LOCUS       i-150_pUASTattB-       10341 bp    DNA     circular SYN 13-JAN-2017
DEFINITION  Inverted 150_pUASTattB-GFPMsp300KASH_WIDESEQ072315_VMW
ACCESSION   i-150_pUASTattB-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    1079..1663
                     /gene="P element 5' end"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             complement(2656..3516)
                     /gene="ampR: class A beta-lactamase"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3671..3956
                     /gene="attB"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4744..4981
                     /gene="P element 3' end"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5025..5141
                     /gene="5XUAS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5148..5375
                     /gene="hsp70 minimal promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             5428..6159
                     /gene="EGFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             5428..6336
                     /gene="EGFP-Msp300KASH"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             6172..6336
                     /gene="Msp300-KASH"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    6343..7042
                     /gene="SV40 polyA region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    join(7059..10341,1..1066)
                     /gene="white eye marker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7059..7537
                     /gene="white promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             join(7538..10341,1..298)
                     /gene="white ORF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7538..7609
                     /gene="white exon 1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg
       61 gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga
      121 aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac
      181 gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt
      241 gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc
      301 gacatatatc cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac
      361 tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat
      421 gacctataga atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt
      481 cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac
      541 ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata
      601 attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc
      661 gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt
      721 cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata
      781 tttgtatgta caatttattt gctccaatat atttgtatat atttccctca cagctatatt
      841 tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt
      901 agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc
      961 aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat
     1021 aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg
     1081 ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt
     1141 ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt
     1201 tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct
     1261 tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt
     1321 taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac
     1381 aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa
     1441 tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc
     1501 aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg
     1561 cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa
     1621 gcttcggcta tcgacgggac caccttatgt tatttcatca tgggccagac ccacgtagtc
     1681 cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc ccgaactggg
     1741 gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg cccattggtt
     1801 aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc atttgaggta
     1861 tactggcacc gagcccgagt tcaagaagaa ggcgtttttc cataggctcc gcccccctga
     1921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcaattcggc
     3661 ttcaggtacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg
     3721 tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg
     3781 ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct
     3841 cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg
     3901 cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacaagcc
     3961 gaattgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4021 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4081 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4141 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
     4261 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     4321 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     4381 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     4441 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     4501 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     4561 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     4621 aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
     4681 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
     4741 accatgatga aataacataa ggtggtcccg tcggcaagag acatccactt aacgtatgct
     4801 tgcaataagt gcgagtgaaa ggaatagtat tctgagtgtc gtattgagtc tgagtgagac
     4861 agcgatatga ttgttgatta acccttagca tgtccgtggg gtttgaatta actcataata
     4921 ttaattagac gaaattattt ttaaagtttt atttttaata atttgcgagt acgcaaagct
     4981 tggctgcatc caacgcgttg ggagctctcc ggatccaagc ttgcatgcct gcaggtcgga
     5041 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     5101 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     5161 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     5221 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     5281 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     5341 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     5401 ggaattcgtt aacagatctg cggccgcatg agtaaaggag gagaactttt cactggagtt
     5461 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     5521 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     5581 aaactacctg ttccatggcc aaccctggtc accaccctga cctacggcgt gcagtgcttc
     5641 tcccgttacc ctgatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga
     5701 tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa
     5761 gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag
     5821 gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac
     5881 atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc
     5941 gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc
     6001 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     6061 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     6121 ggcatggatg aactatacaa agctgctgct gctgctgctg ggcgcgccat gcgcgttgca
     6181 cgtgcctcgc tgcccattca ggcgctcatg ctgctgcttt taggtgtggc caccctggtg
     6241 ccccacggtg aggactacac ctgtatgttc tccaacacct ttgcgcgcag cctggagccc
     6301 atgttgtcgt atccccacgg accgccaccc acgtaatcta gaggatcttt gtgaaggaac
     6361 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     6421 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     6481 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     6541 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     6601 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     6661 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     6721 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     6781 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     6841 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6901 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7021 gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg taagttaata
     7081 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     7141 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     7201 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     7261 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     7321 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     7381 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     7441 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     7501 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     7561 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     7621 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     7681 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     7741 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     7801 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     7861 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     7921 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     7981 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     8041 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     8101 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     8161 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     8221 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     8281 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     8341 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     8401 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     8461 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     8521 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     8581 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     8641 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     8701 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     8761 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     8821 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     8881 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     8941 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     9001 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     9061 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     9121 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     9181 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     9241 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     9301 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     9361 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     9421 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     9481 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     9541 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     9601 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     9661 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     9721 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     9781 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     9841 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     9901 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     9961 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
    10021 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
    10081 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
    10141 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
    10201 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
    10261 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
    10321 ctggactacg tgggtctggc c
//
