LOCUS       Exported                6313 bp ds-DNA     circular SYN 21-APR-2021
DEFINITION  Vector for fusing Dendra2 to the N-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 21, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             603..653
                     /codon_start=1
                     /label=hCALR SP
                     /translation="MLLSVPLLLGLLGLAVA"
     CDS             654..2936
                     /codon_start=1
                     /label=mEmerald-ERGIC-53
                     /translation="APVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTI
                     FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQ
                     KNGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKSGDELYKGGGGSGGGGSGGGGSIDELNSRVRELTDPD
                     GVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQR
                     GSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGSADLWNGVGI
                     FFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYY
                     QNTLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQL
                     TEPGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESV
                     GDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQIT
                     QQELDTVVKTQHEILRQVNEMKNSLSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLH
                     IVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVHFIIFVVVQTVLFIGYIMYRSQQE
                     AAAKKFF"
     CDS             669..1385
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1449..2936
                     /codon_start=1
                     /label=ERGIC-53
                     /translation="SIDELNSRVRELTDPDGVGGDPAVALPHRRFEYKYSFKGPHLVQS
                     DGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIG
                     ADGLAIWYAENQGLEGPVFGSADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDH
                     QNDGASQALASCQRDFRNKPYPVRAKITYYQNTLTVMINNGFTPDKNDYEFCAKVENMI
                     IPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKEISEKEKEKYQEEFEHF
                     QQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMI
                     LDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSLSETV
                     RLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFP
                     SCLSTVHFIIFVVVQTVLFIGYIMYRSQQEAAAKKFF"
     misc_feature    2951..2977
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     polyA_signal    3101..3222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3229..3684)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3711..3815
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3817..4174
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4025..4160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4209..5003
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5235..5282
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5611..6199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgctgct atccgtgccg ttgctgctcg gcctcctcgg cctggccgtc gccgcaccgg
      661 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      721 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      781 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      841 ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc
      901 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      961 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1021 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1081 tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc
     1141 agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc
     1201 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1261 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     1321 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1381 acaagtccgg agacgagctg tacaagggtg gcggtggctc gggcggtggt gggtcgggtg
     1441 gcggcggatc tatcgatgaa ttgaattcac gcgttcgcga gttaacggat ccagacggcg
     1501 tgggaggaga ccccgcggtc gcgttgccac atcgccgttt cgagtacaaa tacagcttca
     1561 aggggccgca cctggtgcag agcgacggga ccgtgccctt ctgggcccac gcggggaatg
     1621 ctattccaag ttcagatcaa attcgagtag caccatcttt aaaaagccaa agaggctcag
     1681 tgtggacaaa gacaaaagcg gcctttgaga actgggaagt tgaggtgaca tttcgagtga
     1741 ctggaagagg tcgaattgga gctgatggcc tagcaatttg gtatgcagaa aatcaaggct
     1801 tggagggccc tgtgtttgga tcagctgatc tgtggaatgg tgttggaata ttttttgatt
     1861 cttttgacaa tgatggaaag aaaaataatc ctgctatagt aattataggc aacaatggac
     1921 aaatccatta tgaccatcaa aatgacgggg ctagtcaagc tttggcaagt tgccagaggg
     1981 acttccgcaa caaaccctat cctgtccgag caaagattac ctattaccag aacacactga
     2041 cagtaatgat caataatggc tttacaccag ataaaaatga ttatgaattt tgtgccaaag
     2101 tggaaaatat gattatccct gcacaagggc attttggaat atctgctgca actggaggtc
     2161 ttgcagatga ccatgatgtc ctttcttttc tgactttcca gttgactgaa cctggaaaag
     2221 agccgcccac accagataaa gaaatttcgg aaaaggaaaa agaaaagtat caggaggaat
     2281 ttgagcactt tcaacaagaa ttggataaaa aaaaagagga attccagaag ggccaccccg
     2341 acctccaagg gcagcctgcg gaggaaatat ttgagagtgt aggagatcga gagctaagac
     2401 aagtctttga aggacagaat cgtattcatc ttgaaatcaa gcagctgaac cggcagttag
     2461 atatgattct tgatgaacag agaagatatg tctcttcctt aacagaggaa atctctaaaa
     2521 gaggagcagg aatgcctggg cagcatgggc agattactca acaagaactg gatactgttg
     2581 tgaaaactca gcatgagatt ctgagacaag taaatgaaat gaaaaattcc ttgagtgaaa
     2641 ccgtcagact ggtcagtgga atgcagcacc ctggctctgc tggaggcgtc tatgagacaa
     2701 cacagcactt cattgacatc aaagagcacc tgcacatagt aaagagggac atagataact
     2761 tagtgcagcg aaatatgcca tcaaatgaaa agccgaaatg cccagaacta ccaccatttc
     2821 catcatgttt gtctacggtc cacttcatta tatttgttgt ggtgcaaact gtattattca
     2881 ttggttatat catgtatagg tctcagcaag aagcagctgc caaaaaattc ttttgaggat
     2941 ccctcgagcg gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     3001 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3061 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3181 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     3241 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3301 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3361 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3421 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3481 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3541 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3601 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3661 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3781 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4081 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4141 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4201 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4261 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4321 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4381 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4441 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4501 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4561 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4621 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4681 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4741 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4801 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4861 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4921 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4981 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5041 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5101 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5161 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5221 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5281 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5341 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5401 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6301 taccgccatg cat
//
