LOCUS       Exported               13803 bp ds-DNA     circular SYN 14-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13803)
  AUTHORS   Ben Major WUSTL
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 20, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13803
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             131..991
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2074..2104
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     3987..4006
                     /label=DK58. p663-667-CSP-Fwd2
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4190
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4190..5401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    complement(5405..5425)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5426..5435
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5438..6208
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6215..6256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6257..6281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6265..6269
                     /label=BsrGI-dual Cutsite
                     /note="For cutting in the insert flanking attB, L, P sites 
                     (and the cddB gene)"
     misc_feature    6282..6283
                     /label=NN Fill for N-term tagging
     misc_feature    6284..6286
                     /label=HvdLib Fill
     CDS             6287..10624
                     /codon_start=1
                     /label=DK098. PIK3C2G
                     /translation="MAYSWQTDPNPNESHEKQYEHQEFLFVNQPHSSSQVSLGFDQIVD
                     EISGKIPHYESEIDENTFFVPTAPKWDSTGHSLNEAHQISLNEFTSKSRELSWHQVSKA
                     PAIGFSPSVLPKPQNTNKECSWGSPIGKHHGADDSRFSILAPSFTSLDKINLEKELENE
                     NHNYHIGFESSIPPTNSSFSSDFMPKEENKRSGHVNIVEPSLMLLKGSLQPGMWESTWQ
                     KNIESIGCSIQLVEVPQSSNTSLASFCNKVKKIRERYHAADVNFNSGKIWSTTTAFPYQ
                     LFSKTKFNIHIFIDNSTQPLHFMPCANYLVKDLIAEILHFCTNDQLLPKDHILSVCGSE
                     EFLQNDHCLGSHKMFQKDKSVIQLHLQKSREAPGKLSRKHEEDHSQFYLNQLLEFMHIW
                     KVSRQCLLTLIRKYDFHLKYLLKTQENVYNIIEEVKKICSVLGCVETKQITDAVNELSL
                     ILQRKGENFYQSSETSAKGLIEKVTTELSTSIYQLINVYCNSFYADFQPVNVPRCTSYL
                     NPGLPSHLSFTVYAAHNIPETWVHRINFPLEIKSLPRESMLTVKLFGIACATNNANLLA
                     WTCLPLFPKEKSILGSMLFSMTLQSEPPVEMITPGVWDVSQPSPVTLQIDFPATGWEYM
                     KPDSEENRSNLEEPLKECIKHIARLSQKQTPLLLSEEKKRYLWFYRFYCNNENCSLPLV
                     LGSAPGWDERTVSEMHTILRRWTFSQPLEALGLLTSSFPDQEIRKVAVQQLDNLLNDEL
                     LEYLPQLVQAVKFEWNLESPLVQLLLHRSLQSIQVAHRLYWLLKNAENEAYFKSWYQKL
                     LAALQFCAGKALNDEFSKEQKLIKILGDIGERVKSASDHQRQEVLKKEIGRLEEFFQDV
                     NTCHLPLNPALCIKGIDHDACSYFTSNALPLKITFINANPMGKNISIIFKAGDDLRQDM
                     LVLQLIQVMDNIWLQEGLDMQMIIYRCLSTGKDQGLVQMVPDAVTLAKIHRHSGLIGPL
                     KENTIKKWFSQHNHLKADYEKALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMF
                     HIDFGKFLGHAQTFGGIKRDRAPFIFTSEMEYFITEGGKNPQHFQDFVELCCRAYNIIR
                     KHSQLLLNLLEMMLYAGLPELSGIQDLKYVYNNLRPQDTDLEATSHFTKKIKESLECFP
                     VKLNNLIHTLAQMSAISPAKSTSQTFPQESCLLSTTRSIERATILGFSKKSSNLYLIQV
                     THSNNETSLTEKSFEQFSKLHSQLQKQFASLTLPEFPHWWHLPFTNSDHRRFRDLNHYM
                     EQILNVSHEVTNSDCVLSFFLSEAVQQTVEESSPVYLGEKFPDKKPKVQLVISYEDVKL
                     TILVKHMKNIHLPDGSAPSAHVEFYLLPYPSEVRRRKTKSVPKCTDPTYNEIVVYDEVT
                     ELQGHVLMLIVKSKTVFVGAINIRLCSVPLDKEKWYPLGNSII"
     CDS             6671..6673
                     /codon_start=1
                     /product="Sequence conflicti129Missing in EAW96387 (Ref. 3)
                     
                     Sequence conflicti129Missing in AAI30278 (PubMed:15489334) 
                     "
                     /label=Missing 129P (385-387 bp)
                     /translation="P"
     CDS             6722..6724
                     /codon_start=1
                     /product="Natural varianti VAR_056676146P --> L3 
                     Corresponds to variant dbSNP:rs11044004 "
                     /label=C437>T (P146-5L) mutation
                     /translation="P"
     CDS             10454..10456
                     /codon_start=1
                     /product="C4170>A (P1390P) silent mutation"
                     /label=C4170>A (P1390P) silent mutation
                     /translation="P"
     misc_feature    10625
                     /label=N Fill for C-term tagging
     protein_bind    complement(10627..10651)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10681..11269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10734..10754)
                     /label=wPRE-R SeqRevPrimer
     primer_bind     complement(10794..10813)
                     /label=p663-667-CSP-Rev2
     CDS             complement(11152..11163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11283..11782
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             11803..12402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             12540..12773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12845..12966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      12985..13141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    13007..13027
                     /label=SV40 ori mutation
                     /note="SV40 origin of replication mutation compared to the 
                     original sequence"
     promoter        complement(13162..13180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13190..13206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13199..13221)
                     /label=SeqFwd250s Primer
     rep_origin      13348..13803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc
     4201 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     4261 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     4321 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     4381 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     4441 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     4501 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     4561 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     4621 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     4681 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     4741 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     4801 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     4861 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     4921 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     4981 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     5041 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     5101 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     5161 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     5221 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     5281 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     5341 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     5401 cggccaactt ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca
     5461 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     5521 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     5581 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     5641 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5701 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5761 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5821 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5881 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5941 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     6001 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     6061 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     6121 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     6181 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
     6241 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatgg catattcttg
     6301 gcaaacggat ccaaatccta atgaatcaca cgaaaagcag tatgaacacc aagaatttct
     6361 ctttgtaaat caaccccatt cttctagcca agtcagtctg ggttttgatc agatagtaga
     6421 tgagatcagt ggcaaaattc cacactacga gagtgaaatt gatgaaaaca ccttttttgt
     6481 gcccactgca ccaaaatggg actcaacagg gcattcatta aatgaagcac accaaatatc
     6541 cttgaatgaa ttcacttcta aaagccgtga actctcctgg catcaagtta gcaaagcacc
     6601 agcaattggt tttagtcctt ctgtgttacc aaaacctcaa aatacgaata aagaatgctc
     6661 ctggggaagc cccataggaa aacatcatgg tgctgatgat tccagattca gtattttagc
     6721 tccatcattc acaagtttgg ataaaattaa tctagagaaa gaattagaaa atgaaaatca
     6781 taactaccat ataggatttg aaagtagcat tcctccaaca aattcatcct tctcaagtga
     6841 cttcatgccg aaagaagaga ataaaaggag tggacatgtg aacattgtgg aaccatcttt
     6901 gatgcttttg aaaggctctc ttcaacccgg aatgtgggaa agtacatggc agaagaatat
     6961 agagtcaata ggttgttcca ttcagctagt ggaagtacct caaagcagca atacgagtct
     7021 ggcctctttt tgcaacaaag taaaaaaaat cagagaaaga tatcatgcag ctgatgttaa
     7081 tttcaattct gggaagatct ggagcactac tacagcattt ccgtatcagc tcttttctaa
     7141 gaccaagttt aatatacata tttttattga taactcaaca caacctcttc attttatgcc
     7201 atgtgctaat tatcttgtca aagatctaat tgcagaaatt ctgcattttt gcacaaatga
     7261 ccagctactc cccaaagatc atattctaag tgtatgtggc tctgaagaat ttttacaaaa
     7321 cgaccactgt ttggggagcc acaaaatgtt tcaaaaagat aaatctgtta ttcagctcca
     7381 cctgcagaaa agtagggaag ctccaggaaa gctatctcga aagcatgaag aggaccacag
     7441 tcagttttat ctgaatcaac ttctagaatt tatgcatatt tggaaagtat ccagacaatg
     7501 tctcttaaca ctcatcagaa aatatgactt ccacctgaaa tacctattga aaacccagga
     7561 aaacgtgtat aatattattg aagaagttaa aaaaatatgc agtgttctag ggtgtgtgga
     7621 aaccaaacaa attacagatg cagtaaatga actaagtcta attcttcaga gaaaaggaga
     7681 gaatttttat caaagttcag agacttcagc aaaaggcttg atagagaagg taacaactga
     7741 actatccaca tccatctacc agctaatcaa tgtctactgt aacagctttt atgcagattt
     7801 tcagcctgta aatgtaccta gatgcacttc ctatctaaat cccgggcttc cttcccacct
     7861 cagcttcaca gtgtatgcag cacacaacat tccagaaacc tgggtgcaca ggatcaattt
     7921 tccccttgaa ataaagtcac ttccaaggga atccatgctc actgtaaaac tgtttgggat
     7981 tgcctgtgca accaacaatg caaatttact ggcgtggact tgtcttccac tgtttccaaa
     8041 agaaaaatcc attctcgggt ctatgctgtt cagcatgaca ttacagagtg agcctcccgt
     8101 agaaatgata actccaggag tgtgggatgt aagtcagcca tccccggtga ccctgcagat
     8161 tgattttcca gctactgggt gggagtatat gaaacctgat tctgaagaga atagaagtaa
     8221 tcttgaagag ccactaaagg agtgtataaa acatattgcc agactttcac agaaacagac
     8281 tcccctacta ctctctgaag aaaagaaaag atatttatgg ttttatcgct tctactgcaa
     8341 taatgaaaac tgctcccttc ctttagtcct gggtagtgcc cctggatggg atgaaaggac
     8401 tgtttcagaa atgcatacca ttttgagaag atggacattt tctcaacctt tagaggctct
     8461 tgggcttttg acttccagtt ttccagatca agaaattcgt aaagtggcag ttcaacaatt
     8521 agacaacctc ttgaatgatg aactactgga atatctccca cagctagttc aggctgtcaa
     8581 gtttgaatgg aaccttgaga gtcctttagt gcaacttcta ctccaccgct ccttgcagag
     8641 catccaggtt gcccatcgtc tttactggct gctaaaaaat gcagaaaatg aagcttattt
     8701 taaaagctgg tatcagaagc tactagctgc tctccaattc tgtgcaggta aagccttgaa
     8761 tgatgagttt tccaaggagc agaaacttat caaaattctg ggagatattg gggaaagagt
     8821 caagtctgcc agtgaccatc aaagacagga ggtactgaag aaagaaattg gcagactaga
     8881 agagttcttt caagatgtaa atacttgtca tcttcctctg aaccctgccc tatgtataaa
     8941 agggattgat cacgatgcat gttcatattt tacatctaat gctttgccat tgaagattac
     9001 tttcatcaat gctaatccga tgggcaaaaa catcagcatt atttttaagg ctggagatga
     9061 tcttcgtcag gatatgcttg ttctgcagct tattcaagtg atggacaata tttggctgca
     9121 ggaaggcttg gatatgcaaa tgatcattta tagatgtcta tccacaggaa aagaccaagg
     9181 attggtgcag atggtacctg atgctgtgac cctagcaaag attcatcgcc attctggact
     9241 gataggacca ttgaaagaaa atacaattaa aaagtggttc agtcagcaca accacttaaa
     9301 ggcagattat gaaaaggcct tgaggaactt tttctactcc tgtgctggct ggtgtgtggt
     9361 aacattcatc ctgggagtat gtgaccgtca caatgataat atcatgctga caaagtcggg
     9421 ccacatgttt catattgact ttggaaaatt cttaggtcat gcacaaacat ttggagggat
     9481 aaaaagggac cgagctcctt tcatttttac ttcagagatg gaatacttta ttacagaggg
     9541 tgggaaaaac ccacagcatt ttcaagattt tgtggaactt tgctgtcgtg cttataatat
     9601 tatcagaaag cacagccaac tgctcttgaa cctgctggaa atgatgctgt atgcaggact
     9661 gcctgagcta agtggaattc aagacctgaa atatgtgtat aataatcttc gtccacaaga
     9721 cacagacctg gaagcaacaa gtcattttac caagaaaata aaggaaagtc tggagtgttt
     9781 ccctgttaaa ttgaataact tgatccacac acttgcacaa atgtcagcca taagccctgc
     9841 caaatctact tcacagactt ttcctcagga atcctgtttg ctgagtacaa ctaggtcgat
     9901 tgaaagagca acaattttag ggttcagcaa gaaatccagt aatctgtatc tgatccaggt
     9961 gacacacagc aacaacgaaa caagcctgac agaaaaatca tttgagcagt tttcaaaact
    10021 tcacagccaa cttcagaagc agtttgcatc actgactctc ccagagtttc ctcattggtg
    10081 gcacctacct tttacaaatt cagatcacag aagattcaga gatctaaatc attacatgga
    10141 acagatatta aatgtatcac atgaagttac aaacagtgat tgtgtactta gctttttcct
    10201 ctctgaggct gtgcaacaaa cagttgaaga atcatcacct gtgtacctag gtgagaagtt
    10261 tccagacaag aagcctaagg tgcagttagt catatcctac gaggatgtga agctgaccat
    10321 actagtgaaa cacatgaaaa acattcatct cccagatggc tctgcgccca gtgcacatgt
    10381 tgaattttat cttttaccat atcccagtga agttcgtagg aggaaaacaa aatctgttcc
    10441 aaaatgtacg gacccaactt acaatgaaat tgtagtatat gatgaagtca cagagctcca
    10501 aggacatgtc ttaatgctta ttgtgaagag taaaactgta tttgtgggag caattaacat
    10561 ccgactctgt agtgtcccac tcgataaaga aaaatggtat ccattaggaa acagtataat
    10621 ttagggaccc agctttcttg tacaaagtgg tgatatccag cacagtggcg gccgctcgac
    10681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    11041 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
    11101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    11221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
    11281 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
    11341 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
    11401 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
    11461 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
    11521 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
    11581 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
    11641 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
    11701 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
    11761 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
    11821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
    11881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
    11941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
    12001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
    12061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
    12121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
    12181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
    12241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
    12301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
    12361 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
    12421 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
    12481 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    12541 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
    12601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    12661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    12721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    12781 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    12841 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12961 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
    13021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    13081 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    13141 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    13201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    13261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    13321 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    13381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    13441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    13501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    13561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    13621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    13681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    13741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    13801 ttt
//
