LOCUS       Exported               10289 bp ds-DNA     circular SYN 13-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lentiGuide-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 2, 2021 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..1264
                     /label=EF-1a
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1271..1984
                     /label=mCherry
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1999..2587
                     /label=WPRE
                     /note="color: #a6acb3; direction: RIGHT"
     LTR             2658..2893
                     /label=3' LTR SIN
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    2970..3100
                     /label=SV40 polyA
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3447..3877
                     /label=f1 Ori
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4248..5054
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(5180..5795)
                     /label=pUC Ori
                     /note="color: #a6acb3; direction: LEFT"
     LTR             6143..6555
                     /label=RSV/5'LTR
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    6606..6743
                     /label=psi
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6710..7074
                     /label=gag
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7220..7461
                     /label=RRE
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        7859..8103
                     /label=Human U6 Promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    8104..9988
                     /label=Filler (digest with BsmBI to replace filler wit...
                     /label=Filler (digest with BsmBI to replace filler 
                     withsgRNA oligos)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9989..10070
                     /label=chimeric RNA backbone 1 (BsmBI)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    10120..10237
                     /label=cPPT
                     /note="color: #a6acb3; direction: RIGHT"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgacgtacg atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     1321 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     1381 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     1441 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     1501 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     1561 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1621 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1681 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     1741 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1801 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1861 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1921 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa
     1981 ttaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2401 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2521 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2581 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2641 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2881 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     2941 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3121 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3181 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3241 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3301 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3901 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6061 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6121 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6181 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6241 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6301 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6361 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6421 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6481 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6541 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6601 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6661 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6721 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6781 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6841 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6901 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6961 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7021 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7081 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7141 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7201 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7261 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7321 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7381 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7441 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7501 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7561 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7621 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7681 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7741 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7801 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga
     7861 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     7921 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     7981 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     8041 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     8101 aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     8161 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     8221 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     8281 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     8341 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     8401 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     8461 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     8521 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     8581 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     8641 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     8701 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     8761 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     8821 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     8881 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     8941 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     9001 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     9061 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     9121 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     9181 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     9241 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     9301 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     9361 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     9421 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     9481 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     9541 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     9601 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     9661 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     9721 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     9781 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     9841 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     9901 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     9961 agcttcttgc tcttttttgt acgtctctgt tttagagcta gaaatagcaa gttaaaataa
    10021 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg
    10081 taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    10141 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    10201 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    10261 agatccactt tggcgccggc tcgaggggg
//
