LOCUS       Exported                7629 bp ds-DNA     circular SYN 27-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 28, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        22..386
                     /label=AtNRP
     CDS             388..2016
                     /codon_start=1
                     /label=E-Luc
                     /translation="MEREKNVVYGPEPKHPLGNFTAGEMLYNALHKHSHIPQAILDVMG
                     NESLSYQEFFDTTVKLGQSLQNCGYKMNDVVSICAENNKRFFIPIISAWYIGMVVAPVN
                     EDYIPDELCKVTGISKPILVFTTRKILPKVLEVKDRTNYIKRIIILDSEENLLGCESLH
                     NFMSRYSDNNLQTFKPLHYDPVDQVAAILCSSGTTGLPKGVMQTHRNICVRLTHASDPR
                     VGTQLIPGVSVLAYLPFFHAFGFSINLGYFMVGLRVVMLRRFNQEVFLKAIQDYEVRSV
                     INVPSTILFLSKSPLVDKYDLSTLAELCCGAAPLAKEVAEIAVKRLNLPGIRCGYGLTE
                     STSANIHTLHNEFKSGSLGKVTPYMAAKIIDRNTGEALGPNQVGELCIWGPMVTKGYVN
                     NPQATKEAIDDDGWLHSGDFGYYDEDEYFYIVDRYKELIKYKGYQVAPVELEEILLQHP
                     GIRDVAVVGIPDIEAGELPAGFVVKQPGAQLTAKEVYDFLAQRVSHSKYLRGGVRFVDS
                     IPRNVTGKISRKELREALMEKASKL"
     misc_feature    760..762
                     /label=TTC to TTT to remove BbsI restriction site
     terminator      2021..2270
                     /label=AtHSP18.2 terminator
                     /label=Hsp18-2 ter
     promoter        2283..2573
                     /label=pNOS
     gene            2575..4221
                     /label=RedF Luciferase
     terminator      4226..4717
                     /label=NOS terminator
     misc_feature    4525
                     /label=C to T to remove BbsI restriction site
     misc_feature    4739..4763
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4854..5442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5613..6404)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7048..7483
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(7610..7629,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
ORIGIN
        1 taacggatcc ggtctcagga gcgaaaattc aaacaagtga caaaaataat attacgaccc
       61 gtccctttgt ttggttgccc cgttcgtgta tttgcttggc cgttggtgtt cccatgcgcc
      121 acttagcctc caaagtcttc tctctaaact ccttttttat accataatct ctgtttagtt
      181 tatacttaat tgcgctttat cctgcagtaa acacaattta aatagtttca aataaattta
      241 gaaagaataa aacaaataga aatcagaagg tgtctgtttc ctcctcgcaa catacgatca
      301 aagagaaaca acttgaccct ttacattgct caagagctgc aagacccttc ctctatataa
      361 ggaagttcat ttcatttgga gaggaaaatg gagagagaga agaacgtggt gtacggcccc
      421 gagcccaagc accctctggg caacttcacc gccggcgaga tgctgtacaa cgctctgcac
      481 aagcactccc acatccccca ggccatcctg gacgtgatgg gcaacgagtc cctttcctac
      541 caggagttct tcgacactac tgtgaagctg ggccagagcc tccagaactg tggctacaag
      601 atgaacgatg tcgtgtcgat ctgtgcagag aacaacaaga gattcttcat ccccatcatc
      661 tccgcctggt acatcggcat ggtggtggcc cctgtgaacg aggactatat cccagacgag
      721 ctgtgtaaag tgaccggcat ctccaagccg atcctggtct ttaccactag gaagatcctg
      781 cctaaggttt tggaggttaa agacagaacc aactacataa agaggatcat catactggac
      841 tctgaagaga acctgctggg ctgcgagagc ctgcacaact tcatgtccag gtactccgac
      901 aacaacctcc aaacattcaa gcctctgcac tacgaccctg tggaccaggt agccgccatc
      961 ctgtgctcct ccggcacaac cggcctgcct aaaggcgtga tgcagaccca caggaacatc
     1021 tgtgtgagac tcacacacgc atctgacccc agagtgggta cacaactcat ccccggcgta
     1081 tccgtgctgg cctacctgcc attcttccac gccttcggct tcagtatcaa cctgggctat
     1141 ttcatggtgg gcctgagagt ggtgatgctc cgaaggttta accaggaggt gttcctgaag
     1201 gccatccagg actacgaggt gaggagcgtg atcaacgttc cctccacaat cctgttcctg
     1261 tccaagagcc ctctggtgga caagtacgac ctatccaccc tggcggagct gtgctgtgga
     1321 gccgctcctc tggcgaagga ggtggccgag atcgccgtga agaggctgaa cctgccaggg
     1381 atacggtgtg gctacggtct aacagagtct acctccgcca acatccatac tctgcacaac
     1441 gagttcaagt ccggctccct gggcaaggtg acaccttaca tggccgccaa gatcatcgac
     1501 aggaacaccg gcgaggccct gggtccaaac caggtgggcg agctgtgcat ctggggacct
     1561 atggtaacaa aaggctatgt gaacaaccca caggctacta aggaggccat cgacgacgac
     1621 ggctggctgc actctggcga cttcggctac tacgacgagg acgagtattt ctacatcgtg
     1681 gaccggtaca aggagctgat caaatacaag ggctatcagg tcgcccctgt ggagctggag
     1741 gagatcctcc ttcagcaccc aggcatcagg gacgtggccg tcgtgggtat ccctgacatc
     1801 gaggccggcg agctgccagc cggcttcgtg gtgaagcagc ccggcgccca actcaccgct
     1861 aaggaggtgt acgacttcct ggcccagagg gtgtctcact ccaagtacct gaggggcggc
     1921 gtaaggttcg tggactctat ccccaggaac gtgacaggca agattagtcg aaaagagctg
     1981 agggaggccc tgatggagaa ggcttctaag ctgtaagctt atatgaagat gaagatgaaa
     2041 tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt
     2101 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatctc attataatga
     2161 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat
     2221 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag cgctgtcagg
     2281 agatacatga gaattaaggg agtcacggta tgacccccgc cgatgacgcg ggacaagccg
     2341 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct
     2401 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
     2461 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
     2521 ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga ggcaatggag
     2581 aacatggaga acgacgagaa cattgtggtg ggacccaagc cgttctaccc gatcgaggag
     2641 ggttccgctg gcacccagct gcgcaagtac atggagcgct acgctaagct gggtgctatc
     2701 gccttcacca acgccgtgac cggtgtggat tactcctacg ccgagtacct ggagaagtcc
     2761 tgctgcctcg gaaaggccct gcagaactac ggtctggtcg tggatggccg cattgccctc
     2821 tgctcggaga actgcgagga gttcttcatt cccgtgatcg ctggtctgtt catcggagtc
     2881 ggcgttgccc caaccaacga gatctacacc ctgcgcgagc tggtccacag cctgggtatc
     2941 agcaagccga ccatcgtttt ctcgagcaag aagggcctcg acaaggttat caccgtgcaa
     3001 aaaaccgtca ccaccatcaa gaccatcgtt atcctggatt ccaaggttga ctaccgcggt
     3061 taccagtgcc tcgacacctt catcaagcgt aacaccccac ccggcttcca agcctcctcc
     3121 ttcaaaaccg tggaggttga tcgcaaggag caggtggccc tgatcatgaa ctcctccggc
     3181 tccaccggtc tgccaaaggg cgtgcagctg acccacgaga acaccgtcac ccgcttctcg
     3241 catgcccgtg atcccatcta cggcaaccag gtgtccccag gcaccgccgt cctgaccgtc
     3301 gtgcccctcc accacggctt cggcatgttc accactctcg gctacttgat ctgcggcttc
     3361 cgtgtggtga tgctgactaa gttcgacgag gagactttcc tcaagaccct gcaagactac
     3421 aagtgcactt acgtgatcct ggtgccgacc ctgttcgcca tcctgaacaa gagcgagctg
     3481 ttgaacaaat acgacctgtc gaacctggtg gagatcgctt ccggcggcgc cccactgtcg
     3541 aaggaagttg gcgaggccgt tgctcgccgc ttcaacctgc ccggcgtccg tcagggttac
     3601 ggcctgaccg agactacctc ggctatcatc atcacccccg agggcgacga taagcccggc
     3661 gcttccggca aggttgttcc actcttcaaa gccaaggtga tcgacctgga caccaagaag
     3721 tccctgggtc ccaaccgtcg cggagaagtg tgcgtcaagg gccccatgct gatgaagggc
     3781 tacgtcaaca acccagaggc caccaaggag ttgatcgacg aggagggctg gctgcacact
     3841 ggtgacatcg gttactacga cgaagagaag cacttcttca tcgttgaccg cctgaagtcc
     3901 ttgatcaagt acaagggcta ccaggttccc cccgccgagc tcgagagcgt cctgctgcag
     3961 cacccctcca ttttcgatgc cggagtggcc ggtgtccccg atcccgtggc tggcgagctg
     4021 ccaggtgccg tcgtcgtgct ggagtccggc aagaacatga ccgagaagga agtgatggat
     4081 tacgtggcct cgcaagtgtc caacgccaag cgcctgcgcg gcggcgtgcg cttcgtggat
     4141 gaggtgccca agggcctcac cggaaagatc gacggtcgcg ctatccgcga gatcctcaag
     4201 aagcccgtgg ccaagatgtg agcttggaat ggatcttcga tcccgatcgt tcaaacattt
     4261 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt atcatataat
     4321 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     4381 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     4441 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     4501 aattgccaag ctaattcttg aagatgaaag ggcctcgtga tacgcctatt tttataggtt
     4561 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4681 taaccctgat aaatgcttca ataatgggac cgactcgcgc tgtcatgaga ccggatcctg
     4741 acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga
     4801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5341 agaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5581 taaagtatat atgtgtaaca ttggtctagt gattatttgc cgactacctt ggtgatctcg
     5641 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     5701 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     5761 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     5821 taccaaatgc gggacaacgt aagcactaca tttcgctcat caccagccca gtcgggcggc
     5881 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     5941 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     6001 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     6061 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     6121 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     6181 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     6241 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     6301 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     6361 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt
     6421 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     6481 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
     6541 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     6601 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct
     6661 tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     6721 ggcagcagcg aagtcgaggc atttctgtcc tggctggaac accccttgta ttactgttta
     6781 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     6841 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     6901 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
     6961 tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc
     7021 tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag cgggcttttt
     7081 tatccccgga agcctgtgga tagagggtag ttatccacgt gaaaccgcta atgccccgca
     7141 aagccttgat tcacggggct ttccggcccg ctccaaaaac tatccacgtg aaatcgctaa
     7201 tcagggtacg tgaaatcgct aatcggagta cgtgaaatcg ctaataaggt cacgtgaaat
     7261 cgctaatcaa aaaggcacgt gagaacgcta atagcccttt cagatcaaca gcttgcaaac
     7321 acccctcgct ccggcaagta gttacagcaa gtagtatgtt caattagctt ttcaattatg
     7381 aatatatata tcaattattg gtcgcccttg gcttgtggac aatgcgctac gcgcaccggc
     7441 tccgcccgtg gacaaccgca agcggttgcc caccgtcgag cgcctttgcc cacaacccgg
     7501 cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa aagcccgaaa
     7561 ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt ggcaggatat
     7621 attgtggtg
//
