LOCUS       Exported               10685 bp ds-DNA     circular SYN 21-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    114..138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     132..152
                     /label=SK017
     primer_bind     154..172
                     /label=YI102
     primer_bind     180..223
                     /label=YI052
     primer_bind     180..219
                     /label=YI069
     primer_bind     180..212
                     /label=Fragment 1.FOR
     promoter        200..446
                     /label=UBQ10 promoter
     misc_feature    209
                     /label=g
     primer_bind     complement(339..359)
                     /label=YI116
     3'UTR           447..531
                     /label=3'UTR UBQ10
     intron          532..835
                     /label=UBQ10 intron
     primer_bind     634..655
                     /label=YI221
     primer_bind     complement(831..860)
                     /label=YI070
     primer_bind     complement(833..853)
                     /label=YI053
     primer_bind     complement(836..860)
                     /label=Fragment 1.REV
     primer_bind     841..878
                     /label=Fragment 2.FOR
     primer_bind     841..873
                     /label=YI071
     primer_bind     847..878
                     /label=YI076
     5'UTR           847..850
     misc_feature    850
                     /label=u2-t
     CDS             851..1960
                     /codon_start=1
                     /label=SMT2
                     /translation="MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAE
                     KVQDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH
                     KDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRAR
                     LHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGS
                     MYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDL
                     ASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGET
                     GIFSPMHMILCRKPESPEESSDYKDDDDK"
     CDS             851..853
                     /codon_start=1
                     /label=PGM
                     /translation="M"
     misc_feature    853
                     /label=cvp1-3 G to A
     primer_bind     complement(887..909)
                     /label=YI427
     primer_bind     complement(928..947)
                     /label=Primer 2
     primer_bind     1010..1029
                     /label=YI520
     primer_bind     complement(1042..1061)
                     /label=YI521
     primer_bind     1136..1155
                     /label=YI428
     misc_feature    1369
                     /label=Deletion in frill-1
     primer_bind     complement(1468..1487)
                     /label=YI429
     primer_bind     1528..1547
                     /label=YI518
     primer_bind     1529..1548
                     /label=YI522
     primer_bind     complement(1565..1584)
                     /label=YI519
     primer_bind     complement(1588..1607)
                     /label=YI523
     primer_bind     complement(1918..1981)
                     /label=YI072
     primer_bind     complement(1918..1981)
                     /label=Fragment 2.REV
     CDS             1934..1957
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1955
                     /label=g
     centromere      1961
                     /label=3'UTR
     terminator      1966..2672
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2040..2059)
                     /label=YI201
     primer_bind     complement(2295..2312)
                     /label=YI223
     primer_bind     2495..2513
                     /label=YI216
     primer_bind     2604..2623
                     /label=SKC12
                     /note="LB primer pSKI015"
     primer_bind     complement(2666..2706)
                     /label=YI002
     primer_bind     complement(2773..2792)
                     /label=YI101
     primer_bind     2911..2934
                     /label=SL137
                     /note="to amplify citrine cds with XhoI site"
     CDS             complement(2912..3628)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2912..2929
                     /label=RGFP
                     /note="GFP"
     primer_bind     2924..2943
                     /label=LAP004
                     /note="pCF566 (Pair)"
     primer_bind     complement(2969..2988)
                     /label=OM102
     primer_bind     2971..2989
                     /label=LAP041
                     /note="pTP11 (Pair)"
     primer_bind     complement(3021..3040)
                     /label=LAP003
                     /note="pCF566 (Pair)"
     primer_bind     complement(3112..3131)
                     /label=LAP044
                     /note="citrine F"
     primer_bind     complement(3148..3166)
                     /label=LAP040
                     /note="pTP11 (Pair)"
     primer_bind     3174..3193
                     /label=MdW12
                     /note="genotyping CycB1"
     primer_bind     3178..3197
                     /label=LAP043
                     /note="citrine R"
     primer_bind     complement(3230..3249)
                     /label=LAP001
                     /note="venus"
     primer_bind     3303..3322
                     /label=LAP045
                     /note="citrine R"
     primer_bind     complement(3308..3327)
                     /label=LAP042
                     /note="citrine F"
     primer_bind     3328..3346
                     /label=LAP039
                     /note="pTP11 (Pair)"
     primer_bind     3478..3497
                     /label=OM134
                     /note="GFP RP"
     primer_bind     3492..3511
                     /label=PD 34 (2)
                     /note="CFP-YFP"
     primer_bind     3492..3511
                     /label=PD 34
                     /note="PCR/sequ of CFP or YFP clones,about 120bp 3' of the 
                     ATG"
     primer_bind     3544..3562
                     /label=LAP037
                     /note="qPCRAS2/FIL"
     primer_bind     4223..4250
                     /label=p745
     primer_bind     4223..4250
                     /label=OM122
                     /note="cca1-1 tDNA"
     primer_bind     4223..4245
                     /label=oASF169
     misc_feature    4264..4288
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4816..5607
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5851..6439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6625..6765
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      7109..7303
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7369..8436)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(8870..9499)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     10541..10560
                     /label=YI207
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctaagctt tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc
      241 cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa
      301 taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt
      361 attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc
      421 ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca
      481 atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc
      541 tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata
      601 tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt
      661 tagatatcat cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa
      721 tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagtt ctggtgttag
      781 tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagactag
      841 cccgcggaaa atggactctt taacactctt cttcaccggt gcactcgtcg ccgtcggtat
      901 ctactggttc ctctgcgttc tcggtccagc agagcgtaaa ggcaaacgag ccgtagatct
      961 ctctggtggc tcaatctccg ccgagaaagt ccaagacaac tacaaacagt actggtcttt
     1021 cttccgccgt ccaaaagaaa tcgaaaccgc cgagaaagtt ccagacttcg tcgacacatt
     1081 ctacaatctc gtcaccgaca tatacgagtg gggatgggga caatccttcc acttctcacc
     1141 atcaatcccc ggaaaatctc acaaagacgc cacgcgcctc cacgaagaga tggccgtaga
     1201 tctgatccaa gtcaaacctg gtcaaaagat cctagacgtc ggatgcggtg tcggcggtcc
     1261 gatgcgagcg attgcatctc actcgcgagc taacgtagtc gggattacaa taaacgagta
     1321 tcaggtgaac agagctcgtc tccacaataa gaaagctggt ctcgacgcgc tttgcgaggt
     1381 cgtgtgtggt aacttcctcc agatgccgtt cgatgacaac agtttcgacg gtgcttattc
     1441 catcgaagcc acgtgtcacg cgccgaagct ggaagaagtg tacgcagaga tctacagggt
     1501 gttgaaaccc ggatctatgt atgtgtcgta cgagtgggtt acgacggaga aatttaaggc
     1561 ggaggatgac gaacacgtgg aggtaatcca agggattgag agaggcgatg cgttaccagg
     1621 gcttagggct tacgtggata tagctgagac ggctaaaaag gttgggtttg agatagtgaa
     1681 ggagaaggat ctggcgagtc caccggctga gccgtggtgg actaggctta agatgggtag
     1741 gcttgcttat tggaggaatc acattgtggt tcagattttg tcagcggttg gagttgctcc
     1801 taaaggaact gttgatgttc atgagatgtt gtttaagact gctgattatt tgaccagagg
     1861 aggtgaaacc ggaatattct ctccgatgca tatgattctc tgcagaaaac cggagtcacc
     1921 ggaggagagt tctgattaca aggacgacga tgacaagtga ggatcctgct ttaatgagat
     1981 atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa
     2041 aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata
     2101 tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtacc
     2161 ctactactta tatgtacaat attaaaatga aaacaatata ttgtgctgaa taggtttata
     2221 gcgacatcta tgatagagcg ccacaataac aaacaattgc gttttattat tacaaatcca
     2281 attttaaaaa aagcggcaga accggtcaaa cctaaaagac tgattacata aatcttattc
     2341 aaatttcaaa aggccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     2401 ttcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     2461 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     2521 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     2581 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     2641 gaattttgcg acaacatgtc gaggctcagc aggacctcta gagcgccact ggattttggt
     2701 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     2761 tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaattcc cttatctggg
     2821 aactactcac acattattat agagagagat agatttgtag agagagactg gtgatttcag
     2881 cgatgcagcc gggcggcgct tacagctcgt ccttgtacag ctcgtccatg ccgagagtga
     2941 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     3001 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     3061 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     3121 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     3181 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     3241 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     3301 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     3361 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     3421 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     3481 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     3541 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     3601 tgaacagctc ctcgcccttg ctcaccatgt cggccgagga taatgatagg agaagtgaaa
     3661 agatgaaaaa gagaaaaaga ttagtcttca ccatggctat cgttcgtaaa tggtgaaaat
     3721 tttcagaaaa tagcttttgc tttaaaagaa atgatttaaa ttgctgcaat agaagtagaa
     3781 tgcttgattg cttgagattc gtttgttttg tatatgttgt gttgagaatt cgcccttgtt
     3841 ttgctatttg tgtatgtttt cttgttttgg tttgagtgaa gagtgtagtg aagggttggt
     3901 ttgtatttat aggtgagaaa ggagatttgc atggcaatca cgtgtaagaa tgcatgcatg
     3961 catgagtgtg tgtggagaga gagagatgct cttacgttgt tgtcgggatt atgtgttgga
     4021 aaagagtgac aagtgacgaa caaaacaata ccactcatca tagctccgct tatgcatgct
     4081 atgtgtgatt tgtagctctt gttctgacat gacactatga acaatttttt aaaaagaagg
     4141 ttaaactttt atttaaataa aaaccctatg ttaatttggt ttcaagggcg aattcaattc
     4201 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     4261 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     4321 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     4381 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     4441 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     4501 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctcgt
     4561 tcggttcgta agctgtaatg caagtagcgt aactgccgtc acgcaactgg tccagaacct
     4621 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttct tgttatgaca
     4681 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     4741 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     4801 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     4861 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     4921 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     4981 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     5041 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     5101 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     5161 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     5221 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     5281 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     5341 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     5401 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     5461 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     5521 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     5581 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     5641 cttcgccggc gttaactcaa gcgattagat gcactaagca cataattgct cacagccaaa
     5701 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     5761 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5821 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5881 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5941 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6001 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6061 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6121 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6181 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6241 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6301 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6361 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6421 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6481 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6541 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6601 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6661 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6721 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6781 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6841 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6901 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     6961 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     7021 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     7081 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     7141 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     7201 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     7261 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     7321 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     7381 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     7441 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     7501 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7561 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7621 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7681 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7741 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7801 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     7861 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     7921 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     7981 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     8041 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     8101 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     8161 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     8221 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     8281 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     8341 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     8401 cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca cgccgattcc tcgggcttgg
     8461 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     8521 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     8581 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     8641 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     8701 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     8761 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     8821 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     8881 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     8941 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     9001 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
     9061 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
     9121 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
     9181 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
     9241 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
     9301 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
     9361 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
     9421 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
     9481 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
     9541 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
     9601 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
     9661 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
     9721 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
     9781 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
     9841 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
     9901 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
     9961 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    10021 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    10081 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    10141 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    10201 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    10261 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    10321 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    10381 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    10441 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    10501 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    10561 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    10621 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    10681 gtact
//
