LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20
                     /label=T->C mutation relative to reference sequence
     primer_bind     84..105
                     /label=oDCE3804
     primer_bind     154..171
                     /label=oDCE3802
     misc_feature    194
                     /label=G->C mutation relative to reference sequence
     misc_feature    197
                     /label=T->G mutation relative to reference sequence
     misc_feature    513
                     /label=C->T mutation relative to reference sequence
     misc_feature    914
                     /label=G->A mutation relative to reference sequence
     primer_bind     1062..1082
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     1905..1953
                     /label=oDCE3768
     primer_bind     complement(1905..1953)
                     /label=oDCE3769
     misc_feature    1929..1931
                     /label=L715N
     primer_bind     complement(2138..2159)
                     /label=oDCE3781
     primer_bind     complement(2485..2503)
                     /label=T7 Term
     terminator      2500..2546
                     /label=T7 terminator
     primer_bind     2949..2969
                     /label=oDCE3560
     CDS             2980..3639
                     /label=AmpR
     primer_bind     3568..3589
                     /label=oDCE3561
     rep_origin      3737..4419
                     /label=ColE1 origin
     primer_bind     4183..4204
                     /label=oDCE3562
     primer_bind     4796..4815
                     /label=oDCE3563
     primer_bind     complement(4969..4990)
                     /label=oDCE3648
     primer_bind     5384..5403
                     /label=oDCE3564
     primer_bind     5528..5547
                     /label=T7
     misc_feature    5608..6891
                     /label=YebS
     CDS             5608..6891
                     /label=Translation 5608-6891
     primer_bind     6055..6073
                     /label=oDCE3554
     misc_feature    6306
                     /label=T->C mutation relative to reference sequence
     misc_feature    6309
                     /label=T->A mutation relative to reference sequence
     misc_feature    6579
                     /label=A->C mutation relative to reference sequence
     primer_bind     6661..6681
                     /label=oDCE3555
     misc_feature    join(6860..7073,1..2420)
                     /label=YebT
     CDS             join(6860..7073,1..2420)
                     /label=Translation 6860-2420
ORIGIN
        1 aagtcggaac agtgcaggac atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
       61 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctggtga
      121 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
      181 tcggcatgat gcccgggaaa ggtaaagagc aggatcactt tgtcgcactc gatacccaac
      241 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gatctcggtt
      301 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
      361 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
      421 ccgatctggt gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca
      481 gtatcagtgg cgcgaaggtg aaactggaaa gcttggcggc actggttaac ggtgcgattg
      541 ccttcgattc accagaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
      601 aagatctggc ccacagccag cgtggcgtaa taataaaact ggaactgccg agtggggccg
      661 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
      721 aactggattt aaatcctggt ggtaaagtca caggggaaat gaccgttgat cccagcgtcg
      781 ttaccctgct tcgtgaaaat acccgcatcg aattacgcaa cccgaaatta tcccttagcg
      841 atgctaatct cagcgccctg ctgaccggca aaaccttcga gctggtgccc ggcgatggcg
      901 agccacgcaa agaattcgtt gttgtgccag gcgaaaaagc actgctgcat gaacctgatg
      961 ttctgacgct gaccctgacc gcgccggaaa gttacggtat tgatgcgggt cagccgctca
     1021 ttcttcacgg cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca
     1081 cctttaccgt cgccatcgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg
     1141 tcgtcaacag ccgtgtcgat gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca
     1201 gcgcctcaga atggatcaat ggcgggatac gtattctgcc gggcgataaa ggtgagatga
     1261 aagccagcta tccactgtat gccaatttgg aaaaagcgct ggagaacagc cttagcgatt
     1321 tacccaccac aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg
     1381 tagtgctgta ccgtaaattt gaagttggtg aagtgattac cgtgcgtccg cgagctaacg
     1441 cgtttgatat cgatctgcat attaagccgg agtatcgcaa ccttctgacc agcaatagcg
     1501 tgttctgggc agaaggcggg gcgaaagttc agctgaatgg tagtggtctg accgtacagg
     1561 catccccgct ctccagagca ttaaagggag ccattagctt cgacaacctc agcggtgcca
     1621 gcgccagtca gcgtaaaggc gataagcgta ttctgtatgc ttccgaaaca gcggcccgtg
     1681 cggttggcgg gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc
     1741 caattcgcta tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcac
     1801 gcaatgaagt acaggcaaag gcggtacttt acccggaata tgtccagacc tttgctcgcg
     1861 gtggtacgcg cttctcagtg gtcacaccgc aaatttcggc agctggcgtt gagcatcttg
     1921 atactatcaa tcagccgtat atcaacgtcg aaccaggccg gggcaatcct cgccgcgact
     1981 ttgaattaca agaggccacc attactgatt cgcgttacct ggatggctta agcattattg
     2041 ttgaagcgcc ggaagccggt tcgttaggca tcggtacgcc tgtgctgttc cgtggtctgg
     2101 aagtcggtac ggttacagga atgacgctgg ggacattgtc agatcgcgtg atgattgcga
     2161 tgcgcatcag taaacgctat caacacctgg tgcgtaacaa ttccgtcttc tggttggcat
     2221 cgggttacag tctggacttt ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc
     2281 agtttatccg tggcggcatc gccttcgcca cgcctccggg tacgccactg gcaccgaaag
     2341 cccaggaagg caaacacttc ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg
     2401 gaactgcgct tcccaaataa tgcccacctc gagcagatcc ggctgctaac aaagcccgaa
     2461 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     2521 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga taattcttga
     2581 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2641 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2701 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2761 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2821 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2881 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2941 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3001 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     3061 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3121 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3181 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3241 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3301 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     3361 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3421 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3481 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3541 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3601 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3661 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4561 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4621 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4681 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4861 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     4921 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     4981 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     5041 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     5101 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     5161 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     5221 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     5281 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     5341 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     5401 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     5461 caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tctcgatccc
     5521 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt
     5581 gtttaacttt aagaaggaga tatacatatg gctcttaaca caccacaaat tacgccgaca
     5641 aaaaagataa cagtgagggc aatcggcgag gaactgccgc gtggtgatta ccaacgttgc
     5701 ccgcaatgtg acatgctgtt tagcctgccc gagataaatt ctcatcaaag tgcctattgt
     5761 ccgcgctgtc aggcaaaaat tcgtgacggg cgcgactggt cgctaacgcg cctggcggca
     5821 atggccttca ccatgctgtt gcttatgccg tttgcctggg gcgaaccgct gttgcatatc
     5881 tggctgttag gcattcgtat tgacgccaac gttatgcaag gcatctggca aatgaccaaa
     5941 cagggcgatg cgataacggg gtcgatggtc tttttctgcg ttattggtgc cccgctcatt
     6001 ctggtgacct ccatagctta tttatggttt ggtaaccgac tgggaatgaa tttacgtccg
     6061 gtactgctga tgcttgagcg acttaaagag tgggtaatgc tcgatatcta cctggtcggc
     6121 attggcgttg cttctataaa ggtacaggat tatgcccata tccaggctgg tgtcggcttg
     6181 ttctcttttg tggcgttggt gattttaacg acggtgacgt tgtcacatct taatgtcgaa
     6241 gaactgtggg agcgatttta tccgcagcgc cccgctacgc gtagggacga gaaacttcgt
     6301 gtctgcctag ggtgccattt taccggctat ccagatcagc gtggtcgctg cccgcgttgc
     6361 catatcccgc tacgcctgcg tcgccgtcat agtctgcaaa aatgctgggc ggcgctgtta
     6421 gcgtcaatcg ttttattgtt acctgccaac ctgttgccta tttctatcat ttatctgaac
     6481 ggaggacggc aggaagatac aattctttcc ggaattatgt cgctggcaag tagcaacatt
     6541 gcggttgcag gaatcgtgtt tatcgccagt attctggtcc cgtttactaa agtgatcgtc
     6601 atgttcactt tactgttgag cattcatttt aaatgccagc aaggtttacg cacacgcatt
     6661 ctgttactgc ggatggtgac ctggattggt cgctggtcga tgctcgacct gtttgtcata
     6721 tctttaacca tgtcgctgat taatcgcgat cagatcctcg cttttactat gggaccggct
     6781 gcgttttatt tcggcgcagc ggtaattttg actattcttg ctgtggaatg gctggacagc
     6841 cgcttacttt gggatgcaca tgagtcagga aacgcccgct tcgacgactg aagcgcagat
     6901 taaaaataaa cgccgtatct cacctttctg gctgctgccg ttcatcgcgc taatgattgc
     6961 cagttggctg atttgggaca gttatcagga ccggggtaat accgtcacca tcgactttat
     7021 gtcggcggat ggtattgttc caggccgtac gcctgttcgt tatcagggcg ttg
//
