LOCUS       Exported                3431 bp ds-DNA     circular SYN 25-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   john
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 20, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        69..1195
                     /label=Chantico Promoter
     misc_feature    883..890
                     /label=TBX5 consensus binding site
     rep_origin      complement(1502..2090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1967..1986
                     /label=OriseqF
     CDS             complement(2290..3150)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2944..2963)
                     /label=pGL4seqR3
     polyA_signal    3255..3303
                     /note="synthetic polyadenylation signal"
     misc_feature    3317..3408
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    3317..3390
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3357..3376
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
       61 aggatccggt cattcagtaa atataaatgc agatagccta cgttctgaaa gtggggttct
      121 agggtccctt ggttagtttt aaaaattcaa agtcactttg agttttgttt tgttttgttt
      181 tgttttaaag cttgagagtg gccgtgcaga gcaaagttgt ccaattaagt ttatatcagt
      241 gacagatggg cttatcagac cttcgctctc agacagtagc catggaacac ttagctgctg
      301 cctcccgaaa gtgcaagcag accagttcag gatctgaagc tttgttttgg tttgacttaa
      361 atgtaagtgc aaacagtcgc tgtggggtgt gtgtgtgtgt gtgtgtgtga tactggccga
      421 tgcaggctga gagtaggggt gtctcactcc tgactgcgta ctccccctct gttttacaca
      481 ggcttattta ctgttacacg gagacttttc ttcgaactgt taaactcttg gctgagtttg
      541 tgtggagtat gaggcaaatc tctaaccgca agcatgcagt ggaaagatta ggcatacttt
      601 tgaaacgagt ttgtgtacca gatcatattc ttggctcaag aaagaaaaag aaagggggct
      661 ggggaaaggc ttgtggttgc tactctggga actaaagaat tcaaaaggct tgttctccat
      721 gtttcgtaga cacagacatc tggaattgtt gttttaaaat tacaagcaat tgtgtatgct
      781 ttgttactgc aacacttatt cagaggcaag gacataggtg ccactgttgt cagaaagcca
      841 gatgtccttt ccttaaattg gcctgcctgg ctgctggcgc ctgggtgtca gcagacactt
      901 tgggaatctc agttagcggt actgcatgga tatagtgcat ctgcttctca cacttctgcc
      961 aaccttgtgt attggtttgc ttctctccat ggtgttaaaa caatgactga aagcgtcttg
     1021 ggtagggcaa gggtatattt agcttatagt tgcacatgac agtccatcgt caagggagag
     1081 catggcggga actcaaggac tttgaggagg aactgaagca caggcataga ggctgttcct
     1141 cactggcttg ctccccaggg cttgctcagc tagctgtctt acagagccca gaaccccgat
     1201 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     1261 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     1321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2221 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     2281 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     2341 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     2401 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     2461 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     2521 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     2581 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     2641 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     2701 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     2761 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     2821 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     2881 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     2941 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     3001 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     3061 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     3121 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     3181 ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt
     3241 attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     3301 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     3361 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc
     3421 cggtacctga g
//
