LOCUS       Exported                8529 bp ds-DNA     circular SYN 02-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 2, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(760..1554)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1555..1659)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1859..2363
                     /label=TUB1 upstream region
     CDS             2358..7415
                     /codon_start=1
                     /label=SthdCas9-CAT-BFP
                     /translation="MADYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAAHMSD
                     LVLGLAIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTNRQGRRLARRKKHR
                     RVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEELFIALKNMVKHRGISYL
                     DDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQLRGDFTVEKDGKKHRL
                     INVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKRKYYHGPGNEKSRTDYG
                     RYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLNDLNNLTVPTETKKLSK
                     EQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDKSGKAEIHTFEAYRKMK
                     TLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGSFSQKQVDELVQFRKAN
                     SSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTTSSSNKTKYIDEKLLTE
                     EIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDDEKKAIQKIQKANKDEK
                     DAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCLYTGKTISIHDLINNSN
                     QFEVDAILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDSMDDAWSFRELKAFVRE
                     SKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVLNALQEHFRAHKIDTKV
                     SVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLWKKQKNTLVSYSEDQLL
                     DIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQVDSKFNRKISDATIYA
                     TRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKFLMYRHDPQTFEKVIEP
                     ILENYPNKQINDKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPEIKSLKYYDSKLGNHID
                     ITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQFDKGTGTYKISQEKYND
                     IKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTMPKQKHYVELKPYDKQK
                     FEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQHIIKNEGDKPKLDFLEK
                     RPAATKKAGQAKKKKGSGATNFSLLKQAGDVEENPGPASGMHEKKITGYTTVDISQWHR
                     KEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAM
                     KDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPK
                     GFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAV
                     CDGFHVGRMLNELQQYCDEWQGGAGLQGSGEGRGSLLTCGDVEENPGPTSMSELIKENM
                     HMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKT
                     FINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFT
                     SNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKN
                     LKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN"
     CDS             2358..2360
                     /codon_start=1
                     /translation="M"
     CDS             2364..2429
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2436..2456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2484..5846
                     /codon_start=1
                     /label=Sth1 dCas9
                     /note="Sth1 dCas9"
                     /translation="MSDLVLGLAIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLV
                     RRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEE
                     LFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYG
                     QLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGK
                     RKYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLL
                     NDLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRID
                     KSGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADG
                     SFSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKT
                     TSSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNED
                     DEKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERC
                     LYTGKTISIHDLINNSNQFEVDAILPLSITFDDSLANKVLVYATANQEKGQRTPYQALD
                     SMDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVV
                     LNALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNL
                     WKKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSY
                     QVDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSK
                     FLMYRHDPQTFEKVIEPILENYPNKQINDKGKEVPCNPFLKYKEEHGYIRKYSKKGNGP
                     EIKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQ
                     FDKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRT
                     MPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQ
                     HIIKNEGDKPKLDF"
     CDS             5853..5900
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5910..5966
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5976..6635
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MHEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFS
                     SLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN
                     MDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6654..6707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6714..7415
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     3'UTR           7422..8164
                     /label=DHFR 3'UTR
     primer_bind     complement(8226..8242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt cagaagaact cgtcaagaag
      781 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
      841 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
      901 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
      961 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     1021 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     1081 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     1141 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     1201 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     1261 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     1321 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     1381 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     1441 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     1501 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatactctt
     1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1741 gaggcccttt cgtctcgcgc gtttcggtga tgacggtggt aaaacgacgg ccagtcaagg
     1801 cgattaagtt gggtaacgcc agggttttcc cagtcacgcg aattgggtac gcggccgcat
     1861 gcatgtcccg cgttcgtgaa attctctgca tcagcggagt gatcaggaat catcgtctca
     1921 gcgggatgac gttgcggagc aggccggctc gcggtgggca gtcagatgcc gaaggcgtaa
     1981 ctcaggacgg cttgcgctca tcgcagaaca ggggtggtgc ctgcattggg tgcggttggt
     2041 gatcctggtt ggaccggtgg agatgcgcgc gcacgaaggg gatgtgtcag aaacattttg
     2101 tttgttctct gtgaactttt agatgtgtta aaggcggcga atattagcag agagtcctcc
     2161 ttgttggatt ctctcttgaa tttcgccctt tccttctctt tgcgagtctc gtagagaaca
     2221 agcactcgtt cgccgtccct gacgacgcaa cccgcgcaga agacatccac caaacggtgt
     2281 tacacaatca ccttgtgtga agttcttgcg gaaaactact cgttggcatt ttttcttgaa
     2341 ttcccttttt cgacaaaatg gcggactata aggaccacga cggagactac aaggatcatg
     2401 atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta
     2461 tccacggagt cccagcagcc catatgtcgg acctggtgct gggcctggcc atcggcatcg
     2521 gctcggtggg cgtgggcatc ctgaacaagg tgaccggcga gatcatccac aagaactcgc
     2581 gcatcttccc ggccgcccag gccgagaaca acctggtgcg ccgcaccaac cgccagggcc
     2641 gccgcctggc ccgccgcaag aagcaccgcc gcgtgcgcct gaaccgcctg ttcgaggagt
     2701 cgggcctgat caccgacttc accaagatct cgatcaacct gaacccgtac cagctgcgcg
     2761 tgaagggcct gaccgacgag ctgtcgaacg aggagctgtt catcgccctg aagaacatgg
     2821 tgaagcaccg cggcatctcg tacctggacg acgcctcgga cgacggcaac tcgtcggtgg
     2881 gcgactacgc ccagatcgtg aaggagaact cgaagcagct ggagaccaag accccgggcc
     2941 agatccagct ggagcgctac cagacctacg gccagctgcg cggcgacttc accgtggaga
     3001 aggacggcaa gaagcaccgc ctgatcaacg tgttcccgac ctcggcctac cgctcggagg
     3061 ccctgcgcat cctgcagacc cagcaggagt tcaacccgca gatcaccgac gagttcatca
     3121 accgctacct ggagatcctg accggcaagc gcaagtacta ccacggcccg ggcaacgaga
     3181 agtcgcgcac cgactacggc cgctaccgca cctcgggcga gaccctggac aacatcttcg
     3241 gcatcctgat cggcaagtgc accttctacc cggacgagtt ccgcgccgcc aaggcctcgt
     3301 acaccgccca ggagttcaac ctgctgaacg acctgaacaa cctgaccgtg ccgaccgaga
     3361 ccaagaagct gtcgaaggag cagaagaacc agatcatcaa ctacgtgaag aacgagaagg
     3421 ccatgggccc ggccaagctg ttcaagtaca tcgccaagct gctgtcgtgc gacgtggccg
     3481 acatcaaggg ctaccgcatc gacaagtcgg gcaaggccga gatccacacc ttcgaggcct
     3541 accgcaagat gaagaccctg gagaccctgg acatcgagca gatggaccgc gagaccctgg
     3601 acaagctggc ctacgtgctg accctgaaca ccgagcgcga gggcatccag gaggccctgg
     3661 agcacgagtt cgccgacggc tcgttctcgc agaagcaggt ggacgagctg gtgcagttcc
     3721 gcaaggccaa ctcgtcgatc ttcggcaagg gctggcacaa cttctcggtg aagctgatga
     3781 tggagctgat cccggagctg tacgagacct cggaggagca gatgaccatc ctgacccgcc
     3841 tgggcaagca gaagaccacc tcgtcgtcga acaagaccaa gtacatcgac gagaagctgc
     3901 tgaccgagga gatctacaac ccggtggtgg ccaagtcggt gcgccaggcc atcaagatcg
     3961 tgaacgccgc catcaaggag tacggcgact tcgacaacat cgtgatcgag atggcccgcg
     4021 agaccaacga ggacgacgag aagaaggcca tccagaagat ccagaaggcc aacaaggacg
     4081 agaaggacgc cgccatgctg aaggccgcca accagtacaa cggcaaggcc gagctgccgc
     4141 actcggtgtt ccacggccac aagcagctgg ccaccaagat ccgcctgtgg caccagcagg
     4201 gcgagcgctg cctgtacacc ggcaagacca tctcgatcca cgacctgatc aacaactcga
     4261 accagttcga ggtggacgcc atcctgccgc tgtcgatcac cttcgacgac tcgctggcca
     4321 acaaggtgct ggtgtacgcc accgccaacc aggagaaggg ccagcgcacc ccgtaccagg
     4381 ccctggactc gatggacgac gcctggtcgt tccgcgagct gaaggccttc gtgcgcgagt
     4441 cgaagaccct gtcgaacaag aagaaggagt acctgctgac cgaggaggac atctcgaagt
     4501 tcgacgtgcg caagaagttc atcgagcgca acctggtgga cacccgctac gcctcgcgcg
     4561 tggtgctgaa cgccctgcag gagcacttcc gcgcccacaa gatcgacacc aaggtgtcgg
     4621 tggtgcgcgg ccagttcacc tcgcagctgc gccgccactg gggcatcgag aagacccgcg
     4681 acacctacca ccaccacgcc gtggacgccc tgatcatcgc cgcctcgtcg cagctgaacc
     4741 tgtggaagaa gcagaagaac accctggtgt cgtactcgga ggaccagctg ctggacatcg
     4801 agaccggcga gctgatctcg gacgacgagt acaaggagtc ggtgttcaag gccccgtacc
     4861 agcacttcgt ggacaccctg aagtcgaagg agttcgagga ctcgatcctg ttctcgtacc
     4921 aggtggactc gaagttcaac cgcaagatct cggacgccac catctacgcc acccgccagg
     4981 ccaaggtggg caaggacaag gccgacgaga cctacgtgct gggcaagatc aaggacatct
     5041 acacccagga cggctacgac gccttcatga agatctacaa gaaggacaag tcgaagttcc
     5101 tgatgtaccg ccacgacccg cagaccttcg agaaggtgat cgagccgatc ctggagaact
     5161 acccgaacaa gcagatcaac gacaagggca aggaggtgcc gtgcaacccg ttcctgaagt
     5221 acaaggagga gcacggctac atccgcaagt actcgaagaa gggcaacggc ccggagatca
     5281 agtcgctgaa gtactacgac tcgaagctgg gcaaccacat cgacatcacc ccgaaggact
     5341 cgaacaacaa ggtggtgctg cagtcggtgt cgccgtggcg cgccgacgtg tacttcaaca
     5401 agaccaccgg caagtacgag atcctgggcc tgaagtacgc cgacctgcag ttcgacaagg
     5461 gcaccggcac ctacaagatc tcgcaggaga agtacaacga catcaagaag aaggagggcg
     5521 tggactcgga ctcggagttc aagttcaccc tgtacaagaa cgacctgctg ctggtgaagg
     5581 acaccgagac caaggagcag cagctgttcc gcttcctgtc gcgcaccatg ccgaagcaga
     5641 agcactacgt ggagctgaag ccgtacgaca agcagaagtt cgagggcggc gaggccctga
     5701 tcaaggtgct gggcaacgtg gccaactcgg gccagtgcaa gaagggcctg ggcaagtcga
     5761 acatctcgat ctacaaggtg cgcaccgacg tgctgggcaa ccagcacatc atcaagaacg
     5821 agggcgacaa gccgaagctg gacttcctcg agaaaaggcc ggcggccacg aaaaaggccg
     5881 gccaggcaaa aaagaaaaag ggaagcggag ctactaactt cagcctgctg aagcaggctg
     5941 gagacgtgga ggagaaccct ggacctgcta gcggaatgca tgagaaaaaa atcactggat
     6001 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     6061 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     6121 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     6181 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     6241 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     6301 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     6361 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     6421 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     6481 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     6541 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     6601 tacaacagta ctgcgatgag tggcagggcg gggctggcct gcagggaagc ggagagggca
     6661 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctact agtatgagcg
     6721 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     6781 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     6841 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     6901 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     6961 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     7021 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     7081 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     7141 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     7201 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     7261 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     7321 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     7381 acctccctag caaactgggg cacaagctta attaaggatc cacagaagct gcccgtctct
     7441 cgttttcctc tcttttcgga gggatcaggg agagtgcctc gggtcggaga gagctgacga
     7501 gggggtgcca gagacccctg tgtcctttat cgaagaaaag ggatgactct tcatgtggca
     7561 tttcacacag tctcacctcg ccttgttttc tttttgtcaa tcagaacgaa agcgagttgc
     7621 gggtgacgca gatgtgcgtg tatccactcg ngaatgcgtt atcgttctgt atgccgctag
     7681 agtgctggac tgttgctgtc tgcccacgac agcagacaac tttccttcta tgcacttgca
     7741 ggatggtgca gcgcaaacga cggagagaaa ggagcaccct ctcagtttcc ctacgatgtg
     7801 ctgtcagttt cgactcttca ccgcgaacga ttggcgatac gtctctgttg acttgttagg
     7861 ctccgaccac gaagctccct taactagata agccgcgaca cctaagtgta caccatttgc
     7921 agatcgataa tctgcgaccg ctgaatccgt ccagatcagt aaaaccgcac cacctaagtg
     7981 taaaccttgt ttaggtcgat aaaatgctac caacccccac ccacaatcga gccttgagcg
     8041 tttctgcgca cgcgttggcc tacgtgactt gctgatgcct gcctctggcc attcatgcca
     8101 gtcagtgcgc ataaaaatgt ggacacagtc ggttgacaag tgttctggca ggctacagtg
     8161 acaccaattg tctggacggt cgacggtatc gataagcttg taacttgttt aatcatctca
     8221 cagtggtcat agctgtttcc tgattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8521 gctggcgtt
//
