LOCUS       Exported                9046 bp ds-DNA     circular SYN 13-NOV-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lentiGuide-GFP for5'-knock-in
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 2, 2021 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..413
                     /label=RSV/5'LTR
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    464..601
                     /label=psi
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    568..932
                     /label=gag
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1078..1319
                     /label=RRE
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1717..1961
                     /label=Human U6 Promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1966..3846
                     /label=Filler (digest with BsmBI to replace filler wit...
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3847..3928
                     /label=chimeric RNA backbone 1 (BsmBI)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3978..4095
                     /label=cPPT
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4156..4872
                     /label=eGFP
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4873..4890
                     /label=Linker
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4903..5491
                     /label=WPRE
                     /note="color: #a6acb3; direction: RIGHT"
     LTR             5562..5797
                     /label=3' LTR SIN
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    5874..6004
                     /label=SV40 polyA
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6351..6781
                     /label=f1 Ori
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7152..7958
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(8084..8699)
                     /label=pUC Ori
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cggttgtaaa
     1981 tgagcacaca aaatacacat gctaaaatat tatattctat gacctttata aaatcaacca
     2041 aaatcttctt tttaataact ttagtatcaa taattagaat ttttatgttc ctttttgcaa
     2101 acttttaata aaaatgagca aaataaaaaa acgctagttt tagtaactcg cgttgttttc
     2161 ttcaccttta ataatagcta ctccaccact tgttcctaag cggtcagctc ctgcttcaat
     2221 cattttttga gcatcttcaa atgttctaac tccaccagct gctttaacta aagcattgtc
     2281 tttaacaact gacttcatta gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc
     2341 tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct atttcacaag ctttcatgat
     2401 ttcttctttt gttaataaac aattttccat aatacattta acaacatgtg atccagctgc
     2461 tttttttaca gctttcatgt cttctaaaac taattcataa tttttgtctt ttaatgcacc
     2521 aatatttaat accatatcaa tttctgttgc accatcttta attgcttcag aaacttcgaa
     2581 tgcttttgta gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac
     2641 atttgtgcct tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt
     2701 tgcaaaatca aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc
     2761 ttgttttaat aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc
     2821 atttttaatt ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat
     2881 tcaccaacac ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca
     2941 aatcatgact tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt
     3001 ccatcgatgt agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt
     3061 gctctcaatg atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc
     3121 aagtttaaag tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct
     3181 actaatgttt tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct
     3241 tgtattccat ctttatctct agctgagtca tcaattacat atccataact ttcttcataa
     3301 gcaaaaacaa aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta
     3361 aatccagtta aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc
     3421 aaatcacttg ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt
     3481 tttagatttg ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt
     3541 acaaaatgac catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata
     3601 ataataatat ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca
     3661 aattctggat ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca
     3721 acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt
     3781 ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtac
     3841 gtctctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     3901 aaaagtggca ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg
     3961 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     4021 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     4081 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     4141 gagggggcca atgcgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4201 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4261 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4321 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4381 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4441 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4501 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4561 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4621 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4681 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4741 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4801 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4861 gagctgtaca agatcagatc tcgagccacc ggttaagtcg acaatcaacc tctggattac
     4921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5281 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5461 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5521 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5581 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6001 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6061 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6121 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6361 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6421 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6481 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6541 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6601 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6661 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6721 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6781 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9001 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
