LOCUS       pTriex4-FLAG-GDI-Nlobe83-GS-iFKBP-design1        5475 bp    DNA
            circular
ACCESSION   DNA_pTriex4
VERSION     DNA_pTriex4.0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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        enzymeMap="AgeI|accggt,BsrGI|tgtaca,BspEI|tccgga,NcoI|ccatgg,NdeI|catatg,EcoRI|gaattc,SmaI|cccggg,SalI|gtcgac,XhoI|ctcgag,BglII|agatct,HindIII|aagctt,BamHI|ggatcc,ClaI|atcgat,MfeI|caattg,NheI|gctagc,NotI|gcggccgc"
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        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
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DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|2106|2116||0|null|null|null|128,255,0|null
            VNTSEQSTYLE|2121|2125||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|2153|2158||0|null|null|null|255,255,0|null
            VNTSEQSTYLE|2975|2982||0|null|null|null|179,179,179|null
            VNTSEQSTYLE|3244|3249||0|null|null|null|76,76,76|null
            VNTSEQSTYLE|2117|2120||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|2126|2140||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|2968|2973||0|null|null|null|204,204,204|null
            VNTSEQSTYLE|2983|3243||0|null|null|null|255,111,207|null
            VNTSEQSTYLE|2159|2167||0|null|null|null|255,102,102|null
            VNTSEQSTYLE|2168|2967||0|null|null|null|255,204,102|null
FEATURES             Location/Qualifiers
     misc_feature    2117..2140
                     /label=FLAG
                     /vntifkey="21"
     misc_feature    2363..2377
                     /label=GSx6
                     /vntifkey="21"
     misc_feature    2387..2647
                     /label=iFKBP
                     /vntifkey="21"
     misc_feature    2168..2362
                     /label=GDI-Nlobe-83
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct
     1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg
     1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg
     1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct
     1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
     1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag
     1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
     2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
     2101 aaggagatat accatggact ataaggacga tgatgacaaa ggatctggat ccgaattcgc
     2161 cgccaccatg gctgagcagg agcccacagc tgagcagttg gcacagattg cggcagagaa
     2221 cgaggaggac gagcactcag tcaactataa gcccccggcc cagaagagca ttcaggagat
     2281 ccaggagctg gacaaggacg acgagagtct gcgcaagtac aaggaggccc ttctgggccg
     2341 tgtggctgtg tctgctgacc ccggtggatc aggtggcgcg gccgcgacct gcgtggtgca
     2401 ctacaccggg atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc
     2461 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca
     2521 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac
     2581 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa
     2641 actggaatga ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg
     2701 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     2761 tgagatcgat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     2821 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2881 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2941 tttggtttag agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc
     3001 taaacgggtc ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt
     3061 ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt
     3121 attaaaacac gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt
     3181 gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt
     3241 ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta
     3301 aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg
     3361 aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt
     3421 gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga
     3481 cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt
     3541 gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt
     3601 tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg
     3661 attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat
     3721 ttgtagttga gctttttgga attgcgatcg cataacttcg tatagcatac attatacgaa
     3781 gttataagct cggaacgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3961 ggctccgccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4021 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4081 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4141 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4201 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4261 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4321 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4381 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4441 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4501 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgttaccaat
     4561 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4621 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4681 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4741 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4801 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4861 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4921 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4981 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5041 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5101 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5161 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5221 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5281 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5341 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5401 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5461 tcatgtccgc gcgtt
//
