LOCUS       pSPLp10                 4715 bp ds-DNA     circular     22-MAR-2021
DEFINITION  .
SOURCE      . (unknown)                                                        
  ORGANISM  . . .                                                              
FEATURES             Location/Qualifiers
     misc_recomb     416..449
                     /label="LoxP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      complement(471..753)
                     /label="HSV-1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        944..1823
                     /label="hr1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        1824..2290
                     /label="HSP70"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        2291..2310
                     /label="""T7 promoter"""
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2311..2388
                     /label=""Multiple cloning site""
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     3'UTR           2389..3054
                     /label="p10"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      3078..3318
                     /label="SV40"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     polyA_signal    complement(3123..3314)
                     /label=""SV40 late polyA""
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      3293..3336
                     /label="""T7 terminator"""
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(3468..4259)
                     /label="SmR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 GAATTCTGTC AGCCGTTAAG TGTTCCTGTG TCACTCAAAA TTGCTTTGAG AGGCTCTAAG
       61 GGCTTCTCAG TGCGTTACAT CCCTGGCTTG TTGTCCACAA CCGTTAAACC TTAAAAGCTT
      121 TAAAAGCCTT ATATATTCTT TTTTTTCTTA TAAAACTTAA AACCTTAGAG GCTATTTAAG
      181 TTGCTGATTT ATATTAATTT TATTGTTCAA ACATGAGAGC TTAGTACGTG AAACATGAGA
      241 GCTTAGTACG TTAGCCATGA GAGCTTAGTA CGTTAGCCAT GAGGGTTTAG TTCGTTAAAC
      301 ATGAGAGCTT AGTACGTTAA ACATGAGAGC TTAGTACGTG AAACATGAGA GCTTAGTACG
      361 TACTATCAAC AGGTTGAACT GCTGATCAAC AGATCCTCTA CGCGGCCGCG GTACCATAAC
      421 TTCGTATAGC ATACATTATA CGAAGTTATC TGGTTTAAAC GTACCCGTAG TGGCTATGGC
      481 AGGGCTTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGTTG
      541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGTCCCAA TGGGGTCTCG GTGGGGTATC
      601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCCGT
      661 GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT
      721 TCCGTGTTTC AGTTAGCCTC CCCCATCTCC CGGTACCGCA TGCTATGCAT CAGCTGCTAG
      781 CACCATGGCT CGAGATCCCG GGTGATCAAG TCTTCGTCGA GTGATTGTAA ATAAAATGTA
      841 ATTTACAGTA TAGTATTTTA ATTAATATAC AAATGATTTG ATAATAATTC TTATTTAACT
      901 ATAATATATT GTGTTGGGTT GAATTAAAGG TCCGTATACT AGTATCGATG ATTGACCCCA
      961 ACAAAAGATT TATAATTAAT CATAATCACG AACAACAACA AGTCAATGAA ACAAATAAAC
     1021 AAGTTGTCGA TAAAACATTC ATAAATGACA CAGCAACATA CAATTCTTGC ATAATAAAAA
     1081 TTTAAATGAC ATCATATTTG AGAATAACAA ATGACATTAT CCCTCGATTG TGTTTTACAA
     1141 GTAGAATTCT ACCCGTAAAG CGAGTTTAGT TTTGAAAAAC AAATGACATC ATTTGTATAA
     1201 TGACATCATC CCCTGATTGT GTTTTACAAG TAGAATTCTA TCCGTAAAGC GAGTTCAGTT
     1261 TTGAAAACAA ATGAGTCATA CCTAAACACG TTAATAATCT TCTGATATCA GCTTATGACT
     1321 CAAGTTATGA GCCGTGTGCA AAACATGAGA TAAGTTTATG ACATCATCCA CTGATCGTGC
     1381 GTTACAAGTA GAATTCTACT CGTAAAGCCA GTTCGGTTAT GAGCCGTGTG CAAAACATGA
     1441 CATCAGCTTA TGACTCATAC TTGATTGTGT TTTACGCGTA GAATTCTACT CGTAAAGCGA
     1501 GTTCGGTTAT GAGCCGTGTG CAAAACATGA CATCAGCTTA TGAGTCATAA TTAATCGTGC
     1561 GTTACAAGTA GAATTCTACT CGTAAAGCGA GTTGAAGGAT CATATTTAGT TGCGTTTATG
     1621 AGATAAGATT GAAAAGCGTG TAAAATGTTT CCCGCGCTTG GCACAACTAT TTACAATGCG
     1681 GCCAAGTTAT AAAAGATTCT AATCTGATAT GTTTTAAAAC ACCTTTGCGG CCCGAGTTGT
     1741 TTGCGTACGT GACTAGCGAA GAAGATGTGT GGACCGCAGA ACAGATAGTA AAACAAAACC
     1801 CTAGTATTGG AGCAATAATC GATCCTAGAA TCCCAAAACA AACTGGTTAT TGTGGTAGGT
     1861 CATTTGTTTG GCAGAAAGAA AACTCGAGAA ATTTCTCTGG CCGTTATTCG TTATTCTCTC
     1921 TTTTCTTTTT GGGTCTCTCC CTCTCTGCAC TAATGCTCTC TCACTCTGTC ACACAGTAAA
     1981 CGGCATACTG CTCTCGTTGG TTCGAGAGAG CGCGCCTCGA ATGTTCGCGA AAAGAGCGCC
     2041 GGAGTATAAA TAGAGGCGCT TCGTCTACGG AGCGACAATT CAATTCAAAC AAGCAAAGTG
     2101 AACACGTCGC TAAGCGAAAG CTAAGCAAAT AAACAAGCGC AGCTGAACAA GCTAAACAAT
     2161 CTGCAGTAAA GTGCAAGTTA AAGTGAATCA ATTAAAAGTA ACCAGCAACC AAGTAAATCA
     2221 ACTGCAACTA CTGAAATCTG CCAAGAAGTA ATTATTGAAT ACAAGAAGAG AACTCTGAAT
     2281 AGGGAATTGG TAATACGACT CACTATAGGG GGATCCCGGT CCGAAGCGCG CGGAATTCAA
     2341 AGGCCTACGT CGACGAGCTC ACTAGTCGCG GCCGCTTTCG AATCTAGAAT GAATCGTTTT
     2401 TAAAATAACA AATCAATTGT TTTATAATAT TCGTACGATT CTTTGATTAT GTAATAAAAT
     2461 GTGATCATTA GGAAGATTAC GAAAAATATA AAAAATATGA GTTCTGTGTG TATAACAAAT
     2521 GCTGTAAACG CCACAATTGT GTTTGTTGCA AATAAACCCA TGATTATTTG ATTAAAATTG
     2581 TTGTTTTCTT TGTTCATAGA CAATAGTGTG TTTTGCCTAA ACGTGTACTG CATAAACTCC
     2641 ATGCGAGTGT ATAGCGAGCT AGTGGCTAAC GCTTGCCCCA CCAAAGTAGA TTCGTCAAAA
     2701 TCCTCAATTT CATCACCCTC CTCCAAGTTT AACATTTGGC CGTCGGAATT AACTTCTAAA
     2761 GATGCCACAT AATCTAATAA ATGAAATAGA GATTCAAACG TGGCGTCATC GTCCGTTTCG
     2821 ACCATTTCCG AAAAGAACTC GGGCATAAAC TCTATGATTT CTCTGGACGT GGTGTTGTCG
     2881 AAACTCTCAA AGTACGCAGT CAGGAACGTG CGCGACATGT CGTCGGGAAA CTCGCGCGGA
     2941 AACATGTTGT TGTAACCGAA CGGGTCCCAT AGCGCCAAAA CCAAATCTGC CAGCGTCAAT
     3001 AGAATGAGCA CGATGCCGAC AATGGAGCTG GCTTGGATAG CGATTCGAGT TAACAAGCTT
     3061 GTCGAGAAGT ACTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
     3121 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
     3181 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     3241 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     3301 TCTTATCATG TCTGGATCTG ATCACTGCTT GAGCCTAGAA GATCCGGCTG CTAACAAAGC
     3361 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTATCAT AACCCCTAGG
     3421 AGTCCAAGCG AGCTCGATAT CCGTCGGCTT GAACGAATTG TTAGACATTA TTTGCCGACT
     3481 ACCTTGGTGA TCTCGCCTTT CACGTAGTGG ACAAATTCTT CCAACTGATC TGCGCGCGAG
     3541 GCCAAGCGAT CTTCTTCTTG TCCAAGATAA GCCTGTCTAG CTTCAAGTAT GACGGGCTGA
     3601 TACTGGGCCG GCAGGCGCTC CATTGCCCAG TCGGCAGCGA CATCCTTCGG CGCGATTTTG
     3661 CCGGTTACTG CGCTGTACCA AATGCGGGAC AACGTAAGCA CTACATTTCG CTCATCGCCA
     3721 GCCCAGTCGG GCGGCGAGTT CCATAGCGTT AAGGTTTCAT TTAGCGCCTC AAATAGATCC
     3781 TGTTCAGGAA CCGGATCAAA GAGTTCCTCC GCCGCTGGAC CTACCAAGGC AACGCTATGT
     3841 TCTCTTGCTT TTGTCAGCAA GATAGCCAGA TCAATGTCGA TCGTGGCTGG CTCGAAGATA
     3901 CCTGCAAGAA TGTCATTGCG CTGCCATTCT CCAAATTGCA GTTCGCGCTT AGCTGGATAA
     3961 CGCCACGGAA TGATGTCGTC GTGCACAACA ATGGTGACTT CTACAGCGCG GAGAATCTCG
     4021 CTCTCTCCAG GGGAAGCCGA AGTTTCCAAA AGGTCGTTGA TCAAAGCTCG CCGCGTTGTT
     4081 TCATCAAGCC TTACGGTCAC CGTAACCAGC AAATCAATAT CACTGTGTGG CTTCAGGCCG
     4141 CCATCCACTG CGGAGCCGTA CAAATGTACG GCCAGCAACG TCGGTTCGAG ATGGCGCTCG
     4201 ATGACGCCAA CTACCTCTGA TAGTTGAGTC GATACTTCGG CGATCACCGC TTCCCTCATG
     4261 ATGTTTAACT TTGTTTTAGG GCGACTGCCC TGCTGCGTAA CATCGTTGCT GCTCCATAAC
     4321 ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG ATGCCCGAGG CATAGACTGT
     4381 ACCCCAAAAA AACAGTCATA ACAAGCCATG AAAACCGCCA CTGCGCCGTT ACCACCGCTG
     4441 CGTTCGGTCA AGGTTCTGGA CCAGTTGCGT GAGCGCATAC GCTACTTACC GGTTTATTGA
     4501 CTACCGGAAG CAGTGTGACC GTGTGCTTCT CAAATGCCTG AGGCCAGTTT GCTCAGGCTC
     4561 TCCCCGTGGA GGTAATAATT GACGATATGA TCATTTATTC TGCCTCCCAG CTGACATTCA
     4621 TCCGGGGTCA GCACCGTTTC TGCGGACTGG CTTTCTACGT GTTCCGCTTC CTTTAGCAGC
     4681 CCTTGCGCCC TGAGTGCTTG CGGCAGCGTG AAGCT
//