LOCUS       Exported                8516 bp ds-DNA     circular SYN 21-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pT2_CMV-eGFP.PB_PTK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 2, 2021 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(687..913)
                     /label=LIR
                     /note="Oriinal location: complement(687..913)"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        1045..1633
                     /label=CMV
                     /note="Oriinal location: 1045..1633"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1650..1950
                     /label=Delta5' eGFP
                     /note="Oriinal location: 1650..1950"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1951..2264
                     /label=5' piggyBac repeat
                     /note="Oriinal location: 1951..2264"
                     /note="5' piggyBac repeat"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2389..2904
                     /label=PGK promoter
                     /note="Oriinal location: 2389..2904"
                     /note="PGK promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2975..4576
                     /label=puroDeltaTK
                     /note="Oriinal location: 2975..4576"
                     /note="puroDeltaTK"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4577..4828
                     /label=bGHpA
                     /note="Oriinal location: 4577..4828"
                     /note="bGHpA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4944..5185
                     /label=3' piggyBac repeat
                     /note="Oriinal location: 4944..5185"
                     /note="3' piggyBac repeat"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5186..5601
                     /label=Delta3' eGFP
                     /note="Oriinal location: 5186..5601"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5635..5849
                     /label=pA
                     /note="Oriinal location: 5635..5849"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5947..6174
                     /label=RIR
                     /note="Oriinal location: 5947..6174"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(7531..8388)
                     /label=Amp
                     /note="Oriinal location: complement(7531..8388)"
                     /note="Amp"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgttcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
      721 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
      781 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
      841 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
      901 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
      961 atggctttag aagcttggta ccgcggccgc tctagagttt aaaccctagg atcgatgaat
     1021 tcgtttaaac gtcgacggcg cgcctagtta ttaatagtaa tcaattacgg ggtcattagt
     1081 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1381 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1441 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1501 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1561 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     1621 gaaccgtcag atcggcgcgc cgcggccgca tggtgagcaa gggcgaggag ctgttcaccg
     1681 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1741 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1801 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1861 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1921 aaggctacgt ccaggagcgc accatcttct ttaaccctag aaagatagtc tgcgtaaaat
     1981 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
     2041 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt
     2101 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
     2161 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
     2221 acgtgataac ttattatata tatattttct tgttatagat atcaactaga atgctagcac
     2281 aagtttgtac aaaaaagcag gctggcgccg gaaccaattc agtcgactgg atccggtacc
     2341 gggccccccc tcgaggtcga gacggtatcg ataagcttga tatcgaataa ttctaccggg
     2401 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     2461 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     2521 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     2581 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     2641 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     2701 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     2761 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     2821 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     2881 catctccggg cctttcgacc tgcagcctgt tgacaattaa tcatcggcat agtatatcgg
     2941 catagtataa tacgacaagg tgaggaacta aaccatgggg accgagtaca agcccacggt
     3001 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     3061 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     3121 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3181 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3241 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3301 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3361 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3421 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3481 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3541 gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcc atgcccacgc tactgcgggt
     3601 ttatatagac ggtcctcacg ggatggggaa aaccaccacc acgcaactgc tggtggccct
     3661 gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc aggtgctggg
     3721 ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat
     3781 atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta
     3841 tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc
     3901 cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta
     3961 cccggccgcg cgatacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc
     4021 cctcatcccg ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc cggaggacag
     4081 acacatcgac cgcctggcca aacgccagcg ccccggcgag cggcttgacc tggctatgct
     4141 ggccgcgatt cgccgcgttt acgggctgct tgccaatacg gtgcggtatc tgcagggcgg
     4201 cgggtcgtgg cgggaggatt ggggacagct ttcggggacg gccgtgccgc cccagggtgc
     4261 cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt
     4321 tcgggccccc gagttgctgg cccccaacgg cgacctgtac aacgtgtttg cctgggcctt
     4381 ggacgtcttg gccaaacgcc tccgtcccat gcacgtcttt atcctggatt acgaccaatc
     4441 gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt
     4501 caccaccccc ggctccatac cgacgatctg cgacctggcg cgcacgtttg cccgggagat
     4561 gggggaggct aactgagctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     4621 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4681 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4741 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4801 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     4861 agatccacta gttctagcct cgaggctaga gcggcccact cgagatatct agacccagct
     4921 ttcttgtaca aagtggtact agttaaaagt tttgttactt tatagaagaa attttgagtt
     4981 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     5041 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     5101 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     5161 aatatgatta tctttctagg gttaaggacg acggcaacta caagacccgc gccgaggtga
     5221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5281 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5341 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5461 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5521 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5581 tggacgagct gtacaagtaa gcggccgctt aattaagttt aaacccgctg atcagcctcg
     5641 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5701 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5761 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5821 tgggaagaca atagcaggca tgctggggat taattaagaa ttcaccggta ctagtagctt
     5881 gtggaaggct actcgaaatg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa
     5941 tactaattga gtgtatgtaa acttctgacc cactgggaat gtgatgaaag aaataaaagc
     6001 tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa ataaagtggt
     6061 gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg aattgtgaaa
     6121 aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca actgtatagg
     6181 gatcctctgg cccagcaggc cccagaagac cattaattaa ggccgggccg ctctagaact
     6241 agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
     6301 ggggggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     6361 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7201 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
