LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     222..243
                     /label=oDCE3561
     rep_origin      391..1073
                     /label=ColE1 origin
     primer_bind     837..858
                     /label=oDCE3562
     primer_bind     1450..1469
                     /label=oDCE3563
     primer_bind     complement(1623..1644)
                     /label=oDCE3648
     primer_bind     2038..2057
                     /label=oDCE3564
     primer_bind     2182..2201
                     /label=T7
     misc_feature    2262..3545
                     /label=YebS
     CDS             2262..3545
                     /label=Translation 2262-3545
     primer_bind     2709..2727
                     /label=oDCE3554
     misc_feature    2960
                     /label=T->C mutation relative to reference sequence
     misc_feature    2963
                     /label=T->A mutation relative to reference sequence
     misc_feature    3233
                     /label=A->C mutation relative to reference sequence
     primer_bind     3315..3335
                     /label=oDCE3555
     misc_feature    3514..6147
                     /label=YebT
     CDS             3514..6147
                     /label=Translation 3514-6147
     misc_feature    3747
                     /label=T->C mutation relative to reference sequence
     primer_bind     3811..3832
                     /label=oDCE3804
     primer_bind     3881..3898
                     /label=oDCE3802
     primer_bind     4924..4966
                     /label=oDCE3756
     primer_bind     complement(4924..4966)
                     /label=oDCE3757
     misc_feature    4942..4944
                     /label=I477N
     primer_bind     complement(4972..4996)
                     /label=oDCE3803
     primer_bind     complement(5042..5064)
                     /label=oDCE3806
     misc_feature    5175
                     /label=T->C mutation relative to reference sequence
     misc_feature    5307..5308
                     /label=AT->TC mutations relative to reference sequence
     misc_feature    5490
                     /label=T->C mutation relative to reference sequence
     primer_bind     5625..5644
                     /label=oDCE3805
     primer_bind     5695..5716
                     /label=oDCE3605
     misc_feature    5742
                     /label=T->C mutation relative to reference sequence
     primer_bind     complement(5796..5817)
                     /label=oDCE3778
     primer_bind     complement(5865..5886)
                     /label=oDCE3781
     primer_bind     complement(6212..6230)
                     /label=T7 Term
     terminator      6227..6273
                     /label=T7 terminator
     primer_bind     6676..6696
                     /label=oDCE3560
     CDS             join(6707..7073,1..293)
                     /label=AmpR
ORIGIN
        1 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
       61 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
      181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
      241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
      301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
      361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
      421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      601 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1081 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1141 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     1201 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     1261 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
     1321 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     1381 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1441 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1501 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     1561 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     1621 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     1681 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     1741 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     1801 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     1861 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     1921 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     1981 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     2041 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     2101 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     2161 gagatctcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa cggtttccct
     2221 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatggctctt aacacaccac
     2281 aaattacgcc gacaaaaaag ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg
     2341 attaccaacg ttgcccgcaa tgtgacatgc tgtttagcct gcccgagata aattctcatc
     2401 aaagtgccta ttgtccgcgc tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa
     2461 cgcgcctggc ggcaatggcc ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac
     2521 cgctgttgca tatctggctg ttaggcattc gtattgacgc caacgttatg caaggcatct
     2581 ggcaaatgac caaacagggc gatgcgataa cggggtcgat ggtctttttc tgcgttattg
     2641 gtgccccgct cattctggtg acctccatag cttatttatg gtttggtaac cgactgggaa
     2701 tgaatttacg tccggtactg ctgatgcttg agcgacttaa agagtgggta atgctcgata
     2761 tctacctggt cggcattggc gttgcttcta taaaggtaca ggattatgcc catatccagg
     2821 ctggtgtcgg cttgttctct tttgtggcgt tggtgatttt aacgacggtg acgttgtcac
     2881 atcttaatgt cgaagaactg tgggagcgat tttatccgca gcgccccgct acgcgtaggg
     2941 acgagaaact tcgtgtctgc ctagggtgcc attttaccgg ctatccagat cagcgtggtc
     3001 gctgcccgcg ttgccatatc ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct
     3061 gggcggcgct gttagcgtca atcgttttat tgttacctgc caacctgttg cctatttcta
     3121 tcatttatct gaacggagga cggcaggaag atacaattct ttccggaatt atgtcgctgg
     3181 caagtagcaa cattgcggtt gcaggaatcg tgtttatcgc cagtattctg gtcccgttta
     3241 ctaaagtgat cgtcatgttc actttactgt tgagcattca ttttaaatgc cagcaaggtt
     3301 tacgcacacg cattctgtta ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg
     3361 acctgtttgt catatcttta accatgtcgc tgattaatcg cgatcagatc ctcgctttta
     3421 ctatgggacc ggctgcgttt tatttcggcg cagcggtaat tttgactatt cttgctgtgg
     3481 aatggctgga cagccgctta ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg
     3541 actgaagcgc agattaaaaa taaacgccgt atctcacctt tctggctgct gccgttcatc
     3601 gcgctaatga ttgccagttg gctgatttgg gacagttatc aggaccgggg taataccgtc
     3661 accatcgact ttatgtcggc ggatggtatt gttccaggcc gtacgcctgt tcgttatcag
     3721 ggcgttgaag tcggaacagt gcaggacatc agcctcagcg acgatcttcg taagattgaa
     3781 gtcaaggtca gcatcaagtc cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg
     3841 ctggtgacgc caaaagcatc gttggcaggt gtctccgggc tggacgccct cgtcggtggg
     3901 aactatatcg gcatgatgcc gggtaaaggt aaagagcagg atcactttgt cgcactcgat
     3961 acccaaccga aatatcggct ggacaatggc gatctgatga tccacctgca agcccccgat
     4021 ctcggttcgc tgaacagcgg ttcattggtc tatttccgca agatcccggt gggaaaagtc
     4081 tacgactatg ccatcaatcc caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg
     4141 cgttttaccg atctggtgaa aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc
     4201 aacgtcagta tcagtggcgc gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt
     4261 gcgattgcct tcgattcacc agaagagtcg aaacctgccg aggcggaaga tacctttggt
     4321 ctgtatgaag atctggccca cagccagcgt ggcgtaataa taaaactgga actgccgagt
     4381 ggggccggat taaccgccga ctcgacgccg ttaatgtatc aggggctgga agtcggacag
     4441 ctgactaaac tggatttaaa tcctggtggt aaagtcacag gggaaatgac cgttgatccc
     4501 agcgtcgtta ccctgcttcg tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc
     4561 cttagcgatg ctaatctcag cgccctgctg accggcaaaa ccttcgagct ggtgcccggc
     4621 gatggcgagc cacgcaaaga gttcgttgtt gtgccaggcg aaaaagcact gctgcatgaa
     4681 cctgatgttc tgacgctgac cctgaccgcg ccggaaagtt acggtattga tgcgggtcag
     4741 ccgctcattc ttcacggcgt gcaggtaggc caggttatcg atcgtaaact caccagcaaa
     4801 ggcgtcacct ttaccgtcgc catcgagcct cagcatcgcg aactggtaaa aggcgatagc
     4861 aaatttgtcg tcaacagccg tgtcgatgtg aaggtggggc tggatggcgt tgagtttctc
     4921 ggtgccagcg cctcagaatg gaataatggc gggatacgta ttctgccggg cgataaaggt
     4981 gagatgaaag ccagctatcc actgtatgcc aatttggaaa aagcgctgga gaacagcctt
     5041 agcgatttac ccaccacaac cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca
     5101 ggatcggtag tgctgtaccg taaatttgaa gttggtgaag tgattaccgt gcgtccgcga
     5161 gctaacgcgt ttgacatcga tctgcatatt aagccggagt atcgcaacct tctgaccagc
     5221 aatagcgtgt tctgggcaga aggcggggcg aaagttcagc tgaatggtag tggtctgacc
     5281 gtacaggcat ccccgctctc cagagctcta aagggagcca ttagcttcga caacctcagc
     5341 ggtgccagcg ccagtcagcg taaaggcgat aagcgtattc tgtatgcttc cgaaacagcg
     5401 gcccgtgcgg ttggcgggca gattacgctt cacgctttcg atgccggaaa actggcggtc
     5461 gggatgccaa ttcgctatct cggtattgac atcgggcaaa tccagacgct ggatctgatt
     5521 accgcacgca atgaagtaca ggcaaaggcg gtactttacc cggaatatgt ccagaccttt
     5581 gctcgcggtg gtacgcgctt ctcagtggtc acaccgcaaa tttcggcagc tggcgttgag
     5641 catcttgata ctatcctcca gccgtatatc aacgtcgaac caggccgggg caatcctcgc
     5701 cgcgactttg aattacaaga ggccaccatt actgattcgc ggtacctgga tggcttaagc
     5761 attattgttg aagcgccgga agccggttcg ttaggcatcg gtacgcctgt gctgttccgt
     5821 ggtctggaag tcggtacggt tacaggaatg acgctgggga cattgtcaga tcgcgtgatg
     5881 attgcgatgc gcatcagtaa acgctatcaa cacctggtgc gtaacaattc cgtcttctgg
     5941 ttggcatcgg gttacagtct ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc
     6001 tttaaccagt ttatccgtgg cggcatcgcc ttcgccacgc ctccgggtac gccactggca
     6061 ccgaaagccc aggaaggcaa acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt
     6121 gaatggggaa ctgcgcttcc caaataatgc ccacctcgag cagatccggc tgctaacaaa
     6181 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     6241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa
     6301 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6361 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6721 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     6781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7021 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgc
//
