LOCUS       Exported                8703 bp ds-DNA     circular SYN 20-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAC-U63-QtgRNA2.1
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Cornell University
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 20, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    234..263
                     /label=MCS
     promoter        264..681
                     /label=U6:3 promoter
                     /note="U6:3 promoter"
     misc_feature    707..802
                     /label=gRNA core 2.1
     misc_feature    803..918
                     /label=U6 genes 3' flanking sequence
                     /note="U6 genes 3' flanking sequence"
     CDS             join(1413..1484,1879..2152,2227..2881,2943..3258,3479..3610,
                     3681..4295)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    5073..5658
                     /label=P element 5' end
     misc_feature    5659..5886
                     /label=w linker
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(5893..6481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6652..7512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7513..7617)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    8092..8161
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    8202..8703
                     /label=w linker
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc gcaaggaaga gcgaggctct tcttgtgttt aagagctatg
      721 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaactttg ctggaaacag
      781 caaagtggca ccgagtcggt gctttttttg cctacctgga gcctgagagt tgttcaataa
      841 aataaaaatg tttcgttttt ttgctttcgc cagtatttat tatttttcat caatatgtat
      901 tcaatttggt atgtatttac tagtggccta attctagtat gtatgtaagt taataaaacc
      961 catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt
     1021 gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc
     1081 tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt
     1141 ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac
     1201 cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg
     1261 tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc
     1321 tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg
     1381 gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa
     1441 ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg
     1501 catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg
     1561 gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa atagtgacac
     1621 gaaacagatt attctggtag ctgtgctcgc tatataagac aatttttaag atcatatcat
     1681 gatcaagaca tctaaaggca ttcattttcg actacattct tttttacaaa aaatataaca
     1741 accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt
     1801 cgtaggatac ttcgttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     1861 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     1921 ttcgggcagg ccaaaaacta cggcacgctc ctgccaccca gtccgccgga ggactccggt
     1981 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     2041 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     2101 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     2161 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     2221 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     2281 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     2341 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     2401 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     2461 tgattttcca ggccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     2521 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     2581 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     2641 aggcactaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcat
     2701 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     2761 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     2821 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     2881 agtgagttcg atgtgtttat taagggtatc tagcattaca ttacatctca actcctatcc
     2941 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     3001 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     3061 tttgctatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatttggag
     3121 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     3181 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     3241 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     3301 tcttggaaac aaattcgcta gattttagtt agaattgcct gattccacac ccttcttagt
     3361 ttttttcaat gagatgtata gtttatagtt ttgcagaaaa taaataaatt tcatttaact
     3421 cgcgaacatg ttgaagatat gaatattaat gagatgcgag taacatttta atttgcagat
     3481 ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggcgtgat
     3541 gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc
     3601 cacgataaat gtaagtcttg tttagaatac atttgcatat taataattta ctaactttct
     3661 aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc
     3721 gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattaccgc
     3781 tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg
     3841 ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa
     3901 cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg
     3961 gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg
     4021 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
     4081 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
     4141 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
     4201 tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
     4261 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
     4321 tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     4381 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     4441 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
     4501 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
     4561 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
     4621 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
     4681 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt
     4741 ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc
     4801 caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt
     4861 tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt
     4921 gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg
     4981 ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat
     5041 gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta
     5101 ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct
     5161 aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa acttactctg
     5221 tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt
     5281 tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg
     5341 cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc
     5401 tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta
     5461 attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca
     5521 cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag
     5581 caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc
     5641 ttatgttatt tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa
     5701 gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca
     5761 gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta
     5821 acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa
     5881 gaagaaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6061 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     6121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6301 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7261 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7621 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7681 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     7741 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7801 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     7861 tgcggcatca gagcagattg tactgagagt gcaccatatc gacatgcccg ccgtgaccgt
     7921 cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg cacgtcccgt
     7981 gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg tgcgccgcgc
     8041 gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc agatgggtga
     8101 ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga
     8161 gaccgtgacc tacatcgtcg atatgcggtg tgaaataccg caccgaatcg cgcggaacta
     8221 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     8281 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     8341 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     8401 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     8461 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     8521 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
     8581 ataatggtaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
     8641 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
     8701 gac
//
