LOCUS       Exported                7091 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     62..81
                     /label=T7
     CDS             142..1425
                     /label=inner membrane PqiA domain protein
     CDS             142..1425
                     /label=Translation 142-1425
     CDS             142..1425
                     /label=Translation 142-1425
     CDS             1394..4042
                     /label=yebT
     CDS             1394..4042
                     /label=Translation 1394-4042
     misc_feature    1451..1513
                     /label=TM helix (TOPCONS)
     misc_feature    1526..1804
                     /label=MCE domain (CDD)
     misc_feature    1727..1780
                     /label=pore_loop1
     misc_feature    1868..2113
                     /label=MCE domain (weak/partial hit, CDD)
     misc_feature    2069..2116
                     /label=Pore loop ring 2
     misc_feature    2228..2500
                     /label=MCE domain (CDD)
     misc_feature    2429..2476
                     /label=pore_loop_3
     misc_feature    2564..2803
                     /label=MCE domain (weak/partial hit, CDD)
     primer_bind     2564..2582
                     /label=CVP641
     misc_feature    2759..2830
                     /label=pore_loop4
     misc_feature    2930..3292
                     /label=Ring_5
     misc_feature    3125..3175
                     /label=pore_loop_5
     misc_feature    3290..3583
                     /label=MCE domain (CDD)
     primer_bind     3338..3359
                     /label=oDCE3607
     primer_bind     complement(3476..3554)
                     /label=CVP745
     misc_feature    3476..3496
                     /label=59.9
     misc_feature    3494..3496
                     /label=pore_loop_6
     CDS             3497..3559
                     /label=yebT
     misc_feature    3497..3559
                     /label=Ring_5
     CDS             3497..3559
                     /label=Translation 3497-3559
     primer_bind     3525..3583
                     /label=CVP746
     misc_feature    3560..3583
                     /label=""59.6""
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdco
                     /label=""59.6""/ApEinfo_revcolor=#85dae9/ApEinfo_fwdcolor=#
                     85dae9
     misc_feature    3560..3562
                     /label=ring_6_pore_loop
     primer_bind     3593..3614
                     /label=oDCE3605
     misc_feature    3647..3904
                     /label=MCE domain (CDD)
     primer_bind     complement(3694..3715)
                     /label=oDCE3778
     misc_feature    3848..3916
                     /label=pore_loop_7
     primer_bind     3985..4006
                     /label=oDCE3559
     primer_bind     complement(4022..4081)
                     /label=CVP681
     misc_feature    4043..4045
                     /label=STOP
     CDS             4043..4045
                     /label=Translation 4043-4045
     terminator      4125..4171
                     /label=T7 terminator
     primer_bind     complement(4204..4223)
                     /label=oDCE3649
     CDS             4605..5264
                     /label=AmpR
     rep_origin      5362..6044
                     /label=ColE1 origin
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
      181 ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa
      241 tgtgacatgc tgtttagcct gcccgagata aattctcatc aaagtgccta ttgtccgcgc
      301 tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc
      361 ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac cgctgttgca tatctggctg
      421 ttaggcattc gtattgacgc caacgttatg caaggcatct ggcaaatgac caaacagggc
      481 gatgcgataa cggggtcgat ggtctttttc tgcgttattg gtgccccgct cattctggtg
      541 acctccatag cttatttatg gtttggtaac cgactgggaa tgaatttacg tccggtactg
      601 ctgatgcttg agcgacttaa agagtgggta atgctcgata tctacctggt cggcattggc
      661 gttgcttcta taaaggtaca ggattatgcc catatccagg ctggtgtcgg cttgttctct
      721 tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt cgaagaactg
      781 tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact tcgtgtctgt
      841 cttgggtgcc attttaccgg ctatccagat cagcgtggtc gctgcccgcg ttgccatatc
      901 ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct gggcggcgct gttagcgtca
      961 atcgttttat tgttacctgc caacctgttg cctatttcta tcatttatct gaacggagga
     1021 cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa cattgcggtt
     1081 gcaggaatcg tgtttatcgc cagtattctg gtaccgttta ctaaagtgat cgtcatgttc
     1141 actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg cattctgtta
     1201 ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt catatcttta
     1261 accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc ggctgcgttt
     1321 tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga cagccgctta
     1381 ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa
     1441 taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga ttgccagttg
     1501 gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact ttatgtcggc
     1561 ggatggtatt gttccaggcc gtacgcctgt tcgttatcag ggcgttgaag tcggaacagt
     1621 gcaggatatc agcctcagcg acgatcttcg taagattgaa gtcaaggtca gcatcaagtc
     1681 cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg ctggtgacgc caaaagcatc
     1741 gttggcaggt gtctccgggc tggacgccct cgtcggtggg aactatatcg gcatgatgcc
     1801 gggtaaaggt aaagagcagg atcactttgt cgcactcgat acccaaccga aatatcggct
     1861 ggacaatggc gatctgatga tccacctgca agcccccgat ctcggttcgc tgaacagcgg
     1921 ttcattggtc tatttccgca agatcccggt gggaaaagtc tacgactatg ccatcaatcc
     1981 caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg cgttttaccg atctggtgaa
     2041 aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc aacgtcagta tcagtggcgc
     2101 gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt gcgattgcct tcgattcacc
     2161 agaagagtcg aaacctgccg aggcggaaga tacctttggt ctgtatgaag atctggccca
     2221 cagccagcgt ggcgtaataa taaaactgga actgccgagt ggggccggat taaccgccga
     2281 ctcgacgccg ttaatgtatc aggggctgga agtcggacag ctgactaaac tggatttaaa
     2341 tcctggtggt aaagtcacag gggaaatgac cgttgatccc agcgtcgtta ccctgcttcg
     2401 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
     2461 cgccctgctg accggcaaaa ccttcgagct ggtgcccggc gatggcgagc cacgcaaaga
     2521 gttcgttgtt gtgccaggcg aaaaagcact gctgcatgaa cctgatgttc tgacgctgac
     2581 cctgaccgcg ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
     2641 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
     2701 catcgagcct cagcatcgcg aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
     2761 tgtcgatgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
     2821 gatcaatggc gggatacgta ttctgccggg cgataaaggt gagatgaaag ccagctatcc
     2881 actgtatgcc aatttggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
     2941 cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
     3001 taaatttgaa gttggtgaag tgattaccgt gcgtccgcga gctaacgcgt ttgatatcga
     3061 tctgcatatt aagccggagt atcgcaacct tctgaccagc aatagcgtgt tctgggcaga
     3121 aggcggggcg aaagttcagc tgaatggtag tggtctgacc gtacaggcat ccccgctctc
     3181 cagagcatta aagggagcca ttagcttcga caacctcagc ggtgccagcg ccagtcagcg
     3241 taaaggcgat aagcgtattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcgggca
     3301 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
     3361 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
     3421 ggcaaaggcg gtactttacc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
     3481 ctcagtggtc acaccgaaag ttcagctgaa tggtagtggt ctgaccgtac aggcatcccc
     3541 gctctccaga gcattaaagc cgtatatcaa cgtcgaacca ggccggggca atcctcgccg
     3601 cgactttgaa ttacaagagg ccaccattac tgattcgcgt tacctggatg gcttaagcat
     3661 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     3721 tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc gcgtgatgat
     3781 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     3841 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     3901 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     3961 gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     4021 atggggaact gcgcttccca aataatgccc acctcgagca gatccggctg ctaacaaagc
     4081 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4141 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggataatt
     4201 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4261 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4621 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     4681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4921 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     4981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6181 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     6241 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     6301 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6481 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     6541 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     6601 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     6661 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     6721 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     6781 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     6841 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     6901 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     6961 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     7021 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     7081 aacgacagga g
//
