LOCUS       Exported                8682 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..285
                     /label=araBAD promoter region
                     /note="color: #cf00ff"
     primer_bind     219..248
                     /label=oDCE3179
                     /note="color: black; sequence: 
                     GCCATAGCATTTTTATCCATAAGATTAGCG"
     CDS             310..330
                     /label=Translation 310-330
                     /note="color: #993366; direction: RIGHT"
     CDS             328..384
                     /label=Translation 328-384
                     /note="color: #993366; direction: RIGHT"
     misc_feature    328..330
                     /label=Start
                     /note="color: #b1ff67"
     misc_feature    331..366
                     /label=8xHis2xQH Tag
                     /note="color: #ffef86"
     misc_feature    355..384
                     /label=30bp / 57.8
                     /note="color: #c6c9d1"
     misc_feature    367..384
                     /label=TEV Cleavage Site
                     /note="color: #ff00ff"
     CDS             385..1668
                     /label=inner membrane PqiA domain protein
                     /note="color: #ffef86; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             385..1668
                     /label=Translation 385-1668
                     /note="color: #993366; direction: RIGHT"
     CDS             1637..4288
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     CDS             1637..4288
                     /label=Translation 1637-4288
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1694..1756
                     /label=TM helix (TOPCONS)
                     /note="color: #c6c9d1"
     misc_feature    1769..2047
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     primer_bind     1772..1801
                     /label=CVP676
                     /note="color: black; sequence: 
                     ggtaataccgtcaccatcgactttatgtcg"
     primer_bind     complement(1839..1861)
                     /label=CVP675
                     /note="color: black; sequence: tgttccgacttcaacgccctgat"
     misc_feature    1970..2023
                     /label=pore_loop1
                     /note="color: #85dae9"
     misc_feature    2111..2356
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     misc_feature    2312..2359
                     /label=Pore loop ring 2
                     /note="color: #85dae9"
     misc_feature    2471..2743
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    2672..2719
                     /label=pore_loop_3
                     /note="color: #85dae9"
     misc_feature    2807..3046
                     /label=MCE domain (weak/partial hit, CDD)
                     /note="color: #c6c9d1"
     misc_feature    3002..3073
                     /label=pore_loop4
                     /note="color: #85dae9"
     misc_feature    3173..3535
                     /label=Ring_5
                     /note="color: #ff9ccd"
     misc_feature    3368..3418
                     /label=pore_loop_5
                     /note="color: #85dae9"
     misc_feature    3533..3829
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     primer_bind     complement(3713..3771)
                     /label=CVP758
                     /note="color: black; sequence: 
                     acgccgcccgtcagaccaaagtccagactgtaacccgagaccactgagaagcgcgtacc
                     "
     misc_feature    3713..3733
                     /label=59.9
                     /note="color: #85dae9"
     primer_bind     3731..3832
                     /label=CVP757
                     /note="color: black; sequence: 
                     gtctcgggttacagtctggactttggtctgacgggcggcgtagtgaaaaccggcacctt
                     taaccagtttatccgtggctatatcaacgtcgaaccaggccgg"
     CDS             3734..3808
                     /label=yebT
                     /note="color: #faac61; direction: RIGHT"
     misc_feature    3734..3808
                     /label=pore_loop_7
                     /note="color: #85dae9"
     CDS             3734..3808
                     /label=Translation 3734-3808
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3734..3793
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    3809..3832
                     /label=""59.6""
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdco
                     /label=""59.6""/ApEinfo_revcolor=#85dae9/ApEinfo_fwdcolor=#
                     85dae9
                     /note="color: #a6acb3"
     misc_feature    3893..4150
                     /label=MCE domain (CDD)
                     /note="color: #c6c9d1"
     misc_feature    4094..4162
                     /label=pore_loop_7
                     /note="color: #85dae9"
     primer_bind     complement(4269..4320)
                     /label=CVP667
                     /note="color: black; sequence: 
                     TTCAGCGTCTCTTTTCTGGAATTTGGTACttatttgggaagcgcagttcccc"
     misc_feature    4289..4291
                     /label=STOP
                     /note="color: #a300ff"
     CDS             4289..4291
                     /label=Translation 4289-4291
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4292..4320
                     /label=29 bp /59.4
                     /note="color: #d59687"
     CDS             4292..4305
                     /label=Translation 4292-4305
                     /note="color: #993366; direction: RIGHT"
     primer_bind     4340..4364
                     /label=oDCE3221
                     /note="color: black; sequence: GGTCCTGGTCTGAGGAATGGTTGTA"
     primer_bind     complement(4365..4385)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     primer_bind     complement(4409..4438)
                     /label=CVBPl060
                     /note="color: black; sequence: 
                     CAGACCGCTTCTGCGTTCTGATTTAATCTG"
     terminator      4452..4609
                     /label=rrnB transcription terminator
                     /note="color: #c6c9d1"
     misc_feature    4588..4612
                     /label=BE2467
                     /note="color: #85dae9"
     CDS             4888..5748
                     /label=Ampicillin ORF
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    5432
                     /label=SeqResult T->C
                     /note="color: #d59687"
     rep_origin      6280..6961
                     /label=ColE1 origin
                     /note="color: #b7e6d7"
     misc_feature    complement(7005..7146)
                     /label=Fragment from pL452
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(7006..7015)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_recomb     7040..7073
                     /label=LoxP
                     /note="color: #d59687"
     gene            complement(7040..7073)
                     /label=loxP
                     /note="color: #f58a5e; direction: LEFT"
     primer_bind     complement(7121..7130)
                     /label=oDCE3180
                     /note="color: black; sequence: ATCCGCCAAAACAGCCAAGCT"
     misc_feature    7484
                     /label=SeqResC->A
                     /note="color: #d59687"
     misc_feature    7487..7489
                     /label=SeqResATC->GAG
                     /note="color: #d59687"
     misc_feature    7491..7695
                     /label=SeqRes 205bp insert
                     /note="color: #d59687"
     CDS             complement(7787..8674)
                     /label=araC
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
       61 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
      121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
      181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
      241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
      301 ttttttgggc tagccgaagg gtgaattatg catcaccatc accaccatca ccatcagcat
      361 caacacgaaa atctgtattt tcagatggct cttaacacac cacaaattac gccgacaaaa
      421 aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
      481 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
      541 cgctgtcagg caaaaattcg tgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
      601 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
      661 ctgttaggca ttcgtattga cgccaacgtt atgcaaggca tctggcaaat gaccaaacag
      721 ggcgatgcga taacggggtc gatggtcttt ttctgcgtta ttggtgcccc gctcattctg
      781 gtgacctcca tagcttattt atggtttggt aaccgactgg gaatgaattt acgtccggta
      841 ctgctgatgc ttgagcgact taaagagtgg gtaatgctcg atatctacct ggtcggcatt
      901 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggctggtgt cggcttgttc
      961 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaagaa
     1021 ctgtgggagc gattttatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc
     1081 tgtcttgggt gccattttac cggctatcca gatcagcgtg gtcgctgccc gcgttgccat
     1141 atcccgctac gcctgcgtcg ccgtcatagt ctgcaaaaat gctgggcggc gctgttagcg
     1201 tcaatcgttt tattgttacc tgccaacctg ttgcctattt ctatcattta tctgaacgga
     1261 ggacggcagg aagatacaat tctttccgga attatgtcgc tggcaagtag caacattgcg
     1321 gttgcaggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
     1381 ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg
     1441 ttactgcgga tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct
     1501 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
     1561 ttttatttcg gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc
     1621 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
     1681 aaataaacgc cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgccag
     1741 ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc
     1801 ggcggatggt attgttccag gccgtacgcc tgttcgttat cagggcgttg aagtcggaac
     1861 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
     1921 gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctggtga cgccaaaagc
     1981 atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat
     2041 gccgggtaaa ggtaaagagc aggatcactt tgtcgcactc gatacccaac cgaaatatcg
     2101 gctggacaat ggcgatctga tgatccacct gcaagccccc gatctcggtt cgctgaacag
     2161 cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa
     2221 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
     2281 gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca gtatcagtgg
     2341 cgcgaaggtg aaactggaaa gcctggcggc actggttaac ggtgcgattg ccttcgattc
     2401 accagaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc
     2461 ccacagccag cgtggcgtaa taataaaact ggaactgccg agtggggccg gattaaccgc
     2521 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
     2581 aaatcctggt ggtaaagtca caggggaaat gaccgttgat cccagcgtcg ttaccctgct
     2641 tcgtgaaaat acccgcatcg aattacgcaa cccgaaatta tcccttagcg atgctaatct
     2701 cagcgccctg ctgaccggca aaaccttcga gctggtgccc ggcgatggcg agccacgcaa
     2761 agagttcgtt gttgtgccag gcgaaaaagc actgctgcat gaacctgatg ttctgacgct
     2821 gaccctgacc gcgccggaaa gttacggtat tgatgcgggt cagccgctca ttcttcacgg
     2881 cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt
     2941 cgccatcgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg tcgtcaacag
     3001 ccgtgtcgat gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga
     3061 atggatcaat ggcgggatac gtattctgcc gggcgataaa ggtgagatga aagccagcta
     3121 tccactgtat gccaatttgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac
     3181 aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctgta
     3241 ccgtaaattt gaagttggtg aagtgattac cgtgcgtccg cgagctaacg cgtttgatat
     3301 cgatctgcat attaagccgg agtatcgcaa ccttctgacc agcaatagcg tgttctgggc
     3361 agaaggcggg gcgaaagttc agctgaatgg tagtggtctg accgtacagg catccccgct
     3421 ctccagagca ttaaagggag ccattagctt cgacaacctc agcggtgcca gcgccagtca
     3481 gcgtaaaggc gataagcgta ttctgtatgc ttccgaaaca gcggcccgtg cggttggcgg
     3541 gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta
     3601 tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcac gcaatgaagt
     3661 acaggcaaag gcggtacttt acccggaata tgtccagacc tttgctcgcg gtggtacgcg
     3721 cttctcagtg gtctcgggtt acagtctgga ctttggtctg acgggcggcg tagtgaaaac
     3781 cggcaccttt aaccagttta tccgtggcta tatcaacgtc gaaccaggcc ggggcaatcc
     3841 tcgccgcgac tttgaattac aagaggccac cattactgat tcgcgttacc tggatggctt
     3901 aagcattatt gttgaagcgc cggaagccgg ttcgttaggc atcggtacgc ctgtgctgtt
     3961 ccgtggtctg gaagtcggta cggttacagg aatgacgctg gggacattgt cagatcgcgt
     4021 gatgattgcg atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtctt
     4081 ctggttggca tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg
     4141 cacctttaac cagtttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact
     4201 ggcaccgaaa gcccaggaag gcaaacactt cctgttgcag gaaagtgaac cgaaagagtg
     4261 gcgtgaatgg ggaactgcgc ttcccaaata agtaccaaat tccagaaaag agacgctgaa
     4321 aagcgtcttt tttcgttttg gtcctggtct gaggaatggt tgtaagcttg gctgttttgg
     4381 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     4441 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     4501 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     4561 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     4621 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     4681 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     4741 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     4801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4861 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4921 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4981 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5041 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5101 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5161 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5221 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5281 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5341 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5401 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     5461 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5521 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5581 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5641 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5701 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5761 gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc
     5821 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5881 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5941 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6001 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6061 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6121 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6181 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     6241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggctcagcag
     6481 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttagctt ggctggacgt
     7021 aaactcctct tcagacctaa taacttcgta tagcatacat tatacgaagt tatattaagg
     7081 gttattgaat atgatcggaa ttgggctgca ggaattcgat agcttggctg caggtcgacc
     7141 tcgaggacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7201 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7261 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7321 accgcatagg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     7381 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7441 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagaaggaga tggcgcccaa
     7501 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     7561 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     7621 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct gctcatgttt
     7681 gacagcttat catcgatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
     7741 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
     7801 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     7861 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     7921 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     7981 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     8041 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     8101 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     8161 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     8221 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     8281 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     8341 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     8401 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     8461 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     8521 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     8581 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     8641 ggggatcatt ttgcgcttca gccatacttt tcatactccc gc
//
