LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     237..258
                     /label=oDCE3561
     rep_origin      406..1088
                     /label=ColE1 origin
     primer_bind     852..873
                     /label=oDCE3562
     primer_bind     1465..1484
                     /label=oDCE3563
     primer_bind     complement(1638..1659)
                     /label=oDCE3648
     primer_bind     2053..2072
                     /label=oDCE3564
     primer_bind     2197..2216
                     /label=T7
     misc_feature    2277..3560
                     /label=YebS
     CDS             2277..3560
                     /label=Translation 2277-3560
     primer_bind     2724..2742
                     /label=oDCE3554
     misc_feature    2975
                     /label=T->C mutation relative to reference sequence
     misc_feature    2978
                     /label=T->A mutation relative to reference sequence
     misc_feature    3248
                     /label=A->C mutation relative to reference sequence
     primer_bind     3330..3350
                     /label=oDCE3555
     misc_feature    3529..6162
                     /label=YebT
     CDS             3529..6162
                     /label=Translation 3529-6162
     misc_feature    3762
                     /label=T->C mutation relative to reference sequence
     primer_bind     3826..3847
                     /label=oDCE3804
     primer_bind     3896..3913
                     /label=oDCE3802
     misc_feature    3936
                     /label=G->C mutation relative to reference sequence
     misc_feature    3939
                     /label=T->G mutation relative to reference sequence
     misc_feature    4255
                     /label=C->T mutation relative to reference sequence
     misc_feature    4656
                     /label=G->A mutation relative to reference sequence
     primer_bind     4747..4765
                     /label=oDCE3780
     primer_bind     4804..4824
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     4892..4911
                     /label=oDCE3608
     primer_bind     5293..5334
                     /label=oDCE3758
     primer_bind     complement(5293..5334)
                     /label=oDCE3759
     misc_feature    5311..5313
                     /label=L595N
     primer_bind     complement(5811..5832)
                     /label=oDCE3778
     primer_bind     complement(5880..5901)
                     /label=oDCE3781
     primer_bind     complement(6227..6245)
                     /label=T7 Term
     terminator      6242..6288
                     /label=T7 terminator
     primer_bind     6691..6711
                     /label=oDCE3560
     CDS             join(6722..7073,1..308)
                     /label=AmpR
ORIGIN
        1 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
       61 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      121 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      181 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      241 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      301 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      361 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      421 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      601 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
      661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1081 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1141 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1201 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     1261 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     1321 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     1381 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1501 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     1561 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     1621 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     1681 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     1741 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     1801 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     1861 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     1921 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     1981 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     2041 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     2101 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     2161 gacccaacgc tgcccgagat ctcgatcccg cgaaattaat acgactcact atagggagac
     2221 cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat atacatatgg
     2281 ctcttaacac accacaaatt acgccgacaa aaaagataac agtgagggca atcggcgagg
     2341 aactgccgcg tggtgattac caacgttgcc cgcaatgtga catgctgttt agcctgcccg
     2401 agataaattc tcatcaaagt gcctattgtc cgcgctgtca ggcaaaaatt cgtgacgggc
     2461 gcgactggtc gctaacgcgc ctggcggcaa tggccttcac catgctgttg cttatgccgt
     2521 ttgcctgggg cgaaccgctg ttgcatatct ggctgttagg cattcgtatt gacgccaacg
     2581 ttatgcaagg catctggcaa atgaccaaac agggcgatgc gataacgggg tcgatggtct
     2641 ttttctgcgt tattggtgcc ccgctcattc tggtgacctc catagcttat ttatggtttg
     2701 gtaaccgact gggaatgaat ttacgtccgg tactgctgat gcttgagcga cttaaagagt
     2761 gggtaatgct cgatatctac ctggtcggca ttggcgttgc ttctataaag gtacaggatt
     2821 atgcccatat ccaggctggt gtcggcttgt tctcttttgt ggcgttggtg attttaacga
     2881 cggtgacgtt gtcacatctt aatgtcgaag aactgtggga gcgattttat ccgcagcgcc
     2941 ccgctacgcg tagggacgag aaacttcgtg tctgcctagg gtgccatttt accggctatc
     3001 cagatcagcg tggtcgctgc ccgcgttgcc atatcccgct acgcctgcgt cgccgtcata
     3061 gtctgcaaaa atgctgggcg gcgctgttag cgtcaatcgt tttattgtta cctgccaacc
     3121 tgttgcctat ttctatcatt tatctgaacg gaggacggca ggaagataca attctttccg
     3181 gaattatgtc gctggcaagt agcaacattg cggttgcagg aatcgtgttt atcgccagta
     3241 ttctggtccc gtttactaaa gtgatcgtca tgttcacttt actgttgagc attcatttta
     3301 aatgccagca aggtttacgc acacgcattc tgttactgcg gatggtgacc tggattggtc
     3361 gctggtcgat gctcgacctg tttgtcatat ctttaaccat gtcgctgatt aatcgcgatc
     3421 agatcctcgc ttttactatg ggaccggctg cgttttattt cggcgcagcg gtaattttga
     3481 ctattcttgc tgtggaatgg ctggacagcc gcttactttg ggatgcacat gagtcaggaa
     3541 acgcccgctt cgacgactga agcgcagatt aaaaataaac gccgtatctc acctttctgg
     3601 ctgctgccgt tcatcgcgct aatgattgcc agttggctga tttgggacag ttatcaggac
     3661 cggggtaata ccgtcaccat cgactttatg tcggcggatg gtattgttcc aggccgtacg
     3721 cctgttcgtt atcagggcgt tgaagtcgga acagtgcagg acatcagcct cagcgacgat
     3781 cttcgtaaga ttgaagtcaa ggtcagcatc aagtccgata tgaaagatgc gctgcgcgaa
     3841 gagactcagt tctggctggt gacgccaaaa gcatcgttgg caggtgtctc cgggctggac
     3901 gccctcgtcg gtgggaacta tatcggcatg atgcccggga aaggtaaaga gcaggatcac
     3961 tttgtcgcac tcgataccca accgaaatat cggctggaca atggcgatct gatgatccac
     4021 ctgcaagccc ccgatctcgg ttcgctgaac agcggttcat tggtctattt ccgcaagatc
     4081 ccggtgggaa aagtctacga ctatgccatc aatcccaaca agcaaggcgt ggtgattgat
     4141 gtcctgatcg agcggcgttt taccgatctg gtgaaaaaag gtagccgttt ctggaacgtt
     4201 tccggcgttg atgccaacgt cagtatcagt ggcgcgaagg tgaaactgga aagcttggcg
     4261 gcactggtta acggtgcgat tgccttcgat tcaccagaag agtcgaaacc tgccgaggcg
     4321 gaagatacct ttggtctgta tgaagatctg gcccacagcc agcgtggcgt aataataaaa
     4381 ctggaactgc cgagtggggc cggattaacc gccgactcga cgccgttaat gtatcagggg
     4441 ctggaagtcg gacagctgac taaactggat ttaaatcctg gtggtaaagt cacaggggaa
     4501 atgaccgttg atcccagcgt cgttaccctg cttcgtgaaa atacccgcat cgaattacgc
     4561 aacccgaaat tatcccttag cgatgctaat ctcagcgccc tgctgaccgg caaaaccttc
     4621 gagctggtgc ccggcgatgg cgagccacgc aaagaattcg ttgttgtgcc aggcgaaaaa
     4681 gcactgctgc atgaacctga tgttctgacg ctgaccctga ccgcgccgga aagttacggt
     4741 attgatgcgg gtcagccgct cattcttcac ggcgtgcagg taggccaggt tatcgatcgt
     4801 aaactcacca gcaaaggcgt cacctttacc gtcgccatcg agcctcagca tcgcgaactg
     4861 gtaaaaggcg atagcaaatt tgtcgtcaac agccgtgtcg atgtgaaggt ggggctggat
     4921 ggcgttgagt ttctcggtgc cagcgcctca gaatggatca atggcgggat acgtattctg
     4981 ccgggcgata aaggtgagat gaaagccagc tatccactgt atgccaattt ggaaaaagcg
     5041 ctggagaaca gccttagcga tttacccacc acaaccgtga gtttgagtgc agagacgctg
     5101 ccggatgtgc aggcaggatc ggtagtgctg taccgtaaat ttgaagttgg tgaagtgatt
     5161 accgtgcgtc cgcgagctaa cgcgtttgat atcgatctgc atattaagcc ggagtatcgc
     5221 aaccttctga ccagcaatag cgtgttctgg gcagaaggcg gggcgaaagt tcagctgaat
     5281 ggtagtggtc tgaccgtaca ggcatccccg aattccagag cattaaaggg agccattagc
     5341 ttcgacaacc tcagcggtgc cagcgccagt cagcgtaaag gcgataagcg tattctgtat
     5401 gcttccgaaa cagcggcccg tgcggttggc gggcagatta cgcttcacgc tttcgatgcc
     5461 ggaaaactgg cggtcgggat gccaattcgc tatctcggta ttgatatcgg gcaaatccag
     5521 acgctggatc tgattaccgc acgcaatgaa gtacaggcaa aggcggtact ttacccggaa
     5581 tatgtccaga cctttgctcg cggtggtacg cgcttctcag tggtcacacc gcaaatttcg
     5641 gcagctggcg ttgagcatct tgatactatc ctccagccgt atatcaacgt cgaaccaggc
     5701 cggggcaatc ctcgccgcga ctttgaatta caagaggcca ccattactga ttcgcgttac
     5761 ctggatggct taagcattat tgttgaagcg ccggaagccg gttcgttagg catcggtacg
     5821 cctgtgctgt tccgtggtct ggaagtcggt acggttacag gaatgacgct ggggacattg
     5881 tcagatcgcg tgatgattgc gatgcgcatc agtaaacgct atcaacacct ggtgcgtaac
     5941 aattccgtct tctggttggc atcgggttac agtctggact ttggtctgac gggcggcgta
     6001 gtgaaaaccg gcacctttaa ccagtttatc cgtggcggca tcgccttcgc cacgcctccg
     6061 ggtacgccac tggcaccgaa agcccaggaa ggcaaacact tcctgttgca ggaaagtgaa
     6121 ccgaaagagt ggcgtgaatg gggaactgcg cttcccaaat aatgcccacc tcgagcagat
     6181 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     6241 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     6301 actatatccg gataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     6361 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     6781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caa
//
