LOCUS       Exported               10496 bp ds-DNA     circular SYN 04-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     61..78
                     /label=5' CAG for
     misc_feature    84..371
                     /label=CAG enhancer
     misc_feature    802..1533
                     /label=B-actin promoter
     primer_bind     807..824
                     /label=5' bActin for
     primer_bind     1477..1493
                     /label=3' bActin for
     primer_bind     1707..1758
                     /label=JRH_gag F
     misc_feature    1719..1724
                     /label=EcorI
     misc_feature    1725..1730
                     /label=Kozak
     CDS             1731..1736
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MG"
     CDS             1737..3230
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="ARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVN
                     PGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKIEE
                     EQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFS
                     PEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIA
                     PGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL
                     DIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGAT
                     LEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEGHI
                     AKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPE
                     ESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     primer_bind     3149..3170
                     /label=Seq Gag 3' F
     primer_bind     complement(3209..3257)
                     /label=JRH_gag cleav R
     CDS             3228..3230
                     /codon_start=1
                     /label=taa removed as next codon
                     /translation="Q"
     CDS             3237..3260
                     /codon_start=1
                     /label=sqnypivq HIV-1 cleavage site
                     /translation="SQNYPIVQ"
     primer_bind     3240..3288
                     /label=JRH_Cleav cas F
     misc_feature    3261..3266
                     /label=AgeI
     CDS             3267..7370
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     7299..7320
                     /label=Cas9 3' seq F
     CDS             7375..7439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="YKDHDGDYKDHDIDYKDDDDK"
     CDS             7458..7478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7485..7505
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7487..7526)
                     /label=JRH_casCag R
     misc_feature    7509..7514
                     /label=NheI
     misc_feature    7583..7639
                     /label=lac operator
     misc_feature    7583..7639
                     /label=beta-globin poly(A) sequence
     primer_bind     7999..8015
                     /label=M13 rev
     primer_bind     complement(7999..8015)
                     /label=M13 rev
                     /note="M13 rev"
     misc_feature    8047..8077
                     /label=lac promoter
     misc_feature    8092..8113
                     /label=CAP binding site
     misc_feature    8172..8367
                     /label=SV40 promoter
     primer_bind     8202..8221
                     /label=SV40 for
     misc_feature    8218..8353
                     /label=SV40 ori
     misc_feature    8373..8507
                     /label=SV40 poly(A)
     misc_feature    8746..9334
                     /label=ori
     misc_feature    9505..10365
                     /label=AMP
     misc_feature    10366..10470
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcgccacc atgggtgcga
     1741 gagcgtcagt attaagcggg ggagaattag atcgatggga aaaaattcgg ttaaggccag
     1801 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1861 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1921 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1981 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     2041 tagaggaaga gcaaaacaaa agtaagaaaa aagcacagca agcagcagct gacacaggac
     2101 acagcaatca ggtcagccaa aattacccta tagtgcagaa catccagggg caaatggtac
     2161 atcaggccat atcacctaga actttaaatg catgggtaaa agtagtagaa gagaaggctt
     2221 tcagcccaga agtgataccc atgttttcag cattatcaga aggagccacc ccacaagatt
     2281 taaacaccat gctaaacaca gtggggggac atcaagcagc catgcaaatg ttaaaagaga
     2341 ccatcaatga ggaagctgca gaatgggata gagtgcatcc agtgcatgca gggcctattg
     2401 caccaggcca gatgagagaa ccaaggggaa gtgacatagc aggaactact agtacccttc
     2461 aggaacaaat aggatggatg acacataatc cacctatccc agtaggagaa atctataaaa
     2521 gatggataat cctgggatta aataaaatag taagaatgta tagccctacc agcattctgg
     2581 acataagaca aggaccaaag gaacccttta gagactatgt agaccgattc tataaaactc
     2641 taagagccga gcaagcttca caagaggtaa aaaattggat gacagaaacc ttgttggtcc
     2701 aaaatgcgaa cccagattgt aagactattt taaaagcatt gggaccagga gcgacactag
     2761 aagaaatgat gacagcatgt cagggagtgg ggggacccgg ccataaagca agagttttgg
     2821 ctgaagcaat gagccaagta acaaatccag ctaccataat gatacagaaa ggcaatttta
     2881 ggaaccaaag aaagactgtt aagtgtttca attgtggcaa agaagggcac atagccaaaa
     2941 attgcagggc ccctaggaaa aagggctgtt ggaaatgtgg aaaggaagga caccaaatga
     3001 aagattgtac tgagagacag gctaattttt tagggaagat ctggccttcc cacaagggaa
     3061 ggccagggaa ttttcttcag agcagaccag agccaacagc cccaccagaa gagagcttca
     3121 ggtttgggga agagacaaca actccctctc agaagcagga gccgatagac aaggaactgt
     3181 atcctttagc ttccctcaga tcactctttg gcagcgaccc ctcgtcacaa aactttagcc
     3241 agaactatcc gattgtgcag accggtatgg acaagaagta cagcatcggc ctggacatcg
     3301 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
     3361 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggcgccctgc
     3421 tgttcgacag cggagaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
     3481 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
     3541 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
     3601 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
     3661 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
     3721 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
     3781 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
     3841 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
     3901 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
     3961 agaagaagaa tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact
     4021 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
     4081 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
     4141 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     4201 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc
     4261 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct
     4321 tcgaccagag caagaacggc tacgccggct acatcgatgg cggagccagc caggaagagt
     4381 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga
     4441 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc
     4501 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat
     4561 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg
     4621 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa
     4681 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgccagcgcc cagagcttca
     4741 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca
     4801 gcctgctgta cgagtacttc accgtgtaca acgagctgac caaagtgaaa tacgtgaccg
     4861 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaagccatc gtggacctgc
     4921 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     4981 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     5041 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     5101 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     5161 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     5221 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     5281 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     5341 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     5401 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     5461 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     5521 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     5581 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     5641 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     5701 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     5761 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
     5821 tcgataacaa agtgctgact cggagcgaca agaaccgggg caagagcgac aacgtgccct
     5881 ccgaagaggt cgtgaagaag atgaagaact actggcgcca gctgctgaat gccaagctga
     5941 ttacccagag gaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     6001 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     6061 cacagatcct ggactcccgg atgaacacta agtacgacga gaacgacaaa ctgatccggg
     6121 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     6181 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     6241 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     6301 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     6361 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     6421 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa acaggcgaga
     6481 tcgtgtggga taagggccgg gactttgcca ccgtgcggaa agtgctgtct atgccccaag
     6541 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     6601 ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     6661 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     6721 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     6781 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     6841 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     6901 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     6961 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     7021 atgagcagaa acagctgttt gtggaacagc acaaacacta cctggacgag atcatcgagc
     7081 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaggtgctga
     7141 gcgcctacaa caagcacaga gacaagccta tcagagagca ggccgagaat atcatccacc
     7201 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     7261 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     7321 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac gccgactata
     7381 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
     7441 gcctgggcag cggctccccc aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga
     7501 aagtgtaagc tagcagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     7561 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     7621 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     7681 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     7741 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     7801 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     7861 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     7921 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc
     7981 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8161 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8221 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8281 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8341 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     8401 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8461 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     8521 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8941 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
