LOCUS       Exported                4638 bp ds-DNA     circular SYN 20-AUG-2021
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 20, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          921..1310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          929..934
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          929..934
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1300..1305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          1311..1495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          join(1496..4638,1..243)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     primer_bind     57..76
                     /label=3750
                     /note="color: black; sequence: CGGACAGGTATCCGGTAAGC"
     primer_bind     complement(75..87)
                     /label=4852
                     /note="color: black; sequence: 
                     GGATCCggtaccctcccccgatcctgccgc"
     primer_bind     complement(75..87)
                     /label=4854
                     /note="color: black; sequence: 
                     atccattggccggccctcccccgatcctgccgc"
     primer_bind     complement(75..87)
                     /label=3915
                     /note="color: black; sequence: 
                     CGGCCGCctatggccggccctcccccgatcctgccgc"
     primer_bind     complement(75..87)
                     /label=3913
                     /note="color: black; sequence: 
                     CTTGTCcattgggccactagggccctcccccgatcctgccgc"
     primer_bind     complement(76..87)
                     /label=3909
                     /note="color: black; sequence: 
                     atccattggccggccctcccccgatcctgccg"
     primer_bind     79..97
                     /label=1215
                     /note="color: black; sequence: CAGGGTCGGAACAGGAGAG"
     primer_bind     complement(143..160)
                     /label=7564
                     /note="color: black; sequence: GTGGCGAAACCCGACAGG"
     primer_bind     148..167
                     /label=6878
                     /note="color: black; sequence: Cgggtttcgccacctctgac"
     primer_bind     150..173
                     /label=6981
                     /note="color: black; sequence: ggtttcgccacctctgacttgagc"
     primer_bind     complement(150..173)
                     /label=6980
                     /note="color: black; sequence: gctcaagtcagaggtggcgaaacc"
     primer_bind     174..198
                     /label=3469
                     /note="color: black; sequence: GTCGATTTTTGTGATGCTCGTCAGG"
     primer_bind     175..198
                     /label=3175
                     /note="color: black; sequence: 
                     CGATCTCCCGATCCGTCGATCTCGATTTTTGTGATGCTCGTCAGG"
     primer_bind     176..198
                     /label=3095
                     /note="color: black; sequence: CGATTTTTGTGATGCTCGTCAGG"
     primer_bind     199..230
                     /label=448
                     /note="color: black; sequence: 
                     AGNNNNNNAGCGAACCGAGCCAGAAACG"
     primer_bind     complement(201..220)
                     /label=5113
                     /note="color: black; sequence: 
                     gacaaaggactaaatttagtactgtttttccataggctccgcc"
     primer_bind     complement(210..235)
                     /label=290
                     /note="color: black; sequence: 
                     ACGCGTGGTACCGGCCGCGTTGCTGGCGTTTTTCCATA"
     primer_bind     complement(214..235)
                     /label=2277
                     /note="color: black; sequence: 
                     tagcacgcgtcggccgcgttgctggcgtttttc"
     primer_bind     complement(214..235)
                     /label=4619
                     /note="color: black; sequence: 
                     ctagtccatggacgagtggtaacggccgcgttgctggcgtttttc"
     primer_bind     complement(220..235)
                     /label=7285
                     /note="color: black; sequence: 
                     gtgaatcgaatcctaagagaattgacgcgtggtaccggccgcgttgctggcg"
     primer_bind     complement(225..234)
                     /label=4070
                     /note="color: black; sequence: 
                     GCTTTTTTGTACAGCTAGCGACGTCaccggggcgccgcggtgc"
     primer_bind     242..262
                     /label=2744
                     /note="color: black; sequence: cggttcctggccttttgctgg"
     primer_bind     complement(251..278)
                     /label=6155
                     /note="color: black; sequence: 
                     TGACATGTGAGCAAAAGGCCAGCAAAAGGC"
     primer_bind     complement(251..270)
                     /label=6126
                     /note="color: black; sequence: 
                     ggcaaagtttagagcactgcccctccgcacgcaaaaggccagcaaaaggc"
     primer_bind     complement(252..273)
                     /label=3417
                     /note="color: black; sequence: 
                     accaggtcctgcaggcttaagggtaccTGAGCAAAAGGCCAGCAAAAGG"
     primer_bind     complement(254..274)
                     /label=1819
                     /note="color: black; sequence: 
                     GCTCGAAGCCGCGGTGAGCAAAAGGCCAGCAAAA"
     primer_bind     complement(258..278)
                     /label=4472
                     /note="color: black; sequence: 
                     GCAACCACAAACCCACGAGCATATGACATGTGAGCAAAAGGCCAGC"
     primer_bind     complement(258..278)
                     /label=4470
                     /note="color: black; sequence: 
                     GGTTGACAAGTTTGGCTGGGCATATGACATGTGAGCAAAAGGCCAGC"
     primer_bind     complement(258..278)
                     /label=4476
                     /note="color: black; sequence: 
                     GCATTAAGCGCGGCGGGTGTGGCATATGACATGTGAGCAAAAGGCCAGC"
     primer_bind     complement(258..278)
                     /label=4474
                     /note="color: black; sequence: 
                     CCTCTCTACATACATTGATTATTGACTAGCATATGACATGTGAGCAAAAGGCCAGC"
     primer_bind     complement(262..271)
                     /label=6155
                     /note="color: black; sequence: 
                     TGACATGTGAGCAAAAGGCCAGCAAAAGGC"
     primer_bind     complement(262..271)
                     /label=6126
                     /note="color: black; sequence: 
                     ggcaaagtttagagcactgcccctccgcacgcaaaaggccagcaaaaggc"
     primer_bind     complement(263..295)
                     /label=3314
                     /note="color: black; sequence: 
                     GGATAACGCAGGAAAGAACATGACATGTGAGCAAAAGG"
     primer_bind     263..295
                     /label=3313
                     /note="color: black; sequence: 
                     CCTTTTGCTCACATGTCATGTTCTTTCCTGCGTTATCC"
     primer_bind     288..305
                     /label=6755
                     /note="color: black; sequence: CGTTATCCCCTGATTCTG"
     primer_bind     complement(296..316)
                     /label=5727
                     /note="color: black; sequence: 
                     Ggcggccgccacggcgatatcggatctcgagacggttatccacagaatcagg"
     primer_bind     complement(297..317)
                     /label=5982
                     /note="color: black; sequence: 
                     AGGAAAGAATATTTAGAGAAAAGAAGAAAACAAGAGTACTacggttatccacagaatca
                     g"
     primer_bind     complement(299..317)
                     /label=2103
                     /note="color: black; sequence: 
                     catatgagaccgactacggttatccacagaatc"
     primer_bind     complement(299..317)
                     /label=2218
                     /note="color: black; sequence: 
                     gaattcCCTGTTTCGcgtctctcatgaGGATtgagaccgactacggttatccacagaat
                     c"
     primer_bind     complement(325..343)
                     /label=5000
                     /note="color: black; sequence: 
                     catcctcttcgttGGTATCAGCTCACTCAAAG"
     primer_bind     complement(341..355)
                     /label=5674
                     /note="color: black; sequence: TTTATGCTGTGGCGATCGGT"
     primer_bind     complement(347..363)
                     /label=5757
                     /note="color: black; sequence: 
                     agacgtactaatcttccaataatctcacttttagtactgtcgttcggctgcggcg"
     primer_bind     357..372
                     /label=3050
                     /note="color: black; sequence: 
                     CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAG"
     primer_bind     complement(367..376)
                     /label=6702
                     /note="color: black; sequence: aaggatcaggacgctcgctgcgc"
     primer_bind     complement(401..419)
                     /label=4989
                     /note="color: black; sequence: 
                     ctgtctgagtccgCGGTTTGCGTATTGGGCG"
     primer_bind     432..453
                     /label=4999
                     /note="color: black; sequence: 
                     ccggatccagttgGCCGATTCATTAATGCAG"
     primer_bind     complement(473..492)
                     /label=1060
                     /note="color: black; sequence: 
                     gagctcgaatTCACTGCCCGCTTTCCAGTC"
     primer_bind     complement(487..526)
                     /label=7545
                     /note="color: black; sequence: 
                     gtaattcatatattatagtactattgcgttgcgctcactg"
     primer_bind     487..526
                     /label=7544
                     /note="color: black; sequence: 
                     cagtgagcgcaacgcaatagtactataatatatgaattac"
     primer_bind     complement(487..504)
                     /label=7402
                     /note="color: black; sequence: 
                     atttaattaaattatgtactgaaaaaatagatagtactATTAATTGCGTTGCGCTCACT
                     G"
     primer_bind     505..564
                     /label=6535
                     /note="color: black; sequence: 
                     agtactataatatatgaattacattattaatttaaaaacgcgtgatgacctatacatag
                     g"
     primer_bind     506..570
                     /label=5965
                     /note="color: black; sequence: 
                     gtactataatatatgaattacattattaatttaaaagatgacctatacataggaagatc
                     "
     misc_feature    508..541
                     /label=flank 1
                     /note="color: #a6acb3"
     primer_bind     complement(508..541)
                     /label=5472
                     /note="color: black; sequence: 
                     ggctgtttgtggctctaccatctagattttaaattaataatgtaattcatatattatag
                     t"
     CDS             508..510
                     /codon_start=1
                     /label=pGKL1 ORF3 delta ORF2 delta
                     /note="color: #993366"
                     /translation="T"
     primer_bind     527..567
                     /label=3803
                     /note="color: black; sequence: 
                     attattaatttaaaaGATGACCTATACATAGGAAG"
     CDS             541
                     /codon_start=1
                     /label=pGKL1 ORF3 delta ORF2 delta
                     /note="color: #993366"
                     /translation=""
     primer_bind     542..567
                     /label=138
                     /note="color: black; sequence: 
                     CATGACGCGTGATGACCTATACATAGGAAG"
     misc_feature    complement(542..547)
                     /label=MluI
                     /note="color: #0080ff; direction: LEFT"
     primer_bind     543..594
                     /label=6282
                     /note="color: black; sequence: 
                     aaacgccgcgggatgacctatacataggaagatctatagaaacaaaaagattaataac"
     primer_bind     543..568
                     /label=4591
                     /note="color: black; sequence: 
                     atagaaacgccgcggGATGACCTATACATAGGAAGA"
     primer_bind     543..567
                     /label=722
                     /note="color: black; sequence: 
                     aattattataaaaactttcatataccgcggGATGACCTATACATAGGAAG"
     primer_bind     543..563
                     /label=4674
                     /note="color: black; sequence: 
                     aatagaaacgCCGCGGGATGACCTATACATAG"
     primer_bind     543..563
                     /label=873
                     /note="color: black; sequence: 
                     gaaattcataatagaaacgCCGCGGGATGACCTATACATAG"
     primer_bind     544..576
                     /label=4300
                     /note="color: black; sequence: 
                     cccgagctcAAGCTTGATGACCTATACATAGGAAGATCTATAGA"
     primer_bind     complement(546..567)
                     /label=1976
                     /note="color: black; sequence: CTTCCTATGTATAGGTCATCAC"
     5'UTR           548..647
                     /label=p10B2
                     /label=UTR from 2ORF10
                     /note="color: #a6acb3"
     primer_bind     complement(548..607)
                     /label=5531
                     /note="color: black; sequence: 
                     ctgatatttgaaagttattaatctttttgtttctgtagatcttcctatgtataggtcat
                     c"
     primer_bind     complement(548..594)
                     /label=5854
                     /note="color: black; sequence: 
                     gttattaatctttttgtttctgtagatcttcctatgtataggtcatc"
     primer_bind     548..594
                     /label=5853
                     /note="color: black; sequence: 
                     gatgacctatacataggaagatctacagaaacaaaaagattaataac"
     primer_bind     complement(548..594)
                     /label=5855
                     /note="color: black; sequence: 
                     gaggttattaatctttttgtttctgtagatcttcctatgtataggtcatc"
     primer_bind     548..579
                     /label=4182
                     /note="color: black; sequence: 
                     gtctggtctcacagcgatgacctatacataggaagatctatagaaac"
     primer_bind     548..570
                     /label=573
                     /note="color: black; sequence: GATGACCTATACATAGGAAGATC"
     primer_bind     548..570
                     /label=582
                     /note="color: black; sequence: 
                     agtctgaggggatccGATGACCTATACATAGGAAGATC"
     primer_bind     548..567
                     /label=3996
                     /note="color: black; sequence: 
                     tttattaatattaGATGACCTATACATAGGAAG"
     primer_bind     548..567
                     /label=2176
                     /note="color: black; sequence: 
                     gatcGGTCTCAAACGGATGACCTATACATAGGAAG"
     primer_bind     complement(551..602)
                     /label=5852
                     /note="color: black; sequence: 
                     atttggaagttattaatctttttgtttctgtagatcttcctatgtataggtc"
     primer_bind     complement(551..585)
                     /label=3917
                     /note="color: black; sequence: 
                     ctttttgtttctatagatGttcctatgtataggtc"
     primer_bind     551..585
                     /label=3916
                     /note="color: black; sequence: 
                     gacctatacataggaaCatctatagaaacaaaaag"
     primer_bind     555..585
                     /label=6798
                     /note="color: black; sequence: 
                     tatacataggaagatctatagaaacaaaaag"
     primer_bind     complement(570..620)
                     /label=5529
                     /note="color: black; sequence: 
                     tatttctacatttttctgatatttggaagttattaatctttttgtttctatag"
     primer_bind     complement(570..620)
                     /label=5530
                     /note="color: black; sequence: 
                     atatttctacatttttctgatatttgaaggttattaatctttttgtttctatag"
     primer_bind     574..617
                     /label=5851
                     /note="color: black; sequence: 
                     ctacagaaacaaaaagattaataacttccaaatatcagaaaaatgtag"
     primer_bind     574..607
                     /label=5528
                     /note="color: black; sequence: 
                     agaaacaaaaagattaataactttcaaatatcag"
     primer_bind     complement(575..623)
                     /label=5850
                     /note="color: black; sequence: 
                     tatgtttctacatttttctgatatttggaagttattaatctttttgtttc"
     primer_bind     575..617
                     /label=5843
                     /note="color: black; sequence: 
                     gaaacaaaaagattaataacctccaaatatcagaaaaatgtag"
     primer_bind     complement(575..613)
                     /label=5844
                     /note="color: black; sequence: 
                     ctacatttttctgatatttggaggttattaatctttttgtttc"
     primer_bind     576..634
                     /label=6267
                     /note="color: black; sequence: 
                     aaacaaaaagattaataactttcaaatatcagaaaAaatgtagaaatatatgataagct
                     c"
     primer_bind     complement(579..638)
                     /label=7754
                     /note="color: black; sequence: 
                     ctatgagcttatcaCATGTTTCTACATTTTTCTGATATTTGAAAGTTATTAATCTTTTT
                     G"
     primer_bind     579..634
                     /label=5523
                     /note="color: black; sequence: 
                     caaaaagattaataactttcaaatatcagaaaaatgtagaaacatatgataagctc"
     primer_bind     complement(579..608)
                     /label=5526
                     /note="color: black; sequence: 
                     tctgatatttgaaagttattaatctttttg"
     primer_bind     582..640
                     /label=5524
                     /note="color: black; sequence: 
                     aaagattaataactttcaaatatcagaaaaatgtagaaatatgtgataagctcatagac
                     "
     primer_bind     complement(584..613)
                     /label=4670
                     /note="color: black; sequence: 
                     gcttatcatatattcCTACATTTTTCTGATATTTGAAAGTTATTAATCT"
     primer_bind     complement(594..642)
                     /label=6278
                     /note="color: black; sequence: 
                     cattttacatgtctatgagcttatcacatgtttctacatttttctgatatttgaaag"
     primer_bind     complement(594..620)
                     /label=6268
                     /note="color: black; sequence: 
                     atcatatatttctacatttttctgatatttgaaag"
     primer_bind     complement(594..610)
                     /label=6627
                     /note="color: black; sequence: TTTCTGATATTTGAAAG"
     primer_bind     complement(598..656)
                     /label=6671
                     /note="color: black; sequence: 
                     GCATggatcctttacatgtctatgagcttatcacatgtttctacatttttctgatattt
                     g"
     primer_bind     complement(598..638)
                     /label=5848
                     /note="color: black; sequence: 
                     ctatgagcttatcacatgttcctacatttttctgatatttg"
     primer_bind     complement(598..638)
                     /label=5846
                     /note="color: black; sequence: 
                     ctatgagcttatcacatgtttctacatttttctgatatttg"
     primer_bind     complement(605..639)
                     /label=6763
                     /note="color: black; sequence: 
                     tctatgagcttatcacatgtttctacatttttctg"
     primer_bind     complement(607..642)
                     /label=3959
                     /note="color: black; sequence: 
                     caacatgcatTTTACATGTCTATGAGCTTATCATATATTTCTACATTTTTC"
     primer_bind     608..640
                     /label=6762
                     /note="color: black; sequence: 
                     aaaaatgtagaaacatgtgataagctcatagac"
     primer_bind     609..638
                     /label=4429
                     /note="color: black; sequence: 
                     actataatatatgaattacattattaatttaaaatgtagaaatatatgataagctcata
                     g"
     primer_bind     610..647
                     /label=7751
                     /note="color: black; sequence: 
                     aaatgtagaaacatgTGATAAGCTCATAGACATGTAAA"
     primer_bind     611..648
                     /label=6536
                     /note="color: black; sequence: 
                     aatgtagaaacatgtgataagctcatagacatgtaaag"
     primer_bind     611..640
                     /label=1906
                     /note="color: black; sequence: 
                     tgaataatctacagaatggaaaatgtgtataatgtagaaatatatgataagctcataga
                     c"
     misc_feature    614
                     
                     /note="color: #ff0000"
     primer_bind     complement(621..642)
                     /label=7400
                     /note="color: black; sequence: tttacatgtctatgagcttatcaCatG"
     primer_bind     625..647
                     /label=790
                     /note="color: black; sequence: 
                     attaacgcgttgtagaaatatatgataagctcatagacatgtaaa"
     primer_bind     626..658
                     /label=6810
                     /note="color: black; sequence: 
                     GATAAGCTCATAGACATGTAAAGATATATGGTCTCAGATCTATGAG"
     primer_bind     complement(626..656)
                     /label=2177
                     /note="color: black; sequence: 
                     gatcGGTCTCACATAGATCTTTTACATGTCTATGAGCTTATC"
     primer_bind     complement(626..648)
                     /label=6811
                     /note="color: black; sequence: 
                     CTCATagatctgagaccatatatctttacatgtctatgagcttatc"
     primer_bind     complement(626..642)
                     /label=139
                     /note="color: black; sequence: 
                     CATGATGCATTTTACATGTCTATGAGCTTATC"
     primer_bind     complement(626..642)
                     /label=3995
                     /note="color: black; sequence: 
                     cgctcacatgcatTTTACATGTCTATGAGCTTATC"
     primer_bind     complement(626..642)
                     /label=1166
                     /note="color: black; sequence: 
                     GAAGTAACTGCATTTTACATGTCTATGAGCTTATC"
     primer_bind     complement(626..642)
                     /label=3804
                     /note="color: black; sequence: 
                     gacttgttccatcatTTTACATGTCTATGAGCTTATC"
     primer_bind     complement(626..642)
                     /label=3842
                     /note="color: black; sequence: 
                     ttcgtAGATCTatgagatttacatgtctatgagcttatc"
     primer_bind     complement(631..658)
                     /label=7503
                     /note="color: black; sequence: 
                     ctccttaatcagctcgctcacggatcctttacatgtctatgagc"
     primer_bind     complement(631..656)
                     /label=1751
                     /note="color: black; sequence: 
                     GAAAAACAGCGAAGTAACTGCATggatcctttacatgtctatgagc"
     primer_bind     complement(632..654)
                     /label=1646
                     /note="color: black; sequence: 
                     actgTCATGAattacagtggatcctttacatgtctatgag"
     misc_feature    643
                     /label=-4 GA mutation
                     /note="color: #ff0000"
     primer_bind     649..671
                     /label=6660
                     /note="color: black; sequence: 
                     atatatggtctcagatctATGAGATTCCCATCTATC"
     misc_feature    654..911
                     /label=app8 leader
                     /note="color: #cc99ff"
     primer_bind     700..712
                     /label=4858
                     /note="color: black; sequence: 
                     atccattggccggcccccggatccTGCCTTGGCTGC"
     primer_bind     702..718
                     /label=7652
                     /note="color: black; sequence: GCTTTGGCTGCTCCAGC"
     primer_bind     complement(722..731)
                     /label=6221
                     /note="color: black; sequence: 
                     ctttcatataTCAGTGGTGGTGGTGGTG"
     primer_bind     complement(722..731)
                     /label=6176
                     /note="color: black; sequence: 
                     aagaaattcgTCAGTGGTGGTGGTGGTG"
     primer_bind     complement(722..731)
                     /label=3213
                     /note="color: black; sequence: 
                     ctcgaattcaTCAGTGGTGGTGGTGGTG"
     primer_bind     complement(722..731)
                     /label=2289
                     /note="color: black; sequence: 
                     AGAAATTCGCCTCGAGTCAGTGGTGGTGGTGGTG"
     primer_bind     849..861
                     /label=1729
                     /note="color: black; sequence: 
                     cttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1727
                     /note="color: black; sequence: 
                     gttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1737
                     /note="color: black; sequence: 
                     gtacttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1735
                     /note="color: black; sequence: 
                     gtagttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1733
                     /note="color: black; sequence: 
                     cttactactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1731
                     /note="color: black; sequence: 
                     cttagtactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     849..861
                     /label=1741
                     /note="color: black; sequence: 
                     gatgggggctactactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     849..861
                     /label=1739
                     /note="color: black; sequence: 
                     gatgggggctagtactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     complement(881..911)
                     /label=7241
                     /note="color: black; sequence: 
                     TTCTCTCTTGTCCAATTGAACACCTTCTTCC"
     primer_bind     complement(899..928)
                     /label=6992
                     /note="color: black; sequence: 
                     CCTGCAGCTGCACCTGTGCGTCAGCTTCTCTCTTGTCC"
     primer_bind     912..950
                     /label=6668
                     /note="color: black; sequence: 
                     ATATATGCTAGCGCTGACGCACAGGTGCAGCTGCAGGAAAGCGGC"
     primer_bind     912..949
                     /label=6993
                     /note="color: black; sequence: 
                     CTGCTAGCGCTGACGCACAGGTGCAGCTGCAGGAAAGCGG"
     misc_feature    921..1310
                     /label=AT110 nanobody
                     /note="color: #a6acb3"
     misc_feature    929..934
                     /label=BseR1
                     /note="color: #ff6600"
     primer_bind     935..951
                     /label=6949
                     /note="color: black; sequence: GCAGCTGCAGGAAAGCGGCG"
     primer_bind     complement(947..956)
                     /label=4390
                     /note="color: black; sequence: 
                     AATGTCAAGACCTGCCAGTTTGAGCGCGAGCATTTCCTTCCTGCCGGCGCCG"
     primer_bind     complement(949..958)
                     /label=4775
                     /note="color: black; sequence: 
                     CATGCATTTGTAGGCTTGATGCTTTTGGATCCATTAGGCGGCCGC"
     primer_bind     complement(970..979)
                     /label=4775
                     /note="color: black; sequence: 
                     CATGCATTTGTAGGCTTGATGCTTTTGGATCCATTAGGCGGCCGC"
     primer_bind     991..1039
                     /label=6978
                     /note="color: black; sequence: 
                     GCGCGGCGAGCGGCAATATTTTTGACGCTGACATCATGGGCTGGTATCG"
     primer_bind     complement(991..1039)
                     /label=6975
                     /note="color: black; sequence: 
                     CGATACCAGCCCATGATGTCAGCGTCAAAAATATTGCCGCTCGCCGCGC"
     primer_bind     991..1030
                     /label=6850
                     /note="color: black; sequence: 
                     GCGCGGCGAGCGGCAATATTTTTGACGTTGACATCATGGG"
     primer_bind     complement(991..1030)
                     /label=6849
                     /note="color: black; sequence: 
                     CCCATGATGTCAACGTCAAAAATATTGCCGCTCGCCGCGC"
     primer_bind     complement(991..1002)
                     /label=6702
                     /note="color: black; sequence: aaggatcaggacgctcgctgcgc"
     primer_bind     complement(1253..1292)
                     /label=6974
                     /note="color: black; sequence: 
                     CACCTGGGTGCCCTGGCCCCAATAGAAATAGTCAGAGTCG"
     primer_bind     1253..1292
                     /label=6973
                     /note="color: black; sequence: 
                     CGACTCTGACTATTTCTATTGGGGCCAGGGCACCCAGGTG"
     primer_bind     complement(1253..1292)
                     /label=6848
                     /note="color: black; sequence: 
                     CACCTGGGTGCCCTGGCCCCAATAGAAATTGTCAGAGTCG"
     primer_bind     1253..1292
                     /label=6847
                     /note="color: black; sequence: 
                     CGACTCTGACAATTTCTATTGGGGCCAGGGCACCCAGGTG"
     primer_bind     complement(1275..1300)
                     /label=6997
                     /note="color: black; sequence: 
                     TTTTtcatgAGCTGCTCACGGTCACCTGGGTGCCCTGGCC"
     primer_bind     1275..1292
                     /label=5646
                     /note="color: black; sequence: TCGGCCAGGGCACCAAGGTG"
     primer_bind     complement(1282..1300)
                     /label=6995
                     /note="color: black; sequence: 
                     gaattctcgcttcatgAGCTGCTCACGGTCACCTGGGTGC"
     primer_bind     complement(1285..1300)
                     /label=6948
                     /note="color: black; sequence: GCTGCTCACGGTCACCTGGG"
     primer_bind     complement(1285..1294)
                     /label=7631
                     /note="color: black; sequence: 
                     cgcgaattctcgcttcatgAGGGATGATACAGTCACCTGGG"
     primer_bind     complement(1285..1294)
                     /label=7580
                     /note="color: black; sequence: 
                     gcgaattctcgcttcatgAGttaGGATGATACAGTCACCTGGG"
     primer_bind     complement(1285..1294)
                     /label=7643
                     /note="color: black; sequence: 
                     GTCTTCTTCAGAAATAAGCTTTTGTGGATGATACAGTCACCTGGG"
     primer_bind     complement(1287..1316)
                     /label=6990
                     /note="color: black; sequence: 
                     GAACATCGTATGGGTAGGATCCGCTGCTCACGGTCACCTG"
     misc_feature    1300..1305
                     /label=BseR1
                     /note="color: #ff6600"
     primer_bind     1306..1362
                     /label=6989
                     /note="color: black; sequence: 
                     CAGGTGACCGTGAGCAGCGGATCCTACCCATACGATGTTC"
     misc_feature    1337..1346
                     /label=Factor Xa
                     /note="Factor Xa"
                     /note="color: #80ffff"
     misc_feature    1347..1373
                     /label=HA tag
                     /note="HA tag"
                     /note="color: #8080ff"
     primer_bind     1347..1366
                     /label=4377
                     /note="color: black; sequence: 
                     GGCGCTGTAAGGATCCTACCCATACGATGTTCCAGA"
     primer_bind     1347..1365
                     /label=7242
                     /note="color: black; sequence: GGATCCTACCCATACGATGTTCCAG"
     primer_bind     complement(1355..1373)
                     /label=6670
                     /note="color: black; sequence: 
                     TAGGATCCTCATGAAGCGTAATCTGGAACATCG"
     primer_bind     complement(1371..1391)
                     /label=4042
                     /note="color: black; sequence: accaccactagcctgcagagc"
     primer_bind     complement(1372..1392)
                     /label=2662
                     /note="color: black; sequence: 
                     CGGCTGACCCTAAGTGGGAAGCGCATTCTTCCATCTCGTCACCACCACTAGCCTGCAGA
                     G"
     primer_bind     complement(1372..1391)
                     /label=2958
                     /note="color: black; sequence: 
                     CCCACCTTCTGGTACCAGAGTGGAAATATAGGTTTTCAGTaccaccactagcctgcaga
                     g"
     primer_bind     complement(1383..1397)
                     /label=2047
                     /note="color: black; sequence: 
                     TGGCGGAAAACACCACCACCACCACCACT"
     primer_bind     complement(1383..1397)
                     /label=1965
                     /note="color: black; sequence: 
                     TGGCGGAAAACACCACCACCACCACCACT"
     primer_bind     complement(1384..1398)
                     /label=1477
                     /note="color: black; sequence: 
                     aatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1384..1398)
                     /label=LSE_T2_D1
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1384..1398)
                     /label=LSE_T2_C7
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1384..1398)
                     /label=1757
                     /note="color: black; sequence: 
                     tcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1384..1397)
                     /label=1775
                     /note="color: black; sequence: 
                     CAAAGCCACCCACCACCACCACCACCAC"
     primer_bind     complement(1384..1397)
                     /label=1941
                     /note="color: black; sequence: 
                     tcgtgttggatccTTCCAAACTACCACCACCACCAC"
     primer_bind     complement(1384..1397)
                     /label=1937
                     /note="color: black; sequence: 
                     agagaagtcgttcttagcgatgttaatcgtgttcataTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     complement(1384..1397)
                     /label=1795
                     /note="color: black; sequence: 
                     tgtcagagaagtcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     1384..1395
                     /label=6221
                     /note="color: black; sequence: 
                     ctttcatataTCAGTGGTGGTGGTGGTG"
     primer_bind     1384..1395
                     /label=6176
                     /note="color: black; sequence: 
                     aagaaattcgTCAGTGGTGGTGGTGGTG"
     primer_bind     1384..1395
                     /label=3213
                     /note="color: black; sequence: 
                     ctcgaattcaTCAGTGGTGGTGGTGGTG"
     primer_bind     1384..1395
                     /label=2289
                     /note="color: black; sequence: 
                     AGAAATTCGCCTCGAGTCAGTGGTGGTGGTGGTG"
     primer_bind     complement(1385..1405)
                     /label=1729
                     /note="color: black; sequence: 
                     cttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1727
                     /note="color: black; sequence: 
                     gttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1737
                     /note="color: black; sequence: 
                     gtacttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1735
                     /note="color: black; sequence: 
                     gtagttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1733
                     /note="color: black; sequence: 
                     cttactactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1731
                     /note="color: black; sequence: 
                     cttagtactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1385..1405)
                     /label=1741
                     /note="color: black; sequence: 
                     gatgggggctactactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     complement(1385..1405)
                     /label=1739
                     /note="color: black; sequence: 
                     gatgggggctagtactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     misc_feature    1386..1430
                     /label=(G4S)3
                     /note="color: #ffc0cb"
     primer_bind     1386..1407
                     /label=3104
                     /note="color: black; sequence: 
                     CTTAGGGTGcTTTGCCAAGGGTACCAATGTTTTAATGgGGTGGAGGTGGTTCTGGAGGT
                     G"
     primer_bind     complement(1387..1408)
                     /label=1775
                     /note="color: black; sequence: 
                     CAAAGCCACCCACCACCACCACCACCAC"
     primer_bind     complement(1387..1397)
                     /label=1477
                     /note="color: black; sequence: 
                     aatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1387..1397)
                     /label=LSE_T2_D1
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1387..1397)
                     /label=LSE_T2_C7
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1387..1397)
                     /label=1757
                     /note="color: black; sequence: 
                     tcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1388..1405)
                     /label=1316
                     /note="color: black; sequence: 
                     GGTATAGGATCCACCTGAACCCCCGCCGGAACCACCTCCA"
     primer_bind     1389..1410
                     /label=2430
                     /note="color: black; sequence: 
                     CGTCGTCTACGTTTTCTCCAAGAGAGGatccGGTGGTGGTGGTAGTGGTG"
     primer_bind     complement(1389..1401)
                     /label=986
                     /note="color: black; sequence: GCTAGCAGAACCACCACC"
     primer_bind     complement(1389..1401)
                     /label=7391
                     /note="color: black; sequence: 
                     CGCCACTTTCGACCAACTGTACCTCGCTAGCAGAACCACCACC"
     primer_bind     complement(1390..1401)
                     /label=1193
                     /note="color: black; sequence: gctgcacctgGCTAGCAGAACCACCAC"
     primer_bind     complement(1390..1401)
                     /label=1163
                     /note="color: black; sequence: 
                     CTCGCTCACATGCATGCTAGCAGAACCACCAC"
     primer_bind     complement(1392..1403)
                     /label=7049
                     /note="color: black; sequence: ctgaagtaccagaaccacc"
     primer_bind     complement(1395..1411)
                     /label=3071
                     /note="color: black; sequence: ACTTCCTCCACCTCCAGAACC"
     primer_bind     complement(1396..1416)
                     /label=6909
                     /note="color: black; sequence: 
                     caccagctgcacctgGCTAGCAGAACCACCACCACCAGAAC"
     primer_bind     complement(1397..1417)
                     /label=6600
                     /note="color: black; sequence: 
                     tgcggatccAGAACCTCCACCACCAGAA"
     primer_bind     complement(1397..1417)
                     /label=6597
                     /note="color: black; sequence: 
                     catggatccAGAACCACCACCTCCAGAA"
     primer_bind     complement(1400..1420)
                     /label=1316
                     /note="color: black; sequence: 
                     GGTATAGGATCCACCTGAACCCCCGCCGGAACCACCTCCA"
     primer_bind     complement(1400..1420)
                     /label=1729
                     /note="color: black; sequence: 
                     cttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1727
                     /note="color: black; sequence: 
                     gttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1737
                     /note="color: black; sequence: 
                     gtacttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1735
                     /note="color: black; sequence: 
                     gtagttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1733
                     /note="color: black; sequence: 
                     cttactactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1731
                     /note="color: black; sequence: 
                     cttagtactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1400..1420)
                     /label=1741
                     /note="color: black; sequence: 
                     gatgggggctactactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     complement(1400..1420)
                     /label=1739
                     /note="color: black; sequence: 
                     gatgggggctagtactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     1400..1410
                     /label=6221
                     /note="color: black; sequence: 
                     ctttcatataTCAGTGGTGGTGGTGGTG"
     primer_bind     1400..1410
                     /label=6176
                     /note="color: black; sequence: 
                     aagaaattcgTCAGTGGTGGTGGTGGTG"
     primer_bind     1400..1410
                     /label=3213
                     /note="color: black; sequence: 
                     ctcgaattcaTCAGTGGTGGTGGTGGTG"
     primer_bind     1400..1410
                     /label=2289
                     /note="color: black; sequence: 
                     AGAAATTCGCCTCGAGTCAGTGGTGGTGGTGGTG"
     primer_bind     1401..1422
                     /label=3104
                     /note="color: black; sequence: 
                     CTTAGGGTGcTTTGCCAAGGGTACCAATGTTTTAATGgGGTGGAGGTGGTTCTGGAGGT
                     G"
     primer_bind     complement(1402..1423)
                     /label=1775
                     /note="color: black; sequence: 
                     CAAAGCCACCCACCACCACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=1477
                     /note="color: black; sequence: 
                     aatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=LSE_T2_D1
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=LSE_T2_C7
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=1941
                     /note="color: black; sequence: 
                     tcgtgttggatccTTCCAAACTACCACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=1757
                     /note="color: black; sequence: 
                     tcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1402..1412)
                     /label=1937
                     /note="color: black; sequence: 
                     agagaagtcgttcttagcgatgttaatcgtgttcataTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     complement(1402..1412)
                     /label=1795
                     /note="color: black; sequence: 
                     tgtcagagaagtcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     complement(1403..1424)
                     /label=1316
                     /note="color: black; sequence: 
                     GGTATAGGATCCACCTGAACCCCCGCCGGAACCACCTCCA"
     primer_bind     1404..1425
                     /label=2430
                     /note="color: black; sequence: 
                     CGTCGTCTACGTTTTCTCCAAGAGAGGatccGGTGGTGGTGGTAGTGGTG"
     primer_bind     complement(1404..1416)
                     /label=986
                     /note="color: black; sequence: GCTAGCAGAACCACCACC"
     primer_bind     complement(1404..1416)
                     /label=7391
                     /note="color: black; sequence: 
                     CGCCACTTTCGACCAACTGTACCTCGCTAGCAGAACCACCACC"
     primer_bind     complement(1405..1416)
                     /label=1193
                     /note="color: black; sequence: gctgcacctgGCTAGCAGAACCACCAC"
     primer_bind     complement(1405..1416)
                     /label=1163
                     /note="color: black; sequence: 
                     CTCGCTCACATGCATGCTAGCAGAACCACCAC"
     primer_bind     complement(1407..1418)
                     /label=7049
                     /note="color: black; sequence: ctgaagtaccagaaccacc"
     primer_bind     complement(1410..1426)
                     /label=3071
                     /note="color: black; sequence: ACTTCCTCCACCTCCAGAACC"
     primer_bind     complement(1411..1436)
                     /label=6909
                     /note="color: black; sequence: 
                     caccagctgcacctgGCTAGCAGAACCACCACCACCAGAAC"
     primer_bind     complement(1412..1431)
                     /label=6600
                     /note="color: black; sequence: 
                     tgcggatccAGAACCTCCACCACCAGAA"
     primer_bind     complement(1412..1431)
                     /label=6597
                     /note="color: black; sequence: 
                     catggatccAGAACCACCACCTCCAGAA"
     primer_bind     1414..1435
                     /label=7667
                     /note="color: black; sequence: CTGGTGGTGGTGGTTCTGCTAG"
     primer_bind     complement(1415..1427)
                     /label=1729
                     /note="color: black; sequence: 
                     cttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1727
                     /note="color: black; sequence: 
                     gttttctggggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1737
                     /note="color: black; sequence: 
                     gtacttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1735
                     /note="color: black; sequence: 
                     gtagttttctgcggatatggatggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1733
                     /note="color: black; sequence: 
                     cttactactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1731
                     /note="color: black; sequence: 
                     cttagtactacgatatggatgtcggtggcggctccCACCATCACCATCACCACCA"
     primer_bind     complement(1415..1427)
                     /label=1741
                     /note="color: black; sequence: 
                     gatgggggctactactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     complement(1415..1427)
                     /label=1739
                     /note="color: black; sequence: 
                     gatgggggctagtactacgatatggatggtggcggctccCACCATCACCATCACCACCA
                     "
     primer_bind     1415..1425
                     /label=6221
                     /note="color: black; sequence: 
                     ctttcatataTCAGTGGTGGTGGTGGTG"
     primer_bind     1415..1425
                     /label=6176
                     /note="color: black; sequence: 
                     aagaaattcgTCAGTGGTGGTGGTGGTG"
     primer_bind     1415..1425
                     /label=3213
                     /note="color: black; sequence: 
                     ctcgaattcaTCAGTGGTGGTGGTGGTG"
     primer_bind     1415..1425
                     /label=2289
                     /note="color: black; sequence: 
                     AGAAATTCGCCTCGAGTCAGTGGTGGTGGTGGTG"
     primer_bind     complement(1417..1427)
                     /label=1775
                     /note="color: black; sequence: 
                     CAAAGCCACCCACCACCACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=1477
                     /note="color: black; sequence: 
                     aatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=LSE_T2_D1
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=LSE_T2_C7
                     /note="color: black; sequence: 
                     taatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=1941
                     /note="color: black; sequence: 
                     tcgtgttggatccTTCCAAACTACCACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=1757
                     /note="color: black; sequence: 
                     tcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCAC"
     primer_bind     complement(1417..1427)
                     /label=1937
                     /note="color: black; sequence: 
                     agagaagtcgttcttagcgatgttaatcgtgttcataTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     complement(1417..1427)
                     /label=1795
                     /note="color: black; sequence: 
                     tgtcagagaagtcgttcttagcgatgttaatcgtgttTTCCAAACTACCACCACCACCA
                     C"
     primer_bind     complement(1419..1438)
                     /label=7391
                     /note="color: black; sequence: 
                     CGCCACTTTCGACCAACTGTACCTCGCTAGCAGAACCACCACC"
     primer_bind     complement(1419..1436)
                     /label=986
                     /note="color: black; sequence: GCTAGCAGAACCACCACC"
     primer_bind     complement(1420..1436)
                     /label=1193
                     /note="color: black; sequence: gctgcacctgGCTAGCAGAACCACCAC"
     primer_bind     complement(1420..1436)
                     /label=1163
                     /note="color: black; sequence: 
                     CTCGCTCACATGCATGCTAGCAGAACCACCAC"
     primer_bind     complement(1422..1431)
                     /label=7049
                     /note="color: black; sequence: ctgaagtaccagaaccacc"
     primer_bind     complement(1425..1441)
                     /label=7415
                     /note="color: black; sequence: acgtcgctagcagaacc"
     primer_bind     complement(1425..1437)
                     /label=7629
                     /note="color: black; sequence: 
                     CGACAAGTTGGACTTCTGCGTCAGCGCTAGCAGAACC"
     primer_bind     complement(1425..1436)
                     /label=7582
                     /note="color: black; sequence: ATCTGgctagcagaacc"
     primer_bind     1431..1442
                     /label=5874
                     /note="color: black; sequence: 
                     CCTTTAAAGCCTGCTTTTTTGTACAGCTAGCGACGTC"
     misc_feature    1443..1649
                     /label=Aga2p
                     /note="color: #a6acb3"
     primer_bind     1472..1483
                     /label=932
                     /note="color: black; sequence: ggttctcacccctcaacaac"
     misc_feature    1593..1612
                     /label=primer
                     /note="color: #ff0000"
     primer_bind     1593..1612
                     /label=932
                     /note="color: black; sequence: ggttctcacccctcaacaac"
     misc_feature    1669..1743
                     /label=A75
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1744..1795
                     /label=Ribozyme
                     /note="color: #00ff00; direction: RIGHT"
     primer_bind     complement(1749..1779)
                     /label=LSE_T2_B4
                     /note="color: black; sequence: 
                     GGACTCATCACACCGGAAAACACATCCGGTG"
     primer_bind     1759..1789
                     /label=LSE_T2_B3
                     /note="color: black; sequence: 
                     GTTTTCCGGTGTGATGAGTCCGTGAGGACGA"
     primer_bind     1769..1785
                     /label=5548
                     /note="color: black; sequence: 
                     ACGAAACACCgCGACTACTGATGAGTCCGTGAGG"
     primer_bind     1769..1782
                     /label=4057
                     /note="color: black; sequence: 
                     CAAAAGATCTATGTGAGACCAGcgactactgatgagtccgtg"
     primer_bind     1787..1830
                     /label=2213
                     /note="color: black; sequence: 
                     ATCCtcatgagagacgCGAAACAGGgaattcgcgaatttcttatgatttatgattttta
                     t"
     primer_bind     1795..1821
                     /label=1647
                     /note="color: black; sequence: 
                     actgGAATTCgcgaatttcttatgatttat"
     primer_bind     1796..1841
                     /label=1748
                     /note="color: black; sequence: 
                     TTGtcatgaagcgagaattcgcgaatttcttatgatttatgatttttattattaaataa
                     g"
     primer_bind     1796..1826
                     /label=1289
                     /note="color: black; sequence: 
                     agctcatagacatgtaaaGGATCCATGCCGAATTCGCGaatttcttatgatttatgatt
                     t"
     primer_bind     1796..1815
                     /label=6237
                     /note="color: black; sequence: gaattcgcgaatttcttatg"
     primer_bind     1796..1810
                     /label=6812
                     /note="color: black; sequence: 
                     CATCTAAtcatgaagcgagcatgaagcgagaattcgcgaatttc"
     primer_bind     1797..1822
                     /label=6280
                     /note="color: black; sequence: 
                     catgtgagacggaggttcgtctccaattcgcgaatttcttatgatttatg"
     primer_bind     complement(1802..1842)
                     /label=2011
                     /note="color: black; sequence: 
                     ACTTATTTAATAATAAAAATCATAAATCATAAGAAATTCGC"
     primer_bind     1802..1842
                     /label=1586
                     /note="color: black; sequence: 
                     GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGT"
     primer_bind     1802..1842
                     /label=1671
                     /note="color: black; sequence: 
                     CTCAGATAGGCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGT"
     primer_bind     1802..1842
                     /label=7521
                     /note="color: black; sequence: 
                     gacaagtaactcgagGCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGT"
     primer_bind     1802..1842
                     /label=7517
                     /note="color: black; sequence: 
                     ttgccataactcgagGCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGT"
     primer_bind     complement(1802..1842)
                     /label=7420
                     /note="color: black; sequence: 
                     GACGTGTGCTCTTCCGATCnnnnnnnnnnnnnnnnnnnncataaatcataagaaattcg
                     c"
     primer_bind     1802..1841
                     /label=1682
                     /note="color: black; sequence: 
                     GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAG"
     primer_bind     1802..1841
                     /label=2361
                     /note="color: black; sequence: 
                     TGTACAAGtaaCTCGAGGcgaatttcttatgatttatgatttttattattaaataag"
     primer_bind     1802..1840
                     /label=1582
                     /note="color: black; sequence: 
                     tgtctgatttgctggaaataaGCGAATTTCTTATGATTTATGATTTTTATTATTAAATA
                     A"
     primer_bind     1802..1834
                     /label=2281
                     /note="color: black; sequence: 
                     CACCACCACCACCACCACTGACTCGAGGCGAATTTCTTATGATTTATGATTTTTATTAT
                     T"
     primer_bind     1802..1829
                     /label=83
                     /note="color: black; sequence: 
                     AGGCAGCTAAGAAAGCGAATTTCTTATGATTTATGATTTTTA"
     primer_bind     1802..1828
                     /label=4547
                     /note="color: black; sequence: 
                     ggcatcaccatcatcatcacggttaaggcgaatttcttatgatttatgattttt"
     primer_bind     1802..1826
                     /label=5858
                     /note="color: black; sequence: 
                     CTACATGAGCATGCCCTGCCCCTAAgcgaatttcttatgatttatgattt"
     primer_bind     1802..1822
                     /label=6124
                     /note="color: black; sequence: GCGAATTTCTTATGATTTATG"
     primer_bind     1802..1822
                     /label=3507
                     /note="color: black; sequence: 
                     caagcagacttgaCTCGAGGCGAATTTCTTATGATTTATG"
     primer_bind     1802..1822
                     /label=4059
                     /note="color: black; sequence: 
                     GCATGGCGAATGGGACCCCGGGgaggcgaatttcttatgatttatg"
     primer_bind     1802..1822
                     /label=4514
                     /note="color: black; sequence: 
                     gtatggatgaactgtacaaatgactgcagGCGAATTTCTTATGATTTATG"
     primer_bind     1802..1822
                     /label=7648
                     /note="color: black; sequence: 
                     CCAGTTTACCTTCTTCGATTTTCATCTCGAGGCGAATTTCTTATGATTTATG"
     primer_bind     1802..1822
                     /label=6279
                     /note="color: black; sequence: 
                     tcctcatgtgagacggaggttcgtctccaattgctcgaggcgaatttcttatgatttat
                     g"
     primer_bind     1802..1822
                     /label=6273
                     /note="color: black; sequence: 
                     tcctcatgtgagacggaggttcgtctccaattcctcgaggcgaatttcttatgatttat
                     g"
     primer_bind     1802..1820
                     /label=187
                     /note="color: black; sequence: CTCGAGGCGAATTTCTTATGATTTA"
     primer_bind     1802..1815
                     /label=6027
                     /note="color: black; sequence: CTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=2286
                     /note="color: black; sequence: CTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=1089
                     /note="color: black; sequence: CTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=934
                     /note="color: black; sequence: CTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=2197
                     /note="color: black; sequence: taaCTCGAGGcgaatttcttatg"
     primer_bind     1802..1815
                     /label=2169
                     /note="color: black; sequence: taactcgaggcgaatttcttatg"
     primer_bind     1802..1815
                     /label=1202
                     /note="color: black; sequence: TAACTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=1708
                     /note="color: black; sequence: 
                     tcggaGAATTCtaactcgaggcgaatttcttatg"
     primer_bind     1802..1815
                     /label=1161
                     /note="color: black; sequence: 
                     GTAATTTTCAGTTAACTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=7212
                     /note="color: black; sequence: 
                     CTGTTAAgGCAACATAACTCGAGGcgaatttcttatg"
     primer_bind     1802..1815
                     /label=2045
                     /note="color: black; sequence: 
                     tcaaagccACTAGTTAACTCGAGGCGAATTTCTTATG"
     primer_bind     1802..1815
                     /label=2338
                     /note="color: black; sequence: 
                     CATGGCATGGATGAGCTCTACAAATAACTCGAGGCGAATTTCTTATG"
     misc_feature    complement(1803..1967)
                     /label=T ADH1
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     1803..1857
                     /label=1776
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAG"
     primer_bind     1803..1841
                     /label=1781
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAATAAG"
     primer_bind     1803..1841
                     /label=6247
                     /note="color: black; sequence: 
                     ATCTGCCTAAcgaatttcttatgatttatgatttttattattaaataag"
     primer_bind     1803..1841
                     /label=6177
                     /note="color: black; sequence: 
                     CCACCACTGAcgaatttcttatgatttatgatttttattattaaataag"
     primer_bind     1803..1841
                     /label=6681
                     /note="color: black; sequence: 
                     tagaagaaaacaaaaaacaaacgaatttcttatgatttatgatttttattattaaataa
                     g"
     primer_bind     1803..1838
                     /label=298
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAAT"
     primer_bind     1803..1828
                     /label=1066
                     /note="color: black; sequence: 
                     ggttctggtcatcaccatcaccatcattaacgaatttcttatgatttatgattttt"
     primer_bind     1803..1822
                     /label=6281
                     /note="color: black; sequence: 
                     catgtgagacggaggttcgtctccaattgccgaatttcttatgatttatg"
     primer_bind     complement(1832..1856)
                     /label=67
                     /note="color: black; sequence: TTATTTTTTTTATAACTTATTTAAT"
     primer_bind     1866..1883
                     /label=L4440 vector, forward primer
                     /label=L4440
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(1875..1909)
                     /label=2875
                     /note="color: black; sequence: 
                     gaataagaattttcgttttaaaacctaagagtcac"
     primer_bind     complement(1918..1940)
                     /label=2344
                     /note="color: black; sequence: GCAACCTGACCTACAGGAAAGAG"
     primer_bind     complement(1936..1967)
                     /label=2756
                     /note="color: black; sequence: 
                     gcttgatatcgaattcctgcagcccggggagcgacctcatgctatacctgagaaagcaa
                     c"
     primer_bind     complement(1939..1967)
                     /label=1743
                     /note="color: black; sequence: 
                     gtaccacgcgtccatggactagtgagcgacctcatgctatacctgagaaagc"
     primer_bind     complement(1939..1967)
                     /label=2806
                     /note="color: black; sequence: 
                     gacccggcggggacgaggcaagctgagcgacctcatgctatacctgagaaagc"
     primer_bind     complement(1941..1967)
                     /label=7209
                     /note="color: black; sequence: 
                     gtaagaatttttgaaaattcaacatggactagtgagcgacctcatgctatacctgagaa
                     a"
     primer_bind     complement(1944..1967)
                     /label=3651
                     /note="color: black; sequence: gagcgacctcatgctatacctgag"
     primer_bind     complement(1947..1967)
                     /label=295
                     /note="color: black; sequence: 
                     gcacactagtGAGCGACCTCATGCTATACCT"
     primer_bind     complement(1947..1967)
                     /label=293
                     /note="color: black; sequence: 
                     gcacactagtGAGCGACCTCATGCTATACCT"
     primer_bind     complement(1948..1973)
                     /label=44
                     /note="color: black; sequence: 
                     ggccgagctcgagcgacctcatgctatacc"
     primer_bind     complement(1948..1968)
                     /label=2185
                     /note="color: black; sequence: 
                     gatcggtctcaCAGCgagcgacctcatgctatacc"
     primer_bind     complement(1948..1967)
                     /label=1176
                     /note="color: black; sequence: 
                     agccgtgatgaccctctagaGAGCGACCTCATGCTATACC"
     primer_bind     complement(1948..1967)
                     /label=LSE_T2_C9
                     /note="color: black; sequence: 
                     cggtaccacgcgtccatggactagtGAGCGACCTCATGCTATACC"
     primer_bind     complement(1948..1967)
                     /label=281
                     /note="color: black; sequence: 
                     TAAAGTTTATGTACAAATATCATAAAAAAAGAGAATCTTTGAGCGACCTCATGCTATAC
                     C"
     primer_bind     complement(1949..1967)
                     /label=7258
                     /note="color: black; sequence: 
                     cctttaataatttGAGCGACCTCATGCTATAC"
     primer_bind     complement(1949..1967)
                     /label=691
                     /note="color: black; sequence: 
                     ggtagaggtgtggtcaataaGAGCGACCTCATGCTATAC"
     primer_bind     complement(1949..1967)
                     /label=4548
                     /note="color: black; sequence: 
                     caacgcggccggtaccacgcgtccatggactagtgagcgacctcatgctatac"
     primer_bind     complement(1950..1972)
                     /label=721
                     /note="color: black; sequence: 
                     accgtgaaagctacttaaaagcttGAGCGACCTCATGCTATA"
     primer_bind     complement(1950..1968)
                     /label=6318
                     /note="color: black; sequence: 
                     aatcattgaattacaggaaaaaaaggcaaatatgattcatgcGAGCGACCTCATGCTAT
                     A"
     primer_bind     complement(1950..1967)
                     /label=6322
                     /note="color: black; sequence: 
                     tgtccgaattttcaatagggcgaacttgaagaataaccaaggGAGCGACCTCATGCTAT
                     A"
     primer_bind     complement(1950..1967)
                     /label=6320
                     /note="color: black; sequence: 
                     atcgaacgcaaaaaacattaactggaggctttagtaaaaatgGAGCGACCTCATGCTAT
                     A"
     primer_bind     complement(1954..1972)
                     /label=4673
                     /note="color: black; sequence: 
                     acccgagctcAAGCTTGAGCGACCTCATGC"
     primer_bind     complement(1954..1972)
                     /label=2602
                     /note="color: black; sequence: 
                     atttaatagaaaaAAGCTTGAGCGACCTCATGC"
     primer_bind     complement(1954..1972)
                     /label=872
                     /note="color: black; sequence: 
                     cagttattacccgagctcAAGCTTGAGCGACCTCATGC"
     primer_bind     complement(1954..1967)
                     /label=2046
                     /note="color: black; sequence: 
                     acgcggccggtaccacgcgtCCATGGGAGCGACCTCATGC"
     primer_bind     complement(1955..1972)
                     /label=3513
                     /note="color: black; sequence: 
                     gaatgattcttaataAAGCTTGAGCGACCTCATG"
     primer_bind     complement(1956..1972)
                     /label=3548
                     /note="color: black; sequence: 
                     gtaagatttaagtcAAGCTTGAGCGACCTCAT"
     primer_bind     complement(1956..1967)
                     /label=578
                     /note="color: black; sequence: 
                     GGTAGAGGTGTGGTCAATAAGAGCGACCTCAT"
     primer_bind     1958..2008
                     /label=1752
                     /note="color: black; sequence: 
                     GAGGTCGCTCGAGCTCCGTTTCTATTATGAATTTCATTTATAAAGTTTATG"
     primer_bind     complement(1958..1972)
                     /label=2550
                     /note="color: black; sequence: 
                     TATAAATGTCTTATATCGATTATTTaagcttgagcgacctc"
     primer_bind     1966..1994
                     /label=2
                     /note="color: black; sequence: 
                     GATCgagctcCGTTTCTATTATGAATTTCAT"
     primer_bind     1967..2002
                     /label=565
                     /note="color: black; sequence: 
                     cagttattacccgagctCCGTTTCTATTATGAATTTCATTTATAAAG"
     primer_bind     1967..1986
                     /label=3113
                     /note="color: black; sequence: acccgagctccgtttctattatg"
     primer_bind     1968..1992
                     /label=1182
                     /note="color: black; sequence: GAGCTCCGTTTCTATTATGAATTTC"
     primer_bind     1968..1992
                     /label=338
                     /note="color: black; sequence: 
                     aataattataaaGAGCTCCGTTTCTATTATGAATTTC"
     primer_bind     1974..2028
                     /label=LSE_T2_B6
                     /note="color: black; sequence: 
                     CGTTTCTATTATGAATTTCATTTATAAAGTTTATGTACAAATATCATAAAAAAAG"
     primer_bind     1974..2008
                     /label=6269
                     /note="color: black; sequence: 
                     CGTTTCTATTATGAATTTCATTTATAAAGTTTATG"
     primer_bind     1974..2008
                     /label=874
                     /note="color: black; sequence: 
                     CGTTTCTATTATGAATTTCATTTATAAAGTTTATG"
     primer_bind     1974..2008
                     /label=2021
                     /note="color: black; sequence: 
                     TTATAAATATACTATTTATGTAATTcgtttctattatgaatttcatttataaagtttat
                     g"
     primer_bind     1974..2002
                     /label=4675
                     /note="color: black; sequence: 
                     catcccgcggCGTTTCTATTATGAATTTCATTTATAAAG"
     primer_bind     complement(1992..2039)
                     /label=1288
                     /note="color: black; sequence: 
                     gcaaaactaaAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATG"
     primer_bind     complement(2002..2061)
                     /label=1744
                     /note="color: black; sequence: 
                     atttcgtcaaaaatgctaagaaaaaaagattctctttttttatgatatttgtacataaa
                     c"
     CDS             2003..2005
                     /codon_start=1
                     /label=pGKL1 ORF3 delta ORF2 delta
                     /note="color: #993366"
                     /translation="F"
     primer_bind     2006..2065
                     /label=1747
                     /note="color: black; sequence: 
                     atgtacaaatatcataaaaaaagagaatctttttttcttagcatttttgacgaaatttg
                     c"
     primer_bind     complement(2007..2037)
                     /label=1880
                     /note="color: black; sequence: 
                     GAGGGGATCCAAAGATTCTCTTTTTTTATGATATTTGTACA"
     primer_bind     complement(2008..2039)
                     /label=7664
                     /note="color: black; sequence: 
                     GAACTGGTTATAAGTACCACCGTATAAaaagattctctttttttatgatatttgtac"
     primer_bind     complement(2008..2038)
                     /label=2724
                     /note="color: black; sequence: 
                     TGGTGCATGACCCGCAAGCCCGGTGCCtgaaaagattctctttttttatgatatttgta
                     c"
     primer_bind     complement(2008..2037)
                     /label=663
                     /note="color: black; sequence: 
                     AAAGATTCTCTTTTTTTATGATATTTGTAC"
     primer_bind     complement(2008..2037)
                     /label=572
                     /note="color: black; sequence: 
                     tacaagatcAAAGATTCTCTTTTTTTATGATATTTGTAC"
     primer_bind     complement(2008..2037)
                     /label=3933
                     /note="color: black; sequence: 
                     gcacttgcctAAAGATTCTCTTTTTTTATGATATTTGTAC"
     primer_bind     complement(2008..2037)
                     /label=3795
                     /note="color: black; sequence: 
                     taagaaatagAAAGATTCTCTTTTTTTATGATATTTGTAC"
     primer_bind     2011..2056
                     /label=4926
                     /note="color: black; sequence: 
                     caaatatcataaaaaaagagaatctttCTATTTCTTAGCATTTTTGAC"
     primer_bind     complement(2011..2037)
                     /label=4923
                     /note="color: black; sequence: aaagattctctttttttatgatatttg"
     primer_bind     complement(2018..2037)
                     /label=7672
                     /note="color: black; sequence: 
                     ccatattaaattttatatctctggatccaaagattctctttttttatg"
     primer_bind     2028..2057
                     /label=3796
                     /note="color: black; sequence: 
                     gagaatctttCTATTTCTTAGCATTTTTGACG"
     CDS             2036
                     /codon_start=1
                     /label=pGKL1 ORF3 delta ORF2 delta
                     /note="color: #993366"
                     /translation=""
     misc_feature    complement(2037..2042)
                     /label=MluI
                     /note="color: #0080ff; direction: LEFT"
     misc_structure  complement(2039..2710)
                     /label=TRP1
                     /note="This reverse directional feature has 2 segments:
                        1: 2039 .. 2425 / #a6acb3
                        2: 2426 .. 2710 / #a6acb3"
     primer_bind     2039..2064
                     /label=3882
                     /note="color: black; sequence: 
                     TTATAATACAGTTTTTTAtttcttagcatttttgacgaaatttg"
     primer_bind     2039..2064
                     /label=3844
                     /note="color: black; sequence: 
                     gagctcgggtaataaCTATTTCTTAGCATTTTTGACGAAATTTG"
     primer_bind     2039..2064
                     /label=5905
                     /note="color: black; sequence: 
                     tataattattataaaaactttcatataCTATTTCTTAGCATTTTTGACGAAATTTG"
     primer_bind     2039..2064
                     /label=7407
                     /note="color: black; sequence: 
                     cttataggaggtagtatataaggtccttcttCTATTTCTTAGCATTTTTGACGAAATTT
                     G"
     primer_bind     2039..2058
                     /label=5265
                     /note="color: black; sequence: 
                     aggtctcgTCGAGTTAGTGATGATGGTGGTGATGGGATCCTTTCTTAGCATTTTTGACG
                     A"
     primer_bind     2039..2057
                     /label=3832
                     /note="color: black; sequence: 
                     ttataatacagttttCTATTTCTTAGCATTTTTGACG"
     primer_bind     2039..2056
                     /label=3808
                     /note="color: black; sequence: 
                     gatttattaatattaCTATTTCTTAGCATTTTTGAC"
     primer_bind     2039..2056
                     /label=4685
                     /note="color: black; sequence: 
                     attggtctggtctcaactctCTATTTCTTAGCATTTTTGAC"
     primer_bind     2367..2380
                     /label=3542
                     /note="color: black; sequence: AGTGAAGGCAAAACGAGGAA"
     primer_bind     2562..2580
                     /label=5122
                     /note="color: black; sequence: GGGTCAATTGTTCTCTTTC"
     primer_bind     2677..2695
                     /label=4748
                     /note="color: black; sequence: GACCAGAACTACCTGTGAA"
     primer_bind     complement(2680..2739)
                     /label=1746
                     /note="color: black; sequence: 
                     tatatgaaagtttttataataattataaaatgtctgttattaatttcacaggtagttct
                     g"
     primer_bind     complement(2685..2714)
                     /label=7406
                     /note="color: black; sequence: 
                     aaaCatGtgataagctcatagacatgtaaaATGTCTGTTATTAATTTCACAGGTAG"
     primer_bind     complement(2685..2714)
                     /label=5904
                     /note="color: black; sequence: 
                     aaatatatgataagctcatagacatgtaaaATGTCTGTTATTAATTTCACAGGTAG"
     primer_bind     complement(2688..2710)
                     /label=5264
                     /note="color: black; sequence: 
                     gctaggtctccgatctATGTCTGTTATTAATTTCACAGG"
     primer_bind     complement(2691..2738)
                     /label=4925
                     /note="color: black; sequence: 
                     atatgaaagtttttataataattataaaATGTCTGTTATTAATTTCAC"
     primer_bind     complement(2691..2710)
                     /label=4684
                     /note="color: black; sequence: 
                     gatattttgacaaacaaattgcatATGTCTGTTATTAATTTCAC"
     primer_bind     complement(2693..2739)
                     /label=3993
                     /note="color: black; sequence: 
                     tattaatttaaaaTATATGAAAGTTTTTATAATAATTATAAAATGTCTGTTATTAATTT
                     C"
     primer_bind     2702..2761
                     /label=6537
                     /note="color: black; sequence: 
                     aacagacattttataattattataaaaactttcatataagagatataaaatttaatatg
                     g"
     primer_bind     complement(2704..2739)
                     /label=3999
                     /note="color: black; sequence: 
                     tattaatttaaaaTATATGAAAGTTTTTATAATAATTATAAAATGTCTG"
     primer_bind     complement(2704..2739)
                     /label=5962
                     /note="color: black; sequence: 
                     gcggccagcaaaactaatatatgaaagtttttataataattataaaATGTCTG"
     primer_bind     complement(2704..2739)
                     /label=3807
                     /note="color: black; sequence: 
                     cgtaaatcatgagtatcggaattcTATATGAAAGTTTTTATAATAATTATAAAATGTCT
                     G"
     primer_bind     complement(2707..2739)
                     /label=5508
                     /note="color: black; sequence: 
                     agaagaatgattaaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2707..2739)
                     /label=5504
                     /note="color: black; sequence: 
                     ttcgtaaaaaataaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2707..2739)
                     /label=5500
                     /note="color: black; sequence: 
                     ttctcgagaagtaaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2707..2739)
                     /label=5496
                     /note="color: black; sequence: 
                     agctcatcccataaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2707..2739)
                     /label=5492
                     /note="color: black; sequence: 
                     caagagatttataaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2707..2739)
                     /label=5488
                     /note="color: black; sequence: 
                     gtgggagggggtaaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATGT
                     "
     primer_bind     complement(2708..2740)
                     /label=2555
                     /note="color: black; sequence: 
                     TATAAATGTCTTATATCGATTATTTtatatgaaagtttttataataattataaaatg"
     primer_bind     2708..2739
                     /label=91
                     /note="color: black; sequence: 
                     cagtatgcatTTTATAATTATTATAAAAACTTTCATATA"
     primer_bind     complement(2708..2739)
                     /label=788
                     /note="color: black; sequence: 
                     aagggccgcggtatatgaaagtttttataataattataaaatg"
     primer_bind     2708..2739
                     /label=9
                     /note="color: black; sequence: 
                     GTCAGGTACCATGCATTTTATAATTATTATAAAAACTTTCATATA"
     primer_bind     complement(2708..2739)
                     /label=6021
                     /note="color: black; sequence: 
                     gaaaaatgtaaGTATCGGAATTCTATATGAAAGTTTTTATAATAATTATAAAATG"
     primer_bind     2708..2734
                     /label=113
                     /note="color: black; sequence: 
                     tgcattttataattattataaaaactttc"
     5'UTR           complement(2711..2739)
                     /label=UTR region from pGKL1 ORF2
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(2715..2739)
                     /label=286
                     /note="color: black; sequence: 
                     TAATATATGAAAGTTTTTATAATAATTA"
     primer_bind     complement(2715..2739)
                     /label=355
                     /note="color: black; sequence: 
                     tattaatttaaaaTATATGAAAGTTTTTATAATAATTA"
     primer_bind     complement(2715..2739)
                     /label=463
                     /note="color: black; sequence: 
                     gaagtcatcaccaccatcatcacTAATATATGAAAGTTTTTATAATAATTA"
     primer_bind     complement(2720..2741)
                     /label=214
                     /note="color: black; sequence: 
                     ggagaaacatcccaaattaaattatactTATATGAAAGTTTTTATAAT"
     CDS             2740..2865
                     /codon_start=1
                     /label=Toxin subunit gamma
                     /label=Toxin subunit gamma (gene 4)
                     /note="color: #993366"
                     /translation="RDIKFNMEEIRQERYNQMKEALNSVEGYKGKIVASDSDWCFK"
     primer_bind     2740..2773
                     /label=7669
                     /note="color: black; sequence: 
                     cataaaaaaagagaatctttggatccagagatataaaatttaatatggaaaaaataaga
                     c"
     primer_bind     2740..2761
                     /label=804
                     /note="color: black; sequence: 
                     catgggatcccctagagatataaaatttaatatgg"
     primer_bind     2800..2819
                     /label=1916
                     /note="color: black; sequence: GCTCTAAATAGTGTTGAAGG"
     primer_bind     2837..2859
                     /label=6518
                     /note="color: black; sequence: 
                     GAAAGCTGTGCGATAACCATATCTGGAAAAGTTAGcctcagactcagattggtgt"
     primer_bind     2838..2859
                     /label=6517
                     /note="color: black; sequence: 
                     CATCGCATGTTTATTATAACTCTTCATTAATAAAGcctcagactcagattggtgt"
     primer_bind     2838..2859
                     /label=6516
                     /note="color: black; sequence: 
                     CGATGACAACGCTGTGTCTCTGAACGACTAGAGAGcctcagactcagattggtgt"
     primer_bind     2840..2890
                     /label=7659
                     /note="color: black; sequence: 
                     cccctcagactcagattggtgtTTCaaagatcctcaaggcaatagaataacag"
     primer_bind     2840..2884
                     /label=453
                     /note="color: black; sequence: 
                     cagtacgcgtcctcagactcagattggtgtaaagatcctcaaggcaatagaa"
     primer_bind     2840..2882
                     /label=7673
                     /note="color: black; sequence: 
                     cctcagactcagattggtgtttcaaagatcctcaaggcaatag"
     primer_bind     2840..2862
                     /label=5257
                     /note="color: black; sequence: cctcagactcagattggtgtttc"
     primer_bind     2840..2862
                     /label=6210
                     /note="color: black; sequence: 
                     tccttgcttaCCTCAGACTCAGATTGGTGTTTC"
     primer_bind     2840..2862
                     /label=6782
                     /note="color: black; sequence: 
                     agaagttaagaaaatccttgcttaAcctcagactcagattggtgtttc"
     primer_bind     complement(2840..2859)
                     /label=6852
                     /note="color: black; sequence: ACACCAATCTGAGTCTGAGG"
     primer_bind     complement(2840..2859)
                     /label=2815
                     /note="color: black; sequence: tctttacaccaatctgagtctgagg"
     primer_bind     2840..2859
                     /label=4431
                     /note="color: black; sequence: 
                     gcaaaactaacctcagactcagattggtgt"
     primer_bind     2840..2859
                     /label=6515
                     /note="color: black; sequence: 
                     GCCCTTCCCAATTAGCATTCGTTAATACTGGTATTCAACAcctcagactcagattggtg
                     t"
     primer_bind     2840..2859
                     /label=6514
                     /note="color: black; sequence: 
                     TCTTTTTCATGTAGTATCCAGCTCCGATTGAACCGAACGCcctcagactcagattggtg
                     t"
     primer_bind     2840..2858
                     /label=7199
                     /note="color: black; sequence: 
                     aatccttgcttaaCCTCAGACTCAGATTGGTG"
     primer_bind     2840..2853
                     /label=1946
                     /note="color: black; sequence: ACGCGTCCTCAGACTCAGAT"
     primer_bind     2840..2853
                     /label=568
                     /note="color: black; sequence: GGATCCCCTCAGACTCAGAT"
     primer_bind     2840..2853
                     /label=1879
                     /note="color: black; sequence: 
                     GAGAATCTTTGGATCCCCTCAGACTCAGAT"
     primer_bind     2840..2853
                     /label=581
                     /note="color: black; sequence: 
                     tatgtataggtcatcGGATCCCCTCAGACTCAGAT"
     primer_bind     2840..2853
                     /label=860
                     /note="color: black; sequence: 
                     tgtcaaattcaacttctaaattattcttaataatcttagtacgcgtcctcagactcaga
                     t"
     primer_bind     2840..2853
                     /label=858
                     /note="color: black; sequence: 
                     ctttttaataccatatttagaaattatatatctaaggtaaacgcgtcctcagactcaga
                     t"
     primer_bind     2840..2853
                     /label=857
                     /note="color: black; sequence: 
                     atgtgtataataaaatgacttataggtcaaaagtgtaaaaacgcgtcctcagactcaga
                     t"
     primer_bind     complement(2843..2890)
                     /label=7670
                     /note="color: black; sequence: 
                     ctgttattctattgccttgaggatctttgaaacaccaatctgagtctg"
     primer_bind     2862..2890
                     /label=5707
                     /note="color: black; sequence: 
                     agaggtctcagatccaaagatcctcaaggcaatagaataacag"
     primer_bind     complement(2865..2905)
                     /label=454
                     /note="color: black; sequence: 
                     atcttctagaagtactatcaaaatctgttattctattgccttgaggatct"
     CDS             2866..2901
                     /codon_start=1
                     /label=Toxin subunit gamma
                     /label=Toxin subunit gamma (gene 4)
                     /note="color: #993366"
                     /translation="DPQGNRITDFDS"
     primer_bind     complement(2872..2901)
                     /label=443
                     /note="color: black; sequence: 
                     ACTATCAAAATCTGTTATTCTATTGCCTTG"
     primer_bind     complement(2875..2922)
                     /label=7547
                     /note="color: black; sequence: 
                     gtgtcggggctggcttaaagtACTATCAAAATCTGTTATTCTATTGCC"
     primer_bind     2875..2922
                     /label=7546
                     /note="color: black; sequence: 
                     GGCAATAGAATAACAGATTTTGATAGTactttaagccagccccgacac"
     primer_bind     complement(2882..2905)
                     /label=1183
                     /note="color: black; sequence: 
                     aaataggggaattaaaaaaaTTCTAGAAGTACTATCAAAATCTGTTATTC"
     primer_bind     complement(2888..2905)
                     /label=4832
                     /note="color: black; sequence: 
                     gcgcaTCTAGAagtactatcaaaatctg"
     primer_bind     2906..2953
                     /label=4810
                     /note="color: black; sequence: 
                     GAGGAAAGTCTAAGCCAGCCCCCTGATGGG"
     primer_bind     2908..2953
                     /label=4795
                     /note="color: black; sequence: AGGAGGATGAGCCAGCCCCCTGATGGG"
     primer_bind     2909..2953
                     /label=4793
                     /note="color: black; sequence: 
                     GAGGAAGGTGtagGCCAGCCCCCTGATGGG"
     primer_bind     2909..2953
                     /label=2307
                     /note="color: black; sequence: 
                     aacaaaatagatattttgacaaacaaattgcatGCCAGCCCCCTGATGGG"
     primer_bind     2923..2953
                     /label=2827
                     /note="color: black; sequence: TACGggatccGCCAGCCCCCTGATGGG"
     primer_bind     complement(2969..3016)
                     /label=6263
                     /note="color: black; sequence: 
                     gacacatgcagctcccgcgtcacagcttgtctgtaagcggatg"
     primer_bind     complement(2973..2993)
                     /label=7398
                     /note="color: black; sequence: 
                     aagtatgacctaattgactccggcgaaaaagcatgcGTCACAGCTTGTCTGTAAGCG"
     primer_bind     2976..3020
                     /label=6264
                     /note="color: black; sequence: 
                     ttacagacaagctgtgacgcgggagctgcatgtgtcagag"
     primer_bind     2994..3009
                     /label=7403
                     /note="color: black; sequence: 
                     ttttaaattaataatgtaattcatatattatagtactCGTCTCCGGGAGCTGC"
     primer_bind     complement(3002..3023)
                     /label=1959
                     /note="color: black; sequence: AACCTCTGACACATGCAGCTCC"
     primer_bind     complement(3043..3082)
                     /label=AW_BB_141_R
                     /note="color: black; sequence: 
                     ctccttttggagcctttttttttgcctttcgtctcgcgcg"
     primer_bind     3043..3082
                     /label=AW_BLA_from12_F
                     /note="color: black; sequence: 
                     cgcgcgagacgaaaggcaaaaaaaaaggctccaaaaggag"
     CDS             complement(3100..3960)
                     /codon_start=1
                     /label=AmpR
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3127..3152)
                     /label=3831
                     /note="color: black; sequence: AACGAAATAGACAGATTGCTGAAATA"
     primer_bind     3127..3152
                     /label=3830
                     /note="color: black; sequence: tatttcagcaatctgtctatttcgtt"
     primer_bind     complement(3149..3171)
                     /label=6924
                     /note="color: black; sequence: GGAGTCAGGCAACTATGGATGAACG"
     primer_bind     complement(3199..3213)
                     /label=3584
                     /note="color: black; sequence: CTTCTGAGGATGGTAAGACTTCC"
     primer_bind     complement(3210..3229)
                     /label=1718
                     /note="color: black; sequence: tgccgcacttggaccagatg"
     primer_bind     3210..3229
                     /label=1717
                     /note="color: black; sequence: catctggtccaagtgcggca"
     primer_bind     complement(3252..3261)
                     /label=5331
                     /note="color: black; sequence: 
                     CTAGAGGTCGACGGgaattcgtagggaaggggcaggag"
     primer_bind     3254..3306
                     /label=3050
                     /note="color: black; sequence: 
                     CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAG"
     primer_bind     3309..3368
                     /label=3051
                     /note="color: black; sequence: 
                     GTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGA
                     G"
     primer_bind     complement(3314..3333)
                     /label=1532
                     /note="color: black; sequence: gaggcggataaagttgcagg"
     primer_bind     complement(3346..3368)
                     /label=4944
                     /note="color: black; sequence: ctctagcttcccggcaacaatta"
     primer_bind     complement(3346..3363)
                     /label=3943
                     /note="color: black; sequence: TAGCTTCCCGGCAACAATTA"
     primer_bind     3348..3371
                     /label=4945
                     /note="color: black; sequence: attgttgccgggaagctagagtaa"
     primer_bind     3369..3428
                     /label=3052
                     /note="color: black; sequence: 
                     TAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG
                     G"
     primer_bind     complement(3407..3417)
                     /label=1956
                     /note="color: black; sequence: AAGTGGAAGCGGCGATGGC"
     primer_bind     complement(3420..3449)
                     /label=6042
                     /note="color: black; sequence: 
                     taccaaacgaCGAGCGTGACACCACGATGC"
     primer_bind     complement(3420..3449)
                     /label=6036
                     /note="color: black; sequence: 
                     taccaaacgaCGAGCGTGACACCACGATGC"
     primer_bind     3434..3460
                     /label=6043
                     /note="color: black; sequence: 
                     gtcacgctcgTCGTTTGGTATGGCTTCATTC"
     primer_bind     3434..3460
                     /label=6037
                     /note="color: black; sequence: 
                     gtcacgctcgTCGTTTGGTATGGCTTCATTC"
     primer_bind     3438..3455
                     /label=3550
                     /note="color: black; sequence: CGTCGTTTGGTATGGCTT"
     primer_bind     complement(3441..3462)
                     /label=7051
                     /note="color: black; sequence: ggagctgaatgaagccataccaaacg"
     primer_bind     complement(3492..3521)
                     /label=6023
                     /note="color: black; sequence: 
                     ccgcttttttGCACAATATGGGAGACCATG"
     primer_bind     3502..3532
                     /label=6022
                     /note="color: black; sequence: 
                     catattgtgcAAAAAAGCGGTTAATTCCTTC"
     primer_bind     3540..3580
                     /label=3958
                     /note="color: black; sequence: 
                     cgatggttgtcagaagtaagTTCGCCGCAGTGTTATCACTC"
     primer_bind     complement(3540..3559)
                     /label=3957
                     /note="color: black; sequence: CTTACTTCTGACAACCATCG"
     primer_bind     complement(3541..3561)
                     /label=6293
                     /note="color: black; sequence: CCAACTTACTTCTGACAACGATC"
     primer_bind     complement(3624..3662)
                     /label=685
                     /note="color: black; sequence: 
                     tggttgagtatTCACCAGTCACAGAAAAGCATCTTACGG"
     primer_bind     complement(3626..3663)
                     /label=680
                     /note="color: black; sequence: 
                     ttggttgagcACTCACCAGTCACAGAAAAGCATCTTAC"
     primer_bind     complement(3654..3674)
                     /label=3551
                     /note="color: black; sequence: CGCAGAATGACTTGGTTGAAT"
     primer_bind     3656..3672
                     /label=686
                     /note="color: black; sequence: 
                     tgactggtgaaTACTCAACCAAGTCATTCTG"
     primer_bind     complement(3760..3780)
                     /label=5949
                     /note="color: black; sequence: gaagagcgttttccaatgatg"
     primer_bind     3760..3777
                     /label=5950
                     /note="color: black; sequence: catcattggaaaacgctc"
     primer_bind     3771..3790
                     /label=876
                     /note="color: black; sequence: AACGTTCTTCGGGGCGAAAA"
     primer_bind     3773..3785
                     /label=961
                     /note="color: black; sequence: CTGTTTCCGCTCTGCAGGGC"
     primer_bind     3858..3870
                     /label=4373
                     /note="color: black; sequence: gccgtcactgcgtcttttac"
     primer_bind     complement(3879..3891)
                     /label=1663
                     /note="color: black; sequence: 
                     ttacatagaaaataagactgtcaaagccaccGGATCCcacccagaaacgc"
     primer_bind     complement(3889..3908)
                     /label=3944
                     /note="color: black; sequence: GCCTTCCTGTTTTTGCTCAC"
     primer_bind     3925..3948
                     /label=4956
                     /note="color: black; sequence: 
                     tgatgcttttGGGAATAAGGGCGACACGGAAATG"
     primer_bind     complement(3935..3961)
                     /label=2648
                     /note="color: black; sequence: 
                     aaggggtgttatgagtattcaacatttccgtgtcgc"
     primer_bind     complement(3935..3961)
                     /label=2541
                     /note="color: black; sequence: 
                     aaggggtgttatgagtattcaacatttccgtgtcgc"
     primer_bind     3957..3988
                     /label=5692
                     /note="color: black; sequence: 
                     cttgttcaatcatactcttcctttttcaatattattgaagc"
     primer_bind     complement(3974..4033)
                     /label=1722
                     /note="color: black; sequence: 
                     acattcaaatatgtatccgctcatcagacaataaccctgataaatgcttcaataatatt
                     g"
     primer_bind     complement(3987..4016)
                     /label=1753
                     /note="color: black; sequence: 
                     CGCTCATCAGACAATAACCCTGATAAATGC"
     primer_bind     complement(3988..4027)
                     /label=3817
                     /note="color: black; sequence: 
                     aaatatgtatccgctgATGAGACAATAACCCTGATAAATG"
     primer_bind     3993..4015
                     /label=2866
                     /note="color: black; sequence: ATCAGGGTTATTGTCTCATGAGC"
     primer_bind     complement(3994..4015)
                     /label=3173
                     /note="color: black; sequence: GCTCATGAGACAATAACCCTGA"
     primer_bind     3994..4015
                     /label=3172
                     /note="color: black; sequence: TCAGGGTTATTGTCTCATGAGC"
     primer_bind     3995..4028
                     /label=1596
                     /note="color: black; sequence: 
                     cagggttattgtcTgATGAgcggatacatatttg"
     primer_bind     complement(3995..4028)
                     /label=1595
                     /note="color: black; sequence: 
                     caaatatgtatccgcTCATcAgacaataaccctg"
     primer_bind     3996..4055
                     /label=1721
                     /note="color: black; sequence: 
                     agggttattgtctgatgagcggatacatatttgaatgtatttagaaaaataaacaaata
                     g"
     primer_bind     3996..4017
                     /label=2920
                     /note="color: black; sequence: AGGGTTATTGTCTCATGAGCGG"
     primer_bind     3997..4039
                     /label=3816
                     /note="color: black; sequence: 
                     gggttattgtctcatcAGCGGATACATATTTGAATGTATTTAG"
     primer_bind     3997..4017
                     /label=4732
                     /note="color: black; sequence: GGGTTATTGTCTCATGAGCGG"
     primer_bind     3999..4028
                     /label=1754
                     /note="color: black; sequence: 
                     GTTATTGTCTGATGAGCGGATACATATTTG"
     primer_bind     4031..4050
                     /label=4273
                     /note="color: black; sequence: TGTATTTAGAAAAATAAACA"
     primer_bind     complement(4039..4069)
                     /label=1181
                     /note="color: black; sequence: 
                     TTTTTTTAATTCCCCTATTTGTTTATTTTTC"
     primer_bind     complement(4039..4058)
                     /label=5943
                     /note="color: black; sequence: cgcggaacccctatttgtttatttttc"
     primer_bind     complement(4047..4058)
                     /label=4498
                     /note="color: black; sequence: 
                     GAATGCTGGTCGCTATACTGcgcggaacccctatttgtt"
     primer_bind     4070..4089
                     /label=1173
                     /note="color: black; sequence: 
                     taatagaaacggagctcAGCCCGCTCATTAGGCGGGC"
     primer_bind     4070..4083
                     /label=LSE_T2_B8
                     /note="color: black; sequence: 
                     gaaattcataatagaaacgGAGCTCAGCCCGCTCATTAG"
     primer_bind     4091..4115
                     /label=1707
                     /note="color: black; sequence: 
                     actgCTCGAGactagtcggatatagttcctccttt"
     primer_bind     4091..4110
                     /label=1762
                     /note="color: black; sequence: gcacACTAGTcggatatagttcct"
     primer_bind     4092..4116
                     /label=1645
                     /note="color: black; sequence: CTAGTCGGATATAGTTCCTCCTTTC"
     primer_bind     4092..4111
                     /label=1628
                     /note="color: black; sequence: ctagtcggatatagttcctc"
     primer_bind     4099..4127
                     /label=1059
                     /note="color: black; sequence: 
                     cgaatatgcatGATATAGTTCCTCCTTTCAGCAAAAAACC"
     primer_bind     4111..4133
                     /label=2183
                     /note="color: black; sequence: 
                     gatcggtctcaCAGCctttcagcaaaaaacccctcaa"
     primer_bind     4112..4137
                     /label=3427
                     /note="color: black; sequence: 
                     GACCGACAATTGctttcagcaaaaaacccctcaagacc"
     primer_bind     4112..4137
                     /label=3426
                     /note="color: black; sequence: 
                     AGcagatctagactttcagcaaaaaacccctcaagacc"
     primer_bind     4112..4131
                     /label=2326
                     /note="color: black; sequence: 
                     atgtcgctggccgggtgacccggcggggacgaggcaagctctttcagcaaaaaacccct
                     c"
     primer_bind     4112..4128
                     /label=2831
                     /note="color: black; sequence: 
                     GTGGGCTCTATGGCTTCTGAGGCGGgtcgacTCTTTCAGCAAAAAACCC"
     primer_bind     4112..4127
                     /label=2914
                     /note="color: black; sequence: 
                     ATTTTCACGATATGTAAATAGTGAAATAGGCAAAAACgtcgacTCTTTCAGCAAAAAAC
                     C"
     primer_bind     4112..4126
                     /label=2848
                     /note="color: black; sequence: 
                     TCATACACCGAAAAGAAAGTTTAAGGGATCAATGAGCTgtcgacTCTTTCAGCAAAAAA
                     C"
     primer_bind     4112..4126
                     /label=2847
                     /note="color: black; sequence: 
                     GGCTTTGTTATGATTACATCAAATGTGGACTTCATACAgtcgacTCTTTCAGCAAAAAA
                     C"
     primer_bind     4117..4131
                     /label=4485
                     /note="color: black; sequence: 
                     atgcattttataattattataaaaactttcatatattaagtCTCGAGCAAAAAACCCCT
                     C"
     primer_bind     4132..4151
                     /label=2079
                     /note="color: black; sequence: AAGACCCGTTTAGAGGCCCC"
     primer_bind     4143..4162
                     /label=928
                     /note="color: black; sequence: AGAGGCCCCAAGGGGTTATG"
     primer_bind     complement(4148..4166)
                     /label=2325
                     /note="color: black; sequence: 
                     tgcgttatcccctgattctgtggataaccgtagtcggtctcctagcataaccccttggg
                     g"
     primer_bind     complement(4149..4167)
                     /label=2718
                     /note="color: black; sequence: 
                     TTTAGGCTTTGTTATGATTACATCAAATGTGGACTTCATACActagcataaccccttgg
                     g"
     primer_bind     complement(4149..4166)
                     /label=2832
                     /note="color: black; sequence: 
                     CCTAGAGCCCCAGCTGGTTCTTTCCTAGCATAACCCCTTGGG"
     primer_bind     complement(4149..4166)
                     /label=2722
                     /note="color: black; sequence: 
                     AATTATATCAGTTATTACCCGGGAATCTCGGTCGTAATGATTctagcataaccccttgg
                     g"
     primer_bind     complement(4149..4166)
                     /label=2720
                     /note="color: black; sequence: 
                     TAAGTCATACACCGAAAAGAAAGTTTAAGGGATCAATGAGCTctagcataaccccttgg
                     g"
     primer_bind     complement(4150..4166)
                     /label=4629
                     /note="color: black; sequence: 
                     ccatgatatctgttagtttttttcctgaaagaagcttGCTAGCATAACCCCTTGG"
     primer_bind     4162..4178
                     /label=4234
                     /note="color: black; sequence: CAATTGGCTAGTTATTGCTCAGC"
     primer_bind     4163..4183
                     /label=1918
                     /note="color: black; sequence: CTAGTTATTGCTCAGCGGTGG"
     primer_bind     complement(4163..4183)
                     /label=1917
                     /note="color: black; sequence: CCACCGCTGAGCAATAACTAG"
     primer_bind     complement(4182..4195)
                     /label=6202
                     /note="color: black; sequence: gcgtgtgaagttagctgctgcc"
     primer_bind     complement(4204..4230)
                     /label=2436
                     /note="color: black; sequence: 
                     TGAtgagatccggctgctaacaaagcccgaaa"
     primer_bind     complement(4204..4230)
                     /label=2440
                     /note="color: black; sequence: 
                     AGCCACCTGATGAGATCCGGCTGCTAACAAAGCCCGAAA"
     primer_bind     complement(4204..4230)
                     /label=2435
                     /note="color: black; sequence: 
                     ggtggttctCACCACCACCACCACCACTGAtgagatccggctgctaacaaagcccgaaa
                     "
     primer_bind     complement(4206..4236)
                     /label=14
                     /note="color: black; sequence: 
                     CATGGGTACCGGATCCTCTAGAAGATCCGGCTGCTAACAAAGCCCGA"
     primer_bind     complement(4206..4230)
                     /label=2377
                     /note="color: black; sequence: 
                     TCGGTCCGTGATGAGATCCGGCTGCTAACAAAGCCCGA"
     primer_bind     complement(4206..4230)
                     /label=2347
                     /note="color: black; sequence: 
                     GACATTAAAACAATTCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCG
                     A"
     primer_bind     complement(4208..4229)
                     /label=1185
                     /note="color: black; sequence: 
                     aactcgagGATCCGGCTGCTAACAAAGCCC"
     primer_bind     complement(4209..4231)
                     /label=2345
                     /note="color: black; sequence: 
                     CTCAAATCAGTAGACATTAAAACAATTTAAGATCCGGCTGCTAACAAAGCC"
     primer_bind     complement(4210..4230)
                     /label=2063
                     /note="color: black; sequence: 
                     CATCATCATCATCATCACTAGTGAGATCCGGCTGCTAACAAAGC"
     primer_bind     complement(4211..4236)
                     /label=6
                     /note="color: black; sequence: 
                     CATGtctagaAGATCCGGCTGCTAACAAAG"
     primer_bind     complement(4211..4230)
                     /label=5474
                     /note="color: black; sequence: tgagatccggctgctaacaaag"
     primer_bind     complement(4213..4230)
                     /label=1493
                     /note="color: black; sequence: 
                     ggattatgcgTGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=963
                     /note="color: black; sequence: 
                     caagaaagttTGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=2204
                     /note="color: black; sequence: 
                     CACCACCACCACCACCACTGATGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=1949
                     /note="color: black; sequence: 
                     CACCACCACCACCACCACTGATGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=2121
                     /note="color: black; sequence: 
                     ACCCACCACCACCACCACCACTGATGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=946
                     /note="color: black; sequence: 
                     cggctcccatcaccatcaccatcacTGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=2484
                     /note="color: black; sequence: 
                     cggctcccatcaccatcaccatcactaaTGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=1495
                     /note="color: black; sequence: 
                     ggcggctcctatccgtatgatgtgccggattatgcgTGAGATCCGGCTGCTAACAA"
     primer_bind     complement(4213..4230)
                     /label=1395
                     /note="color: black; sequence: 
                     ggtggcggctcctatccgtatgatgtgccggattatgcgTGAGATCCGGCTGCTAACAA
                     "
     primer_bind     complement(4215..4260)
                     /label=AW_BLA_from12_R
                     /note="color: black; sequence: 
                     cctttgatcttttctacggggtcttctagaagatccggctgctaac"
     primer_bind     4215..4260
                     /label=AW_BB141_F
                     /note="color: black; sequence: 
                     gttagcagccggatcttctagaagaccccgtagaaaagatcaaagg"
     primer_bind     complement(4215..4230)
                     /label=1314
                     /note="color: black; sequence: 
                     ctacaaataaTGAGATCCGGCTGCTAAC"
     primer_bind     complement(4215..4230)
                     /label=1092
                     /note="color: black; sequence: 
                     ctacaaagacgatgacgacaagTGAGATCCGGCTGCTAAC"
     primer_bind     complement(4215..4230)
                     /label=3208
                     /note="color: black; sequence: 
                     accggttctggtggttctggtCACCACCACCACCACCACTGAtgagatccggctgctaa
                     c"
     primer_bind     complement(4217..4238)
                     /label=1895
                     /note="color: black; sequence: 
                     taatcttccaataatctcacttttagtacttctagaagatccggctgcta"
     primer_bind     complement(4217..4238)
                     /label=1910
                     /note="color: black; sequence: 
                     ggagacgtactaatcttccaataatctcacttttagtacttctagaagatccggctgct
                     a"
     primer_bind     complement(4219..4238)
                     /label=218
                     /note="color: black; sequence: ACTTCTAGAAGATCCGGCTGC"
     primer_bind     complement(4219..4238)
                     /label=6539
                     /note="color: black; sequence: 
                     cgcgtttttaaattaataatgtaattcatatattatagtacttctagaagatccggctg
                     c"
     primer_bind     complement(4219..4238)
                     /label=359
                     /note="color: black; sequence: 
                     aaagatcctcaaggcaatagaataacagattttgatagtACTTCTAGAAGATCCGGCTG
                     C"
     primer_bind     complement(4219..4230)
                     /label=3666
                     /note="color: black; sequence: 
                     ggtggcggttctggtggcggttcaCACCACCACCACCACCACTGAtgagatccggctgc
                     "
     primer_bind     complement(4220..4238)
                     /label=7310
                     /note="color: black; sequence: 
                     agtgacccaagcgacgaggttatctctaatAGTACTTCTAGAAGATCCGGCTG"
     primer_bind     complement(4222..4238)
                     /label=343
                     /note="color: black; sequence: ACTTCTAGAAGATCCGGC"
     primer_bind     complement(4222..4238)
                     /label=389
                     /note="color: black; sequence: AGTACTTCTAGAAGATCCGGC"
     primer_bind     complement(4222..4238)
                     /label=3509
                     /note="color: black; sequence: 
                     gaatcttttaaacatAGTACTTCTAGAAGATCCGGC"
     primer_bind     complement(4222..4238)
                     /label=2545
                     /note="color: black; sequence: 
                     CTAATTGTCCTAATTCGCTACCACAagtacttctagaagatccggc"
     primer_bind     complement(4223..4238)
                     /label=4625
                     /note="color: black; sequence: 
                     caatatccaataaagAGTACTTCTAGAAGATCCGG"
     primer_bind     complement(4224..4238)
                     /label=3929
                     /note="color: black; sequence: 
                     ttattaaattctaAGTACTTCTAGAAGATCCG"
     primer_bind     4244..4267
                     /label=1824
                     /note="color: black; sequence: 
                     TAAGCAACTAGTGTAGAAAAGATCAAAGGATCTTCT"
     rep_origin      join(4267..4638,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     4280..4303
                     /label=2031
                     /note="color: black; sequence: TTTCTGCGCGTAATCTGCTGCTTG"
     primer_bind     complement(4280..4299)
                     /label=1216
                     /note="color: black; sequence: CAGCAGATTACGCGCAGAAA"
     primer_bind     4281..4300
                     /label=1644
                     /note="color: black; sequence: TTCTGCGCGTAATCTGCTGC"
     primer_bind     4281..4297
                     /label=1629
                     /note="color: black; sequence: ttctgcgcgtaatctgc"
     primer_bind     complement(4317..4349)
                     /label=2430
                     /note="color: black; sequence: 
                     CGTCGTCTACGTTTTCTCCAAGAGAGGatccGGTGGTGGTGGTAGTGGTG"
     primer_bind     complement(4373..4392)
                     /label=1832
                     /note="color: black; sequence: CTGCTGAAGCCAGTTACCTT"
     primer_bind     4447..4466
                     /label=5484
                     /note="color: black; sequence: CAAGAACTCTGTAGCACCGC"
     primer_bind     complement(4461..4478)
                     /label=1264
                     /note="color: black; sequence: CGAGGTATGTAGGCGGTG"
     primer_bind     complement(4465..4484)
                     /label=5485
                     /note="color: black; sequence: GCAGAGCGAGGTATGTAGGC"
     primer_bind     complement(4474..4483)
                     /label=5327
                     /note="color: black; sequence: 
                     CTAGAGGTCGACGGgaattcaatccggccagagcgagg"
     primer_bind     join(4612..4638,1..7)
                     /label=2173
                     /note="color: black; sequence: 
                     CTTGGAGCGAACGACCTACACCGAACTGAGATAC"
     primer_bind     join(4616..4638,1..7)
                     /label=2130
                     /note="color: black; sequence: 
                     gagcgaacgacctacaccgaactgagatac"
     primer_bind     complement(join(4619..4638,1..5))
                     /label=1960
                     /note="color: black; sequence: ATCTCAGTTCGGTGTAGGTCGTTCG"
     primer_bind     join(4632..4638,1..20)
                     /label=3761
                     /note="color: black; sequence: CCGAACTGAGATACCTACAGCGTGAGC"
     primer_bind     complement(join(4632..4638,1..20))
                     /label=3759
                     /note="color: black; sequence: GCTCACGCTGTAGGTATCTCAGTTCGG"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      481 agcgggcagt gagcgcaacg caatagtact ataatatatg aattacatta ttaatttaaa
      541 aacgcgtgat gacctataca taggaagatc tatagaaaca aaaagattaa taactttcaa
      601 atatcagaaa aatatagaaa catgtgataa gctcatagac atataaagga tccatgagat
      661 tcccatctat cttcaccgct gctttgttcg ctgcttcttc tgctttggct gctccagcta
      721 acaccaccac cgaagacgaa accgctcaaa tcccagctga agctgttatc gactactctg
      781 acttggaagg tgacttcgac gctgctgctt tgccattgtc taactctacc aacaacggtt
      841 tgtcttctac caacaccacc atcgcttcta tcgctgctaa ggaagaaggt gttcaattgg
      901 acaagagaga agctgacgca caggtgcact cctcgctgca ggaaagcggc ggcggcctgg
      961 tgcaggcggg cggcagcctg cgcctgagct gcgcggcgag cggcaatatt tttgacgctg
     1021 acatcatggg ctggtatcgc caggcgccgg gcaaagaacg cgaacttgtt gccagtatta
     1081 ctgacggtgg tagtaccaat tatgcggata gcgtgaaagg ccgctttacc attagccgcg
     1141 ataacgcgaa aaacaccgtg tatctgcaga tgaacagcct gaaaccggaa gataccgcgg
     1201 tgtattattg cgcggctatc gcttacccgg acatcccgac ttacttcgac tacgactctg
     1261 actatttcta ttggggccag ggcacccagg tgaccgtgag aggaggcagc ggatccaagg
     1321 acaatagctc gacgattgaa ggtagatacc catacgacgt tccagactac gctctgcagg
     1381 ctagtggtgg tggtggttct ggtggtggtg gttctggtgg tggtggttct gctagcgacg
     1441 tccaggaact gacaactata tgcgagcaaa tcccctcacc aactttagaa tcgacgccgt
     1501 actctttgtc aacgactact attttggcca acgggaaggc aatgcaagga gtttttgaat
     1561 attacaaatc agtaacgttt gtcagtaatt gcggttctca cccctcaaca actagcaaag
     1621 gcagccccat aaacacacag tatgtttttt gaagaagact tgtcatgaaa aaaaaaaaaa
     1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1741 aaacctgtca ccggatgtgt tttccggtct gatgagtccg tgaggacgaa acagggaatt
     1801 cgcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     1861 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     1921 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctcgag ctccgtttct
     1981 attatgaatt tcatttataa agtttatgta caaatatcat aaaaaaagag aatctttttt
     2041 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
     2101 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
     2161 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
     2221 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
     2281 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
     2341 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
     2401 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     2461 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     2521 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     2581 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
     2641 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     2701 taacagacat tttataatta ttataaaaac tttcatataa gagatataaa atttaatatg
     2761 gaagaaataa gacaagaaag atacaaccaa atgaaagaag ctctaaatag tgttgaaggt
     2821 tataaaggaa aaattgtagc ctcagactca gattggtgtt tcaaagatcc tcaaggcaat
     2881 agaataacag attttgatag tactttaagc cagccccgac acccgccaac acccgctgac
     2941 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3001 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaaggca
     3061 aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt taccaatgct taatcagtga
     3121 agcccctatt tcagcaatct gtctatttcg ttcgtccata gttgcctgac tacccgtcgt
     3181 gtagataact acgatacggg aaggcttacc atctggtcca agtgcggcaa tgataccccg
     3241 tgaaccacgc tctcctgccc cagatttatc agcaataaac cagccagcag gaagtgccga
     3301 acgcagtagt ggtcctgcaa ctttatccgc ttcaagccag tctattaatt gttgccgtga
     3361 agccagagta agtagttcgc cagttaatag tttgcgtaat gttgttgcca ttgccgcagg
     3421 cattgtggtg tctcgctcgt cgtttggtat ggcttcattc agttctggtt cccaacggtc
     3481 aaggcgagtt acatggtctc ccatattgtg caaaaaagcg gttaattcct tcggtccacc
     3541 gatggttgtc agaagtaagt tcgccgcagt gttatcactc atcgttatgg cggcactaca
     3601 taattctctt accgtcattc cgtccgtaag gtgcttttct gtcactgggg agtattcaac
     3661 caagtcattc tgcgaatagt gtattcgtcg tccgagttgc tcttgccccg catctacacg
     3721 ggataatact gctccacata gcagaacttt gaaagtgctc atcattggaa aacgctcttc
     3781 agggcgaaaa ctctcaagga ttttaccgct attcaagtcc agttctatgt atcccactcg
     3841 tgcccccaat tggtcctctg cgtcttttac tttcacgagc gtttctgggt gagcaaaaac
     3901 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctga tgagcggata
     4021 catatttgaa tgtatttaga aaaataaaca aataggggaa ttaaaaaaaa gcccgctcat
     4081 taggcgggct actagtcgga tatagttcct cctttcagca aaaaacccct caagacccgt
     4141 ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
     4201 tcctttcggg ctttgttagc agccggatct tctagaagac cccgtagaaa agatcaaagg
     4261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4381 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4621 aacgacctac accgaact
//
