LOCUS       Exported                7874 bp ds-DNA     circular SYN 30-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Aleks Penev
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    48..203
                     /label=SV40 promoter
                     /note="color: #f58a5e"
     misc_feature    184
                     /label=Probable major TSS
                     /note="color: #faac61"
     promoter        453..471
                     /label=tac promoter
                     /note="color: #75c6a9"
     misc_feature    519..1454
                     /label=Renilla luciferase
                     /note="color: #ffef86"
     primer_bind     1400..1421
                     /label=hpc F
                     /note="color: black; sequence: aatgggaaaatatatcaaatcg"
     stem_loop       1507..1586
                     /label=Strong Hairpin
                     /note="color: #ff0000"
     primer_bind     1609..1643
                     /label=Primer 1
                     /note="color: black; sequence: 
                     aaaaaacctcagggctcgagcCCACCTCCAGCCAAAACAGG"
     primer_bind     1609..1643
                     /label=Primer 8
                     /note="color: black; sequence: 
                     aaaaaacctcagggctcgagcCCACCTCCAGCCAAAACAGG"
     primer_bind     complement(2198..2231)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     primer_bind     complement(2198..2231)
                     /label=Primer 9
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     primer_bind     complement(2198..2231)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     primer_bind     2223..2251
                     /label=Primer 13
                     /note="color: black; sequence: 
                     aaaaaaCCATGGAAGTCCTCAGCCTTCACGTCCGGCATTCGT"
     misc_feature    2307..2894
                     /label=TpC
                     /note="color: #99cc00"
     primer_bind     complement(2321..2338)
                     /label=TpC R
                     /note="color: black; sequence: CGTGCGGCGACCCTTTGG"
     misc_feature    2575
                     /label=-245
                     /note="color: #a6acb3"
     misc_feature    2629..2894
                     /label=TERT
                     /note="color: #a6acb3"
     primer_bind     complement(2660..2682)
                     /label=Primer 3
                     /note="color: black; sequence: ggacccggaaggggtcgggacgg"
     primer_bind     2660..2682
                     /label=Primer 4
                     /note="color: black; sequence: ccgtcccgaccccttccgggtcc"
     misc_feature    2674
                     /label=-146
                     /note="color: #a6acb3"
     primer_bind     complement(2685..2707)
                     /label=Primer 1
                     /note="color: black; sequence: gagggcccggaaggggctgggcc"
     primer_bind     2685..2707
                     /label=Primer 2
                     /note="color: black; sequence: ggcccagccccttccgggccctc"
     misc_feature    2696
                     /label=-124
                     /note="color: #a6acb3"
     primer_bind     complement(2750..2773)
                     /label=Primer 5
                     /note="color: black; sequence: cagcgctgccggaaactcgcgccg"
     primer_bind     2750..2773
                     /label=Primer 6
                     /note="color: black; sequence: cggcgcgagtttccggcagcgctg"
     misc_feature    2763
                     /label=-57
                     /note="color: #a6acb3"
     primer_bind     complement(2876..2901)
                     /label=Primer 12
                     /note="color: black; sequence: 
                     aaaaaaccatgggGGaCAGCGGCAGCACCTCG"
     CDS             2898..4550
                     /label=Firefly luciferase
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     complement(2899..2920)
                     /label=GLprimer2
                     /note="color: black; sequence: TTTATGTTTTTGGCGTCTTCCA"
     polyA_signal    4582..4773
                     /label=SV40 late polyA
                     /note="color: #d59687"
     rep_origin      complement(5359..6041)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     CDS             complement(6139..6798)
                     /label=AmpR
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     6329..6360
                     /label=Primer 2
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     primer_bind     6329..6360
                     /label=Primer 9
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     primer_bind     6329..6360
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aaaaaacctgagggctcgagcCTGTGGGCTCCTGCAGCTTT"
     rep_origin      7145..7451
                     /label=F1 ori
                     /note="color: #9eafd2"
     CDS             complement(7585..7653)
                     /label=LacZ alpha
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctg aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      301 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      361 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      421 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctaggcttg
      481 gcattccggt actgaattga tatcaagctc tagccaccat gacttcgaaa gtttatgatc
      541 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
      601 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
      661 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
      721 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
      781 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
      841 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
      901 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
      961 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1021 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1081 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1141 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1201 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1261 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     1321 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     1381 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     1441 aaaatgaaca ataattctag agcttatcga taccgtcgac ctcgaatcac tagtcagctg
     1501 gaattcagat ctggtaccga gctccccggg ctgcaggata tcctgcagcc cggggacctc
     1561 ggtaccagat ctaggatcca ggatccggcg acactccacc atggaagtcc tcagggctcg
     1621 agcccacctc cagccaaaac aggaccttca cacataccag cacgcttccc attcagccca
     1681 ggccctgaga caggccctcc ggagctgtgg cccagggaaa cgccaatctc cccggacctc
     1741 ccgcctggtc tgagtaaaaa gaatcctgaa attcaggatc cgtggcattt ctagctgctt
     1801 gcatttgttt aacaaacatc tgcagaaagc cagtggggcc ctggtggaga gcacctccca
     1861 agccccctgt ggtgggagcc ccacccccag cacatcctgc atcaggggat cctgctacaa
     1921 gaaagagtcc ccagcctgct ggcccttcct actccgaact ggccgcagat acagggagcc
     1981 ccccagcaca tcctgcatca ggggatcctg ctacaagaaa gagtccccag cctgccggcc
     2041 cttcctactc cgaactggcc gcagatacag ggagcccccc agcacatcct gcatcagggg
     2101 atcctgctac aagagtcccc agcctgcccg gcccttccta ctccgaactg gctgcagata
     2161 cagggagccc cccagcacat cctggcccaa accctcaaaa gctgcaggag cccacaggct
     2221 cgagccctca gccttcacgt ccggcattcg tggtgcccgg agcccgacgc cccgcgtccg
     2281 gacctggagg cagccctggg tctccggatc aggccagcgg ccaaagggtc gccgcacgca
     2341 cctgttccca gggcctccac atcatggccc ctccctcggg ttaccccaca gcctaggccg
     2401 attcgacctc tctccgctgg ggccctcgct ggcgtccctg caccctggga gcgcgagcgg
     2461 cgcgcgggcg gggaagcgcg gcccagaccc ccgggtccgc ccggagcagc tgcgctgtcg
     2521 gggccaggcc gggctcccag tggattcgcg ggcacagacg cccaggaccg cgcttcccac
     2581 gtggcggagg gactggggac ccgggcaccc gtcctgcccc ttcaccttcc agctccgcct
     2641 cctccgcgcg gaccccgccc cgtcccgacc cctcccgggt ccccggccca gccccctccg
     2701 ggccctccca gcccctcccc ttcctttccg cggccccgcc ctctcctcgc ggcgcgagtt
     2761 tcaggcagcg ctgcgtcctg ctgcgcacgt gggaagccct ggccccggcc acccccgcga
     2821 tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac taccgcgagg
     2881 tgctgccgct gtcccccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct
     2941 atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc
     3001 tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg
     3061 agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa
     3121 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg
     3181 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc
     3241 tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa
     3301 aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta
     3361 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg
     3421 gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga
     3481 tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg
     3541 cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata
     3601 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat
     3661 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga
     3721 ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct
     3781 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg
     3841 gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag
     3901 gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg
     3961 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg
     4021 atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc
     4081 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg
     4141 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg
     4201 ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg
     4261 aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg
     4321 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg
     4381 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag
     4441 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
     4501 tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc
     4561 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     4621 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4681 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4741 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4801 gtaaaatcga taaggatctg aacgatggag cggagaatgg gcggaactgg gcggagttag
     4861 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     4921 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     4981 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     5041 ctgacacaca ttccacagcg gatccgtcga ccgatgccct tgagagcctt caacccagtc
     5101 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     5161 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     5221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     7141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7561 caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg
     7621 gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa
     7681 tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat
     7741 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     7801 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     7861 tttctctatc gata
//
