LOCUS       OR_GATE_ALP_and_csgAHis 4597 bp ds-DNA     circular     27-SEP-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      98..192
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(204..863)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1198..1252
                     /label="TP901 attB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1253..1396)
                     /label="proD"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1397..1462)
                     /label="TP901 attP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1463..1468
                     /label="EcoRI"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1469..1483
                     /label="RBS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1469..1514
                     /label="pOA3"
                     /note="sequence: gcaattaagcgagttggaGAATTCATTAAAGAGGAGAAAGGTACCATGAAACAAAGCACTATTGCACTGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1489..1489
                     /label="5'GibSeq (concatenated sequence 1)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1489..1489
                     /label="Extracted region from null"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1490..1552
                     /label="ALP N21"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1490..2905
                     /label="ALP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1490..2905
                     /label="Translation 1490-2905"
     misc_feature    2913..2916
                     /label="Ligation"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2918..3022
                     /label="rrnB T1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3005..3022)
                     /label="pOA4"
                     /note="sequence: gtggttgaccagacaaaccacgacACGCGTTCTAGGGCGGCGGATTTG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3023..3028
                     /label="MluI"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    3029..3086
                     /label="Bxb1 attP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(3092..3235)
                     /label="proD"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(3241..3290)
                     /label="Bxb1 attP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             3302..3314
                     /label="BBa_B0032"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            3321..3791
                     /label="csgA His"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             3321..3791
                     /label="Translation 3321-3791"
     CDS             3774..3791
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3795..3874
                     /label="BBa_B0010"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4036..4581
                     /label="p15A ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 ATCAGATAAA ATATTTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG
       61 TCAGCCCCAT ACGATATAAG TTGTTACTAG TGCTTGGATT CTCACCAATA AAAAACGCCC
      121 GGCGGCAACC GAGCGTTCTG AACAAATCCA GATGGAGTTC TGAGGTCATT ACTGGATCTA
      181 TCAACAGGAG TCCAAGCGAG CTCccaatgc ttaatcagtg aggcacctat ctcagcgatc
      241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      481 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
      541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
      841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgACGTCctg
     1201 ataattgcca acacaattaa catctcaatc aaggtaaatg ctttttcgtt ttAAAGTTAA
     1261 ACAAAATTAT TTGTAGAGGG AAACCGTTGT GGTCTCCCTG AATATATTAT ACGAGCCTTA
     1321 TGCATGCCCG TAAAGTTATC CAGCAACCAC TCATAGACCT AGGGCAGCAG ATAGGGACGA
     1381 CGTGGTGTTA GCTGTGaaag gagtttttta gttaccttaa ttgaaataaa cgaaataaaa
     1441 actcgcaatt aagcgagttg gaGAATTCAT TAAAGAGGAG AAAGGTACCA TGAAACAAAG
     1501 CACTATTGCA CTGGCACTCT TACCGTTACT GTTTACCCCT GTGACAAAAG CCCGGACACC
     1561 AGAAATGCCT GTTCTGGAAA ACCGGGCTGC TCAGGGCGAT ATTACTGCAC CCGGCGGTGC
     1621 TCGCCGTTTA ACGGGTGATC AGACTGCCGC TCTGCGTGAT TCTCTTAGCG ATAAACCTGC
     1681 AAAAAATATT ATTTTGCTGA TTGGCGATGG GATGGGGGAC TCGGAAATTA CTGCCGCACG
     1741 TAATTATGCC GAAGGTGCGG GCGGCTTTTT TAAAGGTATA GATGCCTTAC CGCTTACCGG
     1801 GCAATACACT CACTATGCGC TGAATAAAAA AACCGGCAAA CCGGACTACG TCACCGACTC
     1861 GGCTGCATCA GCAACCGCCT GGTCAACCGG TGTCAAAACC TATAACGGCG CGCTGGGCGT
     1921 CGATATTCAC GAAAAAGATC ACCCAACGAT TCTGGAAATG GCAAAAGCCG CAGGTCTGGC
     1981 GACCGGTAAC GTTTCTACCG CAGAGTTGCA GGATGCCACG CCCGCTGCGC TGGTGGCACA
     2041 TGTGACCTCG CGCAAATGCT ACGGTCCGAG CGCGACCAGT GAAAAATGTC CGGGTAACGC
     2101 TCTGGAAAAA GGCGGAAAAG GATCGATTAC CGAACAGCTG CTTAACGCTC GTGCCGACGT
     2161 TACGCTTGGC GGCGGCGCAA AAACCTTTGC TGAAACGGCA ACCGCTGGTG AATGGCAGGG
     2221 AAAAACGCTG CGTGAACAGG CACAGGCGCG TGGTTATCAG TTGGTGAGCG ATGCTGCCTC
     2281 ACTGAATTCG GTGACGGAAG CGAATCAGCA AAAACCCCTG CTTGGCCTGT TTGCTGACGG
     2341 CAATATGCCA GTGCGCTGGC TAGGACCGAA AGCAACGTAC CATGGCAATA TCGATAAGCC
     2401 CGCAGTCACC TGTACGCCAA ATCCGCAACG TAATGACAGT GTACCAACCC TGGCGCAGAT
     2461 GACCGACAAA GCCATTGAAT TGTTGAGTAA AAATGAGAAA GGCTTTTTCC TGCAAGTTGA
     2521 AGGTGCGTCA ATCGATAAAC AGGATCATGC TGCGAATCCT TGTGGGCAAA TTGGCGAGAC
     2581 GGTCGATCTC GATGAAGCCG TACAACGGGC GCTGGAATTC GCTAAAAAGG AGGGTAACAC
     2641 GCTGGTCATA GTCACCGCTG ATCACGCCCA CGCCAGCCAG ATTGTTGCGC CGGATACCAA
     2701 AGCTCCGGGC CTCACCCAGG CGCTAAATAC CAAAGATGGC GCAGTGATGG TGATGAGTTA
     2761 CGGGAACTCC GAAGAGGATT CACAAGAACA TACCGGCAGT CAGTTGCGTA TTGCGGCGTA
     2821 TGGCCCGCAT GCCGCCAATG TTGTTGGACT GACCGACCAG ACCGATCTCT TCTACACCAT
     2881 GAAAGCCGCT CTGGGGCTGA AATAActcga gACGCGTGGC ATCAAATAAA ACGAAAGGCT
     2941 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
     3001 AGGACAAATC CGCCGCCCTA GAACGCGTgt cgtggtttgt ctggtcaacc accgcggtct
     3061 cagtggtgta cggtacaaac cccgacGGTA CAAAGTTAAA CAAAATTATT TGTAGAGGGA
     3121 AACCGTTGTG GTCTCCCTGA ATATATTATA CGAGCCTTAT GCATGCCCGT AAAGTTATCC
     3181 AGCAACCACT CATAGACCTA GGGCAGCAGA TAGGGACGAC GTGGTGTTAG CTGTGTTAAG
     3241 gcccggatga tcctgacgac ggagaccgcc gtcgtcgaca agccggccga CCCGGCTTAA
     3301 Gtcacacagg aaagGGATCC ATGAAACTTT TAAAAGTAGC AGCAATTGCA GCAATCGTAT
     3361 TCTCCGGTAG CGCTCTGGCA GGTGTTGTTC CTCAGTACGG CGGCGGCGGT AACCACGGTG
     3421 GTGGCGGTAA TAATAGCGGC CCAAATTCTG AGCTGAACAT TTACCAGTAC GGTGGCGGTA
     3481 ACTCTGCACT TGCTCTGCAA ACTGATGCCC GTAACTCTGA CTTGACTATT ACCCAGCATG
     3541 GCGGCGGTAA TGGTGCAGAT GTTGGTCAGG GCTCAGATGA CAGCTCAATC GATCTGACCC
     3601 AACGTGGCTT CGGTAACAGC GCTACTCTTG ATCAGTGGAA CGGCAAAAAT TCTGAAATGA
     3661 CGGTTAAACA GTTCGGTGGT GGCAACGGTG CTGCAGTTGA CCAGACTGCA TCTAACTCCT
     3721 CCGTCAACGT GACTCAGGTT GGCTTTGGTA ACAACGCGAC CGCTCATCAG TACCACCACC
     3781 ACCACCACCA CTAAccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3841 tcgttttatc tgttgtttgt cggtgaacgc tctcGGTACC CCGGGGGATA TATTCCGCTT
     3901 CCTCGCTCAC TGACTCGCTA CGCTCGGTCG TTCGACTGCG GCGAGCGGAA ATGGCTTACG
     3961 AACGGGGCGG AGATTTCCTG GAAGATGCCA GGAAGATACT TAACAGGGAA GTGAGAGGGC
     4021 CGCGGCAAAG CCGTTTTTCC ATAGGCTCCG CCCCCCTGAC AAGCATCACG AAATCTGACG
     4081 CTCAAATCAG TGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     4141 CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC CTTTCGGTTT ACCGGTGTCA TTCCGCTGTT
     4201 ATGGCCGCGT TTGTCTCATT CCACGCCTGA CACTCAGTTC CGGGTAGGCA GTTCGCTCCA
     4261 AGCTGGACTG TATGCACGAA CCCCCCGTTC AGTCCGACCG CTGCGCCTTA TCCGGTAACT
     4321 ATCGTCTTGA GTCCAACCCG GAAAGACATG CAAAAGCACC ACTGGCAGCA GCCACTGGTA
     4381 ATTGATTTAG AGGAGTTAGT CTTGAAGTCA TGCGCCGGTT AAGGCTAAAC TGAAAGGACA
     4441 AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG TTACCTCGGT TCAAAGAGTT GGTAGCTCAG
     4501 AGAACCTTCG AAAAACCGCC CTGCAAGGCG GTTTTTTCGT TTTCAGAGCA AGAGATTACG
     4561 CGCAGACCAA AACGATCTCA AGAAGATCAT CTTATTA
//