LOCUS       Exported                6844 bp ds-DNA     circular SYN 19-APR-2021
DEFINITION  Bacterial vector for expressing GST fusion proteins with an 
            enterokinase site. For other reading frames, use pET-41b(+) or 
            pET-41c(+).
ACCESSION   .
VERSION     .
KEYWORDS    pET41sIII-GST-ATF6(1-373)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=Terminator_1
                     /note="Terminator_1"
                     /note="T7 terminator color: #ffffff"
     misc_feature    150..173
                     /label=8X His tag
                     /note="8X His tag"
     CDS             complement(186..1304)
                     /codon_start=1
                     /label=ATF6(1-373)
                     /note="ATF6(1-373)"
                     /translation="MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQ
                     LEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSS
                     PRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTEN
                     GLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIIS
                     LQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSP
                     VNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKA
                     ALSENEQLKKENGTLKRQLDEVVSENQRLKVPS"
     misc_feature    1311..1334
                     /label=Precision Protease Cleavage site
                     /note="Precision Protease Cleavage site"
     CDS             complement(1347..2006)
                     /codon_start=1
                     /label=GST tag
                     /note="GST tag"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD"
     RBS             2015..2020
                     /label=RBS_1
                     /note="RBS_1"
                     /note="color: #0000ff"
     protein_bind    2051..2075
                     /label=Protein_Bind_1
                     /note="Protein_Bind_1"
                     /note="lac operator color: #31849b"
     promoter        complement(2076..2094)
                     /label=Promoter_P_1
                     /note="Promoter_P_1"
                     /note="T7 promoter color: #ffffff; direction: LEFT"
     promoter        2407..2484
                     /label=Promoter_P_2
                     /note="Promoter_P_2"
                     /note="lacI promoter color: #ffffff; direction: RIGHT"
     CDS             2485..3567
                     /codon_start=1
                     /label=CDS_7
                     /note="CDS_7"
                     /note="lacI color: #993366"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             4139..4330
                     /codon_start=1
                     /label=CDS_8
                     /note="CDS_8"
                     /note="rop color: #993366"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(4760..5348)
                     /direction=LEFT
                     /label=Rep_Origin_1
                     /note="Rep_Origin_1"
                     /note="ori color: #ffff00"
     CDS             5470..6285
                     /codon_start=1
                     /label=CDS_9
                     /note="CDS_9"
                     /note="KanR color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(6378..6833)
                     /direction=LEFT
                     /label=Rep_Origin_2
                     /note="Rep_Origin_2"
                     /note="f1 ori color: #ffff00"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgacg
      181 gatccactag ggactttaag cctctggttc tctgacacaa cttcatccag ctgccgcttc
      241 agtgttccat tttctttctt cagttgctcg ttttctgaga gggcagcctt taatctcgcc
      301 tctaacccta gcatatattc tttcttcttc ttgcgagact gacaagcgga ttctcgattt
      361 tttatcatac gttgctgtct ccttagcaca gcaatatctg aaccgacatt tctcatggta
      421 ctttgtagga caggtttagt cacggaaagt tttccattca ctgggctatt cgctgaaggg
      481 gctggtacca cattcaccac gtgattaggg agctgtgtga ctcccccagc aacagcaagg
      541 actggctgag cagagggcag aactccaggt gcttgaagtt gtaccacagt aggctgagac
      601 agcaaaaccg tctggccttt agtgggtgca ggttgtaaac tgataattgg ttgctgcttt
      661 gccaatggca taagcgttgg tactgtctga ataatgatgg tttttgctgg aacactggag
      721 tttgtttgag tcttgggtgc tgctggaagc aataaaggct tgggctgaat tgaaggtttt
      781 gaattcacct gaattttttt ctttggagtc agtccatttt cagtcttgtt cctaggacca
      841 gtgacaggct tatcttcctt cagtggctcc gctgaagaga gactattaga gttttcacca
      901 tataaggaaa ggggagacat ctgagaacta gaagacaaat ccaactcctc aggaacatgc
      961 tgagttgaag aataagagtc cactgaccga ggagatgaga ctgaataact tgaggaggct
     1021 ggagaaagtg gctggggttc tgccttaata tctttaactg tacagatttg gttgttgatg
     1081 tcccaaatgt ctgactccca aggcatcaaa tccaaatcaa aatcaagatt atcaaaattg
     1141 ttttcatacg tctcatttgc tgcttccaat tgcagctcat cagtgtctgt gaaataacca
     1201 agttcagcaa agagagcaga atcccaatct tcatccagcc tgtgaaagag tcccgggcta
     1261 aaaggtgact ccatggtgcc ggcaacccca gccggctccc ccatctcgag gggcccctgg
     1321 aacagaactt ccagactagt tgaaccatcc gattttggag gatggtcgcc accaccaaac
     1381 gtggcttgcc agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca
     1441 atttgtggga tagcttcaat acgtttttta aaacaaacta attttgggaa cgcatccagg
     1501 cacattgggt ccatgtataa aacaacatca agagcgtcat acaacatgaa gtcaggatgg
     1561 gttacatgat caccatttaa atatgtttta tgacataaac gatcttcgaa cattttcagc
     1621 atttcaggta gcttgctaag aaaatcaact ttgagagttt caaagtcttt actatatgca
     1681 attctcgaaa caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca
     1741 cgctcttttg gacaaccacc caacatgttg tgcttgtcag ctatataacg tatgatggcc
     1801 atagactgtg ttaatttaac atcaccatca atataataag gaagattggg aaactccaaa
     1861 cccaattcaa actttttgtt tcgccattta tcaccttcat cgcgctcata caaatgctct
     1921 tcatattttt cttcaagata ttccaaaaga agtcgagtgg gttgcacaag gcccttaatt
     1981 ttccaataac ctagtatagg ggacatatgt atatctcctt cttaaagtta aacaaaatta
     2041 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
     2101 cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     2161 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     2221 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     2281 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     2341 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
     2401 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     2461 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
     2521 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     2581 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     2641 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     2701 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     2761 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     2821 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     2881 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     2941 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
     3001 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     3061 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     3121 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     3181 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     3241 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc ggtagtggga
     3301 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
     3361 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     3421 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     3481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca
     3601 ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3661 gtgggcgcgg ggcatgacta gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     3721 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     3781 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     3841 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
     3901 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
     3961 ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
     4021 acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
     4081 tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt
     4141 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
     4201 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
     4261 tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
     4321 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4381 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     4441 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4501 attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4561 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5161 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     5221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5401 cacgttaagg gattttggtc atgaacaata aaactgtctg cttacataaa cagtaataca
     5461 aggggtgtta tgagccatat tcaacgggaa acgtcttgct ctaggccgcg attaaattcc
     5521 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     5581 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     5641 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     5701 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     5761 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
     5821 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     5881 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     5941 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     6001 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     6061 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     6121 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     6181 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     6241 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaagaatt aattcatgag
     6301 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6361 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     6421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     6481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     6541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     6601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     6661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     6721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     6781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     6841 gcca
//
