LOCUS       Exported                7070 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      340..1022
                     /label=ColE1 origin
     primer_bind     2131..2150
                     /label=T7
     CDS             2211..3494
                     /label=inner membrane PqiA domain protein
     CDS             2211..3494
                     /label=Translation 2211-3494
     CDS             2211..3494
                     /label=Translation 2211-3494
     CDS             3463..6090
                     /label=yebT
     CDS             3463..6090
                     /label=Translation 3463-6090
     misc_feature    3520..3582
                     /label=TM helix (TOPCONS)
     misc_feature    3595..3873
                     /label=MCE domain (CDD)
     misc_feature    3796..3849
                     /label=pore_loop1
     misc_feature    3937..4182
                     /label=MCE domain (weak/partial hit, CDD)
     misc_feature    4138..4185
                     /label=Pore loop ring 2
     misc_feature    4297..4569
                     /label=MCE domain (CDD)
     misc_feature    4498..4545
                     /label=pore_loop_3
     misc_feature    4633..4872
                     /label=MCE domain (weak/partial hit, CDD)
     misc_feature    4828..4899
                     /label=pore_loop4
     misc_feature    4999..5361
                     /label=Ring_5
     misc_feature    5194..5244
                     /label=pore_loop_5
     misc_feature    5359..5631
                     /label=MCE domain (CDD)
     primer_bind     complement(5545..5602)
                     /label=CVP743
     misc_feature    5545..5565
                     /label=59.9
     misc_feature    5563..5565
                     /label=pore_loop_6
     misc_feature    5566..5607
                     /label=pore_loop_3
     CDS             5566..5607
                     /label=Translation 5566-5607
     primer_bind     5573..5631
                     /label=CVP744
     misc_feature    5608..5631
                     /label=59.6
     misc_feature    5608..5610
                     /label=pore_loop_6
     misc_feature    5695..5952
                     /label=MCE domain (CDD)
     misc_feature    5896..5964
                     /label=pore_loop_7
     misc_feature    6091..6093
                     /label=STOP
     CDS             6091..6093
                     /label=Translation 6091-6093
     terminator      6173..6219
                     /label=T7 terminator
     primer_bind     complement(6252..6271)
                     /label=oDCE3649
     CDS             join(6653..7070,1..242)
                     /label=AmpR
ORIGIN
        1 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
       61 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
      241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
      301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
      361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
      601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1141 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     1201 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     1261 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     1321 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1381 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1441 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     1501 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     1561 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     1621 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     1681 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     1741 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     1801 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     1861 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     1921 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     1981 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     2041 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     2101 acgctgcccg agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac
     2161 ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacat atggctctta
     2221 acacaccaca aattacgccg acaaaaaaga taacagtgag ggcaatcggc gaggaactgc
     2281 cgcgtggtga ttaccaacgt tgcccgcaat gtgacatgct gtttagcctg cccgagataa
     2341 attctcatca aagtgcctat tgtccgcgct gtcaggcaaa aattcgtgac gggcgcgact
     2401 ggtcgctaac gcgcctggcg gcaatggcct tcaccatgct gttgcttatg ccgtttgcct
     2461 ggggcgaacc gctgttgcat atctggctgt taggcattcg tattgacgcc aacgttatgc
     2521 aaggcatctg gcaaatgacc aaacagggcg atgcgataac ggggtcgatg gtctttttct
     2581 gcgttattgg tgccccgctc attctggtga cctccatagc ttatttatgg tttggtaacc
     2641 gactgggaat gaatttacgt ccggtactgc tgatgcttga gcgacttaaa gagtgggtaa
     2701 tgctcgatat ctacctggtc ggcattggcg ttgcttctat aaaggtacag gattatgccc
     2761 atatccaggc tggtgtcggc ttgttctctt ttgtggcgtt ggtgatttta acgacggtga
     2821 cgttgtcaca tcttaatgtc gaagaactgt gggagcgatt ttatccgcag cgccccgcta
     2881 cgcgtaggga cgagaaactt cgtgtctgtc ttgggtgcca ttttaccggc tatccagatc
     2941 agcgtggtcg ctgcccgcgt tgccatatcc cgctacgcct gcgtcgccgt catagtctgc
     3001 aaaaatgctg ggcggcgctg ttagcgtcaa tcgttttatt gttacctgcc aacctgttgc
     3061 ctatttctat catttatctg aacggaggac ggcaggaaga tacaattctt tccggaatta
     3121 tgtcgctggc aagtagcaac attgcggttg caggaatcgt gtttatcgcc agtattctgg
     3181 taccgtttac taaagtgatc gtcatgttca ctttactgtt gagcattcat tttaaatgcc
     3241 agcaaggttt acgcacacgc attctgttac tgcggatggt gacctggatt ggtcgctggt
     3301 cgatgctcga cctgtttgtc atatctttaa ccatgtcgct gattaatcgc gatcagatcc
     3361 tcgcttttac tatgggaccg gctgcgtttt atttcggcgc agcggtaatt ttgactattc
     3421 ttgctgtgga atggctggac agccgcttac tttgggatgc acatgagtca ggaaacgccc
     3481 gcttcgacga ctgaagcgca gattaaaaat aaacgccgta tctcaccttt ctggctgctg
     3541 ccgttcatcg cgctaatgat tgccagttgg ctgatttggg acagttatca ggaccggggt
     3601 aataccgtca ccatcgactt tatgtcggcg gatggtattg ttccaggccg tacgcctgtt
     3661 cgttatcagg gcgttgaagt cggaacagtg caggatatca gcctcagcga cgatcttcgt
     3721 aagattgaag tcaaggtcag catcaagtcc gatatgaaag atgcgctgcg cgaagagact
     3781 cagttctggc tggtgacgcc aaaagcatcg ttggcaggtg tctccgggct ggacgccctc
     3841 gtcggtggga actatatcgg catgatgccg ggtaaaggta aagagcagga tcactttgtc
     3901 gcactcgata cccaaccgaa atatcggctg gacaatggcg atctgatgat ccacctgcaa
     3961 gcccccgatc tcggttcgct gaacagcggt tcattggtct atttccgcaa gatcccggtg
     4021 ggaaaagtct acgactatgc catcaatccc aacaagcaag gcgtggtgat tgatgtcctg
     4081 atcgagcggc gttttaccga tctggtgaaa aaaggtagcc gtttctggaa cgtttccggc
     4141 gttgatgcca acgtcagtat cagtggcgcg aaggtgaaac tggaaagcct ggcggcactg
     4201 gttaacggtg cgattgcctt cgattcacca gaagagtcga aacctgccga ggcggaagat
     4261 acctttggtc tgtatgaaga tctggcccac agccagcgtg gcgtaataat aaaactggaa
     4321 ctgccgagtg gggccggatt aaccgccgac tcgacgccgt taatgtatca ggggctggaa
     4381 gtcggacagc tgactaaact ggatttaaat cctggtggta aagtcacagg ggaaatgacc
     4441 gttgatccca gcgtcgttac cctgcttcgt gaaaataccc gcatcgaatt acgcaacccg
     4501 aaattatccc ttagcgatgc taatctcagc gccctgctga ccggcaaaac cttcgagctg
     4561 gtgcccggcg atggcgagcc acgcaaagag ttcgttgttg tgccaggcga aaaagcactg
     4621 ctgcatgaac ctgatgttct gacgctgacc ctgaccgcgc cggaaagtta cggtattgat
     4681 gcgggtcagc cgctcattct tcacggcgtg caggtaggcc aggttatcga tcgtaaactc
     4741 accagcaaag gcgtcacctt taccgtcgcc atcgagcctc agcatcgcga actggtaaaa
     4801 ggcgatagca aatttgtcgt caacagccgt gtcgatgtga aggtggggct ggatggcgtt
     4861 gagtttctcg gtgccagcgc ctcagaatgg atcaatggcg ggatacgtat tctgccgggc
     4921 gataaaggtg agatgaaagc cagctatcca ctgtatgcca atttggaaaa agcgctggag
     4981 aacagcctta gcgatttacc caccacaacc gtgagtttga gtgcagagac gctgccggat
     5041 gtgcaggcag gatcggtagt gctgtaccgt aaatttgaag ttggtgaagt gattaccgtg
     5101 cgtccgcgag ctaacgcgtt tgatatcgat ctgcatatta agccggagta tcgcaacctt
     5161 ctgaccagca atagcgtgtt ctgggcagaa ggcggggcga aagttcagct gaatggtagt
     5221 ggtctgaccg tacaggcatc cccgctctcc agagcattaa agggagccat tagcttcgac
     5281 aacctcagcg gtgccagcgc cagtcagcgt aaaggcgata agcgtattct gtatgcttcc
     5341 gaaacagcgg cccgtgcggt tggcgggcag attacgcttc acgctttcga tgccggaaaa
     5401 ctggcggtcg ggatgccaat tcgctatctc ggtattgata tcgggcaaat ccagacgctg
     5461 gatctgatta ccgcacgcaa tgaagtacag gcaaaggcgg tactttaccc ggaatatgtc
     5521 cagacctttg ctcgcggtgg tacgcgcttc tcagtggtca caccgaaatt atcccttagc
     5581 gatgctaatc tcagcgccct gctgaccccg tatatcaacg tcgaaccagg ccggggcaat
     5641 cctcgccgcg actttgaatt acaagaggcc accattactg attcgcgtta cctggatggc
     5701 ttaagcatta ttgttgaagc gccggaagcc ggttcgttag gcatcggtac gcctgtgctg
     5761 ttccgtggtc tggaagtcgg tacggttaca ggaatgacgc tggggacatt gtcagatcgc
     5821 gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa caattccgtc
     5881 ttctggttgg catcgggtta cagtctggac tttggtctga cgggcggcgt agtgaaaacc
     5941 ggcaccttta accagtttat ccgtggcggc atcgccttcg ccacgcctcc gggtacgcca
     6001 ctggcaccga aagcccagga aggcaaacac ttcctgttgc aggaaagtga accgaaagag
     6061 tggcgtgaat ggggaactgc gcttcccaaa taatgcccac ctcgagcaga tccggctgct
     6121 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     6181 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     6241 ggataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6301 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     6721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6961 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     7021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
//
