LOCUS       Exported               12071 bp ds-DNA     circular SYN 31-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Yetkin
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    114..138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     154..172
                     /label=YI102
     primer_bind     180..217
                     /label=YI011
     promoter        200..1292
                     /label=FRO6 pro
     primer_bind     complement(1270..1318)
                     /label=YI012
     CDS             join(1293..1307,1498..3315)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          1308..1497
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     primer_bind     complement(1515..1534)
                     /label=YI202
     CDS             3322..3339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3352..4058
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(4298..5014)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5650..5674
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6202..6993
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7237..7825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8011..8151
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8495..8689
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8755..9822)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10256..10885)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     11927..11946
                     /label=YI207
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctaagctc gatgctctca aggccaaaga acattatgga aattcgaaga
      241 tatattatcg atgcatataa tttatataat cacagtctgt atgtattatg gaaacaactt
      301 taactaattt ttgagattga tgctacttca tgatagcaat ttcttagttc ttttgagtta
      361 ataacttaat atataaatat aatggtacaa caaccattac aatcgtcatt ataatcatct
      421 tatccatact caatagtcaa tactctcacc ttccctaaag tgtggttgaa atcaaacttt
      481 actctctcta ggcctgatta taattctttt ttgtctacac atacaacttc atgagactta
      541 attataattt cttagtgttc ttttgttgac atgtaaatcg tagctaagtc taagtggtac
      601 ttttatttgt tattggattt tgtttcggtt acttaaaagt gtaatgaaga atttttttga
      661 ctatatacat ctaagttttt ccttggactg attataactt gtgtgttcga gattgataca
      721 acttcatgat agtcgattat tcttgtttaa cgtaacacat tgttagatag tgtcatttac
      781 cagaaacaat ctaacagtga aactttgcaa atttatctct ctgctggatc ttgaacgcgg
      841 aaaccgaata atgtttcttt cgagctaatc cgaatgatgt tgaaattcta tatgttcaga
      901 ttcaggtaat gtaagcttat ccaataacca tcatagtcgt cataataacc atcttatcta
      961 tactaaagta gtaagtactc tcagtctctc accttaccct taattgtggt tgaaatcaac
     1021 ctaacgtgac actctctccc aagtacgtta ctctcacgtg ttaatgacac tctcattttt
     1081 ttttatagtt ttttttattg tgtactagga ttgttgtccg aaggaatctt tcataaatga
     1141 gaatgataga aacctcaatg gtccagaaaa ttttacttga ctcttctaag aatatcttat
     1201 tagtttatct ctagtaacgc caccttaaaa gccgatgctg cttctgagcc aacttctcat
     1261 cacaacactg agaagtggaa attcaaataa agatggtaga tctgagggta aatttctagt
     1321 ttttctcctt cattttcttg gttaggaccc ttttctcttt ttattttttt gagctttgat
     1381 ctttctttaa actgatctat tttttaattg attggttatg gtgtaaatat tacatagctt
     1441 taactgataa tctgattact ttatttcgtg tgtctatgat gatgatgata gttacagaac
     1501 cgacgaacta gtctgtaccc gatcaacacc gagacccgtg gcgtcttcga cctcaatggc
     1561 gtctggaact tcaagctgga ctacgggaaa ggactggaag agaagtggta cgaaagcaag
     1621 ctgaccgaca ctattagtat ggccgtccca agcagttaca atgacattgg cgtgaccaag
     1681 gaaatccgca accatatcgg atatgtctgg tacgaacgtg agttcacggt gccggcctat
     1741 ctgaaggatc agcgtatcgt gctccgcttc ggctctgcaa ctcacaaagc aattgtctat
     1801 gtcaatggtg agctggtcgt ggagcacaag ggcggattcc tgccattcga agcggaaatc
     1861 aacaactcgc tgcgtgatgg catgaatcgc gtcaccgtcg ccgtggacaa catcctcgac
     1921 gatagcaccc tcccggtggg gctgtacagc gagcgccacg aagagggcct cggaaaagtc
     1981 attcgtaaca agccgaactt cgacttcttc aactatgcag gcctgcaccg tccggtgaaa
     2041 atctacacga ccccgtttac gtacgtcgag gacatctcgg ttgtgaccga cttcaatggc
     2101 ccaaccggga ctgtgaccta tacggtggac tttcaaggca aagccgagac cgtgaaagtg
     2161 tcggtcgtgg atgaggaagg caaagtggtc gcaagcaccg agggcctgag cggtaacgtg
     2221 gagattccga atgtcatcct ctgggaacca ctgaacacgt atctctacca gatcaaagtg
     2281 gaactggtga acgacggact gaccatcgat gtctatgaag agccgttcgg cgtgcggacc
     2341 gtggaagtca acgacggcaa gttcctcatc aacaacaaac cgttctactt caagggcttt
     2401 ggcaaacatg aggacactcc tatcaacggc cgtggcttta acgaagcgag caatgtgatg
     2461 gatttcaata tcctcaaatg gatcggcgcc aacagcttcc ggaccgcaca ctatccgtac
     2521 tctgaagagt tgatgcgtct tgcggatcgc gagggtctgg tcgtgatcga cgagactccg
     2581 gcagttggcg tgcacctcaa cttcatggcc accacgggac tcggcgaagg cagcgagcgc
     2641 gtcagtacct gggagaagat tcggacgttt gagcaccatc aagacgttct ccgtgaactg
     2701 gtgtctcgtg acaagaacca tccaagcgtc gtgatgtgga gcatcgccaa cgaggcggcg
     2761 actgaggaag agggcgcgta cgagtacttc aagccgttgg tggagctgac caaggaactc
     2821 gacccacaga agcgtccggt cacgatcgtg ctgtttgtga tggctacccc ggagacggac
     2881 aaagtcgccg aactgattga cgtcatcgcg ctcaatcgct ataacggatg gtacttcgat
     2941 ggcggtgatc tcgaagcggc caaagtccat ctccgccagg aatttcacgc gtggaacaag
     3001 cgttgcccag gaaagccgat catgatcact gagtacggcg cagacaccgt tgcgggcttt
     3061 cacgacattg atccagtgat gttcaccgag gaatatcaag tcgagtacta ccaggcgaac
     3121 cacgtcgtgt tcgatgagtt tgagaacttc gtgggtgagc aagcgtggaa cttcgcggac
     3181 ttcgcgacct ctcagggcgt gatgcgcgtc caaggaaaca agaagggcgt gttcactcgt
     3241 gaccgcaagc cgaagctcgc cgcgcacgtc tttcgcgagc gctggaccaa cattccagat
     3301 ttcggctaca agaacgctag ccatcaccat caccatcacg tgtgaaggat cctgctttaa
     3361 tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
     3421 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
     3481 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
     3541 tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg
     3601 tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt tattattaca
     3661 aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc
     3721 ttattcaaat ttcaaaaggc cccaggggct agtatctacg acacaccgag cggcgaacta
     3781 ataacgttca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
     3841 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
     3901 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
     3961 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
     4021 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctctagagc gccactggat
     4081 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     4141 taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct aattccctta
     4201 tctgggaact actcacacat tattatagag agagatagat ttgtagagag agactggtga
     4261 tttcagcgat gcagccgggc ggcgcttaca gctcgtcctt gtacagctcg tccatgccga
     4321 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     4381 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     4441 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     4501 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
     4561 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     4621 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     4681 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     4741 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     4801 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     4861 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     4921 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     4981 ccccggtgaa cagctcctcg cccttgctca ccatgtcggc cgaggataat gataggagaa
     5041 gtgaaaagat gaaaaagaga aaaagattag tcttcaccat ggctatcgtt cgtaaatggt
     5101 gaaaattttc agaaaatagc ttttgcttta aaagaaatga tttaaattgc tgcaatagaa
     5161 gtagaatgct tgattgcttg agattcgttt gttttgtata tgttgtgttg agaattcgcc
     5221 cttgttttgc tatttgtgta tgttttcttg ttttggtttg agtgaagagt gtagtgaagg
     5281 gttggtttgt atttataggt gagaaaggag atttgcatgg caatcacgtg taagaatgca
     5341 tgcatgcatg agtgtgtgtg gagagagaga gatgctctta cgttgttgtc gggattatgt
     5401 gttggaaaag agtgacaagt gacgaacaaa acaataccac tcatcatagc tccgcttatg
     5461 catgctatgt gtgatttgta gctcttgttc tgacatgaca ctatgaacaa ttttttaaaa
     5521 agaaggttaa acttttattt aaataaaaac cctatgttaa tttggtttca agggcgaatt
     5581 caattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     5641 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     5701 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     5761 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     5821 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     5881 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     5941 cctcgttcgg ttcgtaagct gtaatgcaag tagcgtaact gccgtcacgc aactggtcca
     6001 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttcttgtt
     6061 atgacatgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     6121 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     6181 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     6241 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     6301 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     6361 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     6421 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     6481 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     6541 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     6601 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     6661 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     6721 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     6781 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     6841 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     6901 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     6961 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     7021 acgccgcttc gccggcgtta actcaagcga ttagatgcac taagcacata attgctcaca
     7081 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     7141 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7981 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8041 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     8101 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8281 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     8341 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     8401 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     8461 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     8521 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     8581 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     8641 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8701 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8761 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8821 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8881 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8941 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     9001 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     9061 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     9121 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     9181 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     9241 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     9301 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     9361 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     9421 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     9481 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     9541 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     9601 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9661 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9721 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9781 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc gattcctcgg
     9841 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc
     9901 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
     9961 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
    10021 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
    10081 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
    10141 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
    10201 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
    10261 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
    10321 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
    10381 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
    10441 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
    10501 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
    10561 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    10621 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    10681 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    10741 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    10801 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    10861 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    10921 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    10981 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    11041 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    11101 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    11161 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    11221 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    11281 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    11341 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    11401 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    11461 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    11521 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    11581 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    11641 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    11701 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    11761 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    11821 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    11881 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    11941 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    12001 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    12061 atcaaagtac t
//
