LOCUS       pEA01                  10632 bp    DNA     circular SYN 23-APR-2021
DEFINITION  Ligation of Fragment 1 into pRL6ermE clone E final*
ACCESSION   pEA01
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pRL6ermE clone E final*
               Fragment ends: EcoRV
               Fragment size: 7441
            Insert molecule:  Fragment 1
               Fragment ends: blunt and SspI
               Fragment size: 3030
FEATURES             Location/Qualifiers
     CDS             366..1160
                     /gene="aph(3')"
                     /product="Kan/Neo"
                     /SECDrawAs="Gene"
     misc_feature    1608..2481
                     /gene="pBR-ori"
                     /SECDrawAs="Region"
     misc_feature    2788..4122
                     /gene="pA-rep "
                     /SECDrawAs="Region"
     CDS             5714..6313
                     /gene="pac"
                     /SECDrawAs="Gene"
     misc_feature    6505..6613
                     /gene="oriT"
                     /SECDrawAs="Region"
     CDS             complement (7205..8371)
                     /gene="int"
                     /SECDrawAs="Gene"
     CDS             complement (8371..8559)
                     /gene="xis"
                     /SECDrawAs="Gene"
     promoter        complement (8627..8656)
                     /gene="trc p"
                     /SECDrawAs="Gene"
     misc_signal     8873..9032
                     /gene="seq add"
                     /SECDrawAs="Label"
     CDS             9222..10367
                     /gene="ermE"
                     /SECDrawAs="Gene"
ORIGIN      
        1 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
       61 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
      121 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
      181 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
      241 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
      301 ctgatggcgc aggggatcaa gatccccggg gatctgatca agagacagga tgaggatcgt
      361 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      421 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      481 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      541 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
      601 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
      661 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
      721 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
      781 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
      841 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
      901 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
      961 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     1021 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     1081 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     1141 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     1201 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     1261 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1321 cttcgcccac cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga
     1381 cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg
     1441 ctatccgcgc atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt
     1501 cgatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1561 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2221 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2461 tcaaaaagga tcttcaccta gccacccgat gagccgccgc cgcgccgtcc agcgaggagc
     2521 ccgccgggcc gagcccgtcg acccgcacgc gtccatcgag ggccagctgg ccctgttcgg
     2581 cgacgacccg cagcccgagc ccgtcaaggc cgagctggcc gccgtcgacc cggcggaggc
     2641 gtcccgatga acccgcggaa caacgccgac gacgccagcg acgccgccgt gggctggacc
     2701 gaggcccggt ggctggtctc caacggcaag gcgaagtagt cctgaccgtc gaccaggtgg
     2761 tgcccgccgg gccgttgccc ggcgggcact gtcgcgttcg gatgcacgga gaccagggga
     2821 aacgtggacg tctgacgatg atcggccgta ggatcacgga cgcgcccgac gcgcaaacgg
     2881 tccgcgtcgg ccgcgagagg cgggttaccc ccggcccgcc accctctcgc gtggcattcg
     2941 cccgaacgtg gcaacttttc cggcgtgatc atcggcgtgt cgctcgtttt cgcgtactcg
     3001 aaacgataga acgtgccgta tgggacgacc gaagggcaga cccccgcgct gggacgccga
     3061 cgttcagctg aaggtgatca aggcccggat gccggtcgag ctggccgagg cgttccagaa
     3121 ggaagcgcag aagcgcggca tgaacgtgca ggacttcctg ggccgactgg ccgaggagct
     3181 gaccggcgtg ccctacaacc cgcagggagg tttgccgctg agcgcctaga acgacgaaaa
     3241 gcgccccgcc aggagttacc agctcccgcg agacgcttcc acgccgctaa ctgaacatcc
     3301 acgcccacca aggccactga ctcgtgaagg gtttcgccgg ccgttggtgc gctgtatcta
     3361 cgcttggagc caccatacca acccggccca ggtgtggccc agtactgccc gcgtgcgaca
     3421 cgcggacgga ttctccaggg tccaccgaat cagcaatctt gacgcttaag cgtcaacctg
     3481 tcagcgactt gtgcgtcagt tgcggccggg cccctggcgt gccacggccc accccgttgc
     3541 ggaccgtcca ctgtggcccg tgctgggccg agttcggcgg gtcgcccgac gtcgccgaga
     3601 tcggcccgcc cccgccggag gctctggggt cgcgcgaccc cagagaccag cgggaatgga
     3661 taacgagcct ggccggtgag cagtgggtcc agatgctccg gagcgactgc cgcgcccgtc
     3721 tgctcgacct cgcacaggag atgatgctcc gcgcgaactg ggacacctgg gagtcgtggc
     3781 caggctggga ccggctcatg gaggtgtccc gctggaagcg ctccgcgctg gccggctggc
     3841 tgcgccagct gcgcctgctc ggctggttgt cagtgatcga gtccggcagc acgccgcagt
     3901 tccggccgat ggtcctgtcc cacgtcgagg gcaaccgccg cgccgtctac gccctgcggg
     3961 tgccccgccg tcccggcgag caggccaccg ccccccaacc gccagcccgt cccgagcccg
     4021 agctggagac cgtcccggcc gagccgttca cccgttctga ccagcgcgga cgcgcgtctg
     4081 tggatagaac ctggacccct acgtggttct ttgatctttc ttctagagtt gatctttggg
     4141 tttcttcgcg cgcgagagat tttttccaca gctcccagct cggcgcgcag gttgagccca
     4201 agacggagcc gcttcgcggc cgctttgacg aggaatggct ggcgttcgct cgaacgtcgc
     4261 cgaccagcgg cgctcagatg ctcgccgcgg ccgccgagct gcgccgccag gagccgatcc
     4321 tggcccagtt gaccgcgcgc gccgtccggg ccctgtgtcg cccgtactgg gctgctggct
     4381 gggcaaacga cgatgtgcgg cacgcgctcc gctggcggcc gcgggccacg tcgagcctgc
     4441 ccgcgatgga gcaggcgaag atccacgcgc cgtaccggtg ggcgcagtcg aggctggccg
     4501 cctggcgcac cgacaccggc aaggttctgc ctggtcacag ccacatggag cgcgcccagc
     4561 ggacccgcga gcacgcggtg gtggcccgct tcggcaaggc cgcgctggcc gcgatgcccg
     4621 ccgggtcgga ccggctgcgc ccggttgacg tgcgccggtt cggccgccgc cgggtcgacg
     4681 ccgcggtgga gacgctgcgc cgccgtcggg ccgccgagct ggccgacgag cgcgccgccg
     4741 tccggtcgca ggccgaggcc gccgaccccg agcacgcccg ccgggtcgcc gacgagttcc
     4801 gcgccgagcg cgagcgccgc caggccgctg gccgcgagcg gctgctggag caggcccgcg
     4861 ccgcggccgc cgagcgggcc gagcaggacc agcccgcccc ggcgagcacg tggacaccgc
     4921 agggaccagc gcaaacgccg gaggagcggc aggaggccgc gctggcccga gcacgggcgg
     4981 aaggccgcgt gcccctggtc cgtcgtcgcc gccggacccg ttggtgatgc ttgcggcgct
     5041 ggcctgggtg gtaccagggg gctcgcgccc cctggacccc ccggcccagc ggcacgaccc
     5101 gcgccggacg ttccagcggt gccggttccg ttggcgcggg ccgcaccgct ggagtggtgc
     5161 cgagggtccg gtgagaaggt ggtcaccagc ccgtcgacca gcaggagcgc gtccgcatga
     5221 ccagccccga tgacctggac gactacctgc gggaactact gcgcagggag aaggccggcg
     5281 agcggcaccg gatcgaggtt ggcccgtaca cggcgatgct ggtcacgggg gcgatccagt
     5341 gggcgatccg gcgcggtgag ctggccgagc acgggccggt gatgttcgag cggctgctgg
     5401 accagctccg gctgctgttc gccgacgacc cgaggggcgc ggagctgatc tatatctatg
     5461 atctcgcagt ctccggcgag caccggaggc agggcattgc caccgcgctc atcaatctcc
     5521 tcaagcatga ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca gattacggtg
     5581 acgatcccgc agtggctctc tatacaaagt tgggcatacg ggaagaagtg atgcactttg
     5641 atggccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac ccctcacaag
     5701 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5761 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5821 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5881 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5941 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6001 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6061 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6121 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6181 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6241 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6301 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     6361 ccatgacgga tcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     6421 gtatatccat cctttctcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     6481 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     6541 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     6601 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
     6661 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
     6721 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
     6781 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
     6841 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac
     6901 ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag
     6961 aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga
     7021 cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg
     7081 ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa
     7141 gaccgatgat agcttgcatg cctgcaggtc gactctagat ttctgtgcgt gtctatctgc
     7201 gtgactatcg cgccggaccc cgcttgaaga tgccgtccat gaccacagcg ccggtctgga
     7261 tgacgggccg gatctgcttc cggtagacct cctcagtcac ggccgtaccg gagtgtccga
     7321 cgagccggga gatctcctcc agcgggacgc cgcggtcgga cagcagggac acgaagctgt
     7381 gcctcagctc cctcggtgtc cactcgtcgg cgttgatccc gttggcatcc ttgagcgcct
     7441 ggcggaaggc gcgccggacg ttagtcgcgt cgagcggctt gccaacggcc gacgagaaga
     7501 ccaggccgtg ttcctcccac ttgtcaccgg cggcgagccg ttcccagccc tggtcctcaa
     7561 agtgctgcca gaggacctcc acgcaacgcg ccggcagggc gagcgttcgc cgagacttcc
     7621 gggttttcgt gtccccaccg cgccggaccg agcgccagac ggcgatgtgc ggaggctgcg
     7681 gcggctcaac gtccggactt cccttgagga agacgtggtc ccaggtcagc gcccgcagct
     7741 cctcggtgcg cgcaccggtc agcagggcga cgacgatgta ggcgtgcatc gacgtgccct
     7801 cggcagcatt cagcaccgcc tcggcctggg cgaaggtgag cgccttggac ggccggccag
     7861 gctggccctg gggcacagag cacagctcca ccacgttgcg cttcaccttg tcacgcgcca
     7921 tggcccgctt gaccgcccgg ttcaggcagg agtggaccgc ctgaaggctg cgcgtgctca
     7981 gagtctgagc cttggcggcc agccagcggt cgacgtcctc tgcgctgagg tcacgcagct
     8041 tccgggcacc caaacccggt atgacgtgct tctggcttag gtgggtgcag ttctcgacgg
     8101 tgcgctggtc acggccagcg agaccgtagg caagccagtc gttcaccgcg tcggcgacgg
     8161 tgtaccccgt gggtgcgatc gcgagaccgt cttcgtggtc acgcagaacc tctttgagct
     8221 tgttcttggc ctccgtcttg gtcttgccac tcccccgctt gacgatccgc ttaccgctcg
     8281 gatcgaagcc gaggttcgcc gtggcgatcc agcgctgtct cttctcgtcc cagtggaggc
     8341 cgccgtcacc ccggctacgt cgcttggcca tcagatggcc tctcccaact ggtcctgaat
     8401 gaagtcgcgt acggcgtcgg tgggtactcg acgcgctccg tcgatcttga tggaggcaag
     8461 gcggcgggtg cggatgaggt cgtagacctt gctgcgtcca accttcagcc gcgccatgac
     8521 ttccggcacg gtcagcagct ctggagtgac ggtggtcatg gtaccttcct cccgaattcc
     8581 atggtctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg
     8641 gatgattaat tgtcaacagc tcatttcaga atatcgtcca ttccgacagc atcgccagtc
     8701 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg
     8761 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca
     8821 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatctagaat tcgagctcgg
     8881 tacccgggga tctgcagcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     8941 taccagcccg acccgagcac gcgccggcac gcctggtcga tgtcggaccg gagttcgagg
     9001 tacgcggctt gcaggtccag gaaggggacg tccatgcgag tgtccgttcg agtggcggct
     9061 tgcgcccgat gctagtcgcg gttgatcggc gatcgcaggt gcacgcggtc gatcttgacg
     9121 gctggcgaga ggtgcgggga ggatctgacc gacgcggtcc acacgtggca ccgcgatgct
     9181 gttgtgggct ggacaatcgt gccggttggt aggatccagc ggtgagcagt tcggacgagc
     9241 agccgcgccc gcgtcgccgc aaccaggatc ggcagcaccc caaccagaac cggccggtgc
     9301 tgggccgtac cgagcgggac cgcaaccggc gccagttcgg gcagaacttc ctccgcgacc
     9361 gcaagaccat cgcgcgcatc gccgagacag ccgagctgcg gcccgatctg ccggtgctgg
     9421 aagccggccc cggcgaaggg ctgctcacca gggaactcgc cgaccgcgcg cgtcaggtga
     9481 cgtcgtacga gatcgacccc cggctggcga agtcgttgcg ggagaagctt tccggccacc
     9541 cgaacatcga agtcgtcaac gccgacttcc tcaccgccga accgccgccc gagccgttcg
     9601 ccttcgtcgg cgcgatcccc tacggcatca cctcggcgat cgtggactgg tgcctggagg
     9661 cgccgacgat cgagacggcg acgatggtca cgcagctgga gttcgcccgg aagcggaccg
     9721 gcgattacgg ccgctggagc cgcctcacgg tgatgacctg gccgctgttc gagtgggagt
     9781 tcgtcgagaa ggtcgaccgc cggctgttca agccggtgcc caaggtcgac tcggcgatca
     9841 tgcggctgcg caggcgcgcc gaaccgctgc tggaaggcgc ggcgctcgaa cgctacgagt
     9901 cgatggtcga gctgtgcttc accggcgtcg gcggcaacat ccaggcgtcg cttctgcgca
     9961 agtacccgag gcgccgcgtc gaggcggcgc tcgaccacgc gggggtcggg ggcggcgccg
    10021 tggtcgccta cgtccggccg gagcagtggc tccggctgtt cgagcggctg gatcagaaga
    10081 acgaaccgag gggtgggcag ccccagcggg gcaggcgaac cggcggacgg gaccacgggg
    10141 accggcgaac cggcgggcag gatcgcggcg atcggcgaac cggcggccgc gaccacaggg
    10201 accggcaagc cagcggccac ggcgatcgtc gcagcagcgg acgcaatcgc gacgacggac
    10261 gaaccggcga gcgcgagcag ggggaccaag gcgggcggcg ggggccgtcc gggggtggac
    10321 ggaccggcgg acgtccaggg cgacgcggcg gacccgggca gcggtagtcc ccggcacgcg
    10381 gaacggggca ggccgtcgag cggcctgccc cgttctgtcg agaggaatca gaggttgatg
    10441 tcggcccgga ggtcgatgtc gcgcgacgac gagccgatct ccaccgctcg cttgccgccc
    10501 ccgagcttcc agccgcccgc ggcttcgtcc cagtgctgga gggcccgctc cgcgacgtgc
    10561 acccggacgc gcttggtctc gcccggtgcg agttcgacct tctggtaccg agctcgaatt
    10621 cagatccttt aa
//
