatggct cttaacacac cacaaattac gccgacaaaa
      421 aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
      481 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
      541 cgctgtcagg caaaaattcg tgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
      601 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
      661 ctgttaggca ttcgtattga cgccaacgtt atgcaaggca tctggcaaat gaccaaacag
      721 ggcgatgcga taacggggtc gatggtcttt ttctgcgtta ttggtgcccc gctcattctg
      781 gtgacctcca tagcttattt atggtttggt aaccgactgg gaatgaattt acgtccggta
      841 ctgctgatgc ttgagcgact taaagagtgg gtaatgctcg atatctacct ggtcggcatt
      901 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggctggtgt cggcttgttc
      961 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaagaa
     1021 ctgtgggagc gattttatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc
     1081 tgtcttgggt gccattttac cggctatcca gatcagcgtg gtcgctgccc gcgttgccat
     1141 atcccgctac gcctgcgtcg ccgtcatagt ctgcaaaaat gctgggcggc gctgttagcg
     1201 tcaatcgttt tattgttacc tgccaacctg ttgcctattt ctatcattta tctgaacgga
     1261 ggacggcagg aagatacaat tctttccgga attatgtcgc tggcaagtag caacattgcg
     1321 gttgcaggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
     1381 ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg
     1441 ttactgcgga tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct
     1501 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
     1561 ttttatttcg gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc
     1621 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
     1681 aaataaacgc cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgccag
     1741 ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc
     1801 ggcggatggt attgttccag gccgtacgcc tgttcgttat cagggcgttg aagtcggaac
     1861 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
     1921 gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctggtga cgccaaaagc
     1981 atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat
     2041 gccgggtaaa ggtaaagagc aggatcactt tgtcgcactc gatacccaac cgaaatatcg
     2101 gctggacaat ggcgatctga tgatccacct gcaagccccc gatctcggtt cgctgaacag
     2161 cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa
     2221 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
     2281 gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca gtatcagtgg
     2341 cgcgaaggtg aaactggaaa gcctggcggc actggttaac ggtgcgattg ccttcgattc
     2401 accagaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc
     2461 ccacagccag cgtggcgtaa taataaaact ggaactgccg agtggggccg gattaaccgc
     2521 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
     2581 aaatcctggt ggtaaagtca caggggaaat gaccgttgat cccagcgtcg ttaccctgct
     2641 tcgtgaaaat acccgcatcg aattacgcaa cccgaaatta tcccttagcg atgctaatct
     2701 cagcgccctg ctgaccggca aaaccttcga gctggtgccc ggcgatggcg agccacgcaa
     2761 agagttcgtt gttgtgccag gcgaaaaagc actgctgcat gaacctgatg ttctgacgct
     2821 gaccctgacc gcgccggaaa gttacggtat tgatgcgggt cagccgctca ttcttcacgg
     2881 cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt
     2941 cgccatcgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg tcgtcaacag
     3001 ccgtgtcgat gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga
     3061 atggatcaat ggcgggatac gtattctgcc gggcgataaa ggtgagatga aagccagcta
     3121 tccactgtat gccaatttgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac
     3181 aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctgta
     3241 ccgtaaattt gaagttggtg aagtgattac cgtgcgtccg cgagctaacg cgtttgatat
     3301 cgatctgcat attaagccgg agtatcgcaa ccttctgacc agcaatagcg tgttctgggc
     3361 agaaggcggg gcgaaagttc agctgaatgg tagtggtctg accgtacagg catccccgct
     3421 ctccagagca ttaaagggag ccattagctt cgacaacctc agcggtgcca gcgccagtca
     3481 gcgtaaaggc gataagcgta ttctgtatgc ttccgaaaca gcggcccgtg cggttggcgg
     3541 gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta
     3601 tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcac gcaatgaagt
     3661 acaggcaaag gcggtacttt acccggaata tgtccagacc tttgctcgcg gtggtacgcg
     3721 cttctcagtg gtcagccgtg tcgatgtgaa ggtggggctg gatggcgttg agtttctcgg
     3781 tgccagcgcc tcagaatgga tcaatggccc gtatatcaac gtcgaaccag gccggggcaa
     3841 tcctcgccgc gactttgaat tacaagaggc caccattact gattcgcgtt acctggatgg
     3901 cttaagcatt attgttgaag cgccggaagc cggttcgtta ggcatcggta cgcctgtgct
     3961 gttccgtggt ctggaagtcg gtacggttac aggaatgacg ctggggacat tgtcagatcg
     4021 cgtgatgatt gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta acaattccgt
     4081 cttctggttg gcatcgggtt acagtctgga ctttggtctg acgggcggcg tagtgaaaac
     4141 cggcaccttt aaccagttta tccgtggcgg catcgccttc gccacgcctc cgggtacgcc
     4201 actggcaccg aaagcccagg aaggcaaaca cttcctgttg caggaaagtg aaccgaaaga
     4261 gtggcgtgaa tggggaactg cgcttcccaa ataagtacca aattccagaa aagagacgct
     4321 gaaaagcgtc ttttttcgtt ttggtcctgg tctgaggaat ggttgtaagc ttggctgttt
     4381 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     4441 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     4501 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     4561 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     4621 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     4681 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     4741 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     4801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4861 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4921 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4981 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5041 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5101 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5161 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5221 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5281 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5341 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5401 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     5461 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5521 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5581 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5641 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5701 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5761 caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc
     5821 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6001 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6121 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     6241 tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggctcag
     6481 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6541 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6601 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6661 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6721 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6781 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6841 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6901 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6961 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttag cttggctgga
     7021 cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga agttatatta
     7081 agggttattg aatatgatcg gaattgggct gcaggaattc gatagcttgg ctgcaggtcg
     7141 acctcgagga catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7261 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7321 cacaccgcat agggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7381 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7441 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagaagg agatggcgcc
     7501 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     7561 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     7621 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg
     7681 tttgacagct tatcatcgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     7741 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     7801 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     7861 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     7921 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     7981 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     8041 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     8101 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     8161 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     8221 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     8281 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     8341 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     8401 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     8461 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     8521 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     8581 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     8641 gcaggggatc attttgcgct tcagccatac ttttcatact cccgc
//
