LOCUS       Exported                6849 bp ds-DNA     circular SYN 30-JUL-2020
DEFINITION  rabphilin-3A isoform X1 [Bos taurus]G309E.
ACCESSION   XP_024832776
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     T65G, Y65G mutationA206K mutation
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
     primer_bind     571..589
                     /label=pEGFP-FP
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mdEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGGGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(621..638)
                     /label=pEGFP-RP
     primer_bind     complement(658..679)
                     /label=EGFP-N
     primer_bind     complement(658..674)
                     /label=pEGFP_N
     primer_bind     complement(664..679)
                     /label=EGFP-NR
     primer_bind     1266..1287
                     /label=EGFP-C
     primer_bind     complement(1302..1318)
                     /label=EGFP-CR
     CDS             1345..3477
                     /codon_start=1
                     /label=Rabphilin
                     /translation="MTDTVFSSSSSRWMCPSDRPLQSNDKEQLQTGWSVHPSGQPDRQR
                     KQEELTDEEKEIINRVIARAEKMEEMEQERIGRLVDRLENMRKNVAGDGVNRCILCGEQ
                     LGMLGSACVVCEDCKKNVCTKCGVETSNNRPHPVWLCKICIEQREVWKRSGAWFFKGFP
                     KQVLPQPMPIKKNKPQQPVSEPVPAAPEQPTPEPKHPARAPTRVFSLSAGDTEDRRGPG
                     QKTGPDMTSAPGRGSYGPPVRRASEARMSSSGRDSDSWDQGHGMAAGDPSQSPAGLRRA
                     NSVQASRPAPASMQSPAPPQPGQPGPPEGSRPSPGPTGRFPDQRPEVAPSDPDYTGAAA
                     QPREERTGGIGGYSAAGTREDRAGHPPGSYTQASAAAPQPVVASARQPPPPEEDEEEAN
                     SYDSDEATTLGALEFSLLYDQDNSSLHCTIIKAKGLKPMDSNGLADPYVKLHLLPGASK
                     SNKLRTKTLRNTRNPIWNETLVYHGITDEDMQRKTLRISVCDEDKFGHNEFIGETRFSL
                     KKLKPNQRKNFNICLERVIPMKRAGTTGSARGMALYEEEQVERIGDIEERGKILVSLMY
                     STQQGGLIVGIIRCVHLAAMDANGYSDPFVKLWLKPDMGKKAKHKTQIKKKTLNPEFNE
                     EFFYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGISAKGERLKHWYECLKNKDKK
                     IERWHQLQNENHVSSD"
     primer_bind     complement(3537..3556)
                     /label=pFastBac_Reverse
     primer_bind     complement(3543..3562)
                     /label=SV40-pArev
     polyA_signal    3637..3758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3728..3747
                     /label=EBV-RP
     rep_origin      complement(3765..4220)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4221..4238)
                     /label=pQE-F
     primer_bind     complement(4221..4237)
                     /label=QE_Promoter
     promoter        4247..4351
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4353..4710
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4545..4564
                     /label=SV40-Promoter
     rep_origin      4561..4696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4745..5539
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5771..5818
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6147..6735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctggga ggtggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatgact gacaccgtgt tcagcagcag ctcaagccgt
     1381 tggatgtgtc ccagtgaccg acccctgcag tcaaatgata aagagcagct ccagacagga
     1441 tggtctgtcc accccagcgg tcagccggac agacagagga aacaggaaga gctgacagat
     1501 gaggagaagg aaatcatcaa cagggtgatc gctcgcgctg agaagatgga agagatggaa
     1561 caggagcgaa tcgggcgcct ggtggaccgc ctagagaaca tgaggaagaa cgtggccggg
     1621 gacggggtga accgctgcat cctgtgtgga gaacagctgg ggatgctggg ctctgcctgt
     1681 gtggtgtgtg aggactgtaa gaagaatgtc tgcaccaagt gtggggtgga gacctccaac
     1741 aaccgcccac accctgtgtg gctctgcaaa atctgcatcg agcagagaga ggtgtggaag
     1801 cgctctggag catggttctt caagggcttc cccaaacagg tcctcccaca gcctatgccc
     1861 ataaagaaga acaagcccca gcagccagtc agtgagcctg tgcccgctgc ccctgagcag
     1921 cccactcctg agcccaagca ccctgcccgg gctccaactc gagttttttc tctctctgca
     1981 ggtgacactg aagacaggag gggcccaggt cagaagacag gccctgacat gacttctgct
     2041 cctgggcgag gaagctacgg gcctcctgtg cgcagggcct ctgaggcacg aatgagctcc
     2101 tctggccggg actcagacag ctgggaccag ggccacggca tggctgctgg ggaccccagc
     2161 cagagcccag caggtttgag acgggccaac tcagtccagg cttctagacc tgccccagcc
     2221 tcaatgcaga gcccggcacc tccccaaccg gggcaaccag gacccccaga aggcagcaga
     2281 cccagtccgg ggccgacggg acgctttcca gatcagagac cagaggtggc tcccagtgac
     2341 cccgactata caggggccgc cgcccaaccc cgggaggaga gaaccggggg aatcggaggc
     2401 tactcggcag ctggaaccag ggaggaccga gcgggccacc ccccgggctc ctatacccaa
     2461 gcctctgcgg ctgctcccca gccggtggtg gcctcggccc gtcagccccc accccctgag
     2521 gaggacgagg aggaagctaa cagctacgat tcggatgaag caaccaccct gggtgccctg
     2581 gagttcagcc ttctctatga ccaggacaac agctccctgc actgcaccat cataaaggcc
     2641 aagggactga agcccatgga ttcgaatggc ttggccgatc cctacgttaa gctgcacctc
     2701 ctgccgggag ccagcaagtc caacaagctt cgtacaaaaa ccctgcggaa caccagaaac
     2761 cccatctgga acgaaactct ggtctaccat ggcatcacag acgaggacat gcaaaggaag
     2821 accctcagaa tctctgtctg tgatgaggac aaattcggcc ataatgagtt tattggtgag
     2881 accagattct ccctcaagaa actgaagccc aatcagagga agaacttcaa catctgtctg
     2941 gagcgggtga tcccgatgaa gcgtgccgga accactgggt cagcccgagg catggccctc
     3001 tatgaggagg agcaggtgga acgtattggt gacatagagg aacgcggcaa gatcctggtg
     3061 tccctcatgt acagcacaca gcagggaggc ctcatcgtgg gcatcatacg ctgcgtgcac
     3121 ctggcggcca tggatgccaa tggctactca gatccatttg tcaagctctg gctgaaacca
     3181 gacatgggaa agaaagccaa acacaagact caaattaaga agaaaacctt gaatcctgaa
     3241 tttaatgagg aatttttcta tgacatcaaa cacagtgacc tggcgaagaa gtcactggac
     3301 atctcagtct gggactatga catcggcaag tccaatgatt acattggagg ctgccagctg
     3361 gggatctcgg ccaagggaga gcgcttaaaa cactggtacg agtgtctgaa aaacaaggac
     3421 aagaagatcg aacgctggca ccagctacag aacgagaacc acgtgtcgag cgattagaat
     3481 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     3541 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3601 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3721 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     3781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4201 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4321 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     4381 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4501 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4561 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4621 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4681 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4741 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4801 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4861 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4921 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4981 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5041 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5101 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5161 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5221 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     5281 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5341 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5401 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5461 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5521 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5581 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5641 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5701 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5761 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5821 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5881 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5941 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     6001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6781 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6841 gccatgcat
//
