LOCUS       Exported                7852 bp ds-DNA     circular SYN 01-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Aleks Penev
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, May 1, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    48..203
                     /label=SV40 promoter
     misc_feature    184
                     /label=Probable major TSS
     promoter        453..471
                     /label=tac promoter
     misc_feature    519..1454
                     /label=Renilla luciferase
     primer_bind     1400..1421
                     /label=hpc F
     stem_loop       1507..1586
                     /label=Strong Hairpin
     primer_bind     1609..1635
                     /label=Primer 1
     primer_bind     1609..1635
                     /label=Primer 10
     primer_bind     complement(2182..2209)
                     /label=Primer 2
     primer_bind     2204..2229
                     /label=Primer 13
     misc_feature    2285..2872
                     /label=TpC
     primer_bind     complement(2299..2316)
                     /label=TpC R
     misc_feature    2553
                     /label=-245
     misc_feature    2607..2872
                     /label=TERT
     primer_bind     complement(2638..2660)
                     /label=Primer 3
     primer_bind     2638..2660
                     /label=Primer 4
     misc_feature    2652
                     /label=-146
     primer_bind     complement(2663..2685)
                     /label=Primer 1
     primer_bind     2663..2685
                     /label=Primer 2
     misc_feature    2674
                     /label=-124
     primer_bind     complement(2728..2751)
                     /label=Primer 5
     primer_bind     2728..2751
                     /label=Primer 6
     misc_feature    2741
                     /label=-57
     primer_bind     complement(2854..2879)
                     /label=Primer 12
     CDS             2876..4528
                     /label=Firefly luciferase
     primer_bind     complement(2877..2898)
                     /label=GLprimer2
     polyA_signal    4560..4751
                     /label=SV40 late polyA
     rep_origin      complement(5337..6019)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6117..6776)
                     /label=AmpR
     rep_origin      7123..7429
                     /label=F1 ori
     CDS             complement(7563..7631)
                     /label=LacZ alpha
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctg aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      301 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      361 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      421 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctaggcttg
      481 gcattccggt actgaattga tatcaagctc tagccaccat gacttcgaaa gtttatgatc
      541 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
      601 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
      661 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
      721 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
      781 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
      841 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
      901 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
      961 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1021 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1081 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1141 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1201 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1261 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     1321 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     1381 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     1441 aaaatgaaca ataattctag agcttatcga taccgtcgac ctcgaatcac tagtcagctg
     1501 gaattcagat ctggtaccga gctccccggg ctgcaggata tcctgcagcc cggggacctc
     1561 ggtaccagat ctaggatcca ggatccggcg acactccacc atggaagtcc tcaggggccc
     1621 tggcaagtat gtccactcct ctgagtgcag ggaggtacag aggtgaccag cctgacgtgc
     1681 agctgtggac acagagggag ggctgctggg ggctggaggc cactgcccag tggcagttgt
     1741 gggggaggca gaaatgacct gatcgctcta aggtgaggga gaggagagcc cactctttac
     1801 agagacttcc ccaatggcac aggcgccacg tggtcctggg gccactgact gggcagacag
     1861 cctggcacag ggacaggaca ggtctagcct gggatgtgct gagcttgatg gatgcatggg
     1921 acccaggagg atgtcacaat gatgattcac atggcacaca ggtgaccatg actcttccct
     1981 gatggagaca atgactcact gctgcagaaa ggtggatcta acccctccag ggtagcctag
     2041 gagaacaaac agactccact ctaaggagcc caggcattcc atgctgatcc acgcctgccc
     2101 agcccggcgg catccccact gtctgtgcat ggacaccagc cctccctggc tccagggccc
     2161 ttgccctcca gtaccttggc ccagctttgg ttctacccca aggcctcagc cttcacgtcc
     2221 ggcattcgtg gtgcccggag cccgacgccc cgcgtccgga cctggaggca gccctgggtc
     2281 tccggatcag gccagcggcc aaagggtcgc cgcacgcacc tgttcccagg gcctccacat
     2341 catggcccct ccctcgggtt accccacagc ctaggccgat tcgacctctc tccgctgggg
     2401 ccctcgctgg cgtccctgca ccctgggagc gcgagcggcg cgcgggcggg gaagcgcggc
     2461 ccagaccccc gggtccgccc ggagcagctg cgctgtcggg gccaggccgg gctcccagtg
     2521 gattcgcggg cacagacgcc caggaccgcg cttcccacgt ggcggaggga ctggggaccc
     2581 gggcacccgt cctgcccctt caccttccag ctccgcctcc tccgcgcgga ccccgccccg
     2641 tcccgacccc tcccgggtcc ccggcccagc cccctccggg ccctcccagc ccctcccctt
     2701 cctttccgcg gccccgccct ctcctcgcgg cgcgagtttc aggcagcgct gcgtcctgct
     2761 gcgcacgtgg gaagccctgg ccccggccac ccccgcgatg ccgcgcgctc cccgctgccg
     2821 agccgtgcgc tccctgctgc gcagccacta ccgcgaggtg ctgccgctgt cccccatgga
     2881 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
     2941 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     3001 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
     3061 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     3121 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     3181 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     3241 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     3301 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     3361 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     3421 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     3481 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     3541 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     3601 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     3661 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     3721 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     3781 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     3841 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     3901 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     3961 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     4021 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     4081 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     4141 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     4201 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     4261 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     4321 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     4381 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     4441 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     4501 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
     4561 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4621 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4681 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4741 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatctgaa
     4801 cgatggagcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4861 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4921 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     4981 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagcgga
     5041 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     5101 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     5161 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5221 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5281 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5341 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5401 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5461 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5521 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5581 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5641 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5701 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5761 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5821 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5881 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5941 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6001 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6061 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6121 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6181 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6241 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6301 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6361 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6421 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6481 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6541 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6601 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6661 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6721 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6781 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6841 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6901 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6961 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7021 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7081 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7141 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7201 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7261 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7321 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7381 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7441 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7501 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7561 tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7621 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt
     7681 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     7741 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     7801 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta
//
