LOCUS       Exported                2547 bp ds-DNA     circular SYN 26-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        25..462
                     /label=35S-oTMV
     misc_feature    238
                     /label=C to T to remove BbsI restriction site
     misc_feature    253
                     /label=T to C to remove BbsI restriction site
     terminator      504..561
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(756..1344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1527..1621
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1642..2301)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2485..2528
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tagagcgatg agtggtctcg ggagtgagac ttttcaacaa agggtaatat cgggaaacct
       61 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      121 tggctcctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac
      181 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagatgt
      241 tccaaccacg tcctcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      301 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      361 ggagaggaca ggcttcttga gatccttcaa caattaccaa caacaacaaa caacaaacaa
      421 cattacaatt actatttaca attacagtcg actctagagg ataatgtgag accactcatc
      481 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      541 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1501 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1561 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1621 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa
     1681 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1741 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1801 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1861 ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1921 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1981 actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2041 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     2101 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2161 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2221 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2281 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     2341 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     2401 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2461 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2521 atgatttctg gaattcgcgg ccgcttc
//
