LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    35
                     /label=T->C mutation relative to reference sequence
     primer_bind     99..120
                     /label=oDCE3804
     primer_bind     169..186
                     /label=oDCE3802
     misc_feature    209
                     /label=G->C mutation relative to reference sequence
     misc_feature    212
                     /label=T->G mutation relative to reference sequence
     misc_feature    528
                     /label=C->T mutation relative to reference sequence
     misc_feature    929
                     /label=G->A mutation relative to reference sequence
     primer_bind     1077..1097
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     complement(1193..1202)
                     /label=oDCE3767
     primer_bind     1919..1965
                     /label=oDCE3766
     primer_bind     complement(1919..1965)
                     /label=oDCE3767
     misc_feature    1941..1943
                     /label=I714N
     primer_bind     complement(2153..2174)
                     /label=oDCE3781
     primer_bind     complement(2500..2518)
                     /label=T7 Term
     terminator      2515..2561
                     /label=T7 terminator
     primer_bind     2964..2984
                     /label=oDCE3560
     CDS             2995..3654
                     /label=AmpR
     primer_bind     3583..3604
                     /label=oDCE3561
     rep_origin      3752..4434
                     /label=ColE1 origin
     primer_bind     4198..4219
                     /label=oDCE3562
     primer_bind     4811..4830
                     /label=oDCE3563
     primer_bind     complement(4984..5005)
                     /label=oDCE3648
     primer_bind     5399..5418
                     /label=oDCE3564
     primer_bind     5543..5562
                     /label=T7
     misc_feature    5623..6906
                     /label=YebS
     CDS             5623..6906
                     /label=Translation 5623-6906
     primer_bind     6070..6088
                     /label=oDCE3554
     misc_feature    6321
                     /label=T->C mutation relative to reference sequence
     misc_feature    6324
                     /label=T->A mutation relative to reference sequence
     misc_feature    6594
                     /label=A->C mutation relative to reference sequence
     primer_bind     6676..6696
                     /label=oDCE3555
     misc_feature    join(6875..7073,1..2435)
                     /label=YebT
     CDS             join(6875..7073,1..2435)
                     /label=Translation 6875-2435
ORIGIN
        1 gttatcaggg cgttgaagtc ggaacagtgc aggacatcag cctcagcgac gatcttcgta
       61 agattgaagt caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc
      121 agttctggct ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg
      181 tcggtgggaa ctatatcggc atgatgcccg ggaaaggtaa agagcaggat cactttgtcg
      241 cactcgatac ccaaccgaaa tatcggctgg acaatggcga tctgatgatc cacctgcaag
      301 cccccgatct cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg
      361 gaaaagtcta cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga
      421 tcgagcggcg ttttaccgat ctggtgaaaa aaggtagccg tttctggaac gtttccggcg
      481 ttgatgccaa cgtcagtatc agtggcgcga aggtgaaact ggaaagcttg gcggcactgg
      541 ttaacggtgc gattgccttc gattcaccag aagagtcgaa acctgccgag gcggaagata
      601 cctttggtct gtatgaagat ctggcccaca gccagcgtgg cgtaataata aaactggaac
      661 tgccgagtgg ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag
      721 tcggacagct gactaaactg gatttaaatc ctggtggtaa agtcacaggg gaaatgaccg
      781 ttgatcccag cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga
      841 aattatccct tagcgatgct aatctcagcg ccctgctgac cggcaaaacc ttcgagctgg
      901 tgcccggcga tggcgagcca cgcaaagaat tcgttgttgt gccaggcgaa aaagcactgc
      961 tgcatgaacc tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac ggtattgatg
     1021 cgggtcagcc gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca
     1081 ccagcaaagg cgtcaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag
     1141 gcgatagcaa atttgtcgtc aacagccgtg tcgatgtgaa ggtggggctg gatggcgttg
     1201 agtttctcgg tgccagcgcc tcagaatgga tcaatggcgg gatacgtatt ctgccgggcg
     1261 ataaaggtga gatgaaagcc agctatccac tgtatgccaa tttggaaaaa gcgctggaga
     1321 acagccttag cgatttaccc accacaaccg tgagtttgag tgcagagacg ctgccggatg
     1381 tgcaggcagg atcggtagtg ctgtaccgta aatttgaagt tggtgaagtg attaccgtgc
     1441 gtccgcgagc taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc
     1501 tgaccagcaa tagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg
     1561 gtctgaccgt acaggcatcc ccgctctcca gagcattaaa gggagccatt agcttcgaca
     1621 acctcagcgg tgccagcgcc agtcagcgta aaggcgataa gcgtattctg tatgcttccg
     1681 aaacagcggc ccgtgcggtt ggcgggcaga ttacgcttca cgctttcgat gccggaaaac
     1741 tggcggtcgg gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg
     1801 atctgattac cgcacgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc
     1861 agacctttgc tcgcggtggt acgcgcttct cagtggtcac accgcaaatt tcggcagctg
     1921 gcgttgagca tcttgatact aacctccagc cgtatatcaa cgtcgaacca ggccggggca
     1981 atcctcgccg cgactttgaa ttacaagagg ccaccattac tgattcgcgt tacctggatg
     2041 gcttaagcat tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc
     2101 tgttccgtgg tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc
     2161 gcgtgatgat tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg
     2221 tcttctggtt ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa
     2281 ccggcacctt taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc
     2341 cactggcacc gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag
     2401 agtggcgtga atggggaact gcgcttccca aataatgccc acctcgagca gatccggctg
     2461 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     2521 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     2581 ccggataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     2641 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2701 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2761 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2821 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2881 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2941 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3001 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     3061 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3121 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3181 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3241 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3301 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     3361 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3421 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3481 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3541 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3601 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3961 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     4621 cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg
     4681 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4741 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4801 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4861 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc
     4921 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     4981 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     5041 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     5101 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     5161 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     5221 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     5281 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     5341 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     5401 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     5461 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc
     5521 cgagatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
     5581 tctagaaata attttgttta actttaagaa ggagatatac atatggctct taacacacca
     5641 caaattacgc cgacaaaaaa gataacagtg agggcaatcg gcgaggaact gccgcgtggt
     5701 gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat aaattctcat
     5761 caaagtgcct attgtccgcg ctgtcaggca aaaattcgtg acgggcgcga ctggtcgcta
     5821 acgcgcctgg cggcaatggc cttcaccatg ctgttgctta tgccgtttgc ctggggcgaa
     5881 ccgctgttgc atatctggct gttaggcatt cgtattgacg ccaacgttat gcaaggcatc
     5941 tggcaaatga ccaaacaggg cgatgcgata acggggtcga tggtcttttt ctgcgttatt
     6001 ggtgccccgc tcattctggt gacctccata gcttatttat ggtttggtaa ccgactggga
     6061 atgaatttac gtccggtact gctgatgctt gagcgactta aagagtgggt aatgctcgat
     6121 atctacctgg tcggcattgg cgttgcttct ataaaggtac aggattatgc ccatatccag
     6181 gctggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt gacgttgtca
     6241 catcttaatg tcgaagaact gtgggagcga ttttatccgc agcgccccgc tacgcgtagg
     6301 gacgagaaac ttcgtgtctg cctagggtgc cattttaccg gctatccaga tcagcgtggt
     6361 cgctgcccgc gttgccatat cccgctacgc ctgcgtcgcc gtcatagtct gcaaaaatgc
     6421 tgggcggcgc tgttagcgtc aatcgtttta ttgttacctg ccaacctgtt gcctatttct
     6481 atcatttatc tgaacggagg acggcaggaa gatacaattc tttccggaat tatgtcgctg
     6541 gcaagtagca acattgcggt tgcaggaatc gtgtttatcg ccagtattct ggtcccgttt
     6601 actaaagtga tcgtcatgtt cactttactg ttgagcattc attttaaatg ccagcaaggt
     6661 ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggtcgctg gtcgatgctc
     6721 gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat cctcgctttt
     6781 actatgggac cggctgcgtt ttatttcggc gcagcggtaa ttttgactat tcttgctgtg
     6841 gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc ccgcttcgac
     6901 gactgaagcg cagattaaaa ataaacgccg tatctcacct ttctggctgc tgccgttcat
     6961 cgcgctaatg attgccagtt ggctgatttg ggacagttat caggaccggg gtaataccgt
     7021 caccatcgac tttatgtcgg cggatggtat tgttccaggc cgtacgcctg ttc
//
