LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     230..251
                     /label=oDCE3561
     rep_origin      399..1081
                     /label=ColE1 origin
     primer_bind     845..866
                     /label=oDCE3562
     primer_bind     1458..1477
                     /label=oDCE3563
     primer_bind     complement(1631..1652)
                     /label=oDCE3648
     primer_bind     2046..2065
                     /label=oDCE3564
     primer_bind     2190..2209
                     /label=T7
     misc_feature    2270..3553
                     /label=YebS
     CDS             2270..3553
                     /label=Translation 2270-3553
     primer_bind     2717..2735
                     /label=oDCE3554
     misc_feature    2968
                     /label=T->C mutation relative to reference sequence
     misc_feature    2971
                     /label=T->A mutation relative to reference sequence
     misc_feature    3241
                     /label=A->C mutation relative to reference sequence
     primer_bind     3323..3343
                     /label=oDCE3555
     misc_feature    3522..6155
                     /label=YebT
     CDS             3522..6155
                     /label=Translation 3522-6155
     misc_feature    3755
                     /label=T->C mutation relative to reference sequence
     primer_bind     3819..3840
                     /label=oDCE3804
     primer_bind     3889..3906
                     /label=oDCE3802
     misc_feature    3929
                     /label=G->C mutation relative to reference sequence
     misc_feature    3932
                     /label=T->G mutation relative to reference sequence
     misc_feature    4248
                     /label=C->T mutation relative to reference sequence
     misc_feature    4649
                     /label=G->A mutation relative to reference sequence
     primer_bind     4797..4817
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     5295..5338
                     /label=oDCE3760
     primer_bind     complement(5295..5338)
                     /label=oDCE3761
     misc_feature    5313..5315
                     /label=A598N
     primer_bind     complement(5873..5894)
                     /label=oDCE3781
     primer_bind     complement(6220..6238)
                     /label=T7 Term
     terminator      6235..6281
                     /label=T7 terminator
     primer_bind     6684..6704
                     /label=oDCE3560
     CDS             join(6715..7073,1..301)
                     /label=AmpR
ORIGIN
        1 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
       61 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      121 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      181 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      241 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      301 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      421 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      601 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
      661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1141 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1201 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     1261 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     1321 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     1381 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1441 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1501 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     1561 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     1621 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     1681 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     1741 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     1801 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     1861 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     1921 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     1981 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     2041 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     2101 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa
     2161 cgctgcccga gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
     2221 gtttccctct agaaataatt ttgtttaact ttaagaagga gatatacata tggctcttaa
     2281 cacaccacaa attacgccga caaaaaagat aacagtgagg gcaatcggcg aggaactgcc
     2341 gcgtggtgat taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa
     2401 ttctcatcaa agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg
     2461 gtcgctaacg cgcctggcgg caatggcctt caccatgctg ttgcttatgc cgtttgcctg
     2521 gggcgaaccg ctgttgcata tctggctgtt aggcattcgt attgacgcca acgttatgca
     2581 aggcatctgg caaatgacca aacagggcga tgcgataacg gggtcgatgg tctttttctg
     2641 cgttattggt gccccgctca ttctggtgac ctccatagct tatttatggt ttggtaaccg
     2701 actgggaatg aatttacgtc cggtactgct gatgcttgag cgacttaaag agtgggtaat
     2761 gctcgatatc tacctggtcg gcattggcgt tgcttctata aaggtacagg attatgccca
     2821 tatccaggct ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac
     2881 gttgtcacat cttaatgtcg aagaactgtg ggagcgattt tatccgcagc gccccgctac
     2941 gcgtagggac gagaaacttc gtgtctgcct agggtgccat tttaccggct atccagatca
     3001 gcgtggtcgc tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca
     3061 aaaatgctgg gcggcgctgt tagcgtcaat cgttttattg ttacctgcca acctgttgcc
     3121 tatttctatc atttatctga acggaggacg gcaggaagat acaattcttt ccggaattat
     3181 gtcgctggca agtagcaaca ttgcggttgc aggaatcgtg tttatcgcca gtattctggt
     3241 cccgtttact aaagtgatcg tcatgttcac tttactgttg agcattcatt ttaaatgcca
     3301 gcaaggttta cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc
     3361 gatgctcgac ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct
     3421 cgcttttact atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct
     3481 tgctgtggaa tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg
     3541 cttcgacgac tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc
     3601 cgttcatcgc gctaatgatt gccagttggc tgatttggga cagttatcag gaccggggta
     3661 ataccgtcac catcgacttt atgtcggcgg atggtattgt tccaggccgt acgcctgttc
     3721 gttatcaggg cgttgaagtc ggaacagtgc aggacatcag cctcagcgac gatcttcgta
     3781 agattgaagt caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc
     3841 agttctggct ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg
     3901 tcggtgggaa ctatatcggc atgatgcccg ggaaaggtaa agagcaggat cactttgtcg
     3961 cactcgatac ccaaccgaaa tatcggctgg acaatggcga tctgatgatc cacctgcaag
     4021 cccccgatct cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg
     4081 gaaaagtcta cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga
     4141 tcgagcggcg ttttaccgat ctggtgaaaa aaggtagccg tttctggaac gtttccggcg
     4201 ttgatgccaa cgtcagtatc agtggcgcga aggtgaaact ggaaagcttg gcggcactgg
     4261 ttaacggtgc gattgccttc gattcaccag aagagtcgaa acctgccgag gcggaagata
     4321 cctttggtct gtatgaagat ctggcccaca gccagcgtgg cgtaataata aaactggaac
     4381 tgccgagtgg ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag
     4441 tcggacagct gactaaactg gatttaaatc ctggtggtaa agtcacaggg gaaatgaccg
     4501 ttgatcccag cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga
     4561 aattatccct tagcgatgct aatctcagcg ccctgctgac cggcaaaacc ttcgagctgg
     4621 tgcccggcga tggcgagcca cgcaaagaat tcgttgttgt gccaggcgaa aaagcactgc
     4681 tgcatgaacc tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac ggtattgatg
     4741 cgggtcagcc gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca
     4801 ccagcaaagg cgtcaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag
     4861 gcgatagcaa atttgtcgtc aacagccgtg tcgatgtgaa ggtggggctg gatggcgttg
     4921 agtttctcgg tgccagcgcc tcagaatgga tcaatggcgg gatacgtatt ctgccgggcg
     4981 ataaaggtga gatgaaagcc agctatccac tgtatgccaa tttggaaaaa gcgctggaga
     5041 acagccttag cgatttaccc accacaaccg tgagtttgag tgcagagacg ctgccggatg
     5101 tgcaggcagg atcggtagtg ctgtaccgta aatttgaagt tggtgaagtg attaccgtgc
     5161 gtccgcgagc taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc
     5221 tgaccagcaa tagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg
     5281 gtctgaccgt acaggcatcc ccgctctcca gaaacttaaa gggagccatt agcttcgaca
     5341 acctcagcgg tgccagcgcc agtcagcgta aaggcgataa gcgtattctg tatgcttccg
     5401 aaacagcggc ccgtgcggtt ggcgggcaga ttacgcttca cgctttcgat gccggaaaac
     5461 tggcggtcgg gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg
     5521 atctgattac cgcacgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc
     5581 agacctttgc tcgcggtggt acgcgcttct cagtggtcac accgcaaatt tcggcagctg
     5641 gcgttgagca tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca
     5701 atcctcgccg cgactttgaa ttacaagagg ccaccattac tgattcgcgt tacctggatg
     5761 gcttaagcat tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc
     5821 tgttccgtgg tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc
     5881 gcgtgatgat tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg
     5941 tcttctggtt ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa
     6001 ccggcacctt taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc
     6061 cactggcacc gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag
     6121 agtggcgtga atggggaact gcgcttccca aataatgccc acctcgagca gatccggctg
     6181 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     6241 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     6301 ccggataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6361 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6721 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     6781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7021 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aac
//
