LOCUS       Exported                7718 bp ds-DNA     circular SYN 17-APR-2021
DEFINITION  Bacterial vector with a ColA origin for the co-expression of two 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 20, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    417..1265
                     /label=rhaR
                     /note="DNA-binding transcriptional activator for rhaSR, 
                     L-rhamnose-binding"
     misc_feature    1339..2175
                     /label=rhaS
                     /note="DNA-binding transcriptional activator for rhaBAD and
                     RhaT,"
     misc_feature    2177..2439
                     /label=Prha
     RBS             2440..2445
     misc_feature    2451..5959
                     /label=tnsABC
     terminator      5983..6030
                     /label=T7 terminator
     CDS             complement(6260..7075)
                     /codon_start=1
                     /label=KanR
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(7076..7167)
                     /label=AmpR promoter
     rep_origin      complement(join(7185..7718,1..102))
                     /direction=LEFT
                     /label=ColA ori
ORIGIN
        1 cgatcaaacc gcctccccag gcggtttttt cgtttacaga gcaggagatt acgacgatcg
       61 taaaaggatc tcaagaagat cctttacgga ttcccgacac catcactcta gatttcagtg
      121 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
      181 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
      241 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcggg
      301 ttcttatggc tcttgtatct atcagtgaag catcaagact aacaaacaaa agtagaacaa
      361 ctgttcaccg ttacatatca aagggaaaac tgtccatacc catggggcat ggcgaattaa
      421 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
      481 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
      541 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgtcg cagatattga
      601 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
      661 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
      721 atcagcttat ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga
      781 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
      841 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
      901 cccatgctac ctaagcgcca gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc
      961 ccctgccagt caagattcag cttcagacgc tccgggcaat aaataatatt ctgcaaaacc
     1021 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca
     1081 cgggtaatgc gataagggcg atcgttgagt acatgcaggc cattaccgcg ccagacaatc
     1141 accagctcac aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc
     1201 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
     1261 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
     1321 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
     1381 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
     1441 gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat
     1501 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
     1561 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
     1621 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag
     1681 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
     1741 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
     1801 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
     1861 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
     1921 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
     1981 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
     2041 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
     2101 ctgcgggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact
     2161 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
     2221 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
     2281 atacagcgtg aattttcagg aaatgcggtg agcatcacat caccacaatt cagcaaattg
     2341 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
     2401 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa aggagatata ccatggcgac
     2461 aagtttacct acgccctcag caattacgac ttcggcgtta gagtatgcat tccatactcc
     2521 cgctcgcaat ctaacgaaat ctcgcggaaa aaacattcat cgttatgtca gtgtaaagat
     2581 gagtaagagg attacggtag aatctactct agagtgtgat gcctgctatc actttgattt
     2641 tgagccaagt attgttcgct tttgcgctca accgattcga tttttatatt atctcaatgg
     2701 tcagtctcac tcctatgttc ctgactttct agttcaattt gataccaacg agtttgttct
     2761 atatgaagta aagtcagctt atgctaagaa caaacctgat tttgatgttg aatgggaggc
     2821 gaaagtaaaa gcagcaactg aactagggct agaattggag cttgttgaag agagtgatat
     2881 tagggatacg gttgtattaa ataatcttaa gcgcatgcat cgttatgctt cgaaagatga
     2941 gctgaataac gtacataact ctctcttaaa aataataaag tacaatggcg cccaatctgc
     3001 aagatgcttg ggagaacagt tgggtttaaa aggccgaact gttttaccaa ttttgtgcga
     3061 tttgctgtca aggtgtttac tcgatacacg tttggataag cctctatctc ttgaatctcg
     3121 atttgagttg gccagttatg gctaagaaag ggttctcaag tttccataga aaagcagtct
     3181 cttcccaaga tacgcttgaa tcgatagagc ttgtctctag cgctaattgt ctagaaagtg
     3241 ttacgtatca agatatatca gcatttcccg aaacaattgc ggtagagatt aatttccgat
     3301 taagcattct tcgcttttta gcgcgaaagt gcgaaaccat tgtggccaaa tcaattgaac
     3361 cacatcgtgt agagctacag caaaactata gtagaaaaat acccagtgca ataacgatat
     3421 atcgatggtg gcttgctttt cgaaaatcag actacaaccc cattagctta gcacctaata
     3481 tcaaggatag aggtaataga gagacaaaag tgtcaacagt tgttgattct attatggaac
     3541 aggcagttga aagagttata tctggacgaa aagtcaatgt tagctctgca tataaacgtg
     3601 ttcgacgaaa agttcgtcaa tacaatctaa ctcatggaac gaaatacacg tatcctaagt
     3661 acgaatctgt aagaaagcga gtaaaaaaga aaaccccatt tgagttatta gccgcaggga
     3721 aaggggagag agtagctaag agagagtttc gccgaatggg aaaaaagatc ctcacgtcta
     3781 gcgtgctaga gagggttgaa atagatcaca ctgtcgttga cctttttgca gtacacgaag
     3841 agtatcgaat cccattgggc cgaccttggc ttactcaatt ggttgattgt tacagtaaag
     3901 ctgtaatcgg tttttattta ggtttcgagc ctcctagcta tgtgtcggtt tcccttgcac
     3961 ttaagaatgc aatacaacgc aaagatgact taatctcctc gtatgaatcg atcgagaatg
     4021 aatggctatg ttatggcatc ccagacctac tcgtaactga taatggtaaa gagtttttgt
     4081 cgaaagcatt tgatcaagca tgtgaatcac tattgatcaa tgtgcatcaa aataaagttg
     4141 agacgcccga caacaaacct catgttgaac gtaactacgg gactattaat acttctctgt
     4201 tagacgattt acctgggaaa tccttcagcc agtaccttca aagagaaggg tacgactctg
     4261 tgggagaagc tacccttaca ctcaatgaga ttagagaaat ttacttaatt tggttggtgg
     4321 atatttatca taaaaaaccc aatcagagag gcactaattg tcctaatgtt gcgtggaaaa
     4381 agggttgtca agaatgggaa ccagaggagt tctctggttc taaagacgaa ttagacttta
     4441 aatttgctat tgttgattac aaacaactta ctaaagtagg gataactgtc tacaaagaac
     4501 tgagttatag caatgaccgt ttagctgaat atagagggaa gaaaggaaac cataaagttc
     4561 agttcaagta taaccctgag tgtatggcag ttatttgggt gttggatgag gatatgaatg
     4621 agtactttac agttaatgcg attgactacg aatatgcaag tagagtatca ctttggcaac
     4681 ataaatataa catgaaatat caagcagaac taaattcagc agaatatgat gaggacaagg
     4741 aaattgatgc agaaataaaa attgaagaaa tcgcagatcg ttcaattgtt aagactaaca
     4801 aaatcagagc tcggaggcgt ggcgctaggc atcaagagaa tagcgcaagg gctaagtcaa
     4861 tcagtaatgc gaacccggcc tcgatacaaa aacatgaaga tgaaatcgtt agtgcagata
     4921 atgacgattg ggatattgat tatgtctgag aaatcgtcaa atgagtgaaa cgcgtgaggc
     4981 tcgtatatca agagctaaaa gggcatttgt atccacaccg agcgttagga aaatcttgag
     5041 ttacatggat agatgtagag atctatcaga cctagagtct gagcctacat gcatgatggt
     5101 ctatggtgct tcgggtgtag gtaaaacgac cgtcatcaag aaatacttaa atcaaaacag
     5161 aagagagtcc gaagccgggg gcgatataat accggttttg catattgagt tgccagacaa
     5221 tgcgaagcca gtagatgcag caagggaatt gctggttgaa atgggtgacc cgctagcact
     5281 ttatgaaact gacttagcta gattgacgaa aagactgact gaattaatcc ctgcggtcgg
     5341 cgtgaagctg attattatcg atgagttcca acatttggtg gaagaaaggt caaatcgggt
     5401 tcttacccaa gtaggtaatt ggctaaaaat gatacttaac aaaacgaaat gtccaattgt
     5461 tatatttggt atgccatact caaaagttgt actgcaagca aactcgcaac ttcacgggcg
     5521 attttccatt caggttgaac tgcgcccctt tagctaccag ggaggtagag gtgtatttaa
     5581 aacttttttg gaataccttg ataaagccct accttttgaa aaacaggctg gcttagccaa
     5641 cgaaagtttg cagaaaaaat tgtatgcatt ctctcaggga aacatgcgtt cgttgagaaa
     5701 ccttatttat caagcatcta tcgaagcaat tgataatcag catgagacga taaccgaaga
     5761 agatttcgtt tttgcatcga agttgacatc gggcgataaa cccaactcat ggaaaaatcc
     5821 ttttgaggag ggtgttgagg taacagaaga tatgttacga ccgccaccaa aagatattgg
     5881 ttgggaggac tatttgagac attcaacccc gagagtgagt aaaccaggta gaaataaaaa
     5941 ctttttcgaa taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     6001 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
     6061 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     6121 taacgaccct gccctgaacc gacgacaagc tgacgaccgg gtctccgcaa gtggcacttt
     6181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6241 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
     6301 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     6361 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     6421 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     6481 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     6541 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     6601 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcggtcgctg ttaaaaggac
     6661 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     6721 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     6781 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     6841 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     6901 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     6961 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     7021 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcatactct
     7081 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7141 ttgaatgtat ttagaaaaat aaacaaatag gcatgctagc gcagaaacgt cctagaagat
     7201 gccaggagga tacttagcag agagacaata aggccggagc gaagccgttt ttccataggc
     7261 tccgcccccc tgacgaacat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     7321 caggactata aagataccag gcgtttcccc ctgatggctc cctcttgcgc tctcctgttc
     7381 ccgtcctgcg gcgtccgtgt tgtggtggag gctttaccca aatcaccacg tcccgttccg
     7441 tgtagacagt tcgctccaag ctgggctgtg tgcaagaacc ccccgttcag cccgactgct
     7501 gcgccttatc cggtaactat catcttgagt ccaacccgga aagacacgac aaaacgccac
     7561 tggcagcagc cattggtaac tgagaattag tggatttaga tatcgagagt cttgaagtgg
     7621 tggcctaaca gaggctacac tgaaaggaca gtatttggta tctgcgctcc actaaagcca
     7681 gttaccaggt taagcagttc cccaactgac ttaacctt
//
