LOCUS       pTriex4-FLAG-ITSNNlobe(Ins6)-GSreal-iFKBP        6528 bp    DNA
            circular
ACCESSION   DNA_pTriex4
VERSION     DNA_pTriex4.0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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        enzymeMap="AgeI|accggt,BsrGI|tgtaca,BspEI|tccgga,NcoI|ccatgg,NdeI|catatg,EcoRI|gaattc,SmaI|cccggg,SalI|gtcgac,XhoI|ctcgag,BglII|agatct,HindIII|aagctt,BamHI|ggatcc,ClaI|atcgat,MfeI|caattg,NheI|gctagc,NotI|gcggccgc"
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        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
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DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|2106|2116||0|null|null|null|128,255,0|null
            VNTSEQSTYLE|2121|2125||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|2153|2158||0|null|null|null|255,255,0|null
            VNTSEQSTYLE|3680|3685||0|null|null|null|76,76,76|null
            VNTSEQSTYLE|2117|2120||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|2126|2140||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|3419|3679||0|null|null|null|255,111,207|null
            VNTSEQSTYLE|2159|2167||0|null|null|null|255,102,102|null
            VNTSEQSTYLE|2168|2875||0|null|null|null|255,102,102|null
            VNTSEQSTYLE|2885|3394||0|null|null|null|255,204,102|null
FEATURES             Location/Qualifiers
     misc_feature    2117..2140
                     /label=FLAG
                     /vntifkey="21"
     misc_feature    3440..3700
                     /label=iFKBP
                     /vntifkey="21"
     misc_feature    2168..2875
                     /label=mCherry
                     /vntifkey="21"
     misc_feature    2885..3394
                     /label=ITSN-Nlobe(Ins6)
                     /vntifkey="21"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct
     1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg
     1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg
     1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct
     1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
     1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag
     1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
     2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
     2101 aaggagatat accatggact ataaggacga tgatgacaaa ggatctggat ccgaattcgc
     2161 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     2221 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     2281 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     2341 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     2401 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     2461 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     2521 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     2581 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     2641 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     2701 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2761 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2821 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg
     2881 aatggatatg ttgaccccaa ctgaaagaaa gcgacaagga tacatccacg agctcattgt
     2941 caccgaggag aactatgtga atgacctgca gctggtcaca gagatttttc aaaaacccct
     3001 gatggagtct gagctgctga cagaaaaaga ggttgctatg atttttgtga actggaagga
     3061 gctgattatg tgtaatatca aactactaaa agcgctgaga gtccgcaaga agatgtccgg
     3121 ggagaagatg cctgtgaaga tgattggaga catcctgagc gcacagctgc cgcacatgca
     3181 gccctacatc cgcttctgca gccgccagct caacggggct gccctgatcc agcagaagac
     3241 ggacgaggcc ccagacttca aggagttcgt caaaagattg gcaatggatc ctcggtgtaa
     3301 agggatgcca ctctctagtt ttatactgaa gcctatgcaa cgggtaacaa gatacccact
     3361 gatcattaaa aatatcctgg aaaacacccc tgaacattct gcaggtggat caggtggcgc
     3421 ggccgcgagc ggaggttcga cctgcgtggt gcactacacc gggatgcttg aagatggaaa
     3481 gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga
     3541 ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact
     3601 gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca
     3661 tgccactctc gtcttcgatg tggagcttct aaaactggaa tgactcgagc accaccatca
     3721 ccatcaccat cactaagtga ttaacctcag gtgcaggctg cctatcagaa ggtggtggct
     3781 ggtgtggcca atgccctggc tcacaaatac cactgagatc gatctttttc cctctgccaa
     3841 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     3901 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3961 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     4021 ccatatgtaa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     4081 gaaagcatgc ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg
     4141 caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt
     4201 acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt
     4261 ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat
     4321 tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt
     4381 ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat
     4441 tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat
     4501 tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg
     4561 tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaatagag
     4621 cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc
     4681 aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat
     4741 taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattgcga
     4801 tcgcataact tcgtatagca tacattatac gaagttataa gctcggaacg ctgcgctcgg
     4861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg ccccctgacg agcatcacaa
     5041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     5221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5581 aaaaggatct caagaagatc ctttgttacc aatgcttaat cagtgaggca cctatctcag
     5641 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5701 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5761 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5821 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5881 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5941 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6001 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6061 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6121 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6181 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6241 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6301 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6361 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6421 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6481 aatattattg aagcatttat cagggttatt gtctcatgtc cgcgcgtt
//
