LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3
                     /label=T->C mutation relative to reference sequence
     primer_bind     67..88
                     /label=oDCE3804
     primer_bind     137..154
                     /label=oDCE3802
     misc_feature    177
                     /label=G->C mutation relative to reference sequence
     misc_feature    180
                     /label=T->G mutation relative to reference sequence
     misc_feature    496
                     /label=C->T mutation relative to reference sequence
     misc_feature    897
                     /label=G->A mutation relative to reference sequence
     primer_bind     1045..1065
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     1882..1928
                     /label=oDCE3764
     primer_bind     complement(1882..1928)
                     /label=oDCE3765
     misc_feature    1900..1902
                     /label=L711N
     primer_bind     complement(2121..2142)
                     /label=oDCE3781
     primer_bind     complement(2468..2486)
                     /label=T7 Term
     terminator      2483..2529
                     /label=T7 terminator
     primer_bind     2932..2952
                     /label=oDCE3560
     CDS             2963..3622
                     /label=AmpR
     primer_bind     3551..3572
                     /label=oDCE3561
     rep_origin      3720..4402
                     /label=ColE1 origin
     primer_bind     4166..4187
                     /label=oDCE3562
     primer_bind     4779..4798
                     /label=oDCE3563
     primer_bind     complement(4952..4973)
                     /label=oDCE3648
     primer_bind     5367..5386
                     /label=oDCE3564
     primer_bind     5511..5530
                     /label=T7
     misc_feature    5591..6874
                     /label=YebS
     CDS             5591..6874
                     /label=Translation 5591-6874
     primer_bind     6038..6056
                     /label=oDCE3554
     misc_feature    6289
                     /label=T->C mutation relative to reference sequence
     misc_feature    6292
                     /label=T->A mutation relative to reference sequence
     misc_feature    6562
                     /label=A->C mutation relative to reference sequence
     primer_bind     6644..6664
                     /label=oDCE3555
     misc_feature    join(6843..7073,1..2403)
                     /label=YebT
     CDS             join(6843..7073,1..2403)
                     /label=Translation 6843-2403
ORIGIN
        1 gacatcagcc tcagcgacga tcttcgtaag attgaagtca aggtcagcat caagtccgat
       61 atgaaagatg cgctgcgcga agagactcag ttctggctgg tgacgccaaa agcatcgttg
      121 gcaggtgtct ccgggctgga cgccctcgtc ggtgggaact atatcggcat gatgcccggg
      181 aaaggtaaag agcaggatca ctttgtcgca ctcgataccc aaccgaaata tcggctggac
      241 aatggcgatc tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa cagcggttca
      301 ttggtctatt tccgcaagat cccggtggga aaagtctacg actatgccat caatcccaac
      361 aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa
      421 ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg tcagtatcag tggcgcgaag
      481 gtgaaactgg aaagcttggc ggcactggtt aacggtgcga ttgccttcga ttcaccagaa
      541 gagtcgaaac ctgccgaggc ggaagatacc tttggtctgt atgaagatct ggcccacagc
      601 cagcgtggcg taataataaa actggaactg ccgagtgggg ccggattaac cgccgactcg
      661 acgccgttaa tgtatcaggg gctggaagtc ggacagctga ctaaactgga tttaaatcct
      721 ggtggtaaag tcacagggga aatgaccgtt gatcccagcg tcgttaccct gcttcgtgaa
      781 aatacccgca tcgaattacg caacccgaaa ttatccctta gcgatgctaa tctcagcgcc
      841 ctgctgaccg gcaaaacctt cgagctggtg cccggcgatg gcgagccacg caaagaattc
      901 gttgttgtgc caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg
      961 accgcgccgg aaagttacgg tattgatgcg ggtcagccgc tcattcttca cggcgtgcag
     1021 gtaggccagg ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc
     1081 gagcctcagc atcgcgaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc
     1141 gatgtgaagg tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatc
     1201 aatggcggga tacgtattct gccgggcgat aaaggtgaga tgaaagccag ctatccactg
     1261 tatgccaatt tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg
     1321 agtttgagtg cagagacgct gccggatgtg caggcaggat cggtagtgct gtaccgtaaa
     1381 tttgaagttg gtgaagtgat taccgtgcgt ccgcgagcta acgcgtttga tatcgatctg
     1441 catattaagc cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc
     1501 ggggcgaaag ttcagctgaa tggtagtggt ctgaccgtac aggcatcccc gctctccaga
     1561 gcattaaagg gagccattag cttcgacaac ctcagcggtg ccagcgccag tcagcgtaaa
     1621 ggcgataagc gtattctgta tgcttccgaa acagcggccc gtgcggttgg cgggcagatt
     1681 acgcttcacg ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt
     1741 attgatatcg ggcaaatcca gacgctggat ctgattaccg cacgcaatga agtacaggca
     1801 aaggcggtac tttacccgga atatgtccag acctttgctc gcggtggtac gcgcttctca
     1861 gtggtcacac cgcaaatttc ggcagctggc gttgagcata atgatactat cctccagccg
     1921 tatatcaacg tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc
     1981 accattactg attcgcgtta cctggatggc ttaagcatta ttgttgaagc gccggaagcc
     2041 ggttcgttag gcatcggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca
     2101 ggaatgacgc tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc
     2161 tatcaacacc tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac
     2221 tttggtctga cgggcggcgt agtgaaaacc ggcaccttta accagtttat ccgtggcggc
     2281 atcgccttcg ccacgcctcc gggtacgcca ctggcaccga aagcccagga aggcaaacac
     2341 ttcctgttgc aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa
     2401 taatgcccac ctcgagcaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     2461 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     2521 ggttttttgc tgaaaggagg aactatatcc ggataattct tgaagacgaa agggcctcgt
     2581 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3001 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3061 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3121 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3181 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3241 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3301 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3361 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3421 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3481 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3541 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3601 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3661 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3721 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4501 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4561 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     4621 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     4681 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     4741 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4801 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     4861 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     4921 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     4981 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     5041 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     5101 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     5161 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     5221 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     5281 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     5341 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     5401 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     5461 gcacccgtgg ccaggaccca acgctgcccg agatctcgat cccgcgaaat taatacgact
     5521 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
     5581 agatatacat atggctctta acacaccaca aattacgccg acaaaaaaga taacagtgag
     5641 ggcaatcggc gaggaactgc cgcgtggtga ttaccaacgt tgcccgcaat gtgacatgct
     5701 gtttagcctg cccgagataa attctcatca aagtgcctat tgtccgcgct gtcaggcaaa
     5761 aattcgtgac gggcgcgact ggtcgctaac gcgcctggcg gcaatggcct tcaccatgct
     5821 gttgcttatg ccgtttgcct ggggcgaacc gctgttgcat atctggctgt taggcattcg
     5881 tattgacgcc aacgttatgc aaggcatctg gcaaatgacc aaacagggcg atgcgataac
     5941 ggggtcgatg gtctttttct gcgttattgg tgccccgctc attctggtga cctccatagc
     6001 ttatttatgg tttggtaacc gactgggaat gaatttacgt ccggtactgc tgatgcttga
     6061 gcgacttaaa gagtgggtaa tgctcgatat ctacctggtc ggcattggcg ttgcttctat
     6121 aaaggtacag gattatgccc atatccaggc tggtgtcggc ttgttctctt ttgtggcgtt
     6181 ggtgatttta acgacggtga cgttgtcaca tcttaatgtc gaagaactgt gggagcgatt
     6241 ttatccgcag cgccccgcta cgcgtaggga cgagaaactt cgtgtctgcc tagggtgcca
     6301 ttttaccggc tatccagatc agcgtggtcg ctgcccgcgt tgccatatcc cgctacgcct
     6361 gcgtcgccgt catagtctgc aaaaatgctg ggcggcgctg ttagcgtcaa tcgttttatt
     6421 gttacctgcc aacctgttgc ctatttctat catttatctg aacggaggac ggcaggaaga
     6481 tacaattctt tccggaatta tgtcgctggc aagtagcaac attgcggttg caggaatcgt
     6541 gtttatcgcc agtattctgg tcccgtttac taaagtgatc gtcatgttca ctttactgtt
     6601 gagcattcat tttaaatgcc agcaaggttt acgcacacgc attctgttac tgcggatggt
     6661 gacctggatt ggtcgctggt cgatgctcga cctgtttgtc atatctttaa ccatgtcgct
     6721 gattaatcgc gatcagatcc tcgcttttac tatgggaccg gctgcgtttt atttcggcgc
     6781 agcggtaatt ttgactattc ttgctgtgga atggctggac agccgcttac tttgggatgc
     6841 acatgagtca ggaaacgccc gcttcgacga ctgaagcgca gattaaaaat aaacgccgta
     6901 tctcaccttt ctggctgctg ccgttcatcg cgctaatgat tgccagttgg ctgatttggg
     6961 acagttatca ggaccggggt aataccgtca ccatcgactt tatgtcggcg gatggtattg
     7021 ttccaggccg tacgcctgtt cgttatcagg gcgttgaagt cggaacagtg cag
//
