LOCUS       Exported                7094 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     62..81
                     /label=T7
     primer_bind     111..169
                     /label=CVP679
     CDS             142..1393
                     /label=inner membrane PqiA domain protein
     CDS             142..1393
                     /label=Translation 142-1393
     CDS             142..1393
                     /label=Translation 142-1393
     primer_bind     634..654
                     /label=CVP672
     primer_bind     1328..1349
                     /label=CVP673
     CDS             1390..1398
                     /label=Translation 1390-1398
     CDS             1394..4045
                     /label=yebT
     CDS             1394..4045
                     /label=Translation 1394-4045
     CDS             1394..1425
                     /label=inner membrane PqiA domain protein
     CDS             1396..1425
                     /label=Translation 1396-1425
     misc_feature    1451..1513
                     /label=TM helix (TOPCONS)
     misc_feature    1526..1804
                     /label=MCE domain (CDD)
     primer_bind     1529..1558
                     /label=CVP676
     primer_bind     complement(1596..1618)
                     /label=CVP675
     primer_bind     complement(1707..1731)
                     /label=CVP727
     misc_feature    1727..1780
                     /label=pore_loop1
     primer_bind     1780..1804
                     /label=CVP728
     misc_feature    1868..2113
                     /label=MCE domain (weak/partial hit, CDD)
     primer_bind     1963..1984
                     /label=CVP640
     misc_feature    2069..2116
                     /label=Pore loop ring 2
     misc_feature    2228..2500
                     /label=MCE domain (CDD)
     misc_feature    2429..2476
                     /label=pore_loop_3
     misc_feature    2564..2803
                     /label=MCE domain (weak/partial hit, CDD)
     primer_bind     2564..2582
                     /label=CVP641
     misc_feature    2759..2830
                     /label=pore_loop4
     misc_feature    2930..3292
                     /label=Ring_5
     misc_feature    3125..3175
                     /label=pore_loop_5
     misc_feature    3290..3586
                     /label=MCE domain (CDD)
     primer_bind     complement(3470..3571)
                     /label=CVP754
     misc_feature    3470..3490
                     /label=21 - 59.7
     misc_feature    3491..3565
                     /label=Pore loop ring 2
     CDS             3491..3565
                     /label=Translation 3491-3565
     primer_bind     3531..3589
                     /label=CVP753
     misc_feature    3566..3589
                     /label=60.5
     misc_feature    3650..3907
                     /label=MCE domain (CDD)
     misc_feature    3851..3919
                     /label=pore_loop_7
     primer_bind     complement(4025..4084)
                     /label=CVP681
     misc_feature    4046..4048
                     /label=STOP
     CDS             4046..4048
                     /label=Translation 4046-4048
     terminator      4128..4174
                     /label=T7 terminator
     primer_bind     complement(4207..4226)
                     /label=oDCE3649
     CDS             4608..5267
                     /label=AmpR
     rep_origin      5365..6047
                     /label=ColE1 origin
     primer_bind     complement(5755..5813)
                     /label=CVP347
     primer_bind     5938..5961
                     /label=CVP669
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
      181 ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa
      241 tgtgacatgc tgtttagcct gcccgagata aattctcatc aaagtgccta ttgtccgcgc
      301 tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc
      361 ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac cgctgttgca tatctggctg
      421 ttaggcattc gtattgacgc caacgttatg caaggcatct ggcaaatgac caaacagggc
      481 gatgcgataa cggggtcgat ggtctttttc tgcgttattg gtgccccgct cattctggtg
      541 acctccatag cttatttatg gtttggtaac cgactgggaa tgaatttacg tccggtactg
      601 ctgatgcttg agcgacttaa agagtgggta atgctcgata tctacctggt cggcattggc
      661 gttgcttcta taaaggtaca ggattatgcc catatccagg ctggtgtcgg cttgttctct
      721 tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt cgaagaactg
      781 tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact tcgtgtctgt
      841 cttgggtgcc attttaccgg ctatccagat cagcgtggtc gctgcccgcg ttgccatatc
      901 ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct gggcggcgct gttagcgtca
      961 atcgttttat tgttacctgc caacctgttg cctatttcta tcatttatct gaacggagga
     1021 cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa cattgcggtt
     1081 gcaggaatcg tgtttatcgc cagtattctg gtaccgttta ctaaagtgat cgtcatgttc
     1141 actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg cattctgtta
     1201 ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt catatcttta
     1261 accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc ggctgcgttt
     1321 tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga cagccgctta
     1381 ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa
     1441 taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga ttgccagttg
     1501 gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact ttatgtcggc
     1561 ggatggtatt gttccaggcc gtacgcctgt tcgttatcag ggcgttgaag tcggaacagt
     1621 gcaggatatc agcctcagcg acgatcttcg taagattgaa gtcaaggtca gcatcaagtc
     1681 cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg ctggtgacgc caaaagcatc
     1741 gttggcaggt gtctccgggc tggacgccct cgtcggtggg aactatatcg gcatgatgcc
     1801 gggtaaaggt aaagagcagg atcactttgt cgcactcgat acccaaccga aatatcggct
     1861 ggacaatggc gatctgatga tccacctgca agcccccgat ctcggttcgc tgaacagcgg
     1921 ttcattggtc tatttccgca agatcccggt gggaaaagtc tacgactatg ccatcaatcc
     1981 caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg cgttttaccg atctggtgaa
     2041 aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc aacgtcagta tcagtggcgc
     2101 gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt gcgattgcct tcgattcacc
     2161 agaagagtcg aaacctgccg aggcggaaga tacctttggt ctgtatgaag atctggccca
     2221 cagccagcgt ggcgtaataa taaaactgga actgccgagt ggggccggat taaccgccga
     2281 ctcgacgccg ttaatgtatc aggggctgga agtcggacag ctgactaaac tggatttaaa
     2341 tcctggtggt aaagtcacag gggaaatgac cgttgatccc agcgtcgtta ccctgcttcg
     2401 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
     2461 cgccctgctg accggcaaaa ccttcgagct ggtgcccggc gatggcgagc cacgcaaaga
     2521 gttcgttgtt gtgccaggcg aaaaagcact gctgcatgaa cctgatgttc tgacgctgac
     2581 cctgaccgcg ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
     2641 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
     2701 catcgagcct cagcatcgcg aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
     2761 tgtcgatgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
     2821 gatcaatggc gggatacgta ttctgccggg cgataaaggt gagatgaaag ccagctatcc
     2881 actgtatgcc aatttggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
     2941 cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
     3001 taaatttgaa gttggtgaag tgattaccgt gcgtccgcga gctaacgcgt ttgatatcga
     3061 tctgcatatt aagccggagt atcgcaacct tctgaccagc aatagcgtgt tctgggcaga
     3121 aggcggggcg aaagttcagc tgaatggtag tggtctgacc gtacaggcat ccccgctctc
     3181 cagagcatta aagggagcca ttagcttcga caacctcagc ggtgccagcg ccagtcagcg
     3241 taaaggcgat aagcgtattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcgggca
     3301 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
     3361 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
     3421 ggcaaaggcg gtactttacc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
     3481 ctcagtggtc tccggcgttg atgccaacgt cagtatcagt ggcgcgaagg tgaaactgga
     3541 aagcctggcg gcactggtta acggttatat caacgtcgaa ccaggccggg gcaatcctcg
     3601 ccgcgacttt gaattacaag aggccaccat tactgattcg cgttacctgg atggcttaag
     3661 cattattgtt gaagcgccgg aagccggttc gttaggcatc ggtacgcctg tgctgttccg
     3721 tggtctggaa gtcggtacgg ttacaggaat gacgctgggg acattgtcag atcgcgtgat
     3781 gattgcgatg cgcatcagta aacgctatca acacctggtg cgtaacaatt ccgtcttctg
     3841 gttggcatcg ggttacagtc tggactttgg tctgacgggc ggcgtagtga aaaccggcac
     3901 ctttaaccag tttatccgtg gcggcatcgc cttcgccacg cctccgggta cgccactggc
     3961 accgaaagcc caggaaggca aacacttcct gttgcaggaa agtgaaccga aagagtggcg
     4021 tgaatgggga actgcgcttc ccaaataatg cccacctcga gcagatccgg ctgctaacaa
     4081 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     4141 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     4201 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4261 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4621 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     4681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4921 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     4981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5281 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5581 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6181 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     6241 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc
     6301 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     6361 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6421 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6481 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     6541 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     6601 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     6661 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     6721 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     6781 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     6841 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     6901 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     6961 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     7021 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     7081 ctcaacgaca ggag
//
