LOCUS       Exported               10800 bp ds-DNA     circular SYN 30-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Princeton Network
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    550..729
                     /gene="cPPT"
                     /label=cPPT
                     /note="/apeinfo_fwdcolor=pink"
                     /note="/apeinfo_revcolor=pink"
                     /note="/vntifkey=34"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="/apeinfo_fwdcolor=pink"
                     /note="/apeinfo_revcolor=pink"
                     /note="/vntifkey=27"
     promoter        762..1255
                     /gene="pSFFV"
                     /label=SFFV
                     /note="PROMOTER "
                     /note="/apeinfo_fwdcolor=#797884"
                     /note="/apeinfo_revcolor=#797884"
                     /note="/vntifkey=29"
     misc_feature    1180..1203
                     /gene="*"
                     /label=1166_seq
                     /note="/apeinfo_fwdcolor=pink"
                     /note="/apeinfo_revcolor=pink"
                     /note="/vntifkey=27"
     primer_bind     complement(1289..1320)
                     /label=Grb2-back-R
     primer_bind     1306..1338
                     /label=DNcSRC-F
     CDS             1321..1566
                     /codon_start=1
                     
                     /translation="MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADG
                     HRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLA"
     CDS             1567..2430
                     /codon_start=1
                     
                     /translation="PTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTT
                     RVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDF
                     LKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLA
                     RLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMV
                     NREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQ
                     YQPGENL"
     primer_bind     complement(2393..2428)
                     /label=SRC-Y529-R
     primer_bind     2396..2435
                     /label=SRC-Y529-F
     primer_bind     complement(2418..2445)
                     /label=SRC-R
     primer_bind     2431..2457
                     /label=SRC-back-F
     CDS             2446..3144
                     /codon_start=1
                     /gene="TagBFP"
                     /label=TagBFP
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(2490..2508)
                     /label=BFP-StartOut-R
     misc_feature    3232..3254
                     /gene="**"
                     /label=1167_seq
                     /note="/apeinfo_fwdcolor=pink"
                     /note="/apeinfo_revcolor=pink"
                     /note="/vntifkey=27"
     misc_feature    3241..3832
                     /gene="wPRE"
                     /label=WPRE
                     /note="/apeinfo_fwdcolor=pink"
                     /note="/apeinfo_revcolor=pink"
                     /note="/vntifkey=9"
     gene            5063..5923
                     /gene="AmpR"
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="CDS "
                     /note="/apeinfo_fwdcolor=#9e9100"
                     /note="/apeinfo_revcolor=#9e9100"
                     /note="/vntifkey=4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
      781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta
      841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 atgggcagca acaagagcaa gcccaaggac gccagccagc ggcgccgcag cctggagccc
     1381 tcggaaaacg tgcacggggc agggggcgcc ttcccggcct cacagacacc gagcaagccc
     1441 gcctccgccg acggccaccg cgggcccagc gccgccttcg tgccgcccgc ggccgagccc
     1501 aagctcttcg gaggcttcaa ctcctcggac accgtcacct ccccgcagag ggcggggcct
     1561 ctggcaccca catccaagcc tcagacccag ggattggcca aggatgcgtg ggagatcccc
     1621 cgggagtccc tgcggctgga ggtcaagctg ggccagggtt gcttcggaga ggtgtggatg
     1681 gggacctgga acggcaccac gagggttgcc atcaagactc tgaagccagg caccatgtcc
     1741 ccagaggcct tcctgcagga ggcccaagtc atgaagaaac tgaggcacga gaaactggtg
     1801 cagctgtatg ctgtggtgtc ggaagaaccc atttacattg tgacagagta catgaacaag
     1861 gggagtctgc tggactttct caagggggaa acgggcaaat atttgcggct accccagctg
     1921 gtggacatgt ctgctcagat cgcttcaggc atggcctatg tggagcggat gaactatgtg
     1981 caccgggacc ttcgagccgc caatatccta gtaggggaga acctggtgtg caaagtggcc
     2041 gactttgggt tggcccggct catagaagac aacgaataca cagcccggca aggtgccaaa
     2101 ttccccatca agtggaccgc ccctgaagct gctctgtacg gcaggttcac catcaagtcg
     2161 gatgtgtggt cctttgggat tctgctgacc gagctcacca ctaagggaag agtgccctat
     2221 cctgggatgg tgaaccgtga ggttctggac caggtggagc ggggctaccg gatgccttgt
     2281 ccccccgagt gccccgagtc cctgcatgac cttatgtgcc agtgctggcg gaaggagccc
     2341 gaggagcggc ccaccttcga gtacctgcag gccttcctgg aagactactt tacgtccact
     2401 gagccacagt accagcccgg ggagaaccta ggatccggag ctaccatgag cgagctgatt
     2461 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
     2521 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     2581 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
     2641 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
     2701 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     2761 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     2821 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
     2881 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
     2941 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag
     3001 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
     3061 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
     3121 agcaaactgg ggcacaagct taatgcggcc gcaactccca cctgcaacat gcgtgactga
     3181 ctgaggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     3241 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3301 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3361 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3421 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3481 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3541 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3601 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3661 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3721 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3781 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3841 tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3901 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3961 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4021 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4081 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     4141 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     4201 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     4261 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     4321 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     4381 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     4441 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     4501 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     4561 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     4621 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     4681 tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4861 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4921 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4981 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5041 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5101 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5161 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5221 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5281 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5341 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5401 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5461 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5521 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5581 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5641 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5701 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5761 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5821 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5881 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5941 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6001 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6061 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6121 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6181 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6241 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6301 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6361 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6421 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6481 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6541 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6601 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6661 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6721 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6781 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6841 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6901 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7081 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7141 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7261 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     7321 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     7381 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     7441 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     7501 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     7561 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     7621 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     7681 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     7741 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     7801 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
     7861 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     7921 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     7981 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     8041 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     8101 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     8161 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
     8221 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     8281 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
     8341 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     8401 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     8461 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     8521 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     8581 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     8641 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     8701 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     8761 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     8821 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     8881 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     8941 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     9001 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     9061 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9121 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9181 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
     9241 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9301 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     9361 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     9421 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9481 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9541 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9601 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9661 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     9721 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     9781 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9961 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10021 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10081 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10141 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10201 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10261 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10321 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10381 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10441 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt cagacctgga
    10501 cgatatatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10561 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10621 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10681 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10741 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
//
