LOCUS       Exported                7817 bp ds-DNA     circular SYN 07-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Aleks Penev
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    48..203
                     /label=SV40 promoter
                     /note="color: #f58a5e"
     misc_feature    184
                     /label=Probable major TSS
                     /note="color: #faac61"
     promoter        453..471
                     /label=tac promoter
                     /note="color: #75c6a9"
     misc_feature    519..1454
                     /label=Renilla luciferase
                     /note="color: #ffef86"
     primer_bind     1400..1421
                     /label=hpc F
                     /note="color: black; sequence: aatgggaaaatatatcaaatcg"
     stem_loop       1507..1586
                     /label=Strong Hairpin
                     /note="color: #ff0000"
     primer_bind     1609..1634
                     /label=Primer 1
                     /note="color: black; sequence: 
                     aaaaaacctcaggCTGGCACTGCTGCCTGTGA"
     primer_bind     complement(2146..2174)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     aaaaaacctgaggGACCCAGAGGCAGTAAGAGAACG"
     primer_bind     2169..2194
                     /label=Primer 13
                     /note="color: black; sequence: 
                     aaaaaaCCATGGAAGTCCTCAGCCTTCACGTCCGGCATTCGT"
     misc_feature    2250..2837
                     /label=TpC
                     /note="color: #99cc00"
     primer_bind     complement(2264..2281)
                     /label=TpC R
                     /note="color: black; sequence: CGTGCGGCGACCCTTTGG"
     misc_feature    2518
                     /label=-245
                     /note="color: #a6acb3"
     misc_feature    2572..2837
                     /label=TERT
                     /note="color: #a6acb3"
     primer_bind     complement(2603..2625)
                     /label=Primer 3
                     /note="color: black; sequence: ggacccggaaggggtcgggacgg"
     primer_bind     2603..2625
                     /label=Primer 4
                     /note="color: black; sequence: ccgtcccgaccccttccgggtcc"
     misc_feature    2617
                     /label=-146
                     /note="color: #a6acb3"
     primer_bind     complement(2628..2650)
                     /label=Primer 1
                     /note="color: black; sequence: gagggcccggaaggggctgggcc"
     primer_bind     2628..2650
                     /label=Primer 2
                     /note="color: black; sequence: ggcccagccccttccgggccctc"
     misc_feature    2639
                     /label=-124
                     /note="color: #a6acb3"
     primer_bind     complement(2693..2716)
                     /label=Primer 5
                     /note="color: black; sequence: cagcgctgccggaaactcgcgccg"
     primer_bind     2693..2716
                     /label=Primer 6
                     /note="color: black; sequence: cggcgcgagtttccggcagcgctg"
     misc_feature    2706
                     /label=-57
                     /note="color: #a6acb3"
     primer_bind     complement(2819..2844)
                     /label=Primer 12
                     /note="color: black; sequence: 
                     aaaaaaccatgggGGaCAGCGGCAGCACCTCG"
     CDS             2841..4493
                     /label=Firefly luciferase
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     complement(2842..2863)
                     /label=GLprimer2
                     /note="color: black; sequence: TTTATGTTTTTGGCGTCTTCCA"
     polyA_signal    4525..4716
                     /label=SV40 late polyA
                     /note="color: #d59687"
     rep_origin      complement(5302..5984)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     CDS             complement(6082..6741)
                     /label=AmpR
                     /note="color: #ffef86; direction: LEFT"
     rep_origin      7088..7394
                     /label=F1 ori
                     /note="color: #9eafd2"
     CDS             complement(7528..7596)
                     /label=LacZ alpha
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctg aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      301 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      361 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      421 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctaggcttg
      481 gcattccggt actgaattga tatcaagctc tagccaccat gacttcgaaa gtttatgatc
      541 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
      601 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
      661 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
      721 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
      781 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
      841 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
      901 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
      961 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1021 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1081 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1141 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1201 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1261 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     1321 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     1381 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     1441 aaaatgaaca ataattctag agcttatcga taccgtcgac ctcgaatcac tagtcagctg
     1501 gaattcagat ctggtaccga gctccccggg ctgcaggata tcctgcagcc cggggacctc
     1561 ggtaccagat ctaggatcca ggatccggcg acactccacc atggaagtcc tcaggctggc
     1621 actgctgcct gtgagtgagg acagtgatgc cagccagagg acaaatggga tgcacagggc
     1681 gtccctgtgc cccccaaggg aatgaaaaag gggtctgagg gctctctagc aggagaggag
     1741 gggcgcccca caccgcccag gtcctagaac ccaggtggaa ggaggaggcc tgtggactga
     1801 ggggaggaag gaacagacag gggcctgcgg acagacaggt ggaccccgac aggcccagat
     1861 aggaagcgcg ttgtggcatt tctgccgcag gacccctttc ccttgcccgc tagagggcgg
     1921 cgctgcccca gagacgcaca gccagcggct tagccaaggc cccagcacca gcggccctgg
     1981 gccctgccct gaccctgtgc tgactctgca gcagcctcag ctccgagtcc tagtgtctgt
     2041 cgtgtgaatt atgatttgtg accaaaacct ttgtctttca tgtgagttat ctgtgactga
     2101 aagccttctt tctctctccc ctgagttacc atttatgact aaaaccgttc tcttactgcc
     2161 tctgggtccc tcagccttca cgtccggcat tcgtggtgcc cggagcccga cgccccgcgt
     2221 ccggacctgg aggcagccct gggtctccgg atcaggccag cggccaaagg gtcgccgcac
     2281 gcacctgttc ccagggcctc cacatcatgg cccctccctc gggttacccc acagcctagg
     2341 ccgattcgac ctctctccgc tggggccctc gctggcgtcc ctgcaccctg ggagcgcgag
     2401 cggcgcgcgg gcggggaagc gcggcccaga cccccgggtc cgcccggagc agctgcgctg
     2461 tcggggccag gccgggctcc cagtggattc gcgggcacag acgcccagga ccgcgcttcc
     2521 cacgtggcgg agggactggg gacccgggca cccgtcctgc cccttcacct tccagctccg
     2581 cctcctccgc gcggaccccg ccccgtcccg acccctcccg ggtccccggc ccagccccct
     2641 ccgggccctc ccagcccctc cccttccttt ccgcggcccc gccctctcct cgcggcgcga
     2701 gtttcaggca gcgctgcgtc ctgctgcgca cgtgggaagc cctggccccg gccacccccg
     2761 cgatgccgcg cgctccccgc tgccgagccg tgcgctccct gctgcgcagc cactaccgcg
     2821 aggtgctgcc gctgtccccc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
     2881 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
     2941 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
     3001 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
     3061 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
     3121 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     3181 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
     3241 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
     3301 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     3361 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
     3421 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
     3481 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     3541 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     3601 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
     3661 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     3721 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     3781 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     3841 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     3901 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     3961 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     4021 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     4081 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     4141 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     4201 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     4261 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     4321 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     4381 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     4441 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga
     4501 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     4561 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4621 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4681 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4741 gtggtaaaat cgataaggat ctgaacgatg gagcggagaa tgggcggaac tgggcggagt
     4801 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     4861 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     4921 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     4981 taactgacac acattccaca gcggatccgt cgaccgatgc ccttgagagc cttcaaccca
     5041 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     5101 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     5161 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5221 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5281 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5341 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5401 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5461 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5521 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5581 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5641 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5701 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5761 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5821 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5881 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5941 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6001 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6061 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6121 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6181 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6241 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6301 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6361 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6421 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6481 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6541 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6601 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6661 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6721 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6781 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6841 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6901 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6961 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7021 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     7081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7141 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7261 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7381 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7441 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7501 taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga
     7561 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
     7621 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
     7681 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     7741 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     7801 acatttctct atcgata
//
