LOCUS       Exported                7841 bp ds-DNA     circular SYN 06-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Aleks Penev
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    48..203
                     /label=SV40 promoter
                     /note="color: #f58a5e"
     misc_feature    184
                     /label=Probable major TSS
                     /note="color: #faac61"
     promoter        453..471
                     /label=tac promoter
                     /note="color: #75c6a9"
     misc_feature    519..1454
                     /label=Renilla luciferase
                     /note="color: #ffef86"
     primer_bind     1400..1421
                     /label=hpc F
                     /note="color: black; sequence: aatgggaaaatatatcaaatcg"
     stem_loop       1507..1586
                     /label=Strong Hairpin
                     /note="color: #ff0000"
     primer_bind     1609..1635
                     /label=Primer 1
                     /note="color: black; sequence: 
                     aaaaaacctcaggGCACAGGTGGGTCTGTCCTG"
     primer_bind     complement(2172..2198)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     aaaaaacctgaggTGCACTGGTTAGAAGGCTCCA"
     primer_bind     2193..2218
                     /label=Primer 13
                     /note="color: black; sequence: 
                     aaaaaaCCATGGAAGTCCTCAGCCTTCACGTCCGGCATTCGT"
     misc_feature    2274..2861
                     /label=TpC
                     /note="color: #99cc00"
     primer_bind     complement(2288..2305)
                     /label=TpC R
                     /note="color: black; sequence: CGTGCGGCGACCCTTTGG"
     misc_feature    2542
                     /label=-245
                     /note="color: #a6acb3"
     misc_feature    2596..2861
                     /label=TERT
                     /note="color: #a6acb3"
     primer_bind     complement(2627..2649)
                     /label=Primer 3
                     /note="color: black; sequence: ggacccggaaggggtcgggacgg"
     primer_bind     2627..2649
                     /label=Primer 4
                     /note="color: black; sequence: ccgtcccgaccccttccgggtcc"
     misc_feature    2641
                     /label=-146
                     /note="color: #a6acb3"
     primer_bind     complement(2652..2674)
                     /label=Primer 1
                     /note="color: black; sequence: gagggcccggaaggggctgggcc"
     primer_bind     2652..2674
                     /label=Primer 2
                     /note="color: black; sequence: ggcccagccccttccgggccctc"
     misc_feature    2663
                     /label=-124
                     /note="color: #a6acb3"
     primer_bind     complement(2717..2740)
                     /label=Primer 5
                     /note="color: black; sequence: cagcgctgccggaaactcgcgccg"
     primer_bind     2717..2740
                     /label=Primer 6
                     /note="color: black; sequence: cggcgcgagtttccggcagcgctg"
     misc_feature    2730
                     /label=-57
                     /note="color: #a6acb3"
     primer_bind     complement(2843..2868)
                     /label=Primer 12
                     /note="color: black; sequence: 
                     aaaaaaccatgggGGaCAGCGGCAGCACCTCG"
     CDS             2865..4517
                     /label=Firefly luciferase
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     complement(2866..2887)
                     /label=GLprimer2
                     /note="color: black; sequence: TTTATGTTTTTGGCGTCTTCCA"
     polyA_signal    4549..4740
                     /label=SV40 late polyA
                     /note="color: #d59687"
     rep_origin      complement(5326..6008)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     CDS             complement(6106..6765)
                     /label=AmpR
                     /note="color: #ffef86; direction: LEFT"
     rep_origin      7112..7418
                     /label=F1 ori
                     /note="color: #9eafd2"
     CDS             complement(7552..7620)
                     /label=LacZ alpha
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctg aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      301 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      361 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      421 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctaggcttg
      481 gcattccggt actgaattga tatcaagctc tagccaccat gacttcgaaa gtttatgatc
      541 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
      601 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
      661 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
      721 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
      781 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
      841 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
      901 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
      961 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1021 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1081 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1141 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1201 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1261 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     1321 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     1381 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     1441 aaaatgaaca ataattctag agcttatcga taccgtcgac ctcgaatcac tagtcagctg
     1501 gaattcagat ctggtaccga gctccccggg ctgcaggata tcctgcagcc cggggacctc
     1561 ggtaccagat ctaggatcca ggatccggcg acactccacc atggaagtcc tcagggcaca
     1621 ggtgggtctg tcctgcccat gtggggcccg cattgccctc ccaccccggc ccttccagaa
     1681 ccctcttgcc cagggaggga gcgtaggggg cagaccctct acgtgtgaca tgcaatcagc
     1741 tcacggcgat tcacacgtgg tcctggggcc tgaacggctt ggtcaccacc ggcttctggt
     1801 ccgaccagca cagcggggcc agcggggctt ccctgcatgc tgctgctctg tcggggccgg
     1861 caagatggcc cctggggttg gaataagaca ggtcctccca cgatacgtgc aaccctcact
     1921 gctcgctggg actgtcgggg ccctttcctg cctccggggc tgtggggtcc tctggaatta
     1981 gaccacttcc cctttagaaa ggtctcagga tggttccaca agcccagaga ggcccacagt
     2041 ggtgacagtg tgtggagtgg gcagttgcag gcaagggccc cagcccggga gcgcaaacac
     2101 tctgaggctg ccagacttgc acagggaaga gggagcagcg tgaggcccag ggtgcacctg
     2161 actgttgggg ctggagcctt ctaaccagtg cacctcagcc ttcacgtccg gcattcgtgg
     2221 tgcccggagc ccgacgcccc gcgtccggac ctggaggcag ccctgggtct ccggatcagg
     2281 ccagcggcca aagggtcgcc gcacgcacct gttcccaggg cctccacatc atggcccctc
     2341 cctcgggtta ccccacagcc taggccgatt cgacctctct ccgctggggc cctcgctggc
     2401 gtccctgcac cctgggagcg cgagcggcgc gcgggcgggg aagcgcggcc cagacccccg
     2461 ggtccgcccg gagcagctgc gctgtcgggg ccaggccggg ctcccagtgg attcgcgggc
     2521 acagacgccc aggaccgcgc ttcccacgtg gcggagggac tggggacccg ggcacccgtc
     2581 ctgccccttc accttccagc tccgcctcct ccgcgcggac cccgccccgt cccgacccct
     2641 cccgggtccc cggcccagcc ccctccgggc cctcccagcc cctccccttc ctttccgcgg
     2701 ccccgccctc tcctcgcggc gcgagtttca ggcagcgctg cgtcctgctg cgcacgtggg
     2761 aagccctggc cccggccacc cccgcgatgc cgcgcgctcc ccgctgccga gccgtgcgct
     2821 ccctgctgcg cagccactac cgcgaggtgc tgccgctgtc ccccatggaa gacgccaaaa
     2881 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
     2941 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3001 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
     3061 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3121 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3181 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
     3241 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
     3301 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3361 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
     3421 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
     3481 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
     3541 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     3601 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     3661 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     3721 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     3781 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     3841 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     3901 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     3961 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4021 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     4081 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4141 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     4201 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     4261 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4321 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4381 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     4441 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     4501 gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     4561 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4621 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4681 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4741 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatctgaac gatggagcgg
     4801 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     4861 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     4921 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     4981 ggagcctggg gactttccac accctaactg acacacattc cacagcggat ccgtcgaccg
     5041 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     5101 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     5161 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5221 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5761 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7081 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc
     7561 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     7621 gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc
     7681 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     7741 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     7801 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
