LOCUS       Exported                7257 bp ds-DNA     circular SYN 26-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Aleks Penev
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    48..203
                     /label=SV40 promoter
                     /note="color: #f58a5e"
     misc_feature    184
                     /label=Probable major TSS
                     /note="color: #faac61"
     promoter        453..471
                     /label=tac promoter
                     /note="color: #75c6a9"
     primer_bind     476..495
                     /label=Renilla-F
                     /note="color: black; sequence: GCTTGGCATTCCGGTACTGA"
     misc_feature    519..1454
                     /label=Renilla luciferase
                     /note="color: #ffef86"
     primer_bind     1400..1421
                     /label=hpc F
                     /note="color: black; sequence: aatgggaaaatatatcaaatcg"
     primer_bind     complement(1469..1489)
                     /label=renilla-R
                     /note="color: black; sequence: TGATTCGAGGTCGACGGTATC"
     stem_loop       1507..1586
                     /label=Strong Hairpin
                     /note="color: #ff0000"
     primer_bind     1598..1634
                     /label=Primer 13
                     /note="color: black; sequence: 
                     aaaaaaCCATGGAAGTCCTCAGCCTTCACGTCCGGCATTCGT"
     misc_feature    1690..2277
                     /label=TpC
                     /note="color: #99cc00"
     primer_bind     complement(1704..1721)
                     /label=TpC R
                     /note="color: black; sequence: CGTGCGGCGACCCTTTGG"
     misc_feature    1958
                     /label=-245
                     /note="color: #a6acb3"
     misc_feature    2012..2277
                     /label=TERT
                     /note="color: #a6acb3"
     misc_feature    2057
                     /label=-146
                     /note="color: #a6acb3"
     primer_bind     2068..2090
                     /label=Primer 2
                     /note="color: black; sequence: ggcccagccccttccgggccctc"
     primer_bind     complement(2068..2090)
                     /label=Primer 1
                     /note="color: black; sequence: gagggcccggaaggggctgggcc"
     misc_feature    2079
                     /label=-124
                     /note="color: #a6acb3"
     misc_feature    2146
                     /label=-57
                     /note="color: #a6acb3"
     primer_bind     complement(2259..2284)
                     /label=Primer 12
                     /note="color: black; sequence: 
                     aaaaaaccatgggGGaCAGCGGCAGCACCTCG"
     CDS             2281..3933
                     /label=Firefly luciferase
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     complement(2282..2303)
                     /label=GLprimer2
                     /note="color: black; sequence: TTTATGTTTTTGGCGTCTTCCA"
     polyA_signal    3965..4156
                     /label=SV40 late polyA
                     /note="color: #d59687"
     rep_origin      complement(4742..5424)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     CDS             complement(5522..6181)
                     /label=AmpR
                     /note="color: #ffef86; direction: LEFT"
     rep_origin      6528..6834
                     /label=F1 ori
                     /note="color: #9eafd2"
     CDS             complement(6968..7036)
                     /label=LacZ alpha
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctg aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      301 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      361 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      421 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctaggcttg
      481 gcattccggt actgaattga tatcaagctc tagccaccat gacttcgaaa gtttatgatc
      541 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
      601 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
      661 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
      721 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
      781 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
      841 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
      901 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
      961 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1021 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1081 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1141 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1201 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1261 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     1321 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     1381 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     1441 aaaatgaaca ataattctag agcttatcga taccgtcgac ctcgaatcac tagtcagctg
     1501 gaattcagat ctggtaccga gctccccggg ctgcaggata tcctgcagcc cggggacctc
     1561 ggtaccagat ctaggatcca ggatccggcg acactccacc atggaagtcc tcagccttca
     1621 cgtccggcat tcgtggtgcc cggagcccga cgccccgcgt ccggacctgg aggcagccct
     1681 gggtctccgg atcaggccag cggccaaagg gtcgccgcac gcacctgttc ccagggcctc
     1741 cacatcatgg cccctccctc gggttacccc acagcctagg ccgattcgac ctctctccgc
     1801 tggggccctc gctggcgtcc ctgcaccctg ggagcgcgag cggcgcgcgg gcggggaagc
     1861 gcggcccaga cccccgggtc cgcccggagc agctgcgctg tcggggccag gccgggctcc
     1921 cagtggattc gcgggcacag acgcccagga ccgcgcttcc cacgtggcgg agggactggg
     1981 gacccgggca cccgtcctgc cccttcacct tccagctccg cctcctccgc gcggaccccg
     2041 ccccgtcccg acccctcccg ggtccccggc ccagccccct ccgggccctc ccagcccctc
     2101 cccttccttt ccgcggcccc gccctctcct cgcggcgcga gtttcaggca gcgctgcgtc
     2161 ctgctgcgca cgtgggaagc cctggccccg gccacccccg cgatgccgcg cgctccccgc
     2221 tgccgagccg tgcgctccct gctgcgcagc cactaccgcg aggtgctgcc gctgtccccc
     2281 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
     2341 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     2401 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
     2461 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     2521 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     2581 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     2641 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     2701 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     2761 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     2821 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     2881 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     2941 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     3001 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     3061 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     3121 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     3181 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     3241 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     3301 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     3361 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     3421 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     3481 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     3541 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     3601 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     3661 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     3721 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     3781 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     3841 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     3901 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     3961 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4021 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4081 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4141 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     4201 ctgaacgatg gagcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     4261 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4321 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     4381 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     4441 gcggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     4501 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     4561 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6481 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     6541 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6601 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6661 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6721 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6781 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6841 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6901 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6961 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     7021 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg
     7081 tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     7141 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     7201 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata
//
