LOCUS       Exported                7914 bp ds-DNA     circular SYN 13-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lentiGuide.LMNA01_LHA-GFP5'KI-RHA minus sgRNA cut sites
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 2, 2021 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    join(1..7914,1..0)
                     /note="color: #a6acb3; direction: RIGHT"
     LTR             1..413
                     /label=RSV/5'LTR
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    464..601
                     /label=psi
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    568..932
                     /label=gag
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1078..1319
                     /label=RRE
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1717..1961
                     /label=Human U6 Promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1966..1986
                     /label=LMNA sgRNA01
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1987..2068
                     /label=chimeric RNA backbone 1 (BsmBI)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2118..2235
                     /label=cPPT
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2296..2663
                     /label=LMNA LHA
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2664..3380
                     /label=eGFP
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3381..3398
                     /label=Linker
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3771..4359
                     /label=WPRE
                     /note="color: #a6acb3; direction: RIGHT"
     LTR             4430..4665
                     /label=3' LTR SIN
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    4742..4872
                     /label=SV40 polyA
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5219..5649
                     /label=f1 Ori
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(6952..7567)
                     /label=pUC Ori
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgccat ggagaccccg
     1981 tcccaggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     2041 aaaagtggca ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg
     2101 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2221 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     2281 gagggggcca atgcggggcc cgactccgcc acaccccaac ggtccttccc cctcctcacc
     2341 actcccgccc ccacccccaa tggatctggg actgcccctt taagagtagt ggcccctcct
     2401 cccttcagag gaggacctat tagagccttt gccccggcgt cggtgactca gtgttcgcgg
     2461 gagcgccgca cctacaccag ccaacccaga tcccgaggtc cgacagcgcc cggcccagat
     2521 ccccacgcct gccaggagca agccgagagc cagccggccg gcgcactccg actccgagca
     2581 gtctctgtcc ttcgacccga gccccgcgcc ctttccggga cccctgcccc gcgggcagcg
     2641 ctgccaacct gccggccatg gagatggtga gcaagggcga ggagctgttc accggggtgg
     2701 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2761 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2821 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2881 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2941 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3001 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3061 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3121 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3181 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3241 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3301 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3361 gcatggacga gctgtacaag atcagatctc gagccaccac cccgtctcag cggcgcgcca
     3421 cccgcagcgg ggcgcaggcc agctccactc cgctgtcgcc cacccgcatc acccggctgc
     3481 aggagaagga ggacctgcag gagctcaatg atcgcttggc ggtctacatc gaccgtgtgc
     3541 gctcgctgga aacggagaac gcagggctgc gccttcgcat caccgagtct gaagaggtgg
     3601 tcagccgcga ggtgtccggc atcaaggccg cctacgaggc cgagctcggg gatgcccgca
     3661 agacccttga ctcagtagcc aaggagcgcg cccgcctgca gctggagctg agcaaagtgc
     3721 gtgaggagtt taaggagctg aaagcgcggt gagttcgccc ttaagtcgac aatcaacctc
     3781 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3841 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3901 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3961 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4021 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4081 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4141 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4201 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4261 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4321 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     4381 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4441 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4501 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4561 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4621 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     4681 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4741 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4861 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5041 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5101 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5161 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5221 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5281 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5341 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5401 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5461 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5521 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5581 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5641 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5701 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5821 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5881 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5941 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6001 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6061 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6121 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6181 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6241 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6301 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6361 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6421 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6481 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6541 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6601 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6661 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6721 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6781 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7021 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7081 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7141 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7201 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7261 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7321 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7441 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7501 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7561 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7621 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7681 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7741 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7801 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7861 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
