LOCUS       Exported                7073 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14
                     /label=T->C mutation relative to reference sequence
     primer_bind     78..99
                     /label=oDCE3804
     primer_bind     148..165
                     /label=oDCE3802
     misc_feature    188
                     /label=G->C mutation relative to reference sequence
     misc_feature    191
                     /label=T->G mutation relative to reference sequence
     misc_feature    507
                     /label=C->T mutation relative to reference sequence
     misc_feature    908
                     /label=G->A mutation relative to reference sequence
     primer_bind     1056..1076
                     /label=oDCE3777_BAD-SEQUENCING
     primer_bind     1144..1163
                     /label=oDCE3608
     misc_feature    1223
                     /label=A->C mutation relative to reference sequence
     primer_bind     complement(1239..1263)
                     /label=oDCE3803
     primer_bind     complement(1309..1331)
                     /label=oDCE3806
     misc_feature    1442
                     /label=T->C mutation relative to reference sequence
     misc_feature    1574..1575
                     /label=AT->TC mutations relative to reference sequence
     misc_feature    1757
                     /label=T->C mutation relative to reference sequence
     primer_bind     1892..1911
                     /label=oDCE3805
     misc_feature    2009
                     /label=Silent point mutation relative to reference seq
                     /label=Silent point mutation relative to reference sequence
     primer_bind     2260..2300
                     /label=oDCE3774
     primer_bind     complement(2260..2300)
                     /label=oDCE3775
     misc_feature    2280..2282
                     /label=I834N
     primer_bind     complement(2393..2452)
                     /label=oDCE3807
     primer_bind     complement(2479..2497)
                     /label=T7 Term
     terminator      2494..2540
                     /label=T7 terminator
     primer_bind     2943..2963
                     /label=oDCE3560
     CDS             2974..3633
                     /label=AmpR
     primer_bind     3562..3583
                     /label=oDCE3561
     rep_origin      3731..4413
                     /label=ColE1 origin
     primer_bind     4177..4198
                     /label=oDCE3562
     primer_bind     4790..4809
                     /label=oDCE3563
     primer_bind     complement(4963..4984)
                     /label=oDCE3648
     primer_bind     5378..5397
                     /label=oDCE3564
     primer_bind     5522..5541
                     /label=T7
     misc_feature    5602..6885
                     /label=YebS
     CDS             5602..6885
                     /label=Translation 5602-6885
     primer_bind     6049..6067
                     /label=oDCE3554
     misc_feature    6300
                     /label=T->C mutation relative to reference sequence
     misc_feature    6303
                     /label=T->A mutation relative to reference sequence
     misc_feature    6573
                     /label=A->C mutation relative to reference sequence
     primer_bind     6655..6675
                     /label=oDCE3555
     misc_feature    join(6854..7073,1..2414)
                     /label=YebT
     CDS             join(6854..7073,1..2414)
                     /label=Translation 6854-2414
ORIGIN
        1 gaacagtgca ggacatcagc ctcagcgacg atcttcgtaa gattgaagtc aaggtcagca
       61 tcaagtccga tatgaaagat gcgctgcgcg aagagactca gttctggctg gtgacgccaa
      121 aagcatcgtt ggcaggtgtc tccgggctgg acgccctcgt cggtgggaac tatatcggca
      181 tgatgcccgg gaaaggtaaa gagcaggatc actttgtcgc actcgatacc caaccgaaat
      241 atcggctgga caatggcgat ctgatgatcc acctgcaagc ccccgatctc ggttcgctga
      301 acagcggttc attggtctat ttccgcaaga tcccggtggg aaaagtctac gactatgcca
      361 tcaatcccaa caagcaaggc gtggtgattg atgtcctgat cgagcggcgt tttaccgatc
      421 tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt tgatgccaac gtcagtatca
      481 gtggcgcgaa ggtgaaactg gaaagcttgg cggcactggt taacggtgcg attgccttcg
      541 attcaccaga agagtcgaaa cctgccgagg cggaagatac ctttggtctg tatgaagatc
      601 tggcccacag ccagcgtggc gtaataataa aactggaact gccgagtggg gccggattaa
      661 ccgccgactc gacgccgtta atgtatcagg ggctggaagt cggacagctg actaaactgg
      721 atttaaatcc tggtggtaaa gtcacagggg aaatgaccgt tgatcccagc gtcgttaccc
      781 tgcttcgtga aaatacccgc atcgaattac gcaacccgaa attatccctt agcgatgcta
      841 atctcagcgc cctgctgacc ggcaaaacct tcgagctggt gcccggcgat ggcgagccac
      901 gcaaagaatt cgttgttgtg ccaggcgaaa aagcactgct gcatgaacct gatgttctga
      961 cgctgaccct gaccgcgccg gaaagttacg gtattgatgc gggtcagccg ctcattcttc
     1021 acggcgtgca ggtaggccag gttatcgatc gtaaactcac cagcaaaggc gtcaccttta
     1081 ccgtcgccat cgagcctcag catcgcgaac tggtaaaagg cgatagcaaa tttgtcgtca
     1141 acagccgtgt cgatgtgaag gtggggctgg atggcgttga gtttctcggt gccagcgcct
     1201 cagaatggat caatggcggg atccgtattc tgccgggcga taaaggtgag atgaaagcca
     1261 gctatccact gtatgccaat ttggaaaaag cgctggagaa cagccttagc gatttaccca
     1321 ccacaaccgt gagtttgagt gcagagacgc tgccggatgt gcaggcagga tcggtagtgc
     1381 tgtaccgtaa atttgaagtt ggtgaagtga ttaccgtgcg tccgcgagct aacgcgtttg
     1441 acatcgatct gcatattaag ccggagtatc gcaaccttct gaccagcaat agcgtgttct
     1501 gggcagaagg cggggcgaaa gttcagctga atggtagtgg tctgaccgta caggcatccc
     1561 cgctctccag agctctaaag ggagccatta gcttcgacaa cctcagcggt gccagcgcca
     1621 gtcagcgtaa aggcgataag cgtattctgt atgcttccga aacagcggcc cgtgcggttg
     1681 gcgggcagat tacgcttcac gctttcgatg ccggaaaact ggcggtcggg atgccaattc
     1741 gctatctcgg tattgacatc gggcaaatcc agacgctgga tctgattacc gcacgcaatg
     1801 aagtacaggc aaaggcggta ctttacccgg aatatgtcca gacctttgct cgcggtggta
     1861 cgcgcttctc agtggtcaca ccgcaaattt cggcagctgg cgttgagcat cttgatacta
     1921 tcctccagcc gtatatcaac gtcgaaccag gccggggcaa tcctcgccgc gactttgaat
     1981 tacaagaggc caccattact gattcgcggt acctggatgg cttaagcatt attgttgaag
     2041 cgccggaagc cggttcgtta ggcatcggta cgcctgtgct gttccgtggt ctggaagtcg
     2101 gtacggttac aggaatgacg ctggggacat tgtcagatcg cgtgatgatt gcgatgcgca
     2161 tcagtaaacg ctatcaacac ctggtgcgta acaattccgt cttctggttg gcatcgggtt
     2221 acagtctgga ctttggtctg acgggcggcg tagtgaaaac cggcaccttt aaccagttta
     2281 atcgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg aaagcccagg
     2341 aaggcaaaca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa tggggaactg
     2401 cgcttcccaa ataatgccca cctcgagcag atccggctgc taacaaagcc cgaaaggaag
     2461 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     2521 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataattc ttgaagacga
     2581 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2641 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3001 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     3061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3301 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3541 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3601 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4561 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4621 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4681 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     4861 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     4921 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     4981 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     5041 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     5101 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     5161 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     5221 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     5281 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     5341 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     5401 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     5461 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
     5521 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
     5581 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
     5641 ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa
     5701 tgtgacatgc tgtttagcct gcccgagata aattctcatc aaagtgccta ttgtccgcgc
     5761 tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc
     5821 ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac cgctgttgca tatctggctg
     5881 ttaggcattc gtattgacgc caacgttatg caaggcatct ggcaaatgac caaacagggc
     5941 gatgcgataa cggggtcgat ggtctttttc tgcgttattg gtgccccgct cattctggtg
     6001 acctccatag cttatttatg gtttggtaac cgactgggaa tgaatttacg tccggtactg
     6061 ctgatgcttg agcgacttaa agagtgggta atgctcgata tctacctggt cggcattggc
     6121 gttgcttcta taaaggtaca ggattatgcc catatccagg ctggtgtcgg cttgttctct
     6181 tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt cgaagaactg
     6241 tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact tcgtgtctgc
     6301 ctagggtgcc attttaccgg ctatccagat cagcgtggtc gctgcccgcg ttgccatatc
     6361 ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct gggcggcgct gttagcgtca
     6421 atcgttttat tgttacctgc caacctgttg cctatttcta tcatttatct gaacggagga
     6481 cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa cattgcggtt
     6541 gcaggaatcg tgtttatcgc cagtattctg gtcccgttta ctaaagtgat cgtcatgttc
     6601 actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg cattctgtta
     6661 ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt catatcttta
     6721 accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc ggctgcgttt
     6781 tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga cagccgctta
     6841 ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa
     6901 taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga ttgccagttg
     6961 gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact ttatgtcggc
     7021 ggatggtatt gttccaggcc gtacgcctgt tcgttatcag ggcgttgaag tcg
//
