LOCUS       Exported               10009 bp ds-DNA     circular SYN 16-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   John James
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 16, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        830..934
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             935..1795
                     /label=bla (AmpR)
                     /note="color: #993366; direction: RIGHT"
     rep_origin      1966..2554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        2800..3129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3329..3786
                     /label=gpt(fs)
                     /note="color: #993366; direction: RIGHT"
     intron          4263..4328
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     polyA_signal    4903..5037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             5206..5885
                     /label=5'-LTR
                     /note="color: #ffe4c4"
     misc_feature    5886..6011
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     CDS             6012..7214
                     /label=HIV genes
                     /note="color: #993366; direction: RIGHT"
     misc_feature    7251..7368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        7521..7928
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8016..8948
                     /codon_start=1
                     /label=iRFP670
                     /note="color: #993366"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             8949..9014
                     /codon_start=1
                     /label=CaaX
                     /note="color: #993366"
                     /translation="LEKMSKDGKKKKKKSKTKCVIM"
     misc_feature    9077..9665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             9775..10008
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
       61 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
      121 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
      181 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
      241 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
      301 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      361 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      421 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      481 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      541 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      601 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      661 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      721 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2101 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2641 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2701 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2761 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     2821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3121 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     3181 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     3241 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     3301 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     3361 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     3421 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     3481 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     3541 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     3601 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     3661 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     3721 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     3781 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     3841 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     3901 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     3961 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     4021 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     4081 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     4141 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     4201 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     4261 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     4321 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4381 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4441 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4501 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4561 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4621 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     4681 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     4741 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     4801 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4861 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4921 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4981 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     5041 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     5101 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     5161 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     5221 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5281 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5341 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     5401 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     5461 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     5521 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     5581 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     5641 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5701 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5761 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5821 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5881 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     5941 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6001 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6061 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6121 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6181 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6241 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6301 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggtgatc
     6361 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6421 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     6481 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     6541 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     6601 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     6661 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     6721 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6781 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6841 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6901 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6961 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7021 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7081 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7141 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     7201 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     7261 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     7321 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     7381 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga
     7441 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     7501 ggtttggcaa gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa
     7561 gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag
     7621 gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag
     7681 tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca
     7741 gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc
     7801 cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg
     7861 agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt
     7921 cgcccggggg ggatctggag ctctcgagaa ttctcacgcg tctgcaggat atcaagcttg
     7981 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggc gcgtaaggtc gatctcacct
     8041 cctgcgatcg cgagccgatc cacatccccg gcagcattca gccgtgcggc tgcctgctag
     8101 cctgcgacgc gcaggcggtg cggatcacgc gcattacgga aaatgccggc gcgttctttg
     8161 gacgcgaaac tccgcgggtc ggtgagctac tcgccgatta cttcggcgag accgaagccc
     8221 atgcgctgcg caacgcactg gcgcagtcct ccgatccaaa gcgaccggcg ctgatcttcg
     8281 gttggcgcga cggcctgacc ggccgcacct tcgacatctc actgcatcgc catgacggta
     8341 catcgatcat cgagttcgag cctgcggcgg ccgaacaggc cgacaatccg ctgcggctga
     8401 cgcggcagat catcgcgcgc accaaagaac tgaagtcgct cgaagagatg gccgcacggg
     8461 tgccgcgcta tctgcaggcg atgctcggct atcaccgcgt gatgttgtac cgcttcgcgg
     8521 acgacggctc cgggatggtg atcggcgagg cgaagcgcag cgacctcgag agctttctcg
     8581 gtcagcactt tccggcgtcg ctggtcccgc agcaggcgcg gctactgtac ttgaagaacg
     8641 cgatccgcgt ggtctcggat tcgcgcggca tcagcagccg gatcgtgccc gagcacgacg
     8701 cctccggcgc cgcgctcgat ctgtcgttcg cgcacctgcg cagcatctcg ccctgccatc
     8761 tcgaatttct gcggaacatg ggcgtcagcg cctcgatgtc gctgtcgatc atcattgacg
     8821 gcacgctatg gggattgatc atctgtcatc attacgagcc gcgtgccgtg ccgatggcgc
     8881 agcgcgtcgc ggccgaaatg ttcgccgact tcttatcgct gcacttcacc gccgcccacc
     8941 accaacgcct cgagaaaatg tccaaggatg gtaagaaaaa gaagaagaag tcaaaaacca
     9001 agtgtgttat catgtaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     9061 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     9121 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     9181 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     9241 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     9301 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9361 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9421 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     9481 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9541 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9601 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9661 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     9721 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9781 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9841 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9901 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9961 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcag
//
