LOCUS       pUCDMp10                4880 bp ds-DNA     circular     22-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_recomb     415..448
                     /label="LoxP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      complement(470..752)
                     /label="HSV-1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    937..938
                     /label="SpeI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    937..942
                     /label="SpeI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        943..1822
                     /label="hr1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        1823..2289
                     /label="HSP70"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2289..2309
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2290..2309
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2310..2387
                     /label=""Multiple cloning site""
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     3'UTR           2388..3053
                     /label="p10"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     polyA_signal    complement(3122..3313)
                     /label=""SV40 late polyA""
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             3835..4491
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 AATTCTGTCA GCCGTTAAGT GTTCCTGTGT CACTCAAAAT TGCTTTGAGA GGCTCTAAGG
       61 GCTTCTCAGT GCGTTACATC CCTGGCTTGT TGTCCACAAC CGTTAAACCT TAAAAGCTTT
      121 AAAAGCCTTA TATATTCTTT TTTTTCTTAT AAAACTTAAA ACCTTAGAGG CTATTTAAGT
      181 TGCTGATTTA TATTAATTTT ATTGTTCAAA CATGAGAGCT TAGTACGTGA AACATGAGAG
      241 CTTAGTACGT TAGCCATGAG AGCTTAGTAC GTTAGCCATG AGGGTTTAGT TCGTTAAACA
      301 TGAGAGCTTA GTACGTTAAA CATGAGAGCT TAGTACGTGA AACATGAGAG CTTAGTACGT
      361 ACTATCAACA GGTTGAACTG CTGATCAACA GATCCTCTAC GCGGCCGCGG TACCATAACT
      421 TCGTATAGCA TACATTATAC GAAGTTATCT GGTTTAAACG TACCCGTAGT GGCTATGGCA
      481 GGGCTTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGTTGG
      541 GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGTCCCAAT GGGGTCTCGG TGGGGTATCG
      601 ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCCGTG
      661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT
      721 CCGTGTTTCA GTTAGCCTCC CCCATCTCCC GGTACCGCAT GCTATGCATC AGCTGCTAGC
      781 ACCATGGCTC GAGATCCCGG GTGATCAAGT CTTCGTCGAG TGATTGTAAA TAAAATGTAA
      841 TTTACAGTAT AGTATTTTAA TTAATATACA AATGATTTGA TAATAATTCT TATTTAACTA
      901 TAATATATTG TGTTGGGTTG AATTAAAGGT CCGTATACTA GTATCGATGA TTGACCCCAA
      961 CAAAAGATTT ATAATTAATC ATAATCACGA ACAACAACAA GTCAATGAAA CAAATAAACA
     1021 AGTTGTCGAT AAAACATTCA TAAATGACAC AGCAACATAC AATTCTTGCA TAATAAAAAT
     1081 TTAAATGACA TCATATTTGA GAATAACAAA TGACATTATC CCTCGATTGT GTTTTACAAG
     1141 TAGAATTCTA CCCGTAAAGC GAGTTTAGTT TTGAAAAACA AATGACATCA TTTGTATAAT
     1201 GACATCATCC CCTGATTGTG TTTTACAAGT AGAATTCTAT CCGTAAAGCG AGTTCAGTTT
     1261 TGAAAACAAA TGAGTCATAC CTAAACACGT TAATAATCTT CTGATATCAG CTTATGACTC
     1321 AAGTTATGAG CCGTGTGCAA AACATGAGAT AAGTTTATGA CATCATCCAC TGATCGTGCG
     1381 TTACAAGTAG AATTCTACTC GTAAAGCCAG TTCGGTTATG AGCCGTGTGC AAAACATGAC
     1441 ATCAGCTTAT GACTCATACT TGATTGTGTT TTACGCGTAG AATTCTACTC GTAAAGCGAG
     1501 TTCGGTTATG AGCCGTGTGC AAAACATGAC ATCAGCTTAT GAGTCATAAT TAATCGTGCG
     1561 TTACAAGTAG AATTCTACTC GTAAAGCGAG TTGAAGGATC ATATTTAGTT GCGTTTATGA
     1621 GATAAGATTG AAAAGCGTGT AAAATGTTTC CCGCGCTTGG CACAACTATT TACAATGCGG
     1681 CCAAGTTATA AAAGATTCTA ATCTGATATG TTTTAAAACA CCTTTGCGGC CCGAGTTGTT
     1741 TGCGTACGTG ACTAGCGAAG AAGATGTGTG GACCGCAGAA CAGATAGTAA AACAAAACCC
     1801 TAGTATTGGA GCAATAATCG ATCCTAGAAT CCCAAAACAA ACTGGTTATT GTGGTAGGTC
     1861 ATTTGTTTGG CAGAAAGAAA ACTCGAGAAA TTTCTCTGGC CGTTATTCGT TATTCTCTCT
     1921 TTTCTTTTTG GGTCTCTCCC TCTCTGCACT AATGCTCTCT CACTCTGTCA CACAGTAAAC
     1981 GGCATACTGC TCTCGTTGGT TCGAGAGAGC GCGCCTCGAA TGTTCGCGAA AAGAGCGCCG
     2041 GAGTATAAAT AGAGGCGCTT CGTCTACGGA GCGACAATTC AATTCAAACA AGCAAAGTGA
     2101 ACACGTCGCT AAGCGAAAGC TAAGCAAATA AACAAGCGCA GCTGAACAAG CTAAACAATC
     2161 TGCAGTAAAG TGCAAGTTAA AGTGAATCAA TTAAAAGTAA CCAGCAACCA AGTAAATCAA
     2221 CTGCAACTAC TGAAATCTGC CAAGAAGTAA TTATTGAATA CAAGAAGAGA ACTCTGAATA
     2281 GGGAATTGGT AATACGACTC ACTATAGGGG GATCCCGGTC CGAAGCGCGC GGAATTCAAA
     2341 GGCCTACGTC GACGAGCTCA CTAGTCGCGG CCGCTTTCGA ATCTAGAATG AATCGTTTTT
     2401 AAAATAACAA ATCAATTGTT TTATAATATT CGTACGATTC TTTGATTATG TAATAAAATG
     2461 TGATCATTAG GAAGATTACG AAAAATATAA AAAATATGAG TTCTGTGTGT ATAACAAATG
     2521 CTGTAAACGC CACAATTGTG TTTGTTGCAA ATAAACCCAT GATTATTTGA TTAAAATTGT
     2581 TGTTTTCTTT GTTCATAGAC AATAGTGTGT TTTGCCTAAA CGTGTACTGC ATAAACTCCA
     2641 TGCGAGTGTA TAGCGAGCTA GTGGCTAACG CTTGCCCCAC CAAAGTAGAT TCGTCAAAAT
     2701 CCTCAATTTC ATCACCCTCC TCCAAGTTTA ACATTTGGCC GTCGGAATTA ACTTCTAAAG
     2761 ATGCCACATA ATCTAATAAA TGAAATAGAG ATTCAAACGT GGCGTCATCG TCCGTTTCGA
     2821 CCATTTCCGA AAAGAACTCG GGCATAAACT CTATGATTTC TCTGGACGTG GTGTTGTCGA
     2881 AACTCTCAAA GTACGCAGTC AGGAACGTGC GCGACATGTC GTCGGGAAAC TCGCGCGGAA
     2941 ACATGTTGTT GTAACCGAAC GGGTCCCATA GCGCCAAAAC CAAATCTGCC AGCGTCAATA
     3001 GAATGAGCAC GATGCCGACA ATGGAGCTGG CTTGGATAGC GATTCGAGTT AACAAGCTTG
     3061 TCGAGAAGTA CTAGAGGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT
     3121 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG
     3181 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
     3241 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
     3301 CTTATCATGT CTGGATCTGA TCACTGCTTG AGCCTAGAAG ATCCGGCTGC TAACAAAGCC
     3361 CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTATCATA ACCTAACCCC
     3421 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGACCCT AGGGGCCTCT AAACGGGTCT
     3481 TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATC TGAACAGGAG GGACAGCTGA
     3541 TAGAAACAGA AGCCACTGGA GCACCTCAAA AACACCATCA TACACTAAAT CAGTAAGTTG
     3601 GCAGCATCAC CCGACGCACT TTGCGCCGAA TAAATACCTG TGACGGAAGA TCACTTCGCA
     3661 GAATAAATAA ATCCTGGTGT CCCTGTTGAT ACCGGGAAGC CCTGGGCCAA CTTTTGGCGA
     3721 AAATGAGACG TTGATCGGCA CGTAAGAGGT TCCAACTTTC ACCATAATGA AATAAGATCA
     3781 CTACCGGGCG TATTTTTTGA GTTATCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG
     3841 AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT
     3901 GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG
     3961 GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT
     4021 CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG
     4081 GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT
     4141 TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA
     4201 GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG
     4261 TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT
     4321 ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG
     4381 GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC
     4441 AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
     4501 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG TGATAATAAG
     4561 CGGATGAATG GCAGAAATTC GAAAGCAAAT TCGACCCGGT CGTCGGTTCA GGGCAGGGTC
     4621 GTTAAATAGC CGCTTATGTC TATTGCTGGT TTACCGGTTT ATTGACTACC GGAAGCAGTG
     4681 TGACCGTGTG CTTCTCAAAT GCCTGAGGCC AGTTTGCTCA GGCTCTCCCC GTGGAGGTAA
     4741 TAATTGACGA TATGATCATT TATTCTGCCT CCCAGCTGAC ATTCATCCGG GGTCAGCACC
     4801 GTTTCTGCGG ACTGGCTTTC TACGTGTTCC GCTTCCTTTA GCAGCCCTTG CGCCCTGAGT
     4861 GCTTGCGGCA GCGTGAAGCT
//