LOCUS       Exported                4510 bp ds-DNA     circular SYN 16-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          623..628
     misc_feature    1..130
                     /label=ITR
     misc_feature    150..608
                     /label=hSyn1 Promoter
     misc_feature    609..622
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_G_EGFP-N"
     misc_feature    609..622
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_G_EGFP-N"
     misc_feature    623..628
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_R_140619_Seq_AAV_Dlx_R"
     misc_feature    623..628
                     /label=Gibs_Flex_Fb
     protein_bind    629..662
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    713..746
                     /label=F5
     misc_feature    758..814
                     /label=MCS
                     /note=" multiple cloning site
                     "
     protein_bind    complement(825..858)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    complement(909..942)
                     /label=F5
     misc_feature    943..950
                     /label=AscI
     misc_feature    951..1539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    1547..1771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1773..1913
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      1988..2443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2725..2829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2830..3690
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3861..4449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gctagactgc agagggccct gcgtatgagt gcaagtgggt tttaggacca
      181 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc
      241 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc
      301 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg
      361 gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc
      421 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac
      481 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg
      541 gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag
      601 cgcagtcaga tatcgtttaa actccggaga agttcctatt ctctagaaag tataggaact
      661 tcgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct tagaagttcc
      721 tattcttcaa aaggtatagg aacttcaacg cgccctagaa ttcgagctcg gtacccgggg
      781 atcctctaga gtcgacctgc aggcatgcaa gcttggtggc tagcgaagtt cctatacttt
      841 ctagagaata ggaacttctt gccttaaccc agaaattatc actgttattc tttagaatgg
      901 tgcaaagaga agttcctata ccttttgaag aataggaact tcggcgcgcc aatcaacctc
      961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     1141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     1201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     1261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     1321 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     1381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     1441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     1501 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggatcctgt gccttctagt
     1561 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1621 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     1681 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     1741 aggcatgctg gggatgcggt gggctctatg gcaggaaccc ctagtgatgg agttggccac
     1801 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     1861 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     1921 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     1981 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     2221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     2341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2401 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     2461 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     2521 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2581 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     2641 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2701 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2761 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2821 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2881 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2941 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3001 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3061 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3121 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3181 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3241 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3301 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3361 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3421 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3481 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3541 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3601 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3661 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3721 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3781 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3841 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3901 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3961 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4021 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4081 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4141 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4201 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4261 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4321 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4381 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4441 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4501 gctcacatgt
//
