LOCUS       Exported               16496 bp ds-DNA     circular SYN 05-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pYI012(GH3.17)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16496)
  AUTHORS   Yetkin
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    84..85
                     /label=Insertion GT-GaT
                     /note="In original vector"
     misc_feature    114..138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     118..138
                     /label=YI102
     primer_bind     180..221
                     /label=YI020
     promoter        200..5572
                     /label=GH3.17 pro
     misc_feature    279..281
                     /label=deletion 3 bp
     primer_bind     complement(333..353)
                     /label=YI114
     primer_bind     970..989
                     /label=YI213
     primer_bind     1539..1558
                     /label=YI212
     misc_feature    1700..1701
                     /label=deletion 2 bp
     primer_bind     2914..2936
                     /label=YI210
     primer_bind     complement(3241..3263)
                     /label=YI211
     primer_bind     4506..4528
                     /label=YI208
     primer_bind     complement(4772..4793)
                     /label=YI209
     5'UTR           5573..5717
     primer_bind     complement(5694..5743)
                     /label=YI024
     CDS             join(5718..5732,5923..7740)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          5733..5922
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     primer_bind     complement(5940..5959)
                     /label=YI202
     CDS             7747..7764
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7777..8483
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8723..9439)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    10075..10099
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10627..11418
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11662..12250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    12436..12576
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      12920..13114
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13180..14247)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(14681..15310)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     16352..16371
                     /label=YI207
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctaagctc ataaacattt acctttcatg gggattacat ttttaagtct
      241 atagctatat ccattattca gttatgtgca taaatcagct tcttcttctt cttcttttgt
      301 aactaaagca tgcataataa gaaagccgat attggacata gaccaacaaa gctatgtaat
      361 tggaatgatc agccaattag gtaacacatc aaagaaaaga aataatagat cctccgatgt
      421 ttggtaaatt tattaataat tatttacaca agttggtaat tgtggaataa tttgtctaca
      481 caaggttgac aattgtggct gagcgattat gttagagtgc atcaagttga aagtgtagga
      541 ttttgttgga ctcttttggt gccttggtaa aataagatga tataatcggg gtggttttga
      601 ttgagcttcc accaagatag agtgttcata ttttattagc tagtagactt aaagatttat
      661 gtcactcatc gtaatgagaa atttctacaa tttccactta tatacaatgc aaaacaaact
      721 aaattggtac aacaaacata tattcatgtg aacctcatat gttcctcatg catagttacc
      781 aatagttcgt atctctcgtc ttttattggg agttatctta tcaatgtgct gaatttggct
      841 ataatgatgt ttttcaaatt gagcacacga ccatgagtac tttatttttt gttttttcaa
      901 aatgacttaa aatcgttgtg tatcatttat cgagtctcta ttcgaaaggc cagactaact
      961 cgatttggac tggtgccaag acaaaagctt gtttttgggg aggtgcggaa aggtgaaacc
     1021 ctccgtagta cgtgatgtat ctcaggaata gaaatgttct gattttatca tttaatcttt
     1081 gtggagtttg atgaatatat aaaggtgatt tagagttttt atgtcattat ttacatgatt
     1141 actaaaagaa accctaggct aaaagatatt tgcctcaaaa ttgaaaaagt tgaatatttt
     1201 aacactttac taatatatac tttgttaagc accatttttc cctcctattt tctctcctta
     1261 tttcaagact acatgtgccc tcatagagac acttgctttt gcatttcact ttatgttaat
     1321 ctgagcaata aaagacctct aaagaccata tctaagcaaa tccctgaaca aatgtcctaa
     1381 tcaacaattt aattaatact agtttctttt cggtttcaag gcgtttacca aaaaccaaac
     1441 aactctttac acaaaactag ttctattcac tttgggatcg gtgatgtgct tattgctatt
     1501 aattaatact agatatgatc atagtctctt ctattccaag caacgcatac cactttgttt
     1561 atgtggtaca ccagtcattt acttgcctac tcaatagaag ttttcacttt tgattttgta
     1621 tttctctcca taagtataat attaggaata attaaagcta tataagtaaa gaatgcgaaa
     1681 atcttgatgg atttaacatt atatatatat atatatatat atatctttct agatttagat
     1741 ttaaactcgg attgttatgc aatgacgcaa ctagtataaa tgactaggct acatcttaca
     1801 tgttatggtc agctaaaaat tgttatgtta aatgtgtgat taaaattaat ttaattgagg
     1861 tgtatgtaca cacgaaggat tttatattgc tatagataga taaataaata aaaataattt
     1921 atactgtttg gactgaccaa gttataacac taagaaaaac aataatatgg gaattccaca
     1981 gtttattttt cactaatttt caaaatccgt tatttttttt taaaaaatgt tctaactaaa
     2041 cgagagaatt aaagttatat tctcttcaag gaagctggca aatttattta aagtctcact
     2101 tgtacatcaa acgtacgttg aatcatttat aagagctgag tgtgtaagat acgtaattta
     2161 aatttgacaa gtcattagct ttgccaaata tttcgaacac tatgaagtct gtgatcatta
     2221 acttattttt cttgtcaaca gaagtcacat gttacatcac aaatcctaat tagtagaaca
     2281 ctagaaatca gatttagcta taaccatcca acatataagc tatacacttg atttttggag
     2341 acttgtgggt aaactaacta ctcttgaaat gtgagactca catgtaagtc ttgtttcggt
     2401 aattaatctc taaattttaa tcttatgtaa ccctacgtta atagaccgaa taatattttt
     2461 taacttgagt aaatcgaaat caatactcat attactagat tataatctca tatcatattc
     2521 ctaattttta ttgtattttt tctgtttctc ttattgccat ttatctgaaa ttttcttttt
     2581 aatttatttt ctcttatggt tttgtcgtga ttcaggagta gtaattaatt cacgtcatgc
     2641 gcgatttaat atgaatttaa aaatgttatc tcagtccatg agaagtagta attgtgttgt
     2701 gcgtattggt ttgaagtagt ccaccaaaag agcaataaat gcaaagctcg taactttgac
     2761 ctattttttg tttgtcttat tacactactg attttttttt cctttgccat gtctatacag
     2821 tatacactat tattctctct tatttttcaa tatatgaact acataacaca tttaatataa
     2881 acaacatgca ttagtttaca ttagatagaa aacttaagct gttacatgat ccaggacttt
     2941 ggtatcctag tggcatgtcc gcatgtgttg caatctctta aaactctcaa acagcaacta
     3001 caacatattc tacatagtct ctagatttgt ttgggtcaac catatagtct cgaatttaaa
     3061 cgtcaaattc acagtacgta tatggttctt aaccactgta tgccaattat tatcactaac
     3121 cctttcaatt atactgtatt tgaaacccga atcttatatt aaccttagcg ttttaggctt
     3181 aagtttattg ttgactttcg aactgttaac atacttttat gggtgtatat atatgactta
     3241 tgctacaacc aacttttgat cctttaactt ctagcataag tccatgactg tagtttccaa
     3301 tggatatgct tagaatcccg ttgacatata ttttgagtga agaattagat gatgaacaat
     3361 ataaaagtat ttaagccttt taacaaaaat ataaaaatat attaaaaaaa aatcctgtta
     3421 ataaattctt ttacgggaat gaccaatcaa aatatatgtg ggcttattgt tccacgaacc
     3481 aggatgaaac acatcagatg atacatcatt caaaggtttt gcgaaagttc tttttatcta
     3541 actaggattt cggatggttt gcctaaaata acataataga agaaaatatg ggcgttacgt
     3601 atcaggaaaa tattatcaca aaacaacatt tcaagaattc attactttta aaacagaaat
     3661 agtaaggtta gaaaatattc aaaaagagtg catgtctttt aaagttgatg ggtcggtctt
     3721 atagattgcg catgtcctgt atctctccca aacattgtgg accggtctat gtatttgtga
     3781 aaggaaatat gccaactttt actagtctct ctctcgcatg tgtgtgagtg aaatagtttt
     3841 aatatttcaa atttatttcc aaaacaattc ttacaaggat tatcaactgc gcagcttaaa
     3901 aaatccaatt atactcaact agtattatca ctctatagtt cagaatactt ttcttgaaaa
     3961 tatgaaatat atataaacta taaattttat gtttttatat tatttaatga agacaatcaa
     4021 tgggattaag accattaacg tgtgaaacat aagacatata tgatttatcg taaggttgag
     4081 aatctatgta tgctttttca gggaccaaaa ggaatatata acatcccttc agaaaaaaaa
     4141 agatttacat gatttgggtt gttacgtttc aaaaagaaaa ttaaaactct agtttaaaat
     4201 ctggtagatg tttcagcccc ttgtttaata aatctgaaac gagattctca ccatcatttt
     4261 tttgggataa attatcacta ttattttcat atatatgtta ttttaaattg attattaatt
     4321 atttagtaga atggtataat catgaattat aaacaaatac gtggaataaa ataactaaat
     4381 cgagatgttt aattaaatca acttaattgt caaggaaaag atttatttta gttttatcat
     4441 taaatgacta aaccaaaata aactaagcaa tataaaaagc ctaaagcaaa ggaattgacg
     4501 aaacatgcct aaagtgtatg acatagcgta tttgaatgaa atttgttcac gagaaaaaga
     4561 tcatcttctc caaaccctct caggcctctt ttctctctgt tcacatctgt ttcctcgtca
     4621 cacaagacat atacattaac ctaattcaac tgttccatgt cttttaaagt tttcttgatg
     4681 gaattaagct gatgcatgtt attttatgtt gtaattatca tcatttatgg ttagttagtc
     4741 aattggtcta tttatattat atgtatatca acgtaacgac cctatcatat gcatttggat
     4801 ctgcatcacc aagatgatga ctttataata tccaagagat ttaacaatct taacaatcct
     4861 tctctctctt aatcttgatt gccataagtc aaacttgaga ttatttaatt actatatgga
     4921 gcccaattat cgcatgtaaa tgaggttaat ataaataggc agatttaaac taacatatat
     4981 gtcaatggaa tttgaataac taagagttgt ttagtatttc acagcaatgc aaatatacag
     5041 gttttaagaa caatatgcaa atgaatttaa gggatatata aaaagttctt aaagaaaatt
     5101 ccatagccac atatataaag aatttgttaa cttaagctat catatacttc tttttagtat
     5161 aaactttatt tgaaaaattt gcatgtgagt tcataaaggt ttcaacgagt tcctaaataa
     5221 aatctagcta agaggcaata ttagatgagt atagtgaaga ccaagtgagt atatcttttg
     5281 gcagacatat gcaaatcttg tgaatatgat tggattgata ttattcaacg gatcataaag
     5341 aaagagcttg attggaggat taaacagcat ataaacaaat atgatattaa acaaaaaata
     5401 acatataaaa ttatagggaa agcctgcaca ggtaatattg accaacaata gcttctttct
     5461 aactagacaa ggccatgtgt gttgtaggac ctcccaaata tatattttct aataattatt
     5521 tcatcatttg ccaatttatt atataaccat ttctcatcct tctcctcata ccaccaaaaa
     5581 ccaaccttca agaacagaga agaagtgatt ctctaaacaa caaaaataaa ataaaactct
     5641 tctttctcta tttctgttga aggtacctca tcattgcaac acaaacttaa agctttgttt
     5701 gtgtctgctt tcagaaaatg gtagatctga gggtaaattt ctagtttttc tccttcattt
     5761 tcttggttag gacccttttc tctttttatt tttttgagct ttgatctttc tttaaactga
     5821 tctatttttt aattgattgg ttatggtgta aatattacat agctttaact gataatctga
     5881 ttactttatt tcgtgtgtct atgatgatga tgatagttac agaaccgacg aactagtctg
     5941 tacccgatca acaccgagac ccgtggcgtc ttcgacctca atggcgtctg gaacttcaag
     6001 ctggactacg ggaaaggact ggaagagaag tggtacgaaa gcaagctgac cgacactatt
     6061 agtatggccg tcccaagcag ttacaatgac attggcgtga ccaaggaaat ccgcaaccat
     6121 atcggatatg tctggtacga acgtgagttc acggtgccgg cctatctgaa ggatcagcgt
     6181 atcgtgctcc gcttcggctc tgcaactcac aaagcaattg tctatgtcaa tggtgagctg
     6241 gtcgtggagc acaagggcgg attcctgcca ttcgaagcgg aaatcaacaa ctcgctgcgt
     6301 gatggcatga atcgcgtcac cgtcgccgtg gacaacatcc tcgacgatag caccctcccg
     6361 gtggggctgt acagcgagcg ccacgaagag ggcctcggaa aagtcattcg taacaagccg
     6421 aacttcgact tcttcaacta tgcaggcctg caccgtccgg tgaaaatcta cacgaccccg
     6481 tttacgtacg tcgaggacat ctcggttgtg accgacttca atggcccaac cgggactgtg
     6541 acctatacgg tggactttca aggcaaagcc gagaccgtga aagtgtcggt cgtggatgag
     6601 gaaggcaaag tggtcgcaag caccgagggc ctgagcggta acgtggagat tccgaatgtc
     6661 atcctctggg aaccactgaa cacgtatctc taccagatca aagtggaact ggtgaacgac
     6721 ggactgacca tcgatgtcta tgaagagccg ttcggcgtgc ggaccgtgga agtcaacgac
     6781 ggcaagttcc tcatcaacaa caaaccgttc tacttcaagg gctttggcaa acatgaggac
     6841 actcctatca acggccgtgg ctttaacgaa gcgagcaatg tgatggattt caatatcctc
     6901 aaatggatcg gcgccaacag cttccggacc gcacactatc cgtactctga agagttgatg
     6961 cgtcttgcgg atcgcgaggg tctggtcgtg atcgacgaga ctccggcagt tggcgtgcac
     7021 ctcaacttca tggccaccac gggactcggc gaaggcagcg agcgcgtcag tacctgggag
     7081 aagattcgga cgtttgagca ccatcaagac gttctccgtg aactggtgtc tcgtgacaag
     7141 aaccatccaa gcgtcgtgat gtggagcatc gccaacgagg cggcgactga ggaagagggc
     7201 gcgtacgagt acttcaagcc gttggtggag ctgaccaagg aactcgaccc acagaagcgt
     7261 ccggtcacga tcgtgctgtt tgtgatggct accccggaga cggacaaagt cgccgaactg
     7321 attgacgtca tcgcgctcaa tcgctataac ggatggtact tcgatggcgg tgatctcgaa
     7381 gcggccaaag tccatctccg ccaggaattt cacgcgtgga acaagcgttg cccaggaaag
     7441 ccgatcatga tcactgagta cggcgcagac accgttgcgg gctttcacga cattgatcca
     7501 gtgatgttca ccgaggaata tcaagtcgag tactaccagg cgaaccacgt cgtgttcgat
     7561 gagtttgaga acttcgtggg tgagcaagcg tggaacttcg cggacttcgc gacctctcag
     7621 ggcgtgatgc gcgtccaagg aaacaagaag ggcgtgttca ctcgtgaccg caagccgaag
     7681 ctcgccgcgc acgtctttcg cgagcgctgg accaacattc cagatttcgg ctacaagaac
     7741 gctagccatc accatcacca tcacgtgtga aggatcctgc tttaatgaga tatgcgagac
     7801 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
     7861 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     7921 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
     7981 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
     8041 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
     8101 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
     8161 aaggccccag gggctagtat ctacgacaca ccgagcggcg aactaataac gttcactgaa
     8221 gggaactccg gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc
     8281 gtccgctcta ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa
     8341 ccgacttgct gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa
     8401 gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc
     8461 gacaacatgt cgaggctcag caggacctct agagcgccac tggattttgg ttttaggaat
     8521 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     8581 tgctcaacac atgagcgaaa ccctataaga accctaattc ccttatctgg gaactactca
     8641 cacattatta tagagagaga tagatttgta gagagagact ggtgatttca gcgatgcagc
     8701 cgggcggcgc ttacagctcg tccttgtaca gctcgtccat gccgagagtg atcccggcgg
     8761 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     8821 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     8881 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     8941 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     9001 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     9061 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     9121 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     9181 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     9241 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     9301 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     9361 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     9421 cctcgccctt gctcaccatg tcggccgagg ataatgatag gagaagtgaa aagatgaaaa
     9481 agagaaaaag attagtcttc accatggcta tcgttcgtaa atggtgaaaa ttttcagaaa
     9541 atagcttttg ctttaaaaga aatgatttaa attgctgcaa tagaagtaga atgcttgatt
     9601 gcttgagatt cgtttgtttt gtatatgttg tgttgagaat tcgcccttgt tttgctattt
     9661 gtgtatgttt tcttgttttg gtttgagtga agagtgtagt gaagggttgg tttgtattta
     9721 taggtgagaa aggagatttg catggcaatc acgtgtaaga atgcatgcat gcatgagtgt
     9781 gtgtggagag agagagatgc tcttacgttg ttgtcgggat tatgtgttgg aaaagagtga
     9841 caagtgacga acaaaacaat accactcatc atagctccgc ttatgcatgc tatgtgtgat
     9901 ttgtagctct tgttctgaca tgacactatg aacaattttt taaaaagaag gttaaacttt
     9961 tatttaaata aaaaccctat gttaatttgg tttcaagggc gaattcaatt cggcgttaat
    10021 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
    10081 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
    10141 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
    10201 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
    10261 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
    10321 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctcg ttcggttcgt
    10381 aagctgtaat gcaagtagcg taactgccgt cacgcaactg gtccagaacc ttgaccgaac
    10441 gcagcggtgg taacggcgca gtggcggttt tcatggcttc ttgttatgac atgttttttt
    10501 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
    10561 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
    10621 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
    10681 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
    10741 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
    10801 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
    10861 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
    10921 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
    10981 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
    11041 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
    11101 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
    11161 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
    11221 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
    11281 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
    11341 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
    11401 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgccgg
    11461 cgttaactca agcgattaga tgcactaagc acataattgc tcacagccaa actatcaggt
    11521 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
    11581 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11641 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11701 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11761 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    11821 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11881 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11941 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12001 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12061 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    12181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    12241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    12301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    12361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    12421 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    12481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
    12541 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
    12601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    12661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
    12721 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
    12781 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
    12841 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
    12901 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
    12961 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
    13021 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
    13081 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
    13141 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
    13201 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
    13261 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
    13321 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
    13381 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
    13441 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
    13501 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
    13561 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
    13621 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
    13681 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
    13741 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
    13801 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
    13861 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
    13921 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
    13981 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
    14041 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
    14101 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
    14161 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
    14221 ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg ggggttccag
    14281 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    14341 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    14401 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    14461 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    14521 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    14581 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    14641 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    14701 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    14761 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    14821 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    14881 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    14941 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    15001 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    15061 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    15121 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    15181 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    15241 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    15301 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    15361 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    15421 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    15481 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    15541 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    15601 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    15661 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    15721 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    15781 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    15841 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    15901 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    15961 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    16021 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    16081 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    16141 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    16201 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    16261 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    16321 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    16381 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    16441 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtact
//
