LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     220..241
                     /label=oDCE3561
     rep_origin      389..1071
                     /label=ColE1 origin
     primer_bind     835..856
                     /label=oDCE3562
     primer_bind     1448..1467
                     /label=oDCE3563
     primer_bind     complement(1621..1642)
                     /label=oDCE3648
     primer_bind     2036..2055
                     /label=oDCE3564
     primer_bind     2180..2199
                     /label=T7
     misc_feature    2260..3543
                     /label=YebS
     CDS             2260..3543
                     /label=Translation 2260-3543
     primer_bind     2707..2725
                     /label=oDCE3554
     misc_feature    2958
                     /label=T->C mutation relative to reference sequence
     misc_feature    2961
                     /label=T->A mutation relative to reference sequence
     misc_feature    3231
                     /label=A->C mutation relative to reference sequence
     primer_bind     3313..3333
                     /label=oDCE3555
     misc_feature    3512..6145
                     /label=YebT
     CDS             3512..6145
                     /label=Translation 3512-6145
     misc_feature    3745
                     /label=T->C mutation relative to reference sequence
     primer_bind     3809..3830
                     /label=oDCE3804
     primer_bind     3879..3896
                     /label=oDCE3802
     misc_feature    3919
                     /label=G->C mutation relative to reference sequence
     misc_feature    3922
                     /label=T->G mutation relative to reference sequence
     misc_feature    4238
                     /label=C->T mutation relative to reference sequence
     misc_feature    4639
                     /label=G->A mutation relative to reference sequence
     primer_bind     4787..4807
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     5286..5328
                     /label=oDCE3762
     primer_bind     complement(5286..5328)
                     /label=oDCE3763
     misc_feature    5306..5308
                     /label=L599N
     primer_bind     complement(5863..5884)
                     /label=oDCE3781
     primer_bind     complement(6210..6228)
                     /label=T7 Term
     terminator      6225..6271
                     /label=T7 terminator
     primer_bind     6674..6694
                     /label=oDCE3560
     CDS             join(6705..7073,1..291)
                     /label=AmpR
ORIGIN
        1 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
       61 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
      241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
      301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      601 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     1261 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     1321 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     1381 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1441 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1501 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     1561 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     1621 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     1681 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     1741 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     1801 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     1861 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     1921 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     1981 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     2041 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     2101 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     2161 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
     2221 agaaataatt ttgtttaact ttaagaagga gatatacata tggctcttaa cacaccacaa
     2281 attacgccga caaaaaagat aacagtgagg gcaatcggcg aggaactgcc gcgtggtgat
     2341 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
     2401 agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg
     2461 cgcctggcgg caatggcctt caccatgctg ttgcttatgc cgtttgcctg gggcgaaccg
     2521 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgttatgca aggcatctgg
     2581 caaatgacca aacagggcga tgcgataacg gggtcgatgg tctttttctg cgttattggt
     2641 gccccgctca ttctggtgac ctccatagct tatttatggt ttggtaaccg actgggaatg
     2701 aatttacgtc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctcgatatc
     2761 tacctggtcg gcattggcgt tgcttctata aaggtacagg attatgccca tatccaggct
     2821 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
     2881 cttaatgtcg aagaactgtg ggagcgattt tatccgcagc gccccgctac gcgtagggac
     2941 gagaaacttc gtgtctgcct agggtgccat tttaccggct atccagatca gcgtggtcgc
     3001 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
     3061 gcggcgctgt tagcgtcaat cgttttattg ttacctgcca acctgttgcc tatttctatc
     3121 atttatctga acggaggacg gcaggaagat acaattcttt ccggaattat gtcgctggca
     3181 agtagcaaca ttgcggttgc aggaatcgtg tttatcgcca gtattctggt cccgtttact
     3241 aaagtgatcg tcatgttcac tttactgttg agcattcatt ttaaatgcca gcaaggttta
     3301 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     3361 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     3421 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     3481 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     3541 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     3601 gctaatgatt gccagttggc tgatttggga cagttatcag gaccggggta ataccgtcac
     3661 catcgacttt atgtcggcgg atggtattgt tccaggccgt acgcctgttc gttatcaggg
     3721 cgttgaagtc ggaacagtgc aggacatcag cctcagcgac gatcttcgta agattgaagt
     3781 caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc agttctggct
     3841 ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg tcggtgggaa
     3901 ctatatcggc atgatgcccg ggaaaggtaa agagcaggat cactttgtcg cactcgatac
     3961 ccaaccgaaa tatcggctgg acaatggcga tctgatgatc cacctgcaag cccccgatct
     4021 cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg gaaaagtcta
     4081 cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     4141 ttttaccgat ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     4201 cgtcagtatc agtggcgcga aggtgaaact ggaaagcttg gcggcactgg ttaacggtgc
     4261 gattgccttc gattcaccag aagagtcgaa acctgccgag gcggaagata cctttggtct
     4321 gtatgaagat ctggcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     4381 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     4441 gactaaactg gatttaaatc ctggtggtaa agtcacaggg gaaatgaccg ttgatcccag
     4501 cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga aattatccct
     4561 tagcgatgct aatctcagcg ccctgctgac cggcaaaacc ttcgagctgg tgcccggcga
     4621 tggcgagcca cgcaaagaat tcgttgttgt gccaggcgaa aaagcactgc tgcatgaacc
     4681 tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac ggtattgatg cgggtcagcc
     4741 gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca ccagcaaagg
     4801 cgtcaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     4861 atttgtcgtc aacagccgtg tcgatgtgaa ggtggggctg gatggcgttg agtttctcgg
     4921 tgccagcgcc tcagaatgga tcaatggcgg gatacgtatt ctgccgggcg ataaaggtga
     4981 gatgaaagcc agctatccac tgtatgccaa tttggaaaaa gcgctggaga acagccttag
     5041 cgatttaccc accacaaccg tgagtttgag tgcagagacg ctgccggatg tgcaggcagg
     5101 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtg attaccgtgc gtccgcgagc
     5161 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     5221 tagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg gtctgaccgt
     5281 acaggcatcc ccgctctcca gagcaaataa gggagccatt agcttcgaca acctcagcgg
     5341 tgccagcgcc agtcagcgta aaggcgataa gcgtattctg tatgcttccg aaacagcggc
     5401 ccgtgcggtt ggcgggcaga ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     5461 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
     5521 cgcacgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     5581 tcgcggtggt acgcgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     5641 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcgccg
     5701 cgactttgaa ttacaagagg ccaccattac tgattcgcgt tacctggatg gcttaagcat
     5761 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     5821 tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc gcgtgatgat
     5881 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     5941 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     6001 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     6061 gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     6121 atggggaact gcgcttccca aataatgccc acctcgagca gatccggctg ctaacaaagc
     6181 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     6241 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggataatt
     6301 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6361 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6421 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6481 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6541 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6601 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6661 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6721 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     6781 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6841 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6901 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6961 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7021 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgc
//
