LOCUS       Exported               11128 bp ds-DNA     circular SYN 21-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    114..138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     132..152
                     /label=SK017
     primer_bind     154..172
                     /label=YI102
     primer_bind     180..218
                     /label=YI011
     promoter        201..1293
                     /label=FRO6
     primer_bind     complement(477..498)
                     /label=YI117
     primer_bind     1101..1122
                     /label=YI224
     primer_bind     complement(1264..1303)
                     /label=YI073
     primer_bind     1284..1320
                     /label=YI074
     CDS             1294..2403
                     /codon_start=1
                     /label=SMT2
                     /translation="MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAE
                     KVQDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH
                     KDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRAR
                     LHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGS
                     MYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDL
                     ASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGET
                     GIFSPMHMILCRKPESPEESSDYKDDDDK"
     primer_bind     1294..1321
                     /label=YI076
     CDS             1294..1296
                     /codon_start=1
                     /label=PGM
                     /translation="M"
     misc_feature    1296
                     /label=cvp1-3 G to A
     primer_bind     complement(1330..1352)
                     /label=YI427
     primer_bind     complement(1371..1390)
                     /label=Primer 2
     primer_bind     1453..1472
                     /label=YI520
     primer_bind     complement(1485..1504)
                     /label=YI521
     primer_bind     1579..1598
                     /label=YI428
     misc_feature    1812
                     /label=Deletion in frill-1
     primer_bind     complement(1911..1930)
                     /label=YI429
     primer_bind     1971..1990
                     /label=YI518
     primer_bind     1972..1991
                     /label=YI522
     primer_bind     complement(2008..2027)
                     /label=YI519
     primer_bind     complement(2031..2050)
                     /label=YI523
     primer_bind     complement(2361..2424)
                     /label=YI072
     CDS             2377..2400
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2398
                     /label=g
     centromere      2404
                     /label=3'UTR
     terminator      2409..3115
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2483..2502)
                     /label=YI201
     primer_bind     complement(2738..2755)
                     /label=YI223
     primer_bind     2938..2956
                     /label=YI216
     primer_bind     3047..3066
                     /label=SKC12
                     /note="LB primer pSKI015"
     primer_bind     complement(3109..3149)
                     /label=YI002
     primer_bind     complement(3216..3235)
                     /label=YI101
     primer_bind     3354..3377
                     /label=SL137
                     /note="to amplify citrine cds with XhoI site"
     CDS             complement(3355..4071)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3355..3372
                     /label=RGFP
                     /note="GFP"
     primer_bind     3367..3386
                     /label=LAP004
                     /note="pCF566 (Pair)"
     primer_bind     complement(3412..3431)
                     /label=OM102
     primer_bind     3414..3432
                     /label=LAP041
                     /note="pTP11 (Pair)"
     primer_bind     complement(3464..3483)
                     /label=LAP003
                     /note="pCF566 (Pair)"
     primer_bind     complement(3555..3574)
                     /label=LAP044
                     /note="citrine F"
     primer_bind     complement(3591..3609)
                     /label=LAP040
                     /note="pTP11 (Pair)"
     primer_bind     3617..3636
                     /label=MdW12
                     /note="genotyping CycB1"
     primer_bind     3621..3640
                     /label=LAP043
                     /note="citrine R"
     primer_bind     complement(3673..3692)
                     /label=LAP001
                     /note="venus"
     primer_bind     3746..3765
                     /label=LAP045
                     /note="citrine R"
     primer_bind     complement(3751..3770)
                     /label=LAP042
                     /note="citrine F"
     primer_bind     3771..3789
                     /label=LAP039
                     /note="pTP11 (Pair)"
     primer_bind     3921..3940
                     /label=OM134
                     /note="GFP RP"
     primer_bind     3935..3954
                     /label=PD 34 (2)
                     /note="CFP-YFP"
     primer_bind     3935..3954
                     /label=PD 34
                     /note="PCR/sequ of CFP or YFP clones,about 120bp 3' of the 
                     ATG"
     primer_bind     3987..4005
                     /label=LAP037
                     /note="qPCRAS2/FIL"
     primer_bind     4666..4693
                     /label=p745
     primer_bind     4666..4693
                     /label=OM122
                     /note="cca1-1 tDNA"
     primer_bind     4666..4688
                     /label=oASF169
     misc_feature    4707..4731
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5259..6050
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6294..6882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7068..7208
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      7552..7746
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7812..8879)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9313..9942)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     10984..11003
                     /label=YI207
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctaagctt cgatgctctc aaggccaaag aacattatgg aaattcgaag
      241 atatattatc gatgcatata atttatataa tcacagtctg tatgtattat ggaaacaact
      301 ttaactaatt tttgagattg atgctacttc atgatagcaa tttcttagtt cttttgagtt
      361 aataacttaa tatataaata taatggtaca acaaccatta caatcgtcat tataatcatc
      421 ttatccatac tcaatagtca atactctcac cttccctaaa gtgtggttga aatcaaactt
      481 tactctctct aggcctgatt ataattcttt tttgtctaca catacaactt catgagactt
      541 aattataatt tcttagtgtt cttttgttga catgtaaatc gtagctaagt ctaagtggta
      601 cttttatttg ttattggatt ttgtttcggt tacttaaaag tgtaatgaag aatttttttg
      661 actatataca tctaagtttt tccttggact gattataact tgtgtgttcg agattgatac
      721 aacttcatga tagtcgatta ttcttgttta acgtaacaca ttgttagata gtgtcattta
      781 ccagaaacaa tctaacagtg aaactttgca aatttatctc tctgctggat cttgaacgcg
      841 gaaaccgaat aatgtttctt tcgagctaat ccgaatgatg ttgaaattct atatgttcag
      901 attcaggtaa tgtaagctta tccaataacc atcatagtcg tcataataac catcttatct
      961 atactaaagt agtaagtact ctcagtctct caccttaccc ttaattgtgg ttgaaatcaa
     1021 cctaacgtga cactctctcc caagtacgtt actctcacgt gttaatgaca ctctcatttt
     1081 tttttatagt tttttttatt gtgtactagg attgttgtcc gaaggaatct ttcataaatg
     1141 agaatgatag aaacctcaat ggtccagaaa attttacttg actcttctaa gaatatctta
     1201 ttagtttatc tctagtaacg ccaccttaaa agccgatgct gcttctgagc caacttctca
     1261 tcacaacact gagaagtgga aattcaaata aagatggact ctttaacact cttcttcacc
     1321 ggtgcactcg tcgccgtcgg tatctactgg ttcctctgcg ttctcggtcc agcagagcgt
     1381 aaaggcaaac gagccgtaga tctctctggt ggctcaatct ccgccgagaa agtccaagac
     1441 aactacaaac agtactggtc tttcttccgc cgtccaaaag aaatcgaaac cgccgagaaa
     1501 gttccagact tcgtcgacac attctacaat ctcgtcaccg acatatacga gtggggatgg
     1561 ggacaatcct tccacttctc accatcaatc cccggaaaat ctcacaaaga cgccacgcgc
     1621 ctccacgaag agatggccgt agatctgatc caagtcaaac ctggtcaaaa gatcctagac
     1681 gtcggatgcg gtgtcggcgg tccgatgcga gcgattgcat ctcactcgcg agctaacgta
     1741 gtcgggatta caataaacga gtatcaggtg aacagagctc gtctccacaa taagaaagct
     1801 ggtctcgacg cgctttgcga ggtcgtgtgt ggtaacttcc tccagatgcc gttcgatgac
     1861 aacagtttcg acggtgctta ttccatcgaa gccacgtgtc acgcgccgaa gctggaagaa
     1921 gtgtacgcag agatctacag ggtgttgaaa cccggatcta tgtatgtgtc gtacgagtgg
     1981 gttacgacgg agaaatttaa ggcggaggat gacgaacacg tggaggtaat ccaagggatt
     2041 gagagaggcg atgcgttacc agggcttagg gcttacgtgg atatagctga gacggctaaa
     2101 aaggttgggt ttgagatagt gaaggagaag gatctggcga gtccaccggc tgagccgtgg
     2161 tggactaggc ttaagatggg taggcttgct tattggagga atcacattgt ggttcagatt
     2221 ttgtcagcgg ttggagttgc tcctaaagga actgttgatg ttcatgagat gttgtttaag
     2281 actgctgatt atttgaccag aggaggtgaa accggaatat tctctccgat gcatatgatt
     2341 ctctgcagaa aaccggagtc accggaggag agttctgatt acaaggacga cgatgacaag
     2401 tgaggatcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca
     2461 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
     2521 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
     2581 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat
     2641 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat
     2701 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa
     2761 gactgattac ataaatctta ttcaaatttc aaaaggcccc aggggctagt atctacgaca
     2821 caccgagcgg cgaactaata acgttcactg aagggaactc cggttccccg ccggcgcgca
     2881 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc
     2941 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag
     3001 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac
     3061 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct
     3121 ctagagcgcc actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
     3181 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa
     3241 gaaccctaat tcccttatct gggaactact cacacattat tatagagaga gatagatttg
     3301 tagagagaga ctggtgattt cagcgatgca gccgggcggc gcttacagct cgtccttgta
     3361 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     3421 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     3481 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     3541 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     3601 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     3661 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     3721 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     3781 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     3841 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     3901 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     3961 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     4021 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggccga
     4081 ggataatgat aggagaagtg aaaagatgaa aaagagaaaa agattagtct tcaccatggc
     4141 tatcgttcgt aaatggtgaa aattttcaga aaatagcttt tgctttaaaa gaaatgattt
     4201 aaattgctgc aatagaagta gaatgcttga ttgcttgaga ttcgtttgtt ttgtatatgt
     4261 tgtgttgaga attcgccctt gttttgctat ttgtgtatgt tttcttgttt tggtttgagt
     4321 gaagagtgta gtgaagggtt ggtttgtatt tataggtgag aaaggagatt tgcatggcaa
     4381 tcacgtgtaa gaatgcatgc atgcatgagt gtgtgtggag agagagagat gctcttacgt
     4441 tgttgtcggg attatgtgtt ggaaaagagt gacaagtgac gaacaaaaca ataccactca
     4501 tcatagctcc gcttatgcat gctatgtgtg atttgtagct cttgttctga catgacacta
     4561 tgaacaattt tttaaaaaga aggttaaact tttatttaaa taaaaaccct atgttaattt
     4621 ggtttcaagg gcgaattcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     4681 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     4741 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     4801 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     4861 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     4921 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg
     4981 aacccagtgg acataagcct cgttcggttc gtaagctgta atgcaagtag cgtaactgcc
     5041 gtcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     5101 tttcatggct tcttgttatg acatgttttt ttggggtaca gtctatgcct cgggcatcca
     5161 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     5221 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     5281 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     5341 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     5401 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     5461 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     5521 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     5581 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     5641 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     5701 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     5761 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     5821 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     5881 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     5941 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     6001 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
     6061 gaaattcgtt caagccgacg ccgcttcgcc ggcgttaact caagcgatta gatgcactaa
     6121 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     6181 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt
     6241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6421 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6901 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6961 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7021 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7081 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     7141 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     7201 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7261 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7321 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     7381 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     7441 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     7501 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     7561 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     7621 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     7681 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     7741 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     7801 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     7861 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     7921 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     7981 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     8041 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     8101 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     8161 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     8221 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     8281 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     8341 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     8401 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     8461 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     8521 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     8581 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
     8641 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
     8701 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
     8761 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
     8821 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
     8881 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
     8941 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9001 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9061 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     9121 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
     9181 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
     9241 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
     9301 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
     9361 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
     9421 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
     9481 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
     9541 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
     9601 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
     9661 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
     9721 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
     9781 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
     9841 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
     9901 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
     9961 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10021 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10081 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    10141 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    10201 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    10261 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    10321 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    10381 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    10441 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    10501 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    10561 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    10621 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    10681 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    10741 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    10801 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    10861 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    10921 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    10981 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11041 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    11101 agcagcggag gggttggatc aaagtact
//
